Melatonin and cardioprotection against ischaemia/reperfusion injury: What's new? A review

Journal of Pineal Research, Journal Year: 2018, Volume and Issue: 65(1)

Published: March 23, 2018

Abstract Melatonin is a pleiotropic hormone with several functions. It binds to specific receptors and number of cytosolic proteins, activating vast array signalling pathways. Its potential protect the heart against ischaemia/reperfusion damage has attracted much attention, particularly in view its possible clinical applications. This review will focus mainly on pathways involved melatonin‐induced cardioprotection. In particular, role melatonin events downstream receptor activation, for example, reperfusion injury salvage kinase ( RISK ), survivor factor enhancement SAFE ) Notch pathways, sirtuins, nuclear E2–related 2 (Nrf2) translocases outer membrane TOM 70) be discussed. Particular attention given mitochondrion addition, brief overview regarding status quo application humans.

Language: Английский

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Endothelial cells support osteogenesis in an in vitro vascularized bone model developed by 3D bioprinting

Biofabrication, Journal Year: 2020, Volume and Issue: 12(2), P. 025013 - 025013

Published: Jan. 13, 2020

Abstract Bone is a highly vascularized tissue, in which vascularization and mineralization are concurrent processes during skeletal development. Indeed, both components should be included any reliable adherent vitro model platform for the study of bone physiology pathogenesis disorders. To this end, we developed an model, using gelatin-nanohydroxyapatite (gel-nHA) three-dimensional (3D) bioprinted scaffold. First, seeded human mesenchymal stem cells (hMSCs) on scaffold, underwent osteogenic differentiation 2 weeks. Then, lentiviral-GFP transfected umbilical vein endothelial (HUVECs) within 3D scaffold macropores to form capillary-like network more weeks culture. We tested three experimental conditions: condition 1, constructs with HUVECs cultured 1:1 medium (OM): (EM); 2, without OM:EM; 3: construct growth medium:EM. All samples resulted engineered matrix. In conditions 1 3, formed tubular structures constructs, assembly complex visible by fluorescence microscopy live tissue histology. CD31 immunostaining confirmed significant vascular lumen formation. Quantitative real-time PCR was used quantify response. Alkaline phosphatase runt-related transcription factor upregulation early commitment hMSCs. Even when OM removed under observed clear osteogenesis, notably accompanied osteopontin, factor, collagen type I. These findings indicate that have successfully realized robust just 4 culture highlighted how inclusion realistically supports osteogenesis. The approach reported here biologically inspired vascularization, simulating de novo morphogenesis capillary vessels occurring

Language: Английский

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The dual role of the glycosaminoglycan chondroitin‐6‐sulfate in the development, progression and metastasis of cancer

FEBS Journal, Journal Year: 2019, Volume and Issue: 286(10), P. 1815 - 1837

Published: Jan. 14, 2019

The remarkable structural heterogeneity of chondroitin sulfate (CS) and dermatan (DS) generates biological information that can be unique to each these glycosaminoglycans (GAGs), changes in their composition are translated into alterations the binding profiles molecules. CS/DS bind various cytokines growth factors, cell surface receptors, adhesion molecules, enzymes fibrillar glycoproteins extracellular matrix, thereby influencing both behavior biomechanical biochemical properties matrix. In this review, we summarize current knowledge concerning metabolism human cancer stroma. remodeling GAG profile tumor niche is manifested as a substantial increase CS content gradual decrease proportion between DS CS. Furthermore, also affected, which results 6-O-sulfated and/or unsulfated disaccharide content, concomitant with 4-O-sulfation level. Here, discuss possible impact sulfation pattern on capacity specificity GAGs. Moreover, propose potential consequences stromal accumulation chondroitin-6-sulfate for progression metastasis cancer.

Language: Английский

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Cytotoxicity of targeted PLGA nanoparticles: a systematic review

RSC Advances, Journal Year: 2021, Volume and Issue: 11(16), P. 9433 - 9449

Published: Jan. 1, 2021

Recent advances in nanotechnology have contributed tremendously to the development and revolutionizing of drug delivery systems field nanomedicine. In particular, targeting nanoparticles based on biodegradable poly(lactic-co-glycolic acid) (PLGA) polymers gained much interest. However, PLGA remain concern for their effectiveness against cancer cells toxicity normal cells. The aim this systematic review is identify a promising nanoformulation comparison study cytotoxicity potency different cell lines. A literature search was conducted through databases Google Scholar, PubMed, ScienceDirect, Scopus SpringerLink. sources studied were published between 2009 2019, variety keywords utilized. total, 81 manuscripts that met inclusion exclusion criteria selected analysis cytotoxicity, size, zeta potential, year publication, type ligand, active compounds line used. half maximal inhibitory concentration (IC50) main measurement data extraction, SI units standardized μg mL-1 better view comparison. This also identified inversely proportional nanoparticle size. predominantly exhibited size less than 300 nm absolute potential ∼20 mV. conclusion, more comprehensive critical appraisals pharmacokinetic, toxicokinetic, vivo vitro tests are required investigation full value treatment.

Language: Английский

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An update on the role of Hippo signaling pathway in ischemia-associated central nervous system diseases

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 162, P. 114619 - 114619

Published: March 31, 2023

The most frequent reason of morbidity and mortality in the world, cerebral ischemia sets off a chain molecular cellular pathologies that associated with some central nervous system (CNS) disorders mainly including ischemic stroke, Alzheimer's disease (AD), Parkinson's (PD), epilepsy other CNS diseases. In recent times, despite significant advancements treatment pathological processes underlying various neurological illnesses, effective therapeutic approaches are specifically targeted to minimizing damage such diseases remain absent. Hippo signaling pathway, characterized by enzyme linked reactions between MSTI/2, LAST1/2, YAP or TAZ proteins, controls cell division, survival, differentiation, as well being engaged variety biological activities, development transformation system. Recently, accumulating studies demonstrated pathway takes part AD, PD, etc., but not limited oxidative stress, inflammatory response, blood-brain barrier damage, mitochondrial disorders, neural cells death. Thus, it's crucial understand basis for determining potential new targets against ischemia-associated Here, we discuss latest advances deciphering highlight targeting treating

Language: Английский

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The highly unnatural fatty acid profile of cells in culture

Progress in Lipid Research, Journal Year: 2019, Volume and Issue: 77, P. 101017 - 101017

Published: Dec. 3, 2019

Language: Английский

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Dietary phospholipids: Role in cognitive processes across the lifespan

Neuroscience & Biobehavioral Reviews, Journal Year: 2020, Volume and Issue: 111, P. 183 - 193

Published: Jan. 13, 2020

Chronic stress and ageing are two of the most important factors that negatively affect cognitive processes such as learning memory across lifespan. To date, pharmacological agents have been insufficient in reducing impact both on brain health, thus, novel therapeutic strategies required. Recent research has focused nutritional interventions to modify behaviour reduce deleterious consequences ageing. In this context, emerging evidence indicate phospholipids, a specific type fat, capable improving variety animals humans. The mechanisms underlying these positive effects actively being investigated but yet not fully elucidated. review, we summarise preclinical clinical studies available phospholipid-based for improved health Moreover, summarize hypothesized direct indirect action lipid-based which may be used promote resilience improve age-related decline vulnerable populations.

Language: Английский

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Immunomodulatory Properties of Plants and Mushrooms

Trends in Pharmacological Sciences, Journal Year: 2017, Volume and Issue: 38(11), P. 967 - 981

Published: Aug. 30, 2017

Language: Английский

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Liquid‐liquid phase separation and fibrillation of the prion protein modulated by a high‐affinity DNA aptamer

The FASEB Journal, Journal Year: 2019, Volume and Issue: 34(1), P. 365 - 385

Published: Nov. 22, 2019

Structural conversion of cellular prion protein (PrPC) into scrapie PrP (PrPSc) and subsequent aggregation are key events associated with the onset transmissible spongiform encephalopathies (TSEs). Experimental evidence supports role nucleic acids (NAs) in assisting this conversion. Here, we asked whether undergoes liquid-liquid phase separation (LLPS) if process is modulated by NAs. To end, two 25-mer DNA aptamers, A1 A2, were selected against globular domain recombinant murine (rPrP90-231) using SELEX methodology. Multiparametric structural analysis these aptamers revealed that adopts a hairpin conformation. Aptamer binding caused partial unfolding rPrP90-231 its ability to undergo LLPS fibrillate. In fact, although free separated large droplets, aptamer increased number droplets but noticeably reduced their size. Strikingly, modified does not adopt structure induced formation amyloid fibrils on surface droplets. We show here LLPS, interaction NAs modulates promotes fibrillation NA concentration-dependent manner. These results shed new light roles misfolding TSEs.

Language: Английский

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The emergence of melatonin in oncology: Focus on colorectal cancer

Medicinal Research Reviews, Journal Year: 2019, Volume and Issue: 39(6), P. 2239 - 2285

Published: April 4, 2019

Within the last few decades, melatonin has increasingly emerged in clinical oncology as a naturally occurring bioactive molecule with substantial anticancer properties and pharmacological profile optimal for joining currently available pharmacopeia. In addition, extensive experimental data shows that this chronobiotic agent exerts oncostatic effects throughout all stages of tumor growth, from initial cell transformation to mitigation malignant progression metastasis; additionally, alleviates side improves welfare radio/chemotherapy-treated patients. Thus, support clinicians oncologists use both treatment proactive prevention cancer is gaining strength. Because its epidemiological importance symptomatic debut advanced difficult management, colorectal (CRC) preferential target testing new therapies. regard, development effective forms intervention improvement CRC outcome, specifically metastatic CRC, urgent. At same time, need reduce costs conventional anti-CRC therapy results also imperative. light status quo, therapeutic potential melatonin, direct indirect critical processes malignancy it modulates, have aroused much interest. To illuminate imminent future on research, we focused our attention molecular mechanisms underlying multiple actions displayed by onset evolution summarized evidence, well vitro, vivo findings broadly protective demonstrated melatonin.

Language: Английский

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