A multi-model study of extracellular vesicles in bowel cancer invasion and metastasis DOI Creative Commons

Sonia Guarnerio

Published: Jan. 1, 2023

Colorectal cancer (CRC) has one of the highest rates mortality worldwide. Despite progress in improving screening rates, approximately 35% CRC patients are diagnosed with stage IV (metastatic) disease which poor prognosis. In vitro 3D models have been developed provide useful platforms to explore biomolecular processes pre-clinical studies and metastasis, enabling characterisation mechanisms can then be applied improvement diagnosis, prognosis treatment. Extracellular vesicles (EVs) now known play a critical role progression. Not only do they support progression towards but as carriers bioactive cargo, also show promise potential biomarkers. this study, organotypic mimicking invasion pre-metastatic niche (PMN) lung were EVs advanced stages evaluate their prognostic markers. After validating transwell system an effective model, work establishes that induce cells stage-dependent manner, metastatic SW620 driving more aggressive phenotype than SW480 EVs. Proteomic profiling PMN showed differences protein content upon treatment list m/z signals able distinguish between EV was obtained by multivariate analysis. induced α-smooth muscle actin (α-SMA) expression fibroblasts, demonstrating activation fibroblasts model. Expression activity TG2, multifunctional enzyme involved progression, increased after model invasion. This led exploration cell-specific TG2 cells/fibroblasts co-culture, EV-mediated increase on surface non-EV mediated decrease fibroblast surface. further explored hepatic stellate cells, crucial cellular components liver metastasis. Finally, pilot biomarker study small cohort performed, using analysis cell responses plasma-derived separate according stage. The established validated provided information about complex milieu factors including EVs, thus confirming importance culture modelling research. Moreover, demonstrate key roles activities could translated for development alternative approaches may application early diagnosis CRC.

Language: Английский

Progressing nanotechnology to improve targeted cancer treatment: overcoming hurdles in its clinical implementation DOI Creative Commons
Mohammad Chehelgerdi, Matin Chehelgerdi, Omer Qutaiba B. Allela

et al.

Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)

Published: Oct. 9, 2023

Abstract The use of nanotechnology has the potential to revolutionize detection and treatment cancer. Developments in protein engineering materials science have led emergence new nanoscale targeting techniques, which offer renewed hope for cancer patients. While several nanocarriers medicinal purposes been approved human trials, only a few authorized clinical cells. In this review, we analyze some formulations discuss challenges translating findings from lab clinic. This study highlights various compounds that can be used selective tumor inherent difficulties therapy. Nanotechnology provides promising platform improving future, but further research is needed overcome current limitations translation. Graphical

Language: Английский

Citations

419
The multifaceted role of extracellular vesicles (EVs) in colorectal cancer: metastasis, immune suppression, therapy resistance, and autophagy crosstalk DOI Creative Commons
Soheil Rahmati, Aysan Moeinafshar, Nima Rezaei

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 13, 2024

Abstract Extracellular vesicles (EVs) are lipid bilayer structures released by all cells and widely distributed in biological fluids. EVs implicated diverse physiopathological processes orchestrating cell–cell communication. Colorectal cancer (CRC) is one of the most common cancers worldwide, with metastasis being leading cause mortality CRC patients. contribute significantly to advancement spread transferring their cargo, which includes lipids, proteins, RNAs, DNAs, neighboring or distant cells. Besides, they can serve as non-invasive diagnostic prognostic biomarkers for early detection be harnessed effective carriers delivering therapeutic agents. Autophagy an essential cellular process that serves remove damaged proteins organelles lysosomal degradation maintain homeostasis. EV release coordinately activated tumor share factors regulatory mechanisms. Although significance autophagy well established, exact mechanism interplay development obscure. This review focuses on examining specific functions various aspects CRC, including progression, metastasis, immune regulation, therapy resistance. Further, we overview emerging discoveries relevant crosstalk CRC.

Language: Английский

Citations

8
From Crypts to Cancer: A Holistic Perspective on Colorectal Carcinogenesis and Therapeutic Strategies DOI Open Access
Ehsan Gharib, Gilles A. Robichaud

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9463 - 9463

Published: Aug. 30, 2024

Colorectal cancer (CRC) represents a significant global health burden, with high incidence and mortality rates worldwide. Recent progress in research highlights the distinct clinical molecular characteristics of colon versus rectal cancers, underscoring tumor location's importance treatment approaches. This article provides comprehensive review our current understanding CRC epidemiology, risk factors, pathogenesis, management strategies. We also present intricate cellular architecture colonic crypts their roles intestinal homeostasis. carcinogenesis multistep processes are described, covering conventional adenoma-carcinoma sequence, alternative serrated pathways, influential Vogelstein model, which proposes sequential

Language: Английский

Citations

7
Phenotypic diversity of CTCs and tdEVs in liquid biopsies of tumour-draining veins is linked to poor prognosis in colorectal cancer DOI Creative Commons
Stefan A. Cieslik,

A. Zafra,

Christiane Driemel

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2025, Volume and Issue: 44(1)

Published: Jan. 8, 2025

Abstract Background Circulating tumour cells (CTCs) and tumour-derived extracellular vesicles (tdEVs) have great potential for monitoring therapy response early detection of relapse, facilitating personalized adjuvant therapeutic strategies. However, their low abundance in peripheral blood limits informative value. In this study, we explored the presence CTCs tdEVs collected intraoperatively from a tumour-draining vein (DV) via central venous catheter (CVC) prior to resection. Methods CellSearch analyses 395 samples 306 patients with gastrointestinal tumours 93 healthy donors were used establish validate gates automated ACCEPT software R scripts. The selected gate settings applied 227 142 colorectal cancer (CRC) two independent collectives. Phenotypic features obtained numeric analysis fluorescence signals (e.g. size, shape, intensity) calculating diversity using Shannon index (SI) clusters generated k-means algorithm after Uniform Manifold Approximation Projection (UMAP) pre-processing, standard deviation (SD). Results more abundant DV compared CVC ( p < 0.05). detected higher numbers than both compartments. Importantly, CVCs associated tumor spread, whereas DVs linked size. compartments, prognostic value overall survival (OS) surpassed that CTCs, as demonstrated by univariate, multivariate, Kaplan-Meier analyses. phenotypically distinct, being larger, eccentric, displaying stronger cytokeratin intensities 0.05) those samples. Furthermore, increased CTC tdEV phenotypes was significantly shorter survival, validating relevance SD-diversity metric. Conclusion Our study demonstrates sampling enhances prognostically relevant CRC patients, underscoring superior significance CTCs. combined phenotypic markers emerges powerful biomarker enumeration alone. These findings suggest comprehensive, may open new avenues tailoring individualized therapies patients.

Language: Английский

Citations

0
Exosomes and their distinct integrins transfer the characteristics of oxaliplatin- and 5-FU-resistant behaviors in colorectal cancer cells DOI Creative Commons

Zeynab Vakili-Ghartavol,

Hoda Deli,

Amir Shadboorestan

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: Feb. 6, 2025

Language: Английский

Citations

0
Correction: Kotelevets, L.; Chastre, E. Extracellular Vesicles in Colorectal Cancer: From Tumor Growth and Metastasis to Biomarkers and Nanomedications. Cancers 2023, 15, 1107 DOI Open Access

Larissa Kotelevets,

Eric Chastre

Cancers, Journal Year: 2025, Volume and Issue: 17(5), P. 806 - 806

Published: Feb. 26, 2025

References [...].

Language: Английский

Citations

0
M2 Macrophage‐Extracellular Vesicle‐Derived lncRNA‐NEAT1 Regulates miR‐204‐5p/RRS1‐mediated Cell Cycle to Promote the Occurrence and Development of Colorectal Cancer DOI

Guorui Ma,

Xinlin Wu,

Agudamu Agudamu

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(4)

Published: March 31, 2025

ABSTRACT Colorectal cancer (CRC) is a prevalent malignancy of the digestive system. Here, we explored role M2 macrophage‐derived extracellular vesicles (EVs) carrying long non‐coding RNA‐nuclear paraspeckle assembly transcript 1 (lncRNA‐NEAT1) in promoting CRC progression via regulation miR‐204‐5p/regulator ribosome synthesis (RRS1) axis and cell cycle dynamics. Firstly, differentiated WTHP‐1 cells into M0 macrophages transfected with sh‐NEAT1 lentivirus plasmids. EVs were isolated from administered to SW480 along miR‐204‐5p inhibitors or si‐RRS1 for 24 h. M2‐EVs‐derived lncRNA‐NEAT1 enhanced proliferation, migration, invasion, viability while reducing apoptosis. This was accompanied by increased expression RRS1, WEE1 G2 checkpoint kinase (WEE1), cyclin‐dependent (CDK1), CyclinB1, reduced levels, lower proportion G2/M phase. Knockdown reversed these effects. Mechanistically, functioned as competing endogenous RNA (ceRNA), sponging upregulate RRS1 expression. In summary, promote development modulating miR‐204‐5p/RRS1 axis, influencing These findings provide insights tumor‐promoting mechanisms CRC.

Language: Английский

Citations

0
The stimulation of exosome generation by visfatin polarizes M2 macrophages and enhances the motility of chondrosarcoma DOI

Chang‐Yu Song,

Chih‐Ying Wu, Chih‐Yang Lin

et al.

Environmental Toxicology, Journal Year: 2024, Volume and Issue: 39(7), P. 3790 - 3798

Published: March 18, 2024

Abstract Chondrosarcoma is a malignant bone tumor that arises from abnormalities in cartilaginous tissue and associated with lung metastases. Extracellular vesicles called exosomes are primarily used as mediators of intercellular signal transmission to control metastasis. Visfatin an adipokine reported enhance metastasis, but its relationship exosome generation chondrosarcoma motility remains undetermined. Our results found overexpressing visfatin augments the production cells. Visfatin‐treated educate macrophage polarization towards M2 not M1 phenotype. Interestingly, macrophages polarized by return cells facilitate cell motility. According these findings, emit more when treated visfatin. The stimulation polarizes enhances chondrosarcoma.

Language: Английский

Citations

3
The Role of Extracellular Vesicles in Colorectal Cancer DOI Creative Commons
Yujian Xia, Chaoran Yu,

Ernest Johann Helwig

et al.

Technology in Cancer Research & Treatment, Journal Year: 2023, Volume and Issue: 22

Published: Jan. 1, 2023

Extracellular vesicles (EVs) are a class of spherical that produced by active secretion cells and encapsulated phospholipid bilayers. In recent years, numerous studies have shown EVs play pivotal roles in the regulation intercellular communication between colorectal cancer (CRC) target cells, can regulate proliferation, metastasis, infiltration tumor regulating microenvironment cells. carry specific molecular substances source CRC expected to serve as new markers for detection cancers. This review highlights current state research progress potentially incorporating diagnosis treatment CRC.

Language: Английский

Citations

8
Hypoxia-regulated exosomes mediate M2 macrophage polarization and promote metastasis in chondrosarcoma DOI Creative Commons

Sheng‐Mou Hou,

Chih‐Yang Lin,

Yi‐Chin Fong

et al.

Aging, Journal Year: 2023, Volume and Issue: 15(22), P. 13163 - 13175

Published: Nov. 21, 2023

Chondrosarcoma is a primary malignant bone tumor. Traditional therapy not very effective, and it prone to metastasis in the late stage. The tumor microenvironment (TME) plays key role progression of chondrosarcoma, hypoxia one factors formation TME. However, detailed mechanism how affects chondrosarcoma still fully understood. In this study, we focused on interaction between hypoxic cells (SW1353) macrophages. Our results suggest that enhances release exosomes from cells. These hypoxia-induced promoted macrophage polarization towards M2 phenotype, characterized by expression CD163 CD206, but M1 CD86 expression. Further analysis revealed macrophages polarized expressed arginase-1 feedback promote migration. secrete more microenvironment, these hypoxia-derived induce into an ultimately promoting metastatic behavior

Language: Английский

Citations

8