Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(20), P. 6184 - 6184
Published: Oct. 17, 2024
Reproductive disorders are common events in modern reproductive medicine, occurring both spontaneous and assisted pregnancies. Studies on the molecular mechanisms of implantation thin endometria, including study gene transcriptional activities, have shed light identification potential biological markers endometrial receptivity.
Language: Английский
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2834 - 2834
Published: Feb. 29, 2024
Evaluation of the optimal number embryos, their quality, and precise timing for transfer are critical determinants in reproductive success, although still remaining one main challenges assisted reproduction technologies (ART). Indeed, success vitro fertilization (IVF) treatments relies on a multitude events factors involving both endometrium embryo. Despite concerted efforts fronts, overall rates IVF techniques continue to range between 25% 30%. The role implantation has been recently recognized, leading hypothesis that “soil” “seed” play central successful pregnancy. In this respect, identification molecular signature endometrial receptivity together with selection best embryo become crucial ART. Currently, have made develop accurate, predictive, personalized tests identify window quality However, value these is debated, as conflicting results reported literature. purpose review summarize critically report available criteria optimize better understand current limitations potential areas improvement.
Language: Английский
Citations
6
Human Reproduction, Journal Year: 2023, Volume and Issue: 38(7), P. 1239 - 1244
Published: April 29, 2023
Abstract After more than a decade of increasingly widespread clinical use, personalized embryo transfer guided by endometrial receptivity analysis (ERA) remains controversial and unproven. One key element missing from the historical literature is recognition that potential benefits are entirely dependent on accuracy predictive value ERA test. Results first comprehensive trial, designed in way allowed independent evaluation both test upon which it based, were recently published. However, authors failed to conduct an appropriate or recognize significance their results. Here, we present simple reanalysis data this otherwise excellent randomized controlled demonstrating for time was unable identify window implantation as purported that, result, based actually reduced rather increased birth rates. Based these results lack any contradictory evidence, our opinion all use ERA-guided should be discontinued immediately, outside experimental setting with informed consent participating patients.
Language: Английский
Citations
13
Diagnostics, Journal Year: 2025, Volume and Issue: 15(7), P. 868 - 868
Published: March 28, 2025
Background: Vascular Endothelial Growth Factor (VEGF) is a key regulator in angiogenesis and contributes to successful implantation. The current study has the following objective: perform genotyping haplotyping analysis confirm whether VEGF-936C/T, VEGF-634C/G, VEGF-2578C/A gene polymorphisms are associated with susceptibility for recurrent implantation failure (RIF) Romanian females at reproductive age. Materials Methods: In total, 41 infertile women experiencing 44 minor infertility were genotyped VEGF using PCR-RFLP analysis. Results: VEGF-936C/T polymorphism dominant model, (C/T+T/T), represents an increased risk factor failure, odds being 2.70 (95% CI: [1.04, 7.00]). Also, of RIF under codominant (adjusted-OR = 5.28, 95% [1.42, 19.65]) recessive models 5.15, [1.55, 17.09]). Patients carrying VEGF-T936 allele or VEGF-C2578 had 2.25-fold 2.36-fold [1.05, 4.81], p 0.034) [1.27, 4.39], 0.006), respectively. results haplotype-based regression reveal that patient carriers VEGF-936/-634/-2578 T-C-A haplotype 12.39 RIF. VEGF-936/-2578 T-A 9.56-fold (p 0.0113) after adjusting Conclusions: We found significant association between this cohort women. Haplotype suggested role haplotypes as factors
Language: Английский
Citations
0
Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11
Published: May 15, 2024
Introduction Embryo implantation requires synchronous communication between the embryo and maternal endometrium. Inadequate endometrial receptivity is one of principal causes for failure [especially repeated (RIF)] when biopsied good-quality euploid embryos are transferred. An RNA-seq-based test (rsERT) was previously established to precisely guide successful implantation. In this study, we aimed evaluate effect personalized transfer (pET) via rsERT on clinical outcomes in patients with RIF. Methods A total 155 RIF were included present retrospective study divided into two groups: 60 who underwent pET (Group rsERT) 95 standard frozen (FET) without FET). Reproductive compared rsERT-guided FET. Results Forty percent (24/60) receptive, remaining 60% (36/60) non-receptive. The positive human chorionic gonadotropin (β-hCG) rate (56.3% vs. 30.5%, P = 0.003) pregnancy (43.8% 24.2%, 0.017) significantly higher Group than FET group patients. Additionally, also showed a (32.1% 22.1%, 0.104) live birth (35.4% 21.1%, 0.064) patients, although significance. For subpopulation analysis, β-hCG rate, receptive not statistically significant different from those non-receptive Conclusions can improve indicating potential pET.
Language: Английский
Citations
2
Cureus, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 26, 2024
Embryo transfer is a pivotal procedure in assisted reproductive technologies (ART). Yet, the success of this process hinges on multiple factors, with endometrial receptivity playing critical role determining likelihood successful implantation. The array (ERA) an advanced diagnostic tool designed to personalize embryo timing by assessing endometrium's receptivity. This review comprehensively examines ERA, exploring its biological foundation, technological development, and clinical applications. ERA's ability analyze expression genes associated offers tailored approach identifying optimal window implantation (WOI), particularly benefiting patients recurrent failure (RIF) or repeated unsuccessful vitro fertilization (IVF) cycles. Clinical outcomes from ERA-guided transfers indicate improvements rates overall pregnancy success, although challenges such as result variability cost-effectiveness persist. also discusses latest advancements ERA technology, including integrating genomic transcriptomic analyses, non-invasive techniques, using artificial intelligence (AI). Controversies regarding widespread application necessity all IVF cases are critically examined. By summarizing current state transfer, aims inform clinicians, researchers, about potential enhance ART highlight areas for future research innovation.
Language: Английский
Citations
1
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9746 - 9746
Published: Sept. 9, 2024
According to the current data, endometrium acts as a “sensor” of embryo quality, which promotes implantation euploid embryos and prevents and/or subsequent development genetically abnormal embryos. The present review addresses nature “sensory function” highlights necessity for assessing its functional status. first section examines evolutionary origin “sensory” ability consequence spontaneous decidualization that occurred in placental animals. second details mechanisms implementing this function at cellular level. In particular, recent findings appearance different cell subpopulations during are described, their role is discussed. pathological consequences an imbalance among these also Finally, third summarizes information on currently available clinical tools assess endometrial advantages disadvantages approaches emphasized, possible options developing more advanced technologies proposed.
Language: Английский
Citations
1
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3670 - 3670
Published: March 25, 2024
Despite serum progesterone being a widely accepted method for luteal phase support during embryo transfer cycles, debates persist regarding the optimal strategy guiding clinical decisions on dosages to maximize reproductive outcomes. This retrospective study explored utility of microRNA (miRNA) biomarkers in personalized dosage adjustments frozen (FET) cycles 22 vitro fertilization (IVF) patients undergoing hormone replacement therapy. Utilizing MIRA, an miRNA-based endometrial receptivity test, we analyzed patients’ miRNA expression profiles before and after determine suitable assess status. receiving identical dosages, variations were observed initial cycle, all presented displaced window implantation. Following based their profiles, 91% successfully transitioned endometrium towards receptive stages. However, two continued exhibit persistent despite adjustments. Given evident variation status levels among individuals, analyzing may address challenge inter-personal levels, deliver more facilitate IVF.
Language: Английский
Citations
1
Published: Aug. 13, 2024
Nowadays recurrent implantation failure (RIF) after invitro fertilization is common problem that reproductive specialists usually faced. The phenomenon of thin endometrium in RIF patients has yet not completely understood and properly treated. This study aimed to reveal significantly dysregulated selected genes expression between with fertile women. Endometrial samples were collected the window days (LH+7 – LH+10) natural menstrual cycle from (n=20) women (n=14). Ten chosen as target regarding their possible relations process. gene levels showed differences compared samples. significant downregulation was observed for CXCL1 (p=0.005) C4BPA (p=0.03) genes. There no statistically difference groups group 8 genes: CXCL8, HPRT1, MMP10, INFG, VEGFB, HAND2, IL15 TNC (p>0.05). use a combination two markers (C4BPA + CXCL1) allows good discrimination (AUC 0.806).
Language: Английский
Citations
1
Published: Aug. 14, 2024
Reproductive disorders are a common event in modern reproductive medicine, occurring both spontaneous and assisted pregnancies. Studies of the molecular mechanisms implantation thin endometrium, including study gene transcriptional activity, have shed light on identification potential biological markers endometrial receptivity. Background/Objectives: to reveal significantly dysregulated selected genes expression between RIF RPL patients with endometrium. Methods: Endometrial samples were collected window days (LH+7 – LH+10) natural menstrual cycle from (n=20) (n=19). Ten chosen as target regarding their possible relations process. Total RNA was purified, reverse-transcribed; quantified by RT-PCR. Results: The IL-15, INFG HPRT1 decreased endometrium compared (log2 fold change=0.92, p=0.023 for log2 change=1.24, p=0.046 INFG, change=0.579, HPRT1). There not significant differences CXCL8, CXCL1, MMP10, C4BPA, TNC, VEGFB HAND2 groups. Conclusions: found women RPL. This has practical significance clinicians differentiated prescription immunomodulatory therapy undergoing ART programs.
Language: Английский
Citations
1
Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14
Published: July 31, 2023
Successful embryo implantation, is the initiating step of pregnancy, relies on not only high quality but also synergistic development a healthy endometrium. Characterization and identification biomarkers for receptive endometrium an effective method increasing probability successful implantation. Endometrial tissues from 22 women with history recurrent implantation failure (RIF) 19 fertile controls were collected using biopsy catheters 7-9 days after peak luteinizing hormone. Differentially expressed proteins (DEPs) identified in six patients RIF isobaric tag relative absolute quantitation (iTRAQ)-based proteomics analysis. Two hundred sixty-three DEPs, including multiple bioactivities, such as protein translation, mitochondrial function, oxidoreductase activity, fatty acid amino metabolism, iTRAQ. Four potential named tubulin polymerization-promoting family member 3 TPPP3, S100 Calcium Binding Protein A13 (S100A13), 17b-hydroxysteroid dehydrogenase 2 (HSD17B2), alpha-2-glycoprotein 1, zinc binding (AZGP1) further verified ProteinSimple Wes immunohistochemical staining all included samples (n=22 n=19 controls). Of four proteins, levels TPPP3 HSD17B2 significantly downregulated RIF. Poor endometrial receptivity considered main reason decrease pregnancy success rates suffering iTRAQ techniques based isotope markers can identify quantify low abundance proteomics, may be suitable identifying differentially This study provides novel evidence that targets diagnosis treatment non-receptive
Language: Английский
Citations
3