Molecular Chaperonin HSP60: Current Understanding and Future Prospects

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5483 - 5483

Published: May 17, 2024

Molecular chaperones are highly conserved across evolution and play a crucial role in preserving protein homeostasis. The 60 kDa heat shock (HSP60), also referred to as chaperonin (Cpn60), resides within mitochondria is involved maintaining the organelle’s proteome integrity HSP60 family, encompassing Cpn60, plays diverse roles cellular processes, including folding, cell signaling, managing high-temperature stress. In prokaryotes, well understood GroEL/GroES complex, which forms double-ring cavity aids folding. eukaryotes, implicated numerous biological functions, like facilitating folding of native proteins influencing disease development processes. Notably, research highlights its critical involvement sustaining oxidative stress mitochondrial integrity. perturbation results loss activates apoptosis. Currently, clinical investigations progress explore targeting both vivo vitro various models. These studies aim enhance our comprehension mechanisms potentially harness therapeutic target for conditions, cancer, inflammatory disorders, neurodegenerative diseases. This review delves into functions regulating proteo-homeostasis, stress, ROS, apoptosis, implications diseases cancer neurodegeneration.

Language: Английский

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Stem Cell Therapy for Diseases of Livestock Animals: An In-Depth Review

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(1), P. 67 - 67

Published: Jan. 17, 2025

Stem cells are unique, undifferentiated that have the ability to both replicate themselves and develop into specialized cell types. This dual capability makes them valuable in development of regenerative medicine. Current stem research has widened their application therapy, drug discovery, reproductive cloning animals, models for various diseases. Although there substantial studies revealing treatment human degenerative diseases using cells, this is yet be explored livestock animals. Many species such as mastitis, laminitis, neuromuscular disorders, autoimmune diseases, some debilitating not covered completely by existing drugs can improved different types like embryonic adult induced pluripotent cells. review mainly focuses on use disease In addition mentioned, potential helpful wound healing, skin genetic disorders. article explores from sources therapy also role conservation endangered well model preparation. Moreover, future perspectives challenges associated with discussed. Overall, transformative impact sector comprehensively studied which will help researchers design work related

Language: Английский

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Role of HSF1 in cell division, tumorigenesis and therapy: a literature review

Cell Division, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 26, 2025

Abstract Heat shock factor 1 (HSF1) is the master orchestrator of heat response (HSR), a critical process for maintaining cellular health and protein homeostasis. These effects are achieved through rapid expression molecular chaperones, proteins (HSPs), which ensure correct folding, repair, degradation stabilization multiprotein complexes. In addition to its role in HSR, HSF1 influences cell cycle, including processes such as S phase progression regulation p53 pathway, highlighting importance synthesis division. While activity offers neuroprotective benefits neurodegenerative diseases, proteome-stabilizing function may also reinforce tumorigenic transformation. overexpression many types cancer reportedly enhances growth enables survival, alters metabolism, weakens immune promotes angiogenesis or epithelial-mesenchymal transition (EMT) these cells enter form “HSF1 addiction”. Furthermore, client HSF1-regulated particularly Hsp90, include numerous key players classical pathways. thus presents promising therapeutic target treatment, potentially combination with HSP inhibitors alleviate typical initiation HSR upon their use.

Language: Английский

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Extracellular vesicles enabling moonlighting proteins

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 11 - 29

Published: Jan. 1, 2025

Language: Английский

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Toxoplasma gondii infection of neurons alters the production and content of extracellular vesicles altering astrocyte phenotype and contributing to the loss of GLT-1 in the infected brain

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Abstract Toxoplasma gondii (T. gondii) cyst formation in the central nervous system only occurs neurons allowing parasite to remain latent for lifetime of host. Astrocytes are fundamental neuronal health by providing nutrients and structural support help regulate neurotransmitters continuous communication with neurons. It is not yet known how infection presence intracellular cysts, disrupts crucial relationship between these cells. Extracellular vesicles (EVs) function can contain proteins, lipids, DNA, miRNA, other RNA subtypes. EVs produced all cells including play an important role neuronal-astrocyte interactions regulation glutamate receptors on astrocytes. Previous work has demonstrated reduces astrocytic expression primary transporter, GLT-1. Here we tested if alters production content EVs. were isolated from uninfected infected murine cortical their size, concentration, characterization confirmed nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), CD63 ELISA, liquid chromatography (LC)-mass spectrometry (MS)/MS, microRNA Sequencing. Analysis reveals that reduced altered protein miRNA content. contained secreted proteins GRA1, GRA2, GRA7, MAG1 MAG2 associated formation. Following incubation astrocytes, a proportion colocalize nucleus. gene astrocytes leading downregulation GLT-1 increase pro-inflammatory transcriptional signatures. These results demonstrate ability parasitic brain alter EV

Language: Английский

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In silico studies of green fluorescent protein-tagged Hsp27-HPV16 E7 fusion protein and evaluation of cytokine secretion from antigen-presenting cells exposed to the fusion protein-carrying tumor-derived exosomes

Journal of Solid Tumors, Journal Year: 2024, Volume and Issue: 14(1), P. 45 - 45

Published: Dec. 15, 2024

Objective: Exosome (Exo)-based therapies have attracted considerable interest due to their potential as carriers for therapeutic molecules and capacity elicit anti-tumor immune responses. The objective of this study was engineer TC-1 tumor cell line-derived exosomes with GFP-tagged heat shock protein (Hsp) 27-human papillomavirus (HPV)16 E7 fusion evaluation cytokine secretion from antigen-presenting cells (APCs: macrophages dendritic cells) exposed the engineered in vitro.Methods: In study, different silico methods were employed evaluate Hsp27-E7 Hsp27-E7-GFP proteins vaccine candidates. Regarding data, gene subcloned into pCDH lentiviral vector production lentivirions harboring eukaryotic cells. Subsequently, transduced these isolate (i.e., Exo-Hsp27-E7-GFP) using ExoQuick-TCTM kit characterization physicochemical methods. Finally, key cytokines (IFN-γ, TNF-α, IL-10) evaluated through incubation (APCs) Exo-Hsp27-E7-GFP enzyme-linked immunosorbent assay (ELISA).Results: Our data showed that both constructs soluble non-allergenic, exhibited strong interaction TLR4. Indeed, linkage GFP did not affect properties, receptors. Moreover, western blot analysis confirmed presence isolated exosomes. could significantly enhance TNF-α IL-10 APCs compared Exo Exo-GFP, well.Conclusions: These vesicles derived demonstrate induce effective immunity, suggesting a promising strategy development cell-free

Language: Английский

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Systematic characterization of extracellular vesicles from potato (Solanum tuberosum cv. Laura) roots and peels: biophysical properties and proteomic profiling

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 15, 2024

Introduction Extracellular vesicles (EVs) facilitate inter and intra-species/kingdom communication through biomolecule transfer, including proteins small RNAs. Plant-derived EVs, a hot topic in the field, hold immense capability both as potential biomarker to study plant physiology biomaterial that can be mass-produced used various industries ranging from cosmetics food additives biological pesticides. However, systematic characterization of EVs is required establish foundation for further applications studies. Methods In this study, were enriched hydroponically cultivated potato plants ( Solanum tuberosum , cv. Laura). We isolated root exudates apoplastic wash peels using vacuum infiltration. These then systematically characterized their biophysical chemical properties compare with standard EV characteristics explore roles physiology. Results Biophysical analyses revealed morphological similarities between peel-derived EVs. The average diameter root-derived (164.6 ± 7.3 nm) was significantly larger than (132.2 2.0 nm, p < 0.004). Liquid chromatography–mass spectrometry (LC-MS) demonstrated substantial protein enrichment purified compared crude samples, 42% 25% peel Only 11.8% identified common just 2% shared. Enriched pathways proteomes associated responses biotic abiotic stress, suggesting defensive role plants. Discussion With experimentation elucidate specific methods communication, these findings increase details known about terms physical functions, aiding sustainable agricultural waste utilization large-scale production, aligning concept “valorization”.

Language: Английский

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