Design, printing, and engineering of regenerative biomaterials for personalized bone healthcare

Progress in Materials Science, Journal Year: 2023, Volume and Issue: 134, P. 101072 - 101072

Published: Jan. 16, 2023

Language: Английский

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Human urine-derived stem cell exosomes delivered via injectable GelMA templated hydrogel accelerate bone regeneration

Materials Today Bio, Journal Year: 2023, Volume and Issue: 19, P. 100569 - 100569

Published: Feb. 1, 2023

The key to critical bone regeneration in tissue engineering relies on an ideal bio-scaffold coated with a controlled release of growth factors. Gelatin methacrylate (GelMA) and Hyaluronic acid (HAMA) have been novel topic interest while introducing appropriate nano-hydroxyapatite (nHAP) improve its mechanical properties. And the exosomes derived from human urine-derived stem cells (human USCEXOs) also reported promote osteogenesis engineering. present study aimed design new GelMA-HAMA/nHAP composite hydrogel as drug delivery system. USCEXOs were encapsulated slow-released for better osteogenesis. characterization GelMA-based showed excellent performance vitro studies that USCEXOs/GelMA-HAMA/nHAP could marrow mesenchymal (BMSCs) angiogenesis endothelial progenitor (EPCs), respectively. Meanwhile, vivo results confirmed this significantly defect repair cranial rat model. In addition, we found can formation H-type vessels area, enhancing therapeutic effect. conclusion, our findings suggested controllable biocompatible may effectively by coupling angiogenesis.

Language: Английский

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Building Osteogenic Microenvironments with a Double-Network Composite Hydrogel for Bone Repair

Research, Journal Year: 2023, Volume and Issue: 6

Published: Jan. 1, 2023

The critical factor determining the in vivo effect of bone repair materials is microenvironment, which greatly depends on their abilities to promote vascularization and formation. However, implant are far from ideal candidates for guiding regeneration due deficient angiogenic osteogenic microenvironments. Herein, a double-network composite hydrogel combining vascular endothelial growth (VEGF)-mimetic peptide with hydroxyapatite (HA) precursor was developed build an microenvironment repair. prepared by mixing acrylated β-cyclodextrins octacalcium phosphate (OCP), HA precursor, gelatin solution, followed ultraviolet photo-crosslinking. To improve potential hydrogel, QK, VEGF-mimicking peptide, loaded β-cyclodextrins. QK-loaded promoted tube formation human umbilical vein cells upregulated expression angiogenesis-related genes, such as Flt1, Kdr, VEGF, marrow mesenchymal stem cells. Moreover, QK could recruit Furthermore, OCP be transformed into release calcium ions facilitating regeneration. integrated showed obvious osteoinductive activity. results animal experiments that enhanced skull defects rats, perfect synergistic effects vascularized In summary, improving microenvironments our shows promising prospects

Language: Английский

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Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem‐Cell Niches for In Vitro Models

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(52)

Published: April 23, 2023

Advances in bioprinting have enabled the fabrication of complex tissue constructs with high speed and resolution. However, there remains significant structural biological complexity within tissues that is unable to recapitulate. Bone, for example, has a hierarchical organization ranging from molecular whole organ level. Current techniques materials employed imposed limits on scale, speed, resolution can be achieved, rendering technique reproduce hierarchies cell-matrix interactions are observed bone. The shift toward biomimetic approaches bone engineering, where hydrogels provide biophysical biochemical cues encapsulated cells, promising approach enhancing function development vitro modeling. A major focus modeling creating dynamic microenvironmental niches support, stimulate, direct cellular processes formation remodeling. Hydrogels ideal imitating extracellular matrix since they engineered present various whilst allowing bioprinting. Here, recent advances 3D niche conducive engineering models reviewed.

Language: Английский

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Leveraging the Recent Advancements in GelMA Scaffolds for Bone Tissue Engineering: An Assessment of Challenges and Opportunities

Biomacromolecules, Journal Year: 2023, Volume and Issue: 25(4), P. 2075 - 2113

Published: July 5, 2023

The field of bone tissue engineering has seen significant advancements in recent years. Each year, over two million transplants are performed globally, and conventional treatments, such as grafts metallic implants, have their limitations. Tissue offers a new level treatment, allowing for the creation living within biomaterial framework. Recent advances biomaterials provided innovative approaches to rebuilding function after damage. Among them, gelatin methacryloyl (GelMA) hydrogel is emerging promising supporting cell proliferation regeneration, GelMA exhibited exceptional physicochemical biological properties, making it viable option clinical translation. Various methods classes additives been used application with incorporation nanofillers or other polymers enhancing its resilience functional performance. Despite results, fabrication complex structures that mimic architecture provision balanced physical properties both vasculature growth proper stiffness load bearing remain challenges. In terms utilizing osteogenic additives, priority should be on versatile components promote angiogenesis osteogenesis while reinforcing structure applications. This review focuses efforts advantages GelMA-based composite engineering, covering literature from last five

Language: Английский

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Generating human bone marrow organoids for disease modeling and drug discovery

Nature Protocols, Journal Year: 2024, Volume and Issue: 19(7), P. 2117 - 2146

Published: March 26, 2024

Language: Английский

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Nanoparticle-polymer composite scaffolds for bone tissue engineering. A review

European Polymer Journal, Journal Year: 2024, Volume and Issue: 213, P. 113093 - 113093

Published: May 7, 2024

Language: Английский

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Leveraging the predictive power of a 3D in vitro vascularization screening assay for hydrogel-based tissue-engineered periosteum allograft healing

Biomaterials Advances, Journal Year: 2025, Volume and Issue: 169, P. 214187 - 214187

Published: Jan. 16, 2025

Language: Английский

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Bone Regeneration and Oxidative Stress: An Updated Overview

Antioxidants, Journal Year: 2022, Volume and Issue: 11(2), P. 318 - 318

Published: Feb. 6, 2022

Bone tissue engineering is a complex domain that requires further investigation and benefits from data obtained over past decades. The models are increasing in complexity as they reveal new co-culturing microfluidics applications. vitro now focus on the 3D medium of osteoblasts, osteoclasts, osteocytes utilizing collagen for separation; this type research allows controlled in-depth analysis. Oxidative stress takes toll domain, being beneficial well destructive. Reactive oxygen species (ROS) molecules influence differentiation but time their presence can affect patients aid appearance diseases such osteoporosis. be limited by using antioxidants vitamin K N-acetyl cysteine (NAC). Scaffolds biocompatible coatings hydroxyapatite bioactive glass required to isolate implant, protect zone metallic, ionic exchange, enhance bone regeneration mimicking composition structure body, thus enhancing cell proliferation. materials functionalized with growth factors create better response higher chances success clinical use. This review highlights vast majority newly information regarding engineering, models, implant coatings, scaffolds, biomolecules, techniques utilized obtain them.

Language: Английский

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Self-Adhesive Hydrogel Biomimetic Periosteum to Promote Critical-Size Bone Defect Repair via Synergistic Osteogenesis and Angiogenesis

ACS Applied Materials & Interfaces, Journal Year: 2022, Volume and Issue: 14(32), P. 36395 - 36410

Published: Aug. 4, 2022

The periosteum plays an important role in the regeneration of critical-size bone defects, with functions recruiting multiple cells, accelerating vascular network reconstruction, and guiding tissue regeneration. However, these cannot be easily implemented by simply simulating via a material structure design or loading exogenous cytokines. Herein, inspired periosteal function, we propose biomimetic preparation strategy to enhance natural polymer hydrogel membranes using inorganic bioactive materials. having self-adhesive resembling extracellular matrix was prepared dopamine-modified gelatin oxidized hyaluronan (GA/HA), micro/nanobioactive glass (MNBG) further incorporated into fabricate organic/inorganic co-crosslinked membrane (GA/HA-BG). addition MNBG enhanced stability membrane, resulting sustained degradation time, biomineralization, long-term release ions. Ca2+ SiO44– ions released were shown recruit cells promote differentiation marrow stromal osteoblasts, initiating multicentric osteogenic behavior. Additionally, able continuously stimulate endogenous expression endothelial growth factor from human umbilical vein through PI3K/Akt/HIF-1α pathway, which accelerated vascularization defect area synergistically promoted repair defects. This organic–inorganic has been proved effective versatile is expected provide promising for solving clinical issues.

Language: Английский

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