Gels,
Journal Year:
2022,
Volume and Issue:
9(1), P. 14 - 14
Published: Dec. 26, 2022
Protein-based
hydrogels
have
attracted
growing
attention
for
pharmaceutical
and
biomedical
applications.
Ovalbumin
(OVA),
the
hen
egg
white
albumin,
possessing
good
foaming
gelling
properties
being
widely
used
in
food
industry,
has
recently
been
indicated
as
a
potential
vehicle.
In
this
study,
binding
release
of
pure
OVA
were
investigated
by
electron
paramagnetic
resonance
(EPR)
spin
labeling.
The
comparative
analysis
between
serum
albumin
(SA)
revealed
same
kinetics
hydrophilic
3-carbamoyl-proxyl
3-carboxy-proxyl,
suggesting
diffusion-dominated
small
probes
from
both
hydrogel
types.
results
obtained
with
amphiphilic
16-doxylstearate
(16-DS)
indicate
that
OVA,
unlike
SAs,
does
not
possess
specific
fatty
acid
site.
However,
able
to
accommodate
two-fold
excess
16-DS,
resulting
protein
thermally
induced
conformational
changes,
confirmed
Raman
spectroscopy.
Similarly,
hydrophobic
modified
paullone
ligand
HL,
which
was
initially
free
solution,
bound
hydrogel.
found
retain
significant
amount
16-DS
HL
after
7-day
dialysis
physiological
saline.
observed
facilitated
amphiphilic/hydrophobic
molecules
compared
their
sustained
release,
demonstrate
applicability
pharmaceutics.
ACS Omega,
Journal Year:
2023,
Volume and Issue:
8(4), P. 3551 - 3570
Published: Jan. 20, 2023
Owing
to
their
properties
such
as
biocompatibility,
tunable
mechanical
properties,
permeability
toward
oxygen,
nutrients,
and
the
ability
hold
a
significant
amount
of
water,
hydrogels
have
wide
applications
in
biomedical
research.
They
been
engaged
drug
delivery
systems,
3D
cell
culture,
imaging,
extracellular
matrix
(ECM)
mimetics.
Injectable
represent
major
subset
possessing
advantages
site-specific
conformation
with
minimal
invasive
techniques.
It
preserves
inherent
drug/biomolecules
is
devoid
any
side
effects
associated
surgery.
Various
polymeric
materials
utilized
developing
injectable
are
limitations
toxicity,
immunogenicity,
tedious
manufacturing
processes,
lack
easy
synthetic
tunability.
Peptides
an
important
class
biomaterials
that
interesting
stimuli
responsiveness,
shear
thinning,
self-healing,
biosignaling.
immunogenicity
toxicity.
Therefore,
numerous
peptide-based
explored
past,
few
them
reached
market.
In
recent
years,
minimalistic
dipeptides
shown
form
stable
through
cooperative
noncovalent
interactions.
addition
lengthy
hydrogels,
unique
low
production
cost,
high
accessibility,
higher
stability.
Given
instances
expanding
significance
peptide
research
emerging
trend
dipeptide-based
timely
review
on
shall
highlight
various
aspects
this
biomaterials.
This
concise
focuses
dipeptide
hydrogel
may
stimulate
current
trends
biomaterial
translate
its
products
for
applications.
Journal of Materials Chemistry B,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
A
novel
ultra-short
peptide
hydrogelator
capable
of
exhibiting
excellent
aggregation-induced
emission
and
biocompatible
features
that
have
been
exploited
for
in
vitro
cellular
imaging.
Gels,
Journal Year:
2024,
Volume and Issue:
10(1), P. 69 - 69
Published: Jan. 17, 2024
Spider
silk
proteins
(spidroins)
have
garnered
attention
in
biomaterials
research
due
to
their
ability
self-assemble
into
hydrogels.
However,
reported
spidroin
hydrogels
require
high
protein
concentration
and
prolonged
gelation
time.
Our
study
engineered
an
artificial
that
exhibits
unprecedented
rapid
self-assembly
at
physiologically
relevant
conditions,
achieving
a
low
of
6
mg/mL
37
°C
without
external
additives.
Remarkably,
30
concentration,
our
forms
within
s,
feature
we
termed
“superfast
gelation”.
This
formation
is
modulated
by
ions,
pH,
temperature,
offering
versatility
biomedical
applications.
The
hydrogel’s
capacity
encapsulate
support
E.
coli
growth
while
inducing
RFP
expression
provides
novel
platform
for
drug
delivery
bioengineering
findings
introduce
superfast,
highly
adaptable,
cytocompatible
hydrogel
self-assembles
under
mild
underscoring
the
practical
implication
clinical
ACS Applied Bio Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 21, 2025
Deciphering
the
most
promising
strategy
for
evolution
of
cancer
patient
management
remains
a
multifaceted,
challenging
affair
to
date.
Additionally,
such
approaches
often
lead
microbial
infections
as
side
effects,
probably
due
compromised
immunity
patients
undergoing
treatment.
Distinctly,
this
work
delineates
rational
combinatorial
harnessing
stereogenic
harmony
in
diphenylalanine
fragment,
tethering
it
an
amphiphile
12-hydroxy-lauric
acid
at
N-terminus
(compounds
I-III)
that
potential
therapeutic
could
be
extracted
out
from
series.
Aligned
goal,
cytotoxic
properties
and
cell
viability
compounds
were
investigated
using
two
distinct
lines:
MCF-7
(human
breast
cell)
HEK
293
embryonic
kidney).
Our
rigorous
investigations
revealed
I-III
exhibited
substantial
impact
on
MCF7
line.
But
pool
three
constructs,
compound
III
(12-hydroxy-lauric
-d-Phe-l-Phe-OH)
showed
better
selectivity
toward
cancerous
over
normal
comparison
others,
backed
by
computational
calculations.
Henceforth,
was
fished
series
used
its
elaborate
anticancer
activities
reactive
oxygen
species
generation,
DNA
fragmentation,
caspase-dependent
gene
expression
employing
extrinsic
intrinsic
apoptotic
factors
well
inflammatory
biomarkers,
namely,
TNF-α
IL1-β.
We
anticipated
III,
possessing
mechanoresponsiveness
nanofibrillar
network,
administered
with
injections
because
shear-thinning
properties.
Moreover,
optimum
partition
coefficient
might
have
allowed
scaffold
penetrate
cellular
membranes
form
dilactate
complex
(compound
VI)
when
exposed
accumulated
lactates/lactic
acids,
common
phenomenon
observed
within
hypoxic
tumor
cores,
accordance
Warburg
mechanism,
thereby
evading
cytotoxicity
cells.
Besides,
supramolecular
β-sheets
manifest
antimicrobial
efficacy
against
pathogens,
Gram-positive
bacteria,
S.
aureus
B.
subtilis,
Gram-negative
E.
coli
P.
aeruginosa,
fungus,
C.
albicans,
along
proteolytic
stability
high
mechanical
strength
physiological
pH.
Overall,
we
speculate
discovery
these
multifunctional
bioinspired
materials
holds
future
promise
preferential
therapeutics
remediation
immune-susceptible
patients,
afflicted
arising
alone
or
effects
chemotherapeutic
medications.
Marine Drugs,
Journal Year:
2025,
Volume and Issue:
23(4), P. 169 - 169
Published: April 15, 2025
Composite
hydrogels
with
improved
mechanical
and
chemical
properties
can
be
formed
by
non-covalently
decorating
the
nanofibrillar
structures
self-assembly
of
peptides
fucoidan.
Nevertheless,
precise
interactions,
electrochemical
thermodynamic
stability
these
composite
materials
have
not
been
determined.
Here,
we
present
a
analysis
interacting
forces
that
drive
formation
fucoidan/9-fluorenylmethoxycarbonyl-phenylalanine-arginine-glycine-aspartic
acid-phenylalanine
(Fmoc-FRGDF)
hydrogel.
The
results
showed
co-assembly
fucoidan
Fmoc-FRGDF
was
spontaneous
exothermic.
melting
point
increased
from
87.0
°C
to
107.7
for
8
mg/mL
added
A
complex
network
hydrogen
bonds
between
molecules
Fmoc-FRGDF,
electrostatic,
bond,
van
der
Waals
interactions
were
main
driving
Fmoc-FRGDF.
Furthermore,
sulfate
group
strong
salt
bridge
arginine
This
study
provides
useful
biomedical
engineering
design
parameters
inclusion
other
highly
soluble
biopolymers
into
types
hydrogel
vectors.
ACS Biomaterials Science & Engineering,
Journal Year:
2023,
Volume and Issue:
9(2), P. 784 - 796
Published: Jan. 24, 2023
Protein-based
therapeutics
have
emerged
as
next-generation
pharmaceutical
agents
for
oncology,
bone
regeneration,
autoimmune
disorders,
viral
infections,
and
other
diseases.
The
clinical
application
of
protein
has
been
impeded
by
pharmacokinetic
pharmacodynamic
challenges
including
off-target
toxicity,
rapid
clearance,
drug
stability.
Strategies
the
localized
sustained
delivery
shown
promise
in
addressing
these
challenges.
Hydrogels
are
critical
materials
that
enable
strategies.
Supramolecular
hydrogels
composed
self-assembled
demonstrated
biocompatibility
advantages
over
polymer
hydrogels,
with
peptide
protein-based
gels
showing
strong
potential.
However,
cost
is
a
significant
drawback
peptide-based
supramolecular
hydrogels.
inexpensive
low-molecular-weight
(LMW)
gelators,
modified
amino
acid
derivatives,
reported
viable
alternatives
to
materials.
Herein,
we
report
encapsulation
release
proteins
from
perfluorinated
fluorenylmethyloxcarbonyl-modified
phenylalanine
(Fmoc-F5-Phe-DAP).
Specifically,
demonstrate
four
model
(ribonuclease
A
(RNase
A),
trypsin
inhibitor
(TI),
bovine
serum
albumin
(BSA),
human
immunoglobulin
G
(IgG))
emergent
viscoelastic
properties
characterized,
functional
time-dependent
demonstrated.
In
addition,
it
aqueous
solution
used
hydrogel
formulation
influence
on
vitro
profiles,
function
isoelectric
point
molecular
weight
payloads.
These
studies
collectively
validate
this
class
LMW
possesses
requisite
therapeutics.
Gels,
Journal Year:
2024,
Volume and Issue:
10(8), P. 507 - 507
Published: Aug. 1, 2024
Self-assembled
peptide-based
hydrogels
have
attracted
considerable
interest
from
the
research
community.
Particularly,
low
molecular
weight
gelators
(LMWGs)
consisting
of
amino
acids
and
short
peptides
are
highly
suitable
for
biological
applications
owing
to
their
facile
synthesis
scalability,
as
well
biocompatibility,
biodegradability,
stability
in
physiological
conditions.
However,
challenges
understanding
structure–property
relationship
lack
design
rules
hinder
development
new
with
required
properties
several
applications.
Hereby,
plethora
gelators,
this
review
discusses
mechanical
single
acid
dipeptide-based
hydrogels.
A
mutual
analysis
these
systems
allows
us
highlight
between
gel
sequence,
preparation
methods,
or
N
capping
groups.
Additionally,
recent
advancements
tuning
gels’
rheological
reviewed.
In
way,
present
aims
help
bridge
knowledge
gap
structure
properties,
easing
selection
