Modern advances in spinal cord regeneration: hydrogel combined with neural stem cells DOI Creative Commons
Oksana Rybachuk,

Yuliia Nesterenko,

Valeriia Zhovannyk

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: June 27, 2024

Severe spinal cord injuries (SCI) lead to loss of functional activity the body below injury site, affect a person’s ability self-care and have direct impact on performance. Due structural features role in body, consequences SCI cannot be completely overcome at expense endogenous regenerative potential and, developing over time, severe complications years after injury. Thus, primary task this type treatment is create artificial conditions for growth damaged nerve fibers through area SCI. Solving problem possible using tissue neuroengineering involving technology replacing natural environment with synthetic matrices (for example, hydrogels) combination stem cells, particular, neural/progenitor cells (NSPCs). This approach can provide maximum stimulation support axons neurons their myelination. In review, we consider currently available options improving condition (use NSC transplantation or/and replacement matrix, specifically hydrogel). We emphasise expediency effectiveness hydrogel matrix + NSCs complex system used reconstruction Since such (a engineering cell therapy), our opinion, allows not only creation supporting regeneration or mechanical cord, but also strengthen regeneration, prevent spread inflammatory process, promote restoration lost reflex, motor sensory functions injured cord.

Language: Английский

3D bioprinting of a gradient stiffened gelatin–alginate hydrogel with adipose-derived stem cells for full-thickness skin regeneration DOI
Yuan Ma, Yilin Wang, Danni Chen

et al.

Journal of Materials Chemistry B, Journal Year: 2023, Volume and Issue: 11(13), P. 2989 - 3000

Published: Jan. 1, 2023

3D bioprinting of a gradient-stiffened gelatin–alginate hydrogel accelerated wound healing by promoting the proliferation, migration, and paracrine secretion encapsulated ADSCs.

Language: Английский

Citations

27
3D-Printing of Silk Nanofibrils Reinforced Alginate for Soft Tissue Engineering DOI Creative Commons

Zahra Mohammadpour,

Mahshid Kharaziha,

Ali Zarrabi

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(3), P. 763 - 763

Published: Feb. 24, 2023

The main challenge of extrusion 3D bioprinting is the development bioinks with desired rheological and mechanical performance biocompatibility to create complex patient-specific scaffolds in a repeatable accurate manner. This study aims introduce non-synthetic based on alginate (Alg) incorporated various concentrations silk nanofibrils (SNF, 1, 2, 3 wt.%) optimize their properties for soft tissue engineering. Alg-SNF inks demonstrated high degree shear-thinning reversible stress softening behavior contributing pre-designed shapes. In addition, our results confirmed good interaction between SNFs matrix resulted significantly improved biological characteristics controlled degradation rate. Noticeably, addition 2 wt.% SNF compressive strength (2.2 times), tensile (5 elastic modulus (3 times) alginate. reinforcing 3D-printed increased cell viability (1.5 proliferation (5.6 after 5 days culturing. summary, highlights favorable performances, rate, swelling, Alg-2SNF ink containing extrusion-based bioprinting.

Language: Английский

Citations

24
Advancements in hydrogel design for articular cartilage regeneration: A comprehensive review DOI Creative Commons

Fariba Hashemi-Afzal,

Hooman Fallahi, Fatemeh Bagheri

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 43, P. 1 - 31

Published: Sept. 14, 2024

Language: Английский

Citations

15
Highly oriented hydrogels for tissue regeneration: design strategies, cellular mechanisms, and biomedical applications DOI Creative Commons

Jiuping Wu,

Zhihe Yun,

Wenlong Song

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(5), P. 1982 - 2035

Published: Jan. 1, 2024

Many human tissues exhibit a highly oriented architecture that confers them with distinct mechanical properties, enabling adaptation to diverse and challenging environments. Hydrogels, their water-rich "soft wet" structure, have emerged as promising biomimetic materials in tissue engineering for repairing replacing damaged organs. Highly hydrogels can especially emulate the structural orientation found tissue, exhibiting unique physiological functions properties absent traditional homogeneous isotropic hydrogels. The design preparation of involve strategies like including nanofillers, polymer-chain networks, void channels, microfabricated structures. Understanding specific mechanism action how these affect cell behavior biological applications such cornea, skin, skeletal muscle, tendon, ligament, cartilage, bone, blood vessels, heart, etc., requires further exploration generalization. Therefore, this review aims fill gap by focusing on strategy application field engineering. Furthermore, we provide detailed discussion various organs mechanisms through which structures influence behavior.

Language: Английский

Citations

13
Design and characterization of 3D printed pore gradient hydrogel scaffold for bone tissue engineering DOI
Fariza Mukasheva, Muhammad Moazzam,

Bota Yernaimanova

et al.

Bioprinting, Journal Year: 2024, Volume and Issue: 39, P. e00341 - e00341

Published: April 10, 2024

Language: Английский

Citations

12
Injectable Hydrogels for Nervous Tissue Repair—A Brief Review DOI Creative Commons

Gladys Arline Politrón-Zepeda,

Gabriela Fletes-Vargas, Rogelio Rodríguez‐Rodríguez

et al.

Gels, Journal Year: 2024, Volume and Issue: 10(3), P. 190 - 190

Published: March 9, 2024

The repair of nervous tissue is a critical research field in engineering because the degenerative process injured system. In this review, we summarize progress injectable hydrogels using vitro and vivo studies for regeneration tissue. Traditional treatments have not been favorable patients, as they are invasive inefficient; therefore, promising treatment damaged This review will contribute to better understanding potential scaffolds drug delivery system neural applications.

Language: Английский

Citations

10
Adipose-derived stem cells loaded photocurable and bioprintable bioinks composed of GelMA, HAMA and PEGDA crosslinker to differentiate into smooth muscle phenotype DOI
Pavanchandh Atturu, Sunaina Mudigonda,

Chau‐Zen Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130710 - 130710

Published: March 16, 2024

Language: Английский

Citations

9
Microgel-based bioink for extrusion-based 3D bioprinting and its applications in tissue engineering DOI

Keerthi Subramanian Iyer,

Lei Bao, Jiali Zhai

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 48, P. 273 - 293

Published: Feb. 20, 2025

Language: Английский

Citations

1
Injectable and Microporous Microgel‐Fiber Granular Hydrogel Loaded with Bioglass and siRNA for Promoting Diabetic Wound Healing DOI Creative Commons
Ying Li, Wei Song,

Lingzhi Kong

et al.

Small, Journal Year: 2023, Volume and Issue: 20(19)

Published: Dec. 6, 2023

Abstract Injectable hydrogels find extensive application in the treatment of diabetic wound healing. However, traditional bulk are significantly limited due to their nano‐porous structure, which obstructs cell migration and tissue infiltration. Moreover, regulating inflammation matrix metalloproteinase ‐9 (MMP‐9) expression wounds is crucial for enhancing This study marks first instance introducing an efficient, scalable, simple method producing microfiber‐gel granules encapsulating bioceramics powders. Utilizing this method, injectable microporous granular microgel‐fiber hydrogel (MFgel) successfully developed by assembling microgel‐fibers made from hyaluronic acid (HA) sodium alginate (SA) loaded with small interfering RNA (siRNA) bioglass (BG) particles. Compared (Tgel), MFgel possesses a highly interconnected network micron‐sized pores, demonstrating favorable properties adhesion penetration vitro experiments. Additionally, exhibits higher compressive modulus superior mechanical stability. When implanted subcutaneously mice, promotes cellular infiltration, facilitating proliferation. Furthermore, when applied skin defects rats, not only effectively regulates suppresses MMP‐9 but also enhances angiogenesis collagen deposition, thereby accelerating Taken together, great potential healing applications.

Language: Английский

Citations

22
Nanofiber Composite Microchannel‐Containing Injectable Hydrogels for Cartilage Tissue Regeneration DOI
Jia Liu,

Chen Tang,

Jian Huang

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(31)

Published: Sept. 10, 2023

Abstract Articular cartilage tissue is incapable of self‐repair and therapies for defects are still lacking. Injectable hydrogels have drawn much attention in the field regeneration. Herein, novel design nanofiber composite microchannel‐containing inspired by tunnel‐piled structure subway tunnels proposed. Based on aldehydized polyethylene glycol/carboxymethyl chitosan (APA/CMCS) hydrogels, thermosensitive gelatin microrods (GMs) used as a pore‐forming agent, coaxial electrospinning polylactic acid/gelatin fibers (PGFs) loaded with kartogenin (KGN) reinforcing agent drug delivery system to construct injectable (APA/CMCS/KGN@PGF/GM hydrogels). The situ formation, micromorphology porosity, swelling degradation, mechanical properties, self‐healing behavior, well release investigated. hydrogel exhibits good ability, introduction PGF nanofibers can significantly improve properties. realize sustained KGN match process repair. microchannel effectively promotes bone marrow mesenchymal stem cell (BMSC) proliferation ingrowth within hydrogels. In vitro animal experiments indicate that APA/CMCS/KGN@PGF/GM enhance chondrogenesis BMSCs promote neocartilage formation rabbit defect model.

Language: Английский

Citations

19