An azo-Schiff base chemosensor for selective turn-on fluorescent sensing of Al3+ in antacid tablets and cell-imaging applications

Journal of Photochemistry and Photobiology A Chemistry, Journal Year: 2024, Volume and Issue: 451, P. 115532 - 115532

Published: Feb. 14, 2024

Language: Английский

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Development of a water-soluble fluorescent Al3+ probe based on phenylsulfonyl-2-pyrone in biological systems

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1299, P. 342436 - 342436

Published: Feb. 29, 2024

Language: Английский

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Design strategies for organelle-selective fluorescent probes: where to start?

RSC Advances, Journal Year: 2025, Volume and Issue: 15(3), P. 2115 - 2131

Published: Jan. 1, 2025

Advancing the development of organelle-targeted small fluorescent probes by highlighting key design strategies to reveal cellular dynamics and address persistent challenges in probe design.

Language: Английский

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In silico study of a new class of DNA fluorescent probes: docking, molecular dynamics and quantum chemistry calculations

Journal of Molecular Modeling, Journal Year: 2025, Volume and Issue: 31(4)

Published: April 1, 2025

Language: Английский

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Fluorescent probes in autoimmune disease research: current status and future prospects

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 9, 2025

Autoimmune diseases (AD) present substantial challenges for early diagnosis and precise treatment due to their intricate pathogenesis varied clinical manifestations. While existing diagnostic methods strategies have advanced, sensitivity, specificity, real-time applicability in settings continue exhibit significant limitations. In recent years, fluorescent probes emerged as highly sensitive specific biological imaging tools, demonstrating potential AD research.This review examines the response mechanisms historical evolution of various types probes, systematically summarizing latest research advancements application autoimmune diseases. It highlights key applications biomarker detection, dynamic monitoring immune cell functions, assessment drug efficacy. Furthermore, this article analyzes technical currently encountered probe development proposes directions future research. With ongoing materials science, nanotechnology, bioengineering, are anticipated achieve higher sensitivity enhanced functional integration, thereby facilitating monitoring, innovative Overall, possess scientific significance value both related diseases, signaling a new era personalized precision medicine.

Language: Английский

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A biocompatible NIR squaraine dye and dye-antibody conjugates for versatile long-term in vivo fluorescence bioimaging

Materials Advances, Journal Year: 2024, Volume and Issue: 5(9), P. 3940 - 3949

Published: Jan. 1, 2024

In vivo studies demonstrate SQ-58's biocompatibility for long-term NIR imaging and targeting specific tumors.

Language: Английский

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Quantitative imaging and semiotic phenotyping of mitochondrial network morphology in live human cells

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(3), P. e0301372 - e0301372

Published: March 28, 2024

The importance of mitochondria in tissue homeostasis, stress responses and human diseases, combined to their ability transition between various structural functional states, makes them excellent organelles for monitoring cell health. There is therefore a need technologies accurately analyze quantify changes mitochondrial organization variety cells cellular contexts. Here we present an innovative computerized method that enables accurate, multiscale, fast cost-effective analysis shape network architecture from confocal fluorescence images by providing more than thirty features. In order facilitate interpretation the quantitative results, introduced two innovations: use Kiviat-graphs (herein named MitoSpider plots) highly multidimensional data visualization mito-cellular configurations form morphospace diagrams (called MitoSigils). We tested our fully automated image tool on rich datasets gathered live normal skin cultured under basal conditions or exposed specific including UVB irradiation pesticide exposure. demonstrated proprietary software (named MitoTouch) sensitively discriminate control stressed dermal fibroblasts, fibroblasts other types (including cancer tissue-derived primary keratinocytes), showing captures subtle differences morphology. Based this novel algorithm, report identification protective natural ingredient mitigates deleterious impact hydrogen peroxide (H2O2) organization. Hence conceived wet-plus-dry pipeline combining cultures, imaging semiotic exhaustive morphology living adherent cells. Our has potential broader applications research areas such as biology medicine, high-throughput drug screening well predictive environmental toxicology.

Language: Английский

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Flaring Inflammation and ER Stress by an Organelle‐Specific Fluorescent Cage

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 7, 2024

Abstract The endoplasmic reticulum (ER) plays an important role in protein synthesis and its disruption can cause unfolding misfolding. Accumulation of such proteins leads to ER stress, which ultimately promotes many diseases. Routine screening activity immune cells flag serious conditions at early stages, but the current clinically used bio‐probes have limitations. Herein, ER‐specific fluorophore based on a biocompatible benzothiadiazole‐imine cage (BTD‐cage) with excellent photophysical properties is developed. outperforms commercially available stains long‐term live cell imaging no fading or photobleaching over time. responsive different levels stress where fluorescence increases accordingly. Incorporating bio‐probe into disorder model, 6‐, 21‐, 48‐fold increase intensity shown THP‐1, Raw 246.7, Jurkat cells, respectively (within 15 min). These results strongly support that this system be for rapid visual selective detection stress. It envisaged tailoring molecular interactions recognition using supramolecular improved fluorophores expand library biological probes enhanced selectivity targetability toward cellular organelles.

Language: Английский

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Quantitative Structure-Activity Relationship of Fluorescent Probes and Their Intracellular Localizations

Chemosensors, Journal Year: 2023, Volume and Issue: 11(5), P. 310 - 310

Published: May 22, 2023

The development of organelle-specific fluorescent probes has been impeded by the absence a comprehensive understanding relationship between physicochemical properties and their selectivity towards specific organelles. Although few machine learning models have suggested several parameters that control target organelle attempted to predict organelles, they challenged low accuracy limited range applicable Herein, we report multi-organelle prediction QSAR model is capable predicting destination among nine categories, including cytosol, endoplasmic reticulum, Golgi body, lipid droplet, lysosome, mitochondria, nucleus, plasma membrane, no entry. trained using Random Forest algorithm with dataset 350 786 descriptors, it able organelles an 75%. MDI analysis identifies 38 key significant impact on probes, LogD, pKa, hydrophilic-lipophilic balance (HLB), topological polar surface area (TPSA). This may be useful in developing new providing crucial variables determine probes.

Language: Английский

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Editorial on the Special Issue “Fluorescence Imaging and Analysis of Cellular Systems”

Journal of Imaging, Journal Year: 2024, Volume and Issue: 10(11), P. 293 - 293

Published: Nov. 18, 2024

Fluorescence imaging has indeed become a cornerstone in modern cell biology due to its ability offer highly sensitive, specific, and real-time visualization of cellular structures dynamic processes [...].

Language: Английский

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