The Metabolomic Footprint of Liver Fibrosis DOI Creative Commons
Diren Beyoğlu, Yury Popov, Jeffrey R. Idle

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(16), P. 1333 - 1333

Published: Aug. 11, 2024

Both experimental and clinical liver fibrosis leave a metabolic footprint that can be uncovered defined using metabolomic approaches. Metabolomics combines pattern recognition algorithms with analytical chemistry, in particular, 1H 13C nuclear magnetic resonance spectroscopy (NMR), gas chromatography–mass spectrometry (GC–MS) various liquid (LC–MS) platforms. The analysis of by each these methodologies is reviewed separately. Surprisingly, there was little general agreement between studies within three groups also groups. determined NMR (two or more hits studies) comprised elevated lactate, acetate, choline, 3-hydroxybutyrate, glucose, histidine, methionine, glutamine, phenylalanine, tyrosine citrate. For GC–MS, succinate, fumarate, malate, ascorbate, glutamate, glycine, serine and, NMR, citrate were delineated. LC–MS, only β-muricholic acid, tryptophan, acylcarnitine, p-cresol, valine phosphocholine identified. upregulated as regards tyrosine, phosphocholine. Several investigators employed traditional Chinese medicine (TCM) treatments to reverse fibrosis, commentary given on the chemical constituents may possess fibrolytic activity. It proposed molecular docking procedures TCM lead novel therapies for affecting at least one-in-twenty persons globally, which currently no pharmaceutical cure. This in-depth review summarizes relevant literature metabolomics its implications addressing problem cirrhosis sequelae.

Language: Английский

The fibrogenic niche in kidney fibrosis: components and mechanisms DOI
Li Li, Haiyan Fu, Youhua Liu

et al.

Nature Reviews Nephrology, Journal Year: 2022, Volume and Issue: 18(9), P. 545 - 557

Published: July 4, 2022

Language: Английский

Citations

219
Extracellular vesicle–matrix interactions DOI
Koushik Debnath, Kevin Las Heras, Ambar Rivera

et al.

Nature Reviews Materials, Journal Year: 2023, Volume and Issue: 8(6), P. 390 - 402

Published: March 17, 2023

Language: Английский

Citations

49
Epigenetic regulation in fibrosis progress DOI

Taixiong Xue,

Xingyu Qiu, Hongyao Liu

et al.

Pharmacological Research, Journal Year: 2021, Volume and Issue: 173, P. 105910 - 105910

Published: Sept. 22, 2021

Language: Английский

Citations

72
Pulmonary fibrosis from molecular mechanisms to therapeutic interventions: lessons from post-COVID-19 patients DOI Open Access
Chiara Giacomelli, Rebecca Piccarducci, Laura Marchetti

et al.

Biochemical Pharmacology, Journal Year: 2021, Volume and Issue: 193, P. 114812 - 114812

Published: Oct. 21, 2021

Language: Английский

Citations

62
Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Accelerate Diabetic Wound Healing via Promoting M2 Macrophage Polarization, Angiogenesis, and Collagen Deposition DOI Open Access
Liping Teng,

Maria Maqsood,

Min Zhu

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(18), P. 10421 - 10421

Published: Sept. 9, 2022

Some scholars have suggested that the clinical application of exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs-exo) might represent a novel strategy to improve diabetic wound healing. However, mechanisms underlying effects hucMSCs-exo on healing remain poorly understood. This study aimed identify mechanism in treating wounds. HucMSCs-exo were isolated (hucMSCs) and subcutaneously injected into full-thickness wounds rats. Wound closure rates histological analysis performed. The levels tumor necrosis factor-α (TNF-α), macrophage mannose receptor (MMR/CD206), platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31), vascular growth factor (VEGF) detected by immunohistochemistry. degree collagen deposition was examined using Masson's trichrome staining. Gross evaluation carried out day 0 14 post-surgery, site harvested for histology days 3, 7, post-wounding. transplantation increased In vitro, promoted proliferation vein (HUVECs) NIH-3T3 cells. vivo, reduced area inflammatory infiltration fibers. addition, tissues group had higher CD206, CD31, VEGF expressions lower TNF-α than those control 14. Our results demonstrated facilitated repair inducing anti-inflammatory macrophages promoting angiogenesis deposition.

Language: Английский

Citations

54
Extracellular Vesicles as Regulators of the Extracellular Matrix DOI Creative Commons

Neil Patel,

Anisa Ashraf, Eun Ji Chung

et al.

Bioengineering, Journal Year: 2023, Volume and Issue: 10(2), P. 136 - 136

Published: Jan. 19, 2023

Extracellular vesicles (EVs) are small membrane-bound secreted into the extracellular space by all cell types. EVs transfer their cargo which includes nucleic acids, proteins, and lipids to facilitate cell-to-cell communication. As released move from parent recipient cell, interact with matrix (ECM) acts as a physical scaffold for organization function of cells. Recent work has shown that can modulate act regulators ECM. This review will first discuss EV biogenesis mechanism transported through Additionally, we how contribute structural components aid in degradation Lastly, role influencing cells remodel ECM both pathological therapeutic contexts is examined.

Language: Английский

Citations

37
Extracellular Vesicles Are Conveyors of the NS1 Toxin during Dengue Virus and Zika Virus Infection DOI Creative Commons

Daed El Safadi,

Grégorie Lebeau,

Alisé Lagrave

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(2), P. 364 - 364

Published: Jan. 27, 2023

Extracellular vesicles (EVs), produced during viral infections, are of emerging interest in understanding infectious processes and host–pathogen interactions. EVs exosomes particular have the natural ability to transport nucleic acids, proteins, other components cellular or origin. Thus, they participate intercellular communication, immune responses, pathophysiological processes. Some viruses known hijack cell production content for their benefit. Here, we investigate whether two pathogenic flaviviruses i.e., Zika Virus (ZIKV) Dengue virus (DENV2) could an impact on features EVs. The analysis by infected cells allowed us identify that non-structural protein 1 (NS1), described as a toxin, is associated with exosomes. This observation be confirmed under conditions overexpression recombinant NS1 from each flavivirus. Using different isolation methods (i.e., exosome kit, size exclusion chromatography, Polyethylene Glycol enrichment, ELISA capture), showed was present dimer at surface excreted exosomes, this association occur extracellular compartment. finding major importance physiological context. Indeed, capacity address its implication pathophysiology diseases should explored. Furthermore, demonstrated vectorize serve useful tools vaccine development.

Language: Английский

Citations

24
Hyaluronic acid-based hydrogels: As an exosome delivery system in bone regeneration DOI Creative Commons
Huiling Deng, Jiecong Wang, Ran An

et al.

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: March 17, 2023

Exosomes are extracellular vesicles (EVs) containing various ingredients such as DNA, RNA, lipids and proteins, which play a significant role in intercellular communication. Numerous studies have demonstrated the important of exosomes bone regeneration through promoting expression osteogenic-related genes proteins mesenchymal stem cells. However, low targeting ability short circulating half-life limited their clinical application. In order to solve those problems, different delivery systems biological scaffolds been developed. Hydrogel is kind absorbable scaffold composed three-dimensional hydrophilic polymers. It not only has excellent biocompatibility superior mechanical strength but can also provide suitable nutrient environment for growth endogenous Thus, combination between hydrogels improve stability maintain activity while achieving sustained release defect sites. As an component matrix (ECM), hyaluronic acid (HA) plays critical physiological pathological processes cell differentiation, proliferation, migration, inflammation, angiogenesis, tissue regeneration, wound healing cancer. recent years, acid-based used exosome system displayed positive effects. This review mainly summarized potential mechanism HA application prospects challenges devices regeneration.

Language: Английский

Citations

24
3D Printing‐Based Hydrogel Dressings for Wound Healing DOI Creative Commons
Xuan Zhou,

Xunzhou Yu,

Tingting You

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(47)

Published: Nov. 18, 2024

Abstract Skin wounds have become an important issue that affects human health and burdens global medical care. Hydrogel materials similar to the natural extracellular matrix (ECM) are one of best candidates for ideal wound dressings most feasible choices printing inks. Distinct from hydrogels made by traditional technologies, which lack bionic mechanical properties, 3D can promptly accurately create with complex bioactive structures potential promote tissue regeneration healing. Herein, a comprehensive review multi‐functional printing‐based hydrogel healing is presented. The first summarizes techniques dressings, including photo‐curing, extrusion, inkjet, laser‐assisted printing. Then, properties design approaches series bioinks composed natural, synthetic, composite polymers described. Thereafter, application in variety environments discussed depth, hemostasis, anti‐inflammation, antibacterial, skin appendage regeneration, intelligent monitoring, machine learning‐assisted therapy. Finally, challenges prospects

Language: Английский

Citations

14
Normotensive placental extracellular vesicles provide long-term protection against hypertension and cardiovascular disease DOI Creative Commons
Yourong Feng, Sandy Lau, Qi Chen

et al.

American Journal of Obstetrics and Gynecology, Journal Year: 2024, Volume and Issue: 231(3), P. 350.e1 - 350.e24

Published: Sept. 1, 2024

Tweetable statementIn the present study, long-term protective effects of placental extracellular vesicles (EVs) from normotensive placentae in vivo were demonstrated.There was delayed onset and amelioration hypertension, as well a reduction cardiac function after EV treatment.This study also demonstrated pro-vasodilatory alteration resistance arteries attenuated renal fibrosis/small vascular thickening, further exemplifying therapeutic benefits EVs long term.Together, these results provide evidence that play an important mechanistic role longterm cardioprotective effect pregnancy on maternal CVD is reported epidemiologic literature.

Language: Английский

Citations

8