Applications of Tandem Mass Spectrometry (MS/MS) in Protein Analysis for Biomedical Research

Molecules, Journal Year: 2022, Volume and Issue: 27(8), P. 2411 - 2411

Published: April 8, 2022

Mass Spectrometry (MS) allows the analysis of proteins and peptides through a variety methods, such as Electrospray Ionization-Mass (ESI-MS) or Matrix-Assisted Laser Desorption (MALDI-MS). These methods allow identification mass protein peptide intact molecules peptide-mass fingerprinting generated upon enzymatic digestion. Tandem spectrometry (MS/MS) fragmentation to determine amino acid sequence (top-down middle-down proteomics) (bottom-up proteomics). Furthermore, tandem also post-translational modifications (PTMs) peptides. Here, we discuss application MS/MS in biomedical research, indicating specific examples for their PTMs relevant biomarkers diagnostic therapy.

Language: Английский

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The roles of E3 ubiquitin ligases in cancer progression and targeted therapy

Clinical and Translational Medicine, Journal Year: 2023, Volume and Issue: 13(3)

Published: March 1, 2023

Abstract Ubiquitination is one of the most important post‐translational modifications which plays a significant role in conserving homeostasis cellular proteins. In ubiquitination process, ubiquitin conjugated to target protein substrates for degradation, translocation or activation, dysregulation linked several diseases including various types cancers. E3 ligases are regarded as influential enzyme owing their ability select, bind and recruit ubiquitination. particular, pivotal cancer hallmarks pathways where they serve tumour promoters suppressors. The specificity coupled with implication engendered development compounds that specifically therapy. this review, we highlight such sustained proliferation via cell cycle progression, immune evasion promoting inflammation, apoptosis. addition, summarise application small treatment along significance targeting potential

Language: Английский

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The evolution of post-translational modifications

Current Opinion in Genetics & Development, Journal Year: 2022, Volume and Issue: 76, P. 101956 - 101956

Published: July 13, 2022

Language: Английский

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Western Blotting: A Powerful Staple In Scientific and Biomedical Research

BioTechniques, Journal Year: 2022, Volume and Issue: 73(1), P. 58 - 69

Published: July 1, 2022

Western blotting (WB), also known as immunoblotting, is a well-known molecular biology method that biologists often use to investigate many features of the protein, ranging from basic protein analysis disease detection. WB simple, unique, rapid, widely used routine tool with easy interpretation and definite results. It being in various fields science, research development, diagnostic labs hospitals. The principle accomplish separation proteins based on weight charge. This review addresses detail individual steps involved technique, its troubleshooting, internal loading controls, total staining diverse applications scientific clinical settings, along future perspectives.

Language: Английский

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Advances in enrichment methods for mass spectrometry-based proteomics analysis of post-translational modifications

Journal of Chromatography A, Journal Year: 2022, Volume and Issue: 1678, P. 463352 - 463352

Published: July 18, 2022

Language: Английский

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Proteomics of post-translational modifications in colorectal cancer: Discovery of new biomarkers

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2022, Volume and Issue: 1877(4), P. 188735 - 188735

Published: May 13, 2022

Language: Английский

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Deciphering combinatorial post-translational modifications by top-down mass spectrometry

Current Opinion in Chemical Biology, Journal Year: 2022, Volume and Issue: 70, P. 102180 - 102180

Published: June 29, 2022

Language: Английский

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An inventory of crosstalk between ubiquitination and other post-translational modifications in orchestrating cellular processes

iScience, Journal Year: 2023, Volume and Issue: 26(5), P. 106276 - 106276

Published: Feb. 26, 2023

Ubiquitination is an important post-translational modification (PTM) that regulates a large spectrum of cellular processes in eukaryotes. Abnormalities ubiquitin signaling underlie numerous human pathologies including cancer and neurodegeneration. Much progress has been made during the last three decades understanding how ligases recognize their substrates ubiquitination orchestrated. Several mechanisms regulation have evolved to prevent promiscuity assembly multi-protein complexes with dedicated subunits specific modifications these enzymes co-factors. Here, we outline another layer complexity involving coordinated access E3 substrates. We provide extensive inventory crosstalk multiple PTMs SUMOylation, phosphorylation, methylation, acetylation, hydroxylation, prolyl isomerization, PARylation, O-GlcNAcylation. discuss molecular by which orchestrate ubiquitination, thus increasing its specificity as well other pathways ensure cell homeostasis.

Language: Английский

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Lactylome analyses suggest systematic lysine-lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia

Cellular Signalling, Journal Year: 2024, Volume and Issue: 120, P. 111228 - 111228

Published: May 13, 2024

Language: Английский

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Shaping the future of oral cancer diagnosis: advances in salivary proteomics

Expert Review of Proteomics, Journal Year: 2024, Volume and Issue: 21(4), P. 149 - 168

Published: April 2, 2024

Introduction Saliva has gained increasing attention in the quest for disease biomarkers. Because it is a biological fluid that can be collected an easy, painless, and safe way, been increasingly studied identification of oral cancer This particularly important because often diagnosed at late stages with poor prognosis.

Language: Английский

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