Roles of Exosomal miRNAs in Asthma: Mechanisms and Applications DOI Creative Commons
Xiaoxue Liu, Jiawei Gao,

Liuxin Yang

et al.

Journal of Asthma and Allergy, Journal Year: 2024, Volume and Issue: Volume 17, P. 935 - 947

Published: Sept. 1, 2024

Asthma is a chronic inflammatory disorder of the airways, characterized by complex interplay genetic, environmental, and immunological factors that contribute to its onset progression. Recent advances in researches have illuminated critical role exosomal microRNAs (miRNAs) pathogenesis development asthma. Exosomes are nano-sized extracellular vesicles facilitate intercellular communication transporting variety bioactive molecules, including miRNAs, play crucial regulating gene expression immune responses, which central processes underlying Exosomal miRNAs emerging as key players asthma due their involvement various aspects disease, regulation inflammation, airway hyperresponsiveness, remodeling. Their ability influence behavior target cells tissues makes them valuable both diagnostic biomarkers potential therapeutic targets. This review aims provide comprehensive overview biogenesis exosomes, functional roles asthma, clinical potential. It will explore mechanisms these pathophysiology, discuss utility diagnosing monitoring highlight ongoing research efforts harness

Language: Английский

The gut–airway microbiome axis in health and respiratory diseases DOI
Mustafa Özçam, Susan V. Lynch

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(8), P. 492 - 506

Published: May 22, 2024

Language: Английский

Citations

27
Understanding exosomes: Part 2—Emerging leaders in regenerative medicine DOI Creative Commons
Richard J. Miron, Nathan E. Estrin, Anton Sculean

et al.

Periodontology 2000, Journal Year: 2024, Volume and Issue: 94(1), P. 257 - 414

Published: Feb. 1, 2024

Abstract Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with ability to communicate other tissues and cell types over long distances. Their use in regenerative medicine has gained tremendous momentum recently due their be utilized as therapeutic options for a wide array diseases/conditions. Over 5000 publications currently being published yearly on this topic, number is only expected dramatically increase novel strategies continue developed. Today exosomes have been applied numerous contexts including neurodegenerative disorders (Alzheimer's disease, central nervous system, depression, multiple sclerosis, Parkinson's post‐traumatic stress disorders, traumatic brain injury, peripheral nerve injury), damaged organs (heart, kidney, liver, stroke, myocardial infarctions, ovaries), degenerative processes (atherosclerosis, diabetes, hematology musculoskeletal degeneration, osteoradionecrosis, respiratory disease), infectious diseases (COVID‐19, hepatitis), procedures (antiaging, bone regeneration, cartilage/joint osteoarthritis, cutaneous wounds, dental dermatology/skin erectile dysfunction, hair regrowth, intervertebral disc repair, spinal cord vascular regeneration), cancer therapy (breast, colorectal, gastric osteosarcomas), immune function (allergy, autoimmune regulation, inflammatory diseases, lupus, rheumatoid arthritis). This scoping review first its kind aimed at summarizing extensive potential broad range disorders.

Language: Английский

Citations

16
Neutrophils and Asthma DOI Creative Commons

Akira Yamasaki,

Ryota Okazaki,

Tomoya Harada

et al.

Diagnostics, Journal Year: 2022, Volume and Issue: 12(5), P. 1175 - 1175

Published: May 8, 2022

Although eosinophilic inflammation is characteristic of asthma pathogenesis, neutrophilic also marked, and eosinophils neutrophils can coexist in some cases. Based on the proportion sputum cell differentiation, classified into asthma, paucigranulocytic asthma. Classification by bronchoalveolar lavage performed. Eosinophilic accounts for most severe cases, but or a mixture two types present phenotype. Biomarkers diagnosis include neutrophils, blood chitinase-3-like protein, hydrogen sulfide serum. Thymic stromal lymphoprotein (TSLP)/T-helper 17 pathways, bacterial colonization/microbiome, neutrophil extracellular traps, activation nucleotide-binding oligomerization domain-like receptor family, pyrin domain-containing 3 pathways are involved pathophysiology coexistence obesity, gastroesophageal reflux disease, habitual cigarette smoking have been associated with its pathogenesis. Thus, targeting important. Smoking cessation, neutrophil-targeting treatments, biologics tested as treatments clinical studies not focused Phosphodiesterase inhibitors, anti-TSLP antibodies, azithromycin, anti-cholinergic agents promising drugs However, research required to elucidate optimal treatment.

Language: Английский

Citations

41
Identification of extracellular vesicle microRNA signatures specifically linked to inflammatory and metabolic mechanisms in obesity‐associated low type‐2 asthma DOI Creative Commons
Fahd Alhamdan, Timm Greulich, Christian Daviaud

et al.

Allergy, Journal Year: 2023, Volume and Issue: 78(11), P. 2944 - 2958

Published: July 24, 2023

Abstract Rationale and Objective Plasma extracellular vesicles (EVs) represent a vital source of molecular information about health disease states. Due to their heterogenous cellular sources, EVs cargo may predict specific pathomechanisms behind phenotypes. Here we aimed utilize EV microRNA (miRNA) signatures gain new insights into underlying mechanisms obesity‐associated low type‐2 asthma. Methods Obese asthma (OA) non‐obese (NOA) patients were selected from an cohort conjointly with healthy controls. isolated characterised by nanoparticle tracking analysis. EV‐associated small RNAs extracted, sequenced bioinformatically analysed. Results Based on miRNA expression profiles, clear distinction between the three study groups could be established using principal component Integrative pathway analysis potential target genes differentially expressed miRNAs revealed inflammatory cytokines (e.g., interleukin‐6, transforming growth factor‐beta, interferons) metabolic factors insulin, leptin) signalling pathways specifically associated OA. The miR‐17–92 miR‐106a–363 clusters significantly enriched only in These exhibited discrete bivariate correlations several key laboratory C‐reactive protein) lung function parameters. mirrored blood‐derived CD4 + T‐cell transcriptome data, but achieved even higher sensitivity identifying affected biological pathways. Conclusion identified plasma particularly ‐106a–363 capable disentangle phenotype, which serve as basis for stratified treatment development.

Language: Английский

Citations

24
Recent Developments in the Role of Histone Acetylation in Asthma DOI
Daniel P. Potaczek, Stanisława Bazan‐Socha, Ewa Wypasek

et al.

International Archives of Allergy and Immunology, Journal Year: 2024, Volume and Issue: 185(7), P. 641 - 651

Published: Jan. 1, 2024

Epigenetic modifications are known to mediate both beneficial and unfavorable effects of environmental exposures on the development clinical course asthma. On molecular level, epigenetic mechanisms participate in multiple aspects emerging ongoing asthma pathology.

Language: Английский

Citations

10
Recent miRNA Research in Asthma DOI Creative Commons
Rinku Sharma, Anshul Tiwari, Michael J. McGeachie

et al.

Current Allergy and Asthma Reports, Journal Year: 2022, Volume and Issue: 22(12), P. 231 - 258

Published: Dec. 1, 2022

The study of microRNA in asthma has revealed a vibrant new level gene regulation underlying pathology. Several miRNAs have been shown to be important asthma, influencing various biological mechanisms which lead pathology and symptoms. In addition, proposed as biomarkers affection status, severity, treatment response. We review all recent asthma-miRNA work, while also presenting comprehensive tables miRNA results related asthma.We here reviewed 63 studies published reporting research, an additional 14 reviews the same. summarized information for both adult childhood well research on asthma-COPD overlap syndrome (ACOs), virus-induced exacerbations. attempted present collection recently asthma-associated asthma-related results.

Language: Английский

Citations

34
Extracellular Vesicles Linking Inflammation, Cancer and Thrombotic Risks DOI Creative Commons
Sarah Beck, Bernhard Hochreiter, Johannes A. Schmid

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: March 17, 2022

Extracellular vesicles (EVs) being defined as lipid-bilayer encircled particles are released by almost all known mammalian cell types and represent a heterogenous set of fragments that found in the blood circulation other body fluids. The current nomenclature distinguishes mainly three forms: microvesicles, which formed budding from plasma membrane; exosomes, released, when endosomes with intraluminal fuse apoptotic bodies representing cells. Their importance for great variety biological processes became increasingly evident last decade it was discovered they contribute to intercellular communication transferring nucleotides proteins recipient In this review, we delineate several aspects their isolation, purification, analysis; discuss some pitfalls have be considered therein. Further on, describe various cellular sources EVs explain different examples, how link cancer inflammatory conditions thrombotic processes. particular, elaborate on roles cancer-associated thrombosis COVID-19, two important paradigms, where local pathological systemic effects whole organism at least part via EVs. Finally, also possible developments field future might used biomarkers diagnosis, vehicles therapeutics.

Language: Английский

Citations

32
Effects, methods and limits of the cryopreservation on mesenchymal stem cells DOI Creative Commons
Jialing Wang, Rui Li

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 29, 2024

Language: Английский

Citations

6
Blood-Based Biomarkers for Eosinophilic Esophagitis and Concomitant Atopic Diseases: A Look into the Potential of Extracellular Vesicles DOI Open Access
Elena Grueso-Navarro, Pilar Navarro, Emilio J. Laserna‐Mendieta

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3669 - 3669

Published: Feb. 11, 2023

Eosinophilic esophagitis (EoE) is a chronic, Th2-inflammatory disease of the esophagus that can severely affect food intake. Currently, diagnosis and assessing response to treatment EoE highly invasive requires endoscopy with esophageal biopsies. Finding non-invasive accurate biomarkers important for improving patient well-being. Unfortunately, usually accompanied by other atopies, which make it difficult identify specific biomarkers. Providing an update circulating concomitant atopies therefore timely. This review summarizes current knowledge in blood two its most common comorbidities, bronchial asthma (BA) atopic dermatitis (AD), focusing on dysregulated proteins, metabolites, RNAs. It also revises extracellular vesicles (EVs) as BA AD, concludes potential use EVs EoE.

Language: Английский

Citations

16
MiRNA and Exosomal miRNA as New Biomarkers Useful to Phenotyping Severe Asthma DOI Creative Commons
Piera Soccio, Giorgia Moriondo, Donato Lacedonia

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(10), P. 1542 - 1542

Published: Oct. 18, 2023

Severe asthma (SA) is a chronic inflammatory disease of the airways. Due to extreme heterogeneity symptoms, new biomarkers are currently needed. MiRNAs non-coding RNAs that negatively regulate gene expression at post-transcriptional level. In biological fluids, miRNAs contained within exosomes, vesicles capable giving considerable stability and resistance degradation by RNAses. The main function attributed exosomes intercellular communication. goal our study was analyze intracellular exosomal in order demonstrate their potential use as non-invasive showing, particular, role SA. We detected qRT-PCR both serum serum-derived-exosomes asthmatic patients healthy controls. levels almost all analyzed (miR-21, miR-223, let-7a) were greater vs. control, except for miR-223. detail, miR-21 SA, while let-7a increased mild-to-moderate asthma. On other hand, higher This identified series involved highlighting development progression. These results need validation on larger cohort.

Language: Английский

Citations

15