Analysis of the Robotic-Based In Situ Bioprinting Workflow for the Regeneration of Damaged Tissues through a Case Study DOI Creative Commons
Gabriele Maria Fortunato,

Sofia Sigismondi,

Matteo Nicoletta

et al.

Bioengineering, Journal Year: 2023, Volume and Issue: 10(5), P. 560 - 560

Published: May 8, 2023

This study aims to critically analyse the workflow of in situ bioprinting procedure, presenting a simulated neurosurgical case study, based on real traumatic event, for collecting quantitative data support this innovative approach. After event involving head, bone fragments may have be removed and replacement implant placed through highly demanding surgical procedure terms surgeon dexterity. A promising alternative current technique is use robotic arm deposit biomaterials directly onto damaged site patient following planned curved surface, which can designed pre-operatively. Here we achieved an accurate planning-patient registration pre-operative fiducial markers positioned around area, reconstructed starting from computed tomography images. Exploiting availability multiple degrees freedom regeneration complex also overhanging parts typical anatomical defects, work platform IMAGObot was used regenerate cranial defect patient-specific phantom. The process then successfully performed showing great potential technology field surgery. In particular, accuracy deposition quantified, as well duration whole compared standard practice. Further investigations include biological characterisation over time printed construct vitro vivo analysis proposed approach, better biomaterial performances osteo-integration with native tissue.

Language: Английский

Strategies to use fibrinogen as bioink for 3D bioprinting fibrin-based soft and hard tissues DOI
Bruna Alice Gomes de Melo, Yasamin A. Jodat, Elisa M. Cruz

et al.

Acta Biomaterialia, Journal Year: 2020, Volume and Issue: 117, P. 60 - 76

Published: Sept. 16, 2020

Language: Английский

Citations

161
3D Bioprinting of Vascularized Tissues for in vitro and in vivo Applications DOI Creative Commons
Earnest P. Chen,

Zeren Toksoy,

Bruce A. Davis

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2021, Volume and Issue: 9

Published: May 13, 2021

With a limited supply of organ donors and available organs for transplantation, the aim tissue engineering with three-dimensional (3D) bioprinting technology is to construct fully functional viable replacements various clinical applications. 3D allows customization complex architecture numerous combinations materials printing methods build different types, eventually replacement organs. The main challenge maintaining printed viability inclusion vascular networks nutrient transport waste disposal. Rapid development discoveries in recent years have taken huge strides toward perfecting incorporation In this review, we will discuss latest advancements fabricating vascularized including novel strategies materials, their Our discussion begin exploration vasculature, progress through current statuses tissue/organoids from bone muscles organs, conclude relevant applications vitro models drug testing. We also explore limitations some promising future directions may bring.

Language: Английский

Citations

118
Vascularization Strategies in Bone Tissue Engineering DOI Creative Commons
Filip Šimunović, Günter Finkenzeller

Cells, Journal Year: 2021, Volume and Issue: 10(7), P. 1749 - 1749

Published: July 11, 2021

Bone is a highly vascularized tissue, and its development, maturation, remodeling, regeneration are dependent on tight regulation of blood vessel supply. This condition also has to be taken into consideration in the context development artificial tissue substitutes. In classic engineering, bone-forming cells such as primary osteoblasts or mesenchymal stem introduced suitable scaffolds implanted order treat critical-size bone defects. However, substitutes initially avascular. Because occurrence hypoxic conditions, especially larger substitutes, this leads death cells. Therefore, it necessary devise vascularization strategies aiming at fast efficient tissues. review article, we present discuss current engineering. These based use angiogenic growth factors, co-implantation forming cells, ex vivo microfabrication vessels by means bioprinting, surgical methods for creating surgically transferable composite

Language: Английский

Citations

112
Fabrication and characterization of mechanically competent 3D printed polycaprolactone-reduced graphene oxide scaffolds DOI Creative Commons
Amir Seyedsalehi, Leila Daneshmandi, Mohammed A. Barajaa

et al.

Scientific Reports, Journal Year: 2020, Volume and Issue: 10(1)

Published: Dec. 17, 2020

Abstract The ability to produce constructs with a high control over the bulk geometry and internal architecture has situated 3D printing as an attractive fabrication technique for scaffolds. Various designs inks are actively investigated prepare scaffolds different tissues. In this work, we prepared printed composite comprising polycaprolactone (PCL) various amounts of reduced graphene oxide (rGO) at 0.5, 1, 3 wt.%. We employed two-step process ensure even mixture distribution rGO sheets within PCL matrix. were by creating PCL-rGO films through solvent evaporation casting that subsequently fed into printer extrusion. resultant seamlessly integrated, fidelity consistency across all groups. This, together homogeneous dispersion polymer matrix, significantly improved compressive strength stiffness 185% 150%, respectively, 0.5 wt.% inclusion. in vitro response was assessed using human adipose-derived stem cells. All cytocompatible supported cell growth viability. These mechanically reinforced biologically compatible promising platform regenerative engineering applications.

Language: Английский

Citations

100
Applications of 3D bioprinting in tissue engineering: advantages, deficiencies, improvements, and future perspectives DOI
Baosen Tan,

Shaolei Gan,

Xiumei Wang

et al.

Journal of Materials Chemistry B, Journal Year: 2021, Volume and Issue: 9(27), P. 5385 - 5413

Published: Jan. 1, 2021

3D bioprinting has progressed tremendously in the field of tissue engineering its ability to deliver cells with high precision, prototypes rapidly customized features, and engineer constructs highly controllable microenvironments.

Language: Английский

Citations

83
In Vitro Strategies to Vascularize 3D Physiologically Relevant Models DOI Creative Commons
Alessandra Dellaquila, Chau Le Bao, Didier Letourneur

et al.

Advanced Science, Journal Year: 2021, Volume and Issue: 8(19)

Published: Aug. 5, 2021

Abstract Vascularization of 3D models represents a major challenge tissue engineering and key prerequisite for their clinical industrial application. The use prevascularized built from dedicated materials could solve some the actual limitations, such as suboptimal integration bioconstructs within host tissue, would provide more in vivo‐like perfusable organ‐specific platforms. In last decade, fabrication vascularized physiologically relevant constructs has been attempted by numerous strategies, which are classified here microfluidic technology, coculture models, namely, spheroids organoids, biofabrication. this review, recent advancements prevascularization techniques increasing natural synthetic to build physiological discussed. Current drawbacks each future perspectives, translation toward clinics, pharmaceutical field, industry also presented. By combining complementary these envisioned be successfully used regenerative medicine drug development near future.

Language: Английский

Citations

79
3D Bioprinting for Vascularized Tissue-Engineered Bone Fabrication DOI Open Access
Fei Xing,

Zhou Xiang,

Pol Maria Rommens

et al.

Materials, Journal Year: 2020, Volume and Issue: 13(10), P. 2278 - 2278

Published: May 15, 2020

Vascularization in bone tissues is essential for the distribution of nutrients and oxygen, as well removal waste products. Fabrication tissue-engineered constructs with functional vascular networks has great potential biomimicking nature tissue vitro enhancing regeneration vivo. Over past decades, many approaches have been applied to fabricate biomimetic vascularized constructs. However, traditional methods based on seeding cells into scaffolds are unable control spatial architecture encapsulated cell precisely, which posed a significant challenge constructing complex precise properties. In recent years, pioneering technology, three-dimensional (3D) bioprinting technology multiscale, biomimetic, multi-cellular highly microenvironment through layer-by-layer printing. This review discussed application 3D fabrication, where current status unique challenges were critically reviewed. Furthermore, mechanisms formation, process bioprinting, development bioink properties also discussed.

Language: Английский

Citations

76
Dual 3D printing for vascularized bone tissue regeneration DOI

Sung Yun Hann,

Haitao Cui, Timothy Esworthy

et al.

Acta Biomaterialia, Journal Year: 2021, Volume and Issue: 123, P. 263 - 274

Published: Jan. 14, 2021

Language: Английский

Citations

68
Higher yield and enhanced therapeutic effects of exosomes derived from MSCs in hydrogel-assisted 3D culture system for bone regeneration DOI Open Access
Wenting Yu, Shengnan Li, X. Guan

et al.

Biomaterials Advances, Journal Year: 2022, Volume and Issue: 133, P. 112646 - 112646

Published: Jan. 7, 2022

Language: Английский

Citations

63
3D bioprinting of in situ vascularized tissue engineered bone for repairing large segmental bone defects DOI Creative Commons
Mingkui Shen, Lulu Wang, Yi Gao

et al.

Materials Today Bio, Journal Year: 2022, Volume and Issue: 16, P. 100382 - 100382

Published: Aug. 8, 2022

Large bone defects remain an unsolved clinical challenge because of the lack effective vascularization in newly formed tissue. 3D bioprinting is a fabrication technology with potential to create vascularized grafts biological activity for repairing defects. In this study, vascular endothelial cells laden thermosensitive bio-ink were bioprinted situ on inner surfaces interconnected tubular channels mesenchymal stem cell-laden 3D-bioprinted scaffolds. Endothelial exhibited more uniform distribution and greater seeding efficiency throughout channels. vitro, can form network through proliferation migration. The tissue-engineered also resulted coupling effect between angiogenesis osteogenesis. Moreover, RNA sequencing analysis revealed that expression genes related osteogenesis upregulated processes. vivo scaffolds excellent performance promoting new formation rat calvarial critical-sized defect models. Consequently, bones constructed using have being used as large defects, possible application future.

Language: Английский

Citations

52