Saudi Pharmaceutical Journal, Journal Year: 2025, Volume and Issue: 33(1-2)
Published: April 29, 2025
Language: Английский
International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(5), P. 1778 - 1795
Published: Jan. 1, 2024
Skin tissue, composed of epidermis, dermis, and subcutaneous is the largest organ human body.It serves as a protective barrier against pathogens physical trauma plays crucial role in maintaining homeostasis.Skin diseases, such psoriasis, dermatitis, vitiligo, are prevalent can seriously impact quality patient life.Exosomes lipid bilayer vesicles derived from multiple cells with conserved biomarkers important mediators intercellular communication.Exosomes skin cells, blood, stem main types exosomes that involved modulating microenvironment.The dysregulation exosome occurrence transmission, well alterations their cargoes, complex pathogenesis inflammatory autoimmune diseases.Therefore, promising diagnostic therapeutic targets for diseases.Importantly, exogenous exosomes, or play improving environment repairing damaged tissues by carrying various specific active substances involving variety pathways.In domain clinical practice, have garnered attention prospective agents including psoriasis vitiligo.Furthermore, investigations substantiated regenerative efficacy cell-derived repair.In this review, we mainly summarize latest studies about mechanisms applications dermatology, atopic systemic lupus erythematosus, sclerosis, diabetic wound healing, hypertrophic scar keloid, aging.This will provide novel perspective diagnosis treatment dermatosis.
Language: Английский
Citations
24
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: July 5, 2024
Abstract Diabetic wounds are characterized by incomplete healing and delayed healing, resulting in a considerable global health care burden. Exosomes lipid bilayer structures secreted nearly all cells express characteristic conserved proteins parent cell-associated proteins. harbor diverse range of biologically active macromolecules small molecules that can act as messengers between different cells, triggering functional changes recipient thus endowing the ability to cure various diseases, including diabetic wounds. accelerate wound regulating cellular function, inhibiting oxidative stress damage, suppressing inflammatory response, promoting vascular regeneration, accelerating epithelial facilitating collagen remodeling, reducing scarring. from tissues or potentially possess functions varying levels promote healing. For example, mesenchymal stem cell-derived exosomes (MSC-exos) have favorable potential field due their superior stability, permeability, biocompatibility, immunomodulatory properties. Exosomes, which derived skin components, modulate inflammation regeneration key turn promotes Therefore, this review mainly emphasizes roles mechanisms sources, represented MSCs improving A deeper understanding therapeutic will yield promising candidates perspectives for management.
Language: Английский
Citations
24
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: June 6, 2024
Abstract Adipose-derived stem cells (ADSCs) are a subset of mesenchymal (MSCs) isolated from adipose tissue. They possess remarkable properties, including multipotency, self-renewal, and easy clinical availability. ADSCs also capable promoting tissue regeneration through the secretion various cytokines, factors, extracellular vesicles (EVs). ADSC-derived EVs (ADSC-EVs) act as intercellular signaling mediators that encapsulate range biomolecules. These have been found to mediate therapeutic activities donor by proliferation migration effector cells, facilitating angiogenesis, modulating immunity, performing other specific functions in different tissues. Compared themselves, ADSC-EVs offer advantages such fewer safety concerns more convenient transportation storage for application. As result, these received significant attention cell-free agents with potential future application regenerative medicine. In this review, we focus on recent research progress regarding medical use across conditions, wound healing, chronic limb ischemia, myocardial infarction, diabetic nephropathy, fat graft survival, bone regeneration, cartilage tendinopathy tendon peripheral nerve acute lung injury, among others. We discuss underlying mechanisms responsible inducing effects. believe deciphering biological effects, associated will provide foundation developing novel approach Graphical
Language: Английский
Citations
23
Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(4)
Published: April 17, 2024
Abstract Diabetes, a group of metabolic disorders, constitutes an important global health problem. Diabetes and its complications place heavy financial strain on both patients the healthcare establishment. The lack effective treatments contributes to this pessimistic situation negative outlook. Exosomes released from mesenchymal stromal cells (MSCs) have emerged as most likely new breakthrough advancement in treating diabetes diabetes‐associated complication due capacity intercellular communication, modulating local microenvironment, regulating cellular processes. In present review, we briefly outlined properties MSCs-derived exosomes, provided thorough summary their biological functions potential uses related complications.
Language: Английский
Citations
22
International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 949 - 970
Published: Feb. 1, 2023
Purpose: Diabetic wound is a highly prevalent and refractory disease. Extensive studies have confirmed that keratinocytes macrophages play an important role in the process of healing. Additionally, exosomes are regarded as vital intercellular communication tool. This study aimed to investigate human keratinocyte-derived exosomal MALAT1 treatment diabetic by influencing biological function macrophages. Methods: We mainly assessed on changes macrophages, expression keratinocyte-exosomes analyzed quantitative real-time polymerase chain reaction (RT-qPCR). The downstream interaction between RNAs or proteins was mechanistic experiments. Besides, we evaluated effects healing vivo verify vitro results. Results: demonstrated enhanced functions high glucose-injured including phagocytosis, converting pro-healing phenotype reducing apoptosis. Mechanistically, accelerated MFGE8 competitively binding miR-1914-3p, thereby affecting signal axis TGFB1/SMAD3, finally promoting wounds. Human might promote collagen deposition, ECM remodeling, MFGE8, VEGF, CD31 but reduce TGFB SMAD3 model mice wounds, which restored its function. Conclusion: current revealed would suppress miR-1914-3p activate eventually enhancing macrophage It proposed capacity serve new method for clinical wound. Graphical Abstract: Keywords: exosomes, keratinocytes, MALAT1,
Language: Английский
Citations
25
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5703 - 5703
Published: March 16, 2023
Androgenic alopecia (AGA) is the most common type of hair loss, where local high concentrations dihydrotestosterone (DHT) in scalp cause progressive shrinkage follicles, eventually contributing to loss. Due limitations existing methods treat AGA, use multi-origin mesenchymal stromal cell-derived exosomes has been proposed. However, functions and mechanisms action secreted by adipose cells (ADSCs-Exos) AGA are still unclear. Using Cell Counting Kit-8 (CCK8) analysis, immunofluorescence staining, scratch assays, Western blotting, it was found that ADSC-Exos contributed proliferation, migration, differentiation dermal papilla (DPCs) up-regulated expression cyclin, β-catenin, versican, BMP2. also mitigated inhibitory effects DHT on DPCs down-regulated transforming growth factor-beta1 (TGF-β1) its downstream genes. Moreover, high-throughput miRNA sequencing bioinformatics analysis identified 225 genes were co-expressed ADSC-Exos; these, miR-122-5p highly enriched luciferase assays target SMAD3. carrying antagonized inhibition β-catenin versican vivo vitro, restored bulb size thickness, promoted normal follicles. So, enhanced regeneration follicles through TGF-β/SMAD3 axis. These results suggest a novel treatment option for AGA.
Language: Английский
Citations
23
Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 588, P. 112213 - 112213
Published: March 29, 2024
Language: Английский
Citations
9
BMC Endocrine Disorders, Journal Year: 2025, Volume and Issue: 25(1)
Published: Jan. 2, 2025
Hydroxychloroquine (HCQ) is frequently utilized in rheumatic immune disorders and has been discovered to exert hypoglycemic effects some obese women with polycystic ovary syndrome(PCOS), however, the precise efficacy mechanism of action remain ambiguous. To examine impact HCQ on glucose lipid metabolism as well sex hormone levels PCOS. Fifty PCOS were randomly allocated into two groups: group (n = 25) metformin (MET) 25). The received a daily dose 200 mg hydroxychloroquine, while MET 1000 metformin. Body fat parameters, levels, evaluated. Additionally, incidence pregnancy within six months following treatment was also assessed. Network pharmacology employed analyze potential molecular mechanism. Patients 20) 23) ultimately included for analysis. Following treatment, both groups exhibited significant improvements body distribution status, demonstrating notable advantage over increasing insulin sensitivity index (ISI)(HCQ:1.87 ± 0.21,MET:1.75 0.29). Serum [Serum total cholesterol(TC, mmol/L)(HCQ:4.51 0.87,MET:5.05 0.65), triglyceride(TG, mmol/L)(HCQ:1.36 0.51,MET:1.67 0.72), low-density lipoprotein (LDL, mmol/L)(HCQ:2.66 0.98,MET:0.47 1.42),decreased post-treatment, displaying clear advantages compared group. improvement not pronounced either group, although there an overall downward trend. benefits management include distribution, correction hormonal disorders. study officially registered clinical trial April 17, 2022, registration number ChiCTR2200058816. https://www.chictr.org.cn/showproj.html?proj=160099 .
Language: Английский
Citations
1
Burns & Trauma, Journal Year: 2025, Volume and Issue: 13
Published: Jan. 1, 2025
Poor wound healing is a refractory process that places an enormous medical and financial burden on diabetic patients. Exosomes have recently been recognized as crucial players in the of lesions. They excellent stability, homing effects, biocompatibility, reduced immunogenicity novel cell-free therapies. In addition to transporting cargos target cells enhance intercellular communication, exosomes are beneficial nearly every phase healing. participate modulating inflammatory response, accelerating proliferation reepithelization, increasing angiogenesis, regulating extracellular matrix remodeling. Accumulating evidence indicates hydrogels or dressings conjunction with can prolong duration exosome residency wounds. This review provides overview mechanisms, delivery, clinical application, engineering, existing challenges use repair. We also propose future directions for biomaterials incorporating exosomes: 2D 3D scaffolds, loaded healing-promoting gases, intelligent biomaterials, prospect systematic application exosomes. These findings may might shed light treatments enlighten some studies improve quality life among diabetes
Language: Английский
Citations
1
Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14
Published: Feb. 28, 2023
Diabetic foot is one of the most common complications diabetes, requiring repeated surgical interventions and leading to amputation. In absence effective drugs, new treatments need be explored. Previous studies have found that stem cell transplantation can promote healing chronic diabetic wounds. However, safety issues limited clinical application this technique. Recently, performance mesenchymal cells after has been increasingly attributed their production exocrine functional derivatives such as extracellular vesicles (EVs), cytokines, cell-conditioned media. EVs contain a variety cellular molecules, including RNA, DNA proteins, which facilitate exchange information between cells. several advantages over parental cells, high profile, no immune response, fewer ethical concerns, reduced likelihood embolism formation carcinogenesis. paper, we summarize current knowledge cell-derived in accelerating wound healing, well potential clinic applications.
Language: Английский
Citations
22