Genome engineering in barley DOI

Hajra Maqsood,

Arzoo Ahad,

Somia Khan

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 257 - 272

Published: Jan. 1, 2024

An overview of genome engineering in plants, including its scope, technologies, progress and grand challenges DOI
Muhammad Sufyan, Umar Daraz, Sajjad Hyder

et al.

Functional & Integrative Genomics, Journal Year: 2023, Volume and Issue: 23(2)

Published: April 6, 2023

Language: Английский

Citations

42

Melatonin-Induced Stress Enhanced Biomass and Production of High-Value Secondary Cell Products in Submerged Adventitious Root Cultures of Stevia rebaudiana (Bert.) DOI Creative Commons

Bakht Naz,

Aftab Afzal, Hina Ali

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(5), P. 5548 - 5562

Published: Jan. 26, 2024

Stress is one of the important factors that directly or indirectly affects plant architecture, biochemical pathways, and growth development. Melatonin (MEL) an stress hormone; however, exogenous addition melatonin to culture media stimulates defense mechanism releases higher quantities secondary metabolites. In this study, submerged adventitious root cultures (SARCs) diabetically

Language: Английский

Citations

8

Potential of Thermal and RGB Imaging Combined with Artificial Neural Networks for Assessing Salt Tolerance of Wheat Genotypes Grown in Real-Field Conditions DOI Creative Commons
Salah El-Hendawy, Muhammad Usman Tahir,

Nasser Al-Suhaibani

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(7), P. 1390 - 1390

Published: June 27, 2024

Developing new bread wheat varieties that can be successfully grown in saline conditions has become a pressing task for plant breeders. High-throughput phenotyping tools are crucial this task. Proximal remote sensing is gaining popularity breeding programs as quick, cost-effective, and non-invasive tool to assess canopy structure physiological traits large genetic pools. Limited research been conducted on the effectiveness of combining RGB thermal imaging salt tolerance different genotypes. This study aimed evaluate several indices derived from infrared images with artificial neural networks (ANNs) assessing relative water content (RWC), chlorophyll (Chla), b (Chlb), total (Chlt), dry weight (PDW) 18 recombinant inbred lines (RILs) their 3 parents irrigated (150 mM NaCl). The results showed significant differences various among tested normalized temperature (NRCT) index exhibited strong correlations RWC, Chla, Chlb, Chlt, PDW, R2 values ranging 0.50 0.73, 0.53 0.76, 0.68 0.84, 0.52 respectively. Additionally, there was relationship between measured traits, highest reaching up 0.70. visible atmospherically resistant (VARI), popular imaging, NRCT, 0.49 0.62 across two seasons. ANNs models demonstrated high predictive accuracy NRCT other 0.90 training dataset 0.46 cross-validation dataset. Thus, our shows integrating high-throughput digital image ANN efficiently non-invasively number genotypes programs.

Language: Английский

Citations

7

Water stress effects on stay green and chlorophyll fluorescence with focus on yield characteristics of diverse bread wheats DOI
Ahmad Ali, Zahid Ullah, Hassan Sher

et al.

Planta, Journal Year: 2023, Volume and Issue: 257(6)

Published: April 28, 2023

Language: Английский

Citations

13

Biochemical and phenological characterization of diverse wheats and their association with drought tolerance genes DOI Creative Commons
Iftikhar Ali, Saeed Anwar, Ahmad Ali

et al.

BMC Plant Biology, Journal Year: 2023, Volume and Issue: 23(1)

Published: June 19, 2023

Drought is one of the most important wheat production limiting factor, and can lead to severe yield losses. This study was designed examine effect drought stress on physiology morphology under three different field capacities (FC) viz. 80% (control), 50% (moderate) 30% (severe stress) in a diverse collection germplasm including cultivars, landraces, synthetic hexaploid their derivatives. Traits like grain weight, thousand weight biomass were reduced by 38.23%, 18.91% 26.47% respectively at FC, whereas reduction rate for these traits FC 19.57%, 8.88% 18.68%. In principal component analysis (PCA), first two components PC1 PC2 accounted 58.63% total variation separated cultivars landraces from synthetic-based germplasm. Landraces showed wide range phenotypic variations compared improved cultivars. However, least observed which indicated progress developing resilient Allelic drought-related genes TaSnRK2.9-5A, TaLTPs-11, TaLTPs-12, TaSAP-7B-, TaPPH-13, Dreb-B1 1fehw3 significantly associated with phenological all 91 wheats 40 9 varieties, 34 hexaploids 8 The favorable haplotypes 1fehw3, Dreb-B1, TaLTPs-11 TaLTPs-12 increased biomass. Our results iterated fact that could be promising source deploy adaptability breeding. further identified tolerant genetic resources across various backgrounds favourable water-saving should considered develop varieties.

Language: Английский

Citations

9

The Impact of Trichoderma harzianum Together with Copper and Boron on Wheat Yield DOI
Saeed Anwar, Ahmad Ali, Zahid Ullah

et al.

ACS Agricultural Science & Technology, Journal Year: 2023, Volume and Issue: 3(6), P. 517 - 527

Published: May 18, 2023

Deficiency of micronutrients is a major problem faced by nearly half the world population due to intake poor-quality food. To overcome malnutrition, biofortification cereal crops believed be good approach increase nutrient contents in our daily The purpose current study was therefore investigate effect foliar applied copper and boron alone combination with Trichoderma harzianum on yield attributes, grain quality, wheat. Wheat plants were treated or without T. harzianum. treatments included Cu (0.05 M), (0.1 harzianum, M)/T. B M)/B along control set for comparison. Results revealed significant enhancement different studied growth traits including plant height, spike length, kernels per spike, harvest index, chlorophyll content. In addition, this also enriched wheat grains various micro/macronutrients Cu, Fe, Zn, K, P. This concludes that bioreagent may lead reasonably enhanced quality characteristics wheat, further suggesting combined application under suitable conditions will make it an acceptable crop improvements.

Language: Английский

Citations

7

Regulation of an endophytic nitrogen-fixing bacteria GXS16 promoting drought tolerance in sugarcane DOI Creative Commons

Qian Nong,

Li‐Jen Lin,

Jinlan Xie

et al.

BMC Plant Biology, Journal Year: 2023, Volume and Issue: 23(1)

Published: Nov. 17, 2023

Abstract Background Drought limits crop growth and is an important issue in commercial sugarcane ( Saccharum officinarum ) production. tolerance induced by endophytic nitrogen-fixing bacteria a complex biological process that ranges from altered gene expression cellular metabolism to changes productivity. Results In this study, physiological features transcriptome related drought conferred the Burkholderia bacterial strain GXS16 were investigated. Sugarcane samples inoculated with exhibited significantly higher leaf relative water content than those without inoculation during stages. treated had lower levels of H 2 O abscisic acid not non-watering groups. Transcriptomic analysis roots identified multiple differentially expressed genes between adjacent stages under different treatments. Moreover, both trend weighted correlation network analyses revealed carotenoid biosynthesis, terpenoid backbone starch sucrose metabolism, plant hormone signal transduction strongly contributed drought-tolerant phenotype treatment. Accordingly, regulatory including four regulated biosynthesis crtB , crtZ ZEP CYP707A three dxs dxr PCME was constructed. Conclusions This study provides insights into molecular mechanisms underlying application treatment enhance sugarcane, which will lay foundation for development improve

Language: Английский

Citations

7

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches DOI Creative Commons
Muhammad Ilyas, Iftikhar Ali, Dalal Nasser Binjawhar

et al.

ACS Omega, Journal Year: 2023, Volume and Issue: 8(18), P. 16327 - 16344

Published: April 26, 2023

Germin (GER) and germin-like proteins (GLPs) play an important role in various plant processes. Zea mays contains 26 protein genes (ZmGLPs) located on chromosomes 2, 4, 10; most of which are functionally unexplored. The present study aimed to characterize all ZmGLPs using the latest computational tools. All them were studied at a physicochemical, subcellular, structural, functional level, their expression was predicted development, against biotic abiotic stresses silico approaches. Overall, showed greater similarity physicochemical properties, domain architecture, structure, mostly localized cytoplasmic or extracellular regions. Phylogenetically, they have narrow genetic background with recent history gene duplication events chromosome 4. Functional analysis revealed novel enzymatic activities phosphoglycolate phosphatase, adenosylhomocysteinase, phosphatase-like, osmotin/thaumatin-like, acetohydroxy acid isomeroreductase largely mediated by disulfide bonding. Expression crucial root, root tips, crown elongation maturation zones, radicle, cortex highest being observed during germination maturity levels. Further, strong (Aspergillus flavus, Colletotrichum graminicola, Cercospora zeina, Fusarium verticillioides, virguliforme) while limited noted stresses. Concisely, our results provide platform for additional exploration ZmGLP environmental

Language: Английский

Citations

5

Integrating Hyperspectral Reflectance-Based Phenotyping and SSR Marker-Based Genotyping for Assessing the Salt Tolerance of Wheat Genotypes under Real Field Conditions DOI Creative Commons
Salah El-Hendawy, Muhammad Bilawal Junaid,

Nasser Al-Suhaibani

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(18), P. 2610 - 2610

Published: Sept. 19, 2024

Wheat breeding programs are currently focusing on using non-destructive and cost-effective hyperspectral sensing tools to expeditiously accurately phenotype large collections of genotypes. This approach is expected accelerate the development abiotic stress tolerance genotypes in programs. study aimed assess salt wheat canopy spectral reflectance measurements as an alternative direct laborious time-consuming phenological selection criteria. Eight sixteen F8 RILs were tested under 150 mM NaCl real field conditions for two years. Fourteen indices (SRIs) calculated from data, including vegetation SRIs water SRIs. The effectiveness these assessing was compared with four morpho-physiological traits genetic parameters, SSR markers, Mantel test, hierarchical clustering heatmaps, stepwise multiple linear regression, principal component analysis (PCA). results showed significant differences (p ≤ 0.001) among RILs/cultivars both heritability, gain, genotypic phenotypic coefficients variability most comparable those measured traits. effectively differentiated between salt-tolerant sensitive exhibited strong correlations markers (R2 = 0.56–0.89), similar allelic data 34 SSRs. A correlation (r 0.27, p < 0.0001) found similarity which higher than that 0.20, 0.0003) based test. PCA indicated all grouped a positive direction identified RILs/cultivars. PLSR models, SRIs, robustly estimated various individual suggests can be integrated tool phenotyping screening populations short time frame. replace need traditional

Language: Английский

Citations

1

Screening Diverse Aegilops tauschii for osmotic stress tolerance through physio-biochemical and anatomical characterization DOI
Muhammad Ayaz, Ahmad Ali, Zahid Ullah

et al.

Genetic Resources and Crop Evolution, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Language: Английский

Citations

1