Recycling Food Waste and Saving Water: Optimization of the Fermentation Processes from Cheese Whey Permeate to Yeast Oil

Fermentation, Journal Year: 2022, Volume and Issue: 8(7), P. 341 - 341

Published: July 19, 2022

With the aim of developing bioprocesses for waste valorization and a reduced water footprint, we optimized two-step fermentation process that employs oleaginous yeast Cutaneotrichosporon oleaginosus production oil from liquid cheese whey permeate. For first step, addition urea as cost-effective nitrogen source allowed an increase in biomass production. In second syrup candied fruit processing, another food supplied carbon feeding, triggered lipid accumulation. Consequently, lipids were produced at final concentration productivity 38 g/L 0.57 g/L/h respectively, which are among highest reported values. Through this strategy, based on wastes (WP mango syrup) by recovering not only nutritional compounds but also necessary growth production, addressed one main goals circular economy. addition, set up accurate fast-flow cytometer method to quantify content, avoiding extraction step use solvents. This can represent analytical improvement screening different strains monitoring single-cell level.

Language: Английский

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Isolation, molecular identification of lipid-producing Rhodotorula diobovata: optimization of lipid accumulation for biodiesel production

Journal of Genetic Engineering and Biotechnology, Journal Year: 2022, Volume and Issue: 20(1), P. 32 - 32

Published: Feb. 21, 2022

The increased demand for oil and fats to satisfy the ever-increasing human needs has enhanced research in this field. Single-cell oils or microbial lipids produced by oleaginous microorganisms are being utilized as an alternative traditional sources. Oleaginous yeasts can accumulate above 20% of their biomass when they grown under controlled conditions. In present study, sixty-five were isolated from different Using Sudan Black B staining technique, five yeast isolates selected. Under nitrogen-limited cultivation conditions, Co1 isolate was best lipid accumulation potential 39.79%. Isolate (Co1) characterized morphologically identified using ribosomal DNA internal transcribed spacers regions (rDNA-ITS) genomic DNA. sequence alignment revealed a 99.2% similarity with Rhodotorula diobovata. optimized diobovata accumulated up 45.85% on dry basis. R. diobovata, raw materials, 46.68% sugar beet molasses medium, had high degree monounsaturated fatty acids which gives biodiesel better quality. data suggest that potent yeast, together use cheap feedstock materials such molasses, be considered promising production.

Language: Английский

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Optimization of volatile fatty acid production by sugarcane vinasse dark fermentation using a response surface methodology. Links between performance and microbial community composition

Sustainable Energy Technologies and Assessments, Journal Year: 2022, Volume and Issue: 53, P. 102764 - 102764

Published: Sept. 29, 2022

Language: Английский

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Cutaneotrichosporon oleaginosus: A Versatile Whole-Cell Biocatalyst for the Production of Single-Cell Oil from Agro-Industrial Wastes

Catalysts, Journal Year: 2021, Volume and Issue: 11(11), P. 1291 - 1291

Published: Oct. 27, 2021

Cutaneotrichosporon oleaginosus is an oleaginous yeast with several favourable qualities: It fast growing, accumulates high amounts of lipids and has a very broad substrate spectrum. Its resistance to hydrolysis by-products makes it promising biocatalyst for custom tailored microbial oils. C. can accumulate up 60 wt.% its biomass as lipids. This species able grow by using compounds substrate, such acetic acid, biodiesel-derived glycerol, N-acetylglucosamine, lignocellulosic hydrolysates, wastepaper other agro-industrial wastes. review focused on state-of-the-art innovative sustainable biorefinery schemes involving this second- third-generation biomasses. Moreover, offers comprehensive updated summary process strategies, pretreatments fermentation conditions enhancing lipid production whole-cell biocatalyst. Finally, overview the main industrial applications single-cell oil reported together future perspectives.

Language: Английский

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Enhanced production of biodiesel by Rhodosporidium toruloides using waste office paper hydrolysate as feedstock: Optimization and characterization

Fuel, Journal Year: 2022, Volume and Issue: 327, P. 125174 - 125174

Published: July 11, 2022

Language: Английский

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