Initial Glutathione Depletion During Short-Term Bed Rest: Pinpointing Synthesis and Degradation Checkpoints in the γ-Glutamyl Cycle DOI Creative Commons
Filippo Giorgio Di Girolamo, Filippo Mearelli,

Mariella Sturma

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1430 - 1430

Published: Nov. 21, 2024

Hypokinesia triggers oxidative stress and accelerates the turnover of glutathione system via γ-glutamyl cycle. Our study aimed to identify regulatory checkpoints controlling intracellular levels. We measured intermediate substrates cycle in erythrocytes from 19 healthy young male volunteers before during a 10-day experimental bed rest. Additionally, we tracked changes levels specific metabolite ratios up 21 days Using gas chromatography-mass spectrometry internal standard technique, observed 9 ± 9% decrease first 5 rest, followed by an 11 increase 5th 10th day, nearly returning baseline ambulatory The cysteinyl-glycine-to-glutathione ratio, reflecting cyclotransferase activity (a key enzyme breakdown), rose 14 22% then fell 10 14% over subsequent days, again approaching cysteine-to-cysteine indicative cysteine synthetase (crucial for synthesis), increased 12 30% on day 29 41% results rest confirm those seen 10. By calculating ratio product concentration precursor concentration, assessed efficiency these enzymes turnover. These were corroborated directly measuring synthesis degradation rates vivo using stable isotope techniques. findings reveal significant kinetics initial potential therapeutic targets maintaining

Language: Английский

Indole-3-Carbinol and Its Derivatives as Neuroprotective Modulators DOI Creative Commons
Alka Ashok Singh, Dhananjay Yadav, Fazlurrahman Khan

et al.

Brain Sciences, Journal Year: 2024, Volume and Issue: 14(7), P. 674 - 674

Published: July 2, 2024

Brain-derived neurotrophic factor (BDNF) and its downstream tropomyosin receptor kinase B (TrkB) signaling pathway play pivotal roles in the resilience action of antidepressant drugs, making them prominent targets psychiatric research. Oxidative stress (OS) contributes to various neurological disorders, including neurodegenerative diseases, stroke, mental illnesses, exacerbates aging process. The nuclear erythroid 2-related 2 (Nrf2)-antioxidant responsive element (ARE) serves as primary cellular defense mechanism against OS-induced brain damage. Thus, Nrf2 activation may confer endogenous neuroprotection OS-related damage; notably, TrkB/phosphoinositide 3-kinase (PI3K)/protein (Akt) pathway, stimulated by BDNF-dependent TrkB signaling, activates promotes translocation. However, insufficient neurotrophin support often leads downregulation diseases. targeting Nrf2-ARE system is a promising therapeutic strategy for treating Phytochemicals, indole-3-carbinol (I3C) metabolite, diindolylmethane (DIM), exhibit neuroprotective effects through BDNF’s mimetic activity; Akt phosphorylation induced, antioxidant activated blocking Nrf2-kelch-like ECH-associated protein 1 (Keap1) complex. This review emphasizes potential I3C derivatives concurrently activating neuronal mechanisms treatment

Language: Английский

Citations

8
The role of potential oxidative biomarkers in the prognosis of intracerebral hemorrhage and the exploration antioxidants as possible preventive and treatment options DOI Creative Commons

Jiayong Yao,

Xiaohong Dai,

Xueping Yv

et al.

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 4, 2025

Intracerebral hemorrhage (ICH) is a non traumatic that occurs in certain part of the brain. It usually leads to brain cell damage. According large number experimental research, oxidative stress an important pathophysiological processes cerebral hemorrhage. In this paper, we aim determine how changes biomarkers indicate damage degree hemorrhage, and explore summarize potential treatments or interventions. We found patients with are characterized by increased levels markers, such as total malondialdehyde (MDA), F2 isoprostaglandin, hydroxynonenal, myeloperoxidase protein hydroxyl. Therefore, caused ICH on these markers can be used evaluate diagnose ICH, predict its prognosis, guide preventive treatment turn antioxidant based new alternative.

Language: Английский

Citations

0
The relationship between physical activity and telomere length in women: A systematic review DOI

Jeni Page,

Christine Stephens, Melissa A. Richard

et al.

Mechanisms of Ageing and Development, Journal Year: 2025, Volume and Issue: unknown, P. 112042 - 112042

Published: Feb. 1, 2025

Language: Английский

Citations

0
Development and application of a Phenothiazine-Based fluorescent probe for distinguishing hydrogen sulfide and hypochlorite in oxidative stress DOI
Jiaxue Yang,

San Tu,

Tingting Feng

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113160 - 113160

Published: Feb. 1, 2025

Language: Английский

Citations

0
Unveiling the geroprotective potential of Monarda didyma L.: insights from in vitro studies and a randomized clinical trial on slowing biological aging and improving quality of life DOI Creative Commons
Manuela Campisi, Luana Cannella,

Omar Paccagnella

et al.

GeroScience, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Abstract Aging is driven by fundamental mechanisms like oxidative stress, telomere shortening and changes in DNA methylation, which together prepare the ground for age-related diseases. Botanical extracts, rich bioactive phytoconstituents, represent a promising resource developing therapies that target these to promote healthy aging. This study explores geroprotective potential of Monarda didyma L. extract. In vitro analyses revealed extract’s strong antioxidant activity, ability reduce shortening, capacity protect against damage, thereby decreasing cellular senescence improving endothelial function. The randomized, double-blind clinical trial demonstrated daily oral supplementation with extract significantly improved leukocyte length (LTL) stabilized methylation age (DNAmAge) intervention group, while placebo group experienced accelerated epigenetic aging hypermethylation critical genes (ELOVL2 FHL2). also reported enhanced quality life, particularly physical domain, along movement sleep indices detected questionnaire wearable sensors. These compelling findings position as powerful candidate future therapies, impact

Language: Английский

Citations

0
Non high density lipoprotein to high density lipoprotein cholesterol ratio and type 2 diabetes in Middle aged and Elderly Chinese DOI Creative Commons

Jiacong Tan,

Huaxin Zhu,

Yanyang Zeng

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 12, 2025

The aim of this study was to assess the potential association between non-high-density lipoprotein cholesterol high-density ratio (NHHR) and type 2 diabetes mellitus (T2DM) in a middle-aged elderly Chinese population using data from China Health Retirement Longitudinal Study (CHARLS) 2011 2015. Methods We used CHARLS as baseline follow-up NHHR continuous categorical variable, multivariate logistic regression modeling explore its relationship with T2DM. Three models were developed adjust for possible effects 14 factors on outcomes. Restricted cubic spline check nonlinear associations, subgroup interaction analyses differences groups. Results A total 7847 subjects enrolled study, whom 948 (12.1%) diagnosed last quartile group (Q4) presented highest risk T2DM (OR, 1.115, 95% CI, 1.088–1.141) after accounting all covariates. revealed (p = 0.001). results consistent across categories, indicating significant positive correlation. Interaction interactions age, gender marital status. Conclusion In China, there is strong correlation elevated increased onset. can be further investigated future provide reference development more accurate prediction models.

Language: Английский

Citations

0
Relationship between physical activity and DNA methylation-predicted epigenetic clocks DOI Creative Commons
Yanwei You, Yuquan Chen, Hao Ding

et al.

npj Aging, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 12, 2025

This study investigates the relationship between physical activity (PA) levels and DNA methylation (DNAm)-predicted epigenetic clocks in a U.S. population sample (n = 948, mean age 62, 49% female). Eight were analyzed, revealing that higher PA significantly associated with younger biological ages across all indicators, strongest effects observed for SkinBloodAge LinAge. Multivariable linear regression models, adjusted sociodemographic lifestyle factors, highlighted potential of to reduce ageing. Subgroup analyses indicated these associations more pronounced among non-Hispanic whites, individuals BMI 25-30, former smokers, suggesting impact varies different groups. These findings emphasize role slowing ageing reducing age-related health risks. Promoting regular PA, especially older adults those BMI, could improve well-being lifespan, highlighting as modifiable factor healthy disease prevention.

Language: Английский

Citations

0
Beneficial effects of a combined lifestyle intervention for older people in a long-term-care facility on redox balance and endothelial function DOI Creative Commons
Elena Grossini,

Sakthipryian Venkatesan,

Mohammad Mostafa Ola Pour

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(16), P. e35850 - e35850

Published: Aug. 1, 2024

It has recently been highlighted how a short healthy life-style program (LSP) can improve the functional outcomes of older people admitted to Long-Term Care (LTC) facility. Although it is known that medicine-based interventions exert anti-aging effects through modulation oxidative stress and mitochondrial function, mechanisms underlying aforementioned have not clarified, yet. For this reason, in study, were focused on investigation possible benefits LSP people. This was achieved by examining circulating markers immunosenescence, such as Tymosin β (Tβ4), before after plasma undergone or endothelial cells.

Language: Английский

Citations

2
Association between the Planetary Health Diet Index and biological aging among the U.S. population DOI Creative Commons

Shaoqun Huang,

H. M. Hu,

Hongyang Gong

et al.

Frontiers in Public Health, Journal Year: 2024, Volume and Issue: 12

Published: Oct. 22, 2024

Background The Planetary Health Diet (PHD) is a novel dietary pattern proposed by the EAT-Lancet Commission in 2019, yet limited study has investigated anti-aging effects of PHD to date. Objectives This aimed explore association between adherence PHD, as quantified Index (PHDI), and biological aging American populations. Methods Data were obtained from National Nutrition Examination Survey (NHANES) for 1999–2018. Food consumption information was relied on two 24-h diet recall questionnaires. condition comprehensively assessed four markers, including phenotypic age, telomere length, klotho concentration. Weighted multivariate linear models, restricted cubic spline (RCS), subgroup analysis subsequently carried out evaluate influence PHDI aging. Results 44,925 participants with complete data finally enrolled our study. fully adjusted models showed decreased 0.20 years age [−0.20 (−0.31, −0.10)] declined 0.54 [−0.54 (−0.69, −0.38)] correlated per 10 scores increment. Klotho concentration [6.2 (1.0, 11.0)] positively related PHDI. In Model 2, length increased 0.02 bp every 10-point rise Besides, RCS results exhibited curvilinear relationship indicators. Conclusion Our explored significant correlation aging, indicating that may prevent

Language: Английский

Citations

2
Exercise-mediated circulating metabolites for brain health DOI Creative Commons
Minghui Wang, Mohammad Nasb, Tong Wu

et al.

Advanced Exercise and Health Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

2