Survey of Ophthalmology, Journal Year: 2024, Volume and Issue: 69(6), P. 893 - 904
Published: June 26, 2024
Language: Английский
JCI Insight, Journal Year: 2025, Volume and Issue: 10(5)
Published: March 9, 2025
Glioblastoma (GBM) is the most lethal brain cancer, with GBM stem cells (GSCs) driving therapeutic resistance and recurrence. Targeting GSCs offers a promising strategy for preventing tumor relapse improving outcomes. We identify SUV39H1, histone-3, lysine-9 methyltransferase, as critical GSC maintenance progression. SUV39H1 upregulated in compared normal tissues, single-cell RNA-seq showing its expression predominantly due to super-enhancer-mediated activation. Knockdown of impaired their proliferation stemness. Whole-cell analysis revealed that regulates G2/M cell cycle progression, maintenance, death pathways GSCs. By integrating data ATAC-seq data, we further demonstrated knockdown altered chromatin accessibility key genes associated these pathways. Chaetocin, an inhibitor, mimics effects knockdown, reducing stemness sensitizing temozolomide, standard chemotherapy. In patient-derived xenograft model, targeting inhibits GSC-driven growth. Clinically, high correlates poor glioma prognosis, supporting relevance target. This study identifies crucial regulator target improve treatment patient
Language: Английский
Citations
4
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(9), P. 8227 - 8227
Published: May 4, 2023
The aim of this study is to evaluate molecules involved in oxidative stress (OS), inflammation, angiogenesis, and apoptosis, discern which these are more likely be implicated proliferative diabetic retinopathy (PDR) macular edema (DME) by investigating the correlation between them plasma (PLS) vitreous body (VIT), as well examining data obtained from ophthalmological examinations. Type 2 (T2DM) patients with PDR/DME (PDRG/DMEG; n = 112) non-DM subjects surrogate controls (SCG 48) were selected according inclusion/exclusion criteria programming for vitrectomy, either due having or hole (MH)/epiretinal membrane (ERM)/rhegmatogenous retinal detachment. Blood samples collected processed determine glycemic profile, total cholesterol, C reactive protein, malondialdehyde (MDA), 4-hydroxynonenal (4HNE), superoxide dismutase (SOD), catalase (CAT) levels antioxidant capacity (TAC). In addition, interleukin 6 (IL6), vascular endothelial growth factor (VEGF), caspase 3 (CAS3) assayed. VITs measure expression all abovementioned molecules. Statistical analyses conducted using R Core Team (2022) program, including group comparisons analyses. Compared SCG, our findings support presence OS, apoptosis PLS VIT T2DM. PDRG, there was a decrease load (p < 0.001) an increase pro-angiogenic 0.001), but pro-oxidants decline antioxidants intravitreally. DMEG, pro-inflammatory augmented diminished increased decreased Furthermore, we found positive PLS-CAT VIT-SOD (rho 0.5; p 0.01) negative PSD-4HNE VIT-TAC DMEG. Integrative imaging variables showed central subfield foveal thickness (CSFT) 0.01), CSFT VIT-4HNE 0.4; PDRG. displayed VIT-CAT 0.01). Exploring relationship potential biomarkers may help detecting early molecular changes PDR/DME, can used identify at high risk progression, monitor therapeutic outcomes retina.
Language: Английский
Citations
35
Antioxidants, Journal Year: 2024, Volume and Issue: 13(5), P. 594 - 594
Published: May 12, 2024
Diabetic retinopathy (DR) represents a severe complication of diabetes mellitus, characterized by irreversible visual impairment resulting from microvascular abnormalities. Since the global prevalence continues to escalate, DR has emerged as prominent area research interest. The development and progression encompass complex interplay pathological physiological mechanisms, such high glucose-induced oxidative stress, immune responses, vascular endothelial dysfunction, well damage retinal neurons. Recent years have unveiled involvement genomic epigenetic factors in formation mechanisms. At present, extensive explores potential biomarkers cytokines, molecular cell therapies, antioxidant interventions, gene therapy for treatment. Notably, certain drugs, anti-VEGF agents, antioxidants, inhibitors inflammatory protein kinase C (PKC)-β inhibitors, demonstrated promising outcomes clinical trials. Within this context, review article aims introduce recent on highlight current progress field, with particular focus emerging experimental treatment strategies targeting redox signaling pathways.
Language: Английский
Citations
14
Travel Medicine and Infectious Disease, Journal Year: 2024, Volume and Issue: 58, P. 102689 - 102689
Published: Feb. 1, 2024
High altitude retinopathy (HAR) is a common ocular disorder that occurs on ascent to high altitude. There are many clinical symptoms, retinal vascular dilatation, edema and hemorrhage common. These usually do not or slightly affect vision; rarely, severe cases develop serious permanent vision loss. At present, the research progress of HAR mainly focuses hemodynamic changes, blood-retinal barrier damage, oxidative stress inflammatory response. Although related studies limited, it shows still belongs hypoxia, hypobaric hypoxia plays an aggravating role in promoting development disease. Various have demonstrated correlation with acute mountain sickness (AMS) high-altitude cerebral (HACE), so deeper understanding important. The slow rates key preventing any sickness. Research traditional chinese medicine (TCM) western has been gradually carried out. Further exploration pathogenesis prevention strategies will provide better guidance for doctors travelers.
Language: Английский
Citations
12
Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 367, P. 708 - 736
Published: Feb. 10, 2024
Language: Английский
Citations
12
Cureus, Journal Year: 2024, Volume and Issue: unknown
Published: March 21, 2024
Diabetic retinopathy, nephropathy, and neuropathy are significant microvascular complications of diabetes mellitus, contributing to substantial morbidity mortality worldwide. This comprehensive review examines the clinical relationship between these complications, focusing on shared pathophysiological mechanisms, bidirectional relationships, implications for patient management. The highlights importance understanding interconnected nature diabetic adopting a holistic approach care. Insights gleaned from this underscore necessity early detection, timely intervention, integrated care models involving collaboration among healthcare professionals. Furthermore, emphasizes need continued research elucidate underlying identify novel therapeutic targets, assess efficacy strategies in improving outcomes. By fostering interdisciplinary knowledge exchange, future endeavors hold potential advance our management ultimately enhancing quality life.
Language: Английский
Citations
12
Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11
Published: March 22, 2024
In the field of eye health, profound impact exercise and physical activity on various ocular diseases has become a focal point attention. This review summarizes elucidates positive effects activities common diseases, including dry disease (DED), cataracts, myopia, glaucoma, diabetic retinopathy (DR), age-related macular degeneration (AMD). It also catalogues offers recommendations based varying impacts that different types intensities may have specific conditions. Beyond correlations, this compiles potential mechanisms through which beneficially affect health. From mitigating oxidative stress inflammatory responses, reducing intraocular pressure, enhancing mitochondrial function, to promoting blood circulation release protective factors, complex biological triggered by reveal their substantial in preventing even assisting treatment diseases. aims not only foster awareness appreciation for how can improve health but serve as catalyst further exploration into key targets Such inquiries are crucial advancing innovative strategies thereby holding significant implications development new therapeutic approaches.
Language: Английский
Citations
11
Experimental Cell Research, Journal Year: 2024, Volume and Issue: 441(2), P. 114170 - 114170
Published: July 15, 2024
Diabetic retinopathy (DR) is a major cause of vision loss and blindness in adults. Cellular senescence was involved the pathogenesis early-stage DR positively correlated with progression. Thus, our study aimed at exploring effect potential mechanism Mesenchymal stem cells-derived exosomes (MSCs-EXOs) on Retinal Pigment Epithelial (RPE) cells an early stage vivo vitro. ARPE-19 were incubated high glucose (HG) medium mixed MSCs-EXOs to observe changes cell viability. Senescence-associated β-galactosidase (SA-β-gal) staining, Western blot qRT-PCR used assess expression senescence-related genes antioxidant mediators. Quantitative Real-Time polymerase chain reaction (qRT-PCR), Optical coherence tomography (OCT) Hematoxylin eosin (HE) staining Electroretinogram (ERG) respectively verify cellular senescence, structure function retina. Our findings demonstrated that inhibited HG-induced cells. Furthermore, reduced apoptosis oxidative stress levels while promoting proliferation. Mechanistically, HG suppressed PI3K/AKT phosphorylation as well nuclear factor erythroid 2-related 2 (Nrf2) along its downstream target gene However, reversed these by alleviating enhancing activity. In line results vitro, significantly ameliorated hyperglycemia-induced mice downregulating mRNA P53, P21, P16, SASP. Additionally, improved functional structural integrity retina mice. revealed protective offering new insights for treatment DR.
Language: Английский
Citations
11
Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(5), P. 1327 - 1327
Published: Feb. 26, 2024
Diabetic macular edema (DME) is a common complication of diabetes mellitus and leading cause visual impairment worldwide. It defined as the diabetes-related accumulation fluid, proteins, lipids, with retinal thickening, within area. DME affects significant proportion individuals diabetes, prevalence increasing disease duration severity. estimated that approximately 25–30% diabetic patients will develop during their lifetime. Poor glycemic control, hypertension, hyperlipidemia, duration, genetic predisposition are recognized risk factors for development progression DME. Although exact pathophysiology still not completely understood, it has been demonstrated chronic hyperglycemia triggers cascade biochemical processes, including increased oxidative stress, inflammation, activation vascular endothelial growth factor (VEGF), cellular dysfunction, apoptosis, breakdown blood-retinal barriers fluid Early diagnosis appropriate management crucial improving outcomes. control systemic remains most important strategy in treatment, intravitreal pharmacotherapy anti-VEGF molecules or steroids currently considered first-line approach patients, whereas laser photocoagulation pars plana vitrectomy may be useful selected cases. Available steroids, triamcinolone acetonide injections dexamethasone fluocinolone implants, exert therapeutic effect by reducing inhibiting VEGF expression, stabilizing barrier thus permeability. They have to effective outcomes but associated high intraocular pressure elevation cataract development, so use requires an accurate patient selection. This manuscript aims provide comprehensive overview pathology, epidemiology, factors, physiopathology, clinical features, treatment mechanisms actions, options, prognosis, ongoing studies related DME, particular consideration therapy.
Language: Английский
Citations
10
Life, Journal Year: 2025, Volume and Issue: 15(2), P. 263 - 263
Published: Feb. 9, 2025
Diabetic retinopathy (DR), a leading cause of vision impairment worldwide, is characterized by progressive damage to the retina due prolonged hyperglycemia. Despite advances in treatment, current interventions largely target late-stage vascular complications, leaving underlying neurodegenerative processes insufficiently addressed. This article explores crucial role neuronal survival, axonal growth, and synaptic plasticity neuroprotective potential Brain-Derived Neurotrophic Factor (BDNF) as therapeutic strategy for enhancing retinal resilience DR. Furthermore, it discusses innovative delivery methods BDNF, such gene therapy nanocarriers, which may overcome challenges achieving sustained targeted levels retina, focusing on early intervention preserve function prevent loss.
Language: Английский
Citations
2