Engineered exosomes: a promising vehicle in cancer therapy DOI

Farkhondeh Pooresmaeil,

Sahar Andi,

Behnam Hasannejad-Asl

et al.

Therapeutic Delivery, Journal Year: 2023, Volume and Issue: 14(12), P. 775 - 794

Published: Dec. 1, 2023

During the past few decades, researchers have attempted to discover an effective treatment for cancer. Exosomes are natural nanovesicles released by various cells and play a role in communication between cells. While exosomes high clinical potential, their inherent limitations prompted design with improved therapeutic properties. To achieve this purpose, undertaken exosome engineering modify surface properties or internal composition of exosomes. After these modifications, engineered can be used as carriers delivery chemotherapeutic agents, targeted drug development cancer vaccines. The present study provides overview exosomes, including biogenesis, biological functions, isolation techniques, methods, potential applications therapy.

Language: Английский

Modification of immune cell-derived exosomes for enhanced cancer immunotherapy: current advances and therapeutic applications DOI Creative Commons
Inseong Jung, Sanghee Shin, Moon‐Chang Baek

et al.

Experimental & Molecular Medicine, Journal Year: 2024, Volume and Issue: 56(1), P. 19 - 31

Published: Jan. 4, 2024

Abstract Cancer immunotherapy has revolutionized the approach to cancer treatment of malignant tumors by harnessing body’s immune system selectively target cells. Despite remarkable advances, there are still challenges in achieving successful clinical responses. Recent evidence suggests that cell-derived exosomes modulate generate effective antitumor responses, making them a cutting-edge therapeutic strategy. However, natural limited application due their low drug delivery efficiency and insufficient capacity. Technological advancements have allowed exosome modifications magnify intrinsic functions, load different cargoes, preferentially tumor sites. These engineered exert potent effects great potential for immunotherapy. In this review, we describe ingenious modification strategies attain desired performance. Moreover, systematically summarize tumor-controlling properties innate adaptive immunity. Collectively, review provides comprehensive intuitive guide modified exosome-based approaches, offering valuable enhance optimize

Language: Английский

Citations

46
Therapeutic potential of RNA-enriched extracellular vesicles: The next generation in RNA delivery via biogenic nanoparticles DOI

Muskan Muskan,

Pevindu Abeysinghe,

Riccardo Cecchin

et al.

Molecular Therapy, Journal Year: 2024, Volume and Issue: 32(9), P. 2939 - 2949

Published: Feb. 27, 2024

Language: Английский

Citations

38
Metabolic tagging of extracellular vesicles and development of enhanced extracellular vesicle based cancer vaccines DOI Creative Commons

Rimsha Bhatta,

Joonsu Han, Yusheng Liu

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Dec. 5, 2023

Abstract As key mediators of cellular communication, extracellular vesicles (EVs) have been actively explored for diagnostic and therapeutic applications. However, effective methods to functionalize EVs modulate the interaction between recipient cells are still lacking. Here we report a facile universal metabolic tagging technology that can install unique chemical tags (e.g., azido groups) onto EVs. The surface enable conjugation molecules via efficient click chemistry, tracking targeted modulation In context tumor EV vaccines, show toll-like receptor 9 agonists enables timely activation dendritic generation superior antitumor CD8 + T cell response. These lead 80% tumor-free survival against E.G7 lymphoma 33% B16F10 melanoma. Our study yields generate chemically tagged from parent cells, EV-cell interactions, develop potent vaccines.

Language: Английский

Citations

31
Optimizing mesenchymal stem cell extracellular vesicles for chronic wound healing: Bioengineering, standardization, and safety DOI Creative Commons
Yusuke Shimizu, Edward Hosea Ntege, Yoshikazu Inoue

et al.

Regenerative Therapy, Journal Year: 2024, Volume and Issue: 26, P. 260 - 274

Published: June 1, 2024

Chronic wounds represent a significant global burden, afflicting millions with debilitating complications. Despite standard care, impaired healing persists due to factors like persistent inflammation and tissue regeneration. Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) offer an innovative regenerative medicine approach, delivering cell-derived therapeutic cargo in engineered nanoscale delivery systems. This review examines pioneering bioengineering strategies engineer MSC-EVs into precision nanotherapeutics for chronic wounds. Emerging technologies CRISPR gene editing, microfluidic manufacturing, biomimetic systems are highlighted their potential enhance MSC-EV targeting, optimize enrichment, ensure consistent clinical-grade production. However, key hurdles remain, including batch variability, rigorous safety assessment tumorigenicity, immunogenicity, biodistribution profiling. Crucially, collaborative frameworks harmonizing regulatory science patient advocacy hold the expediting clinical translation. By overcoming these challenges, could catalyze new era of off-the-shelf therapies, restoring hope afflicted by non-healing

Language: Английский

Citations

10
Advances in exosomes utilization for clinical applications in cancer DOI
Inês A. Batista, José Carlos Machado, Sónia A. Melo

et al.

Trends in cancer, Journal Year: 2024, Volume and Issue: 10(10), P. 947 - 968

Published: Aug. 20, 2024

Language: Английский

Citations

9
Exosomes derived from natural killer cells: transforming immunotherapy for aggressive breast cancer DOI
Abdulmalik Saleh Alfawaz Altamimi, M. Arockia Babu, Muhammad Afzal

et al.

Medical Oncology, Journal Year: 2025, Volume and Issue: 42(4)

Published: March 18, 2025

Language: Английский

Citations

1
Extracellular Vesicles, Circulating Tumor Cells, and Immune Checkpoint Inhibitors: Hints and Promises DOI Creative Commons

Sara Bandini,

Paola Ulivi, Tania Rossi

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(4), P. 337 - 337

Published: Feb. 13, 2024

Immune checkpoint inhibitor (ICI) therapy has revolutionized the treatment of cancer, in particular lung while introduction predictive biomarkers from liquid biopsies emerged as a promising tool to achieve an effective and personalized response. Important progress also been made molecular characterization extracellular vesicles (EVs) circulating tumor cells (CTCs), highlighting their tremendous potential modulating microenvironment, acting on immunomodulatory pathways, setting up pre-metastatic niche. Surface antigens EVs CTCs have proved be particularly useful case immune escape mechanisms through expression immunosuppressive ligands or transport cargos that may mitigate antitumor function. On other hand, novel approaches, increase immunostimulatory molecules cargo contents can enhance response, offer premium options combinatorial clinical strategies for precision immunotherapy. In this review, we discuss recent advances identification checkpoints using CTCs, applications ICI therapy, prospective use innovative tools, considering already approved by Food Drug Administration (FDA) use, but providing good reasons intensify research both.

Language: Английский

Citations

8
Microbial‐ and host immune cell‐derived extracellular vesicles in the pathogenesis and therapy of periodontitis: A narrative review DOI Creative Commons
Jenny Jing Wang, Chun Liu,

Jason Cutler

et al.

Journal of Periodontal Research, Journal Year: 2024, Volume and Issue: unknown

Published: May 17, 2024

Abstract Periodontitis is a chronic inflammatory disease caused by dysbiotic biofilms and destructive host immune responses. Extracellular vesicles (EVs) are circulating nanoparticles released microbes cells involved in cell‐to‐cell communication, found body biofluids, such as saliva gingival crevicular fluid (GCF). EVs mainly may hold promise for diagnostic therapeutic purposes. Periodontal research has examined the potential involvement of bacterial‐ host‐cell‐derived pathogenesis, diagnosis, therapy, but data remains scarce on cell‐ or microbial‐derived EVs. In this narrative review, we first provide an overview role microbial host‐derived pathogenesis. Recent studies reveal that Porphyromonas gingivalis Aggregatibacter actinomycetemcomitans ‐derived outer membrane (OMVs) can activate cytokine release cells, while M1 macrophage contribute to bone loss. Additionally, summarised current vitro pre‐clinical utilisation cell tools context periodontal treatment. Studies indicate from M2 macrophages dendritic promote regeneration animal models. While bacterial remain underexplored preliminary suggests P. OMVs vaccine candidates. Finally, acknowledge limitations present field translating derived periodontology. It concluded cell‐derived have periodontitis pathogenesis hence be useful studying pathophysiology, treatment monitoring biomarkers.

Language: Английский

Citations

8
Innovative Strategies to Combat 5-Fluorouracil Resistance in Colorectal Cancer: The Role of Phytochemicals and Extracellular Vesicles DOI Open Access
Muttiah Barathan, Ahmad Khusairy Zulpa, Sook Luan Ng

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7470 - 7470

Published: July 8, 2024

Colorectal cancer (CRC) is a significant public health challenge, with 5-fluorouracil (5-FU) resistance being major obstacle to effective treatment. Despite advancements, 5-FU remains formidable due complex mechanisms such as alterations in drug transport, evasion of apoptosis, dysregulation cell cycle dynamics, tumor microenvironment (TME) interactions, and extracellular vesicle (EV)-mediated pathways. Traditional chemotherapy often results high toxicity, highlighting the need for alternative approaches better efficacy safety. Phytochemicals (PCs) EVs offer promising CRC therapeutic strategies. PCs, derived from natural sources, exhibit lower toxicity can target multiple pathways involved progression resistance. facilitate targeted delivery, modulate immune response, interact TME sensitize cells However, potential PCs engineered overcoming reshaping immunosuppressive underexplored. Addressing this gap crucial identifying innovative therapies enhanced reduced toxicities. This review explores multifaceted evaluates synergistic effects combining improve treatment while minimizing adverse effects. Additionally, it investigates by serving delivery vehicles modulating TME. By synthesizing current knowledge addressing research gaps, enhances academic understanding CRC, interdisciplinary involving revolutionizing therapy. Further clinical validation are essential translating these findings into improved patient outcomes.

Language: Английский

Citations

6
Extracellular Vesicles and Artificial Intelligence: Unique Weapons against Breast Cancer DOI Creative Commons
Enrica Serretiello, Annafrancesca Smimmo, Andrea Ballini

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(4), P. 1639 - 1639

Published: Feb. 18, 2024

Breast cancer (BC) caused 685,000 deaths globally in 2020, earning the title of most common type tumor among females. With a multifactorial genesis, BC is influenced by several factors such as age, genetic and epigenetic predisposition, an individual’s exposome, its classification based on morphological/histological, invasiveness, molecular futures. Extracellular vesicles (EVs) are cell-derived lipid-bilayer-delimited nanoparticles, which distinguishable size, markers expressed exosomes (40 to 150 nm), microvesicles 10,000 apoptotic bodies (100–5000 nm). Produced physiological pathological cellular contexts, EVs shuttles biological material implicated cell-to-cell communications, thus attracting significant interest diagnostic drug delivery research. We report discuss latest evidence regarding important role BC, deepening their implication tumorigenesis metastatic mechanisms. On other hand, use BC-derived prognostic biomarkers therapeutic approaches undergoing investigation. Hence, have become new weapons precision medicine; however, only with support advanced algorithms artificial intelligence (AI) can we develop wide range information. Looking ahead, it possible see application AI prognosis diagnosis different pathologies.

Language: Английский

Citations

5