Induced pluripotent stem cell-derived mesenchymal stem cells for modeling and treating metabolic associated fatty liver disease and metabolic associated steatohepatitis: Challenges and opportunities DOI
Bárbara M. Silva, José Bragança

World Journal of Stem Cells, Journal Year: 2025, Volume and Issue: 17(2)

Published: Feb. 24, 2025

The potential of induced pluripotent stem cells (iPSCs) for modeling and treating metabolic associated fatty liver disease (MAFLD) steatohepatitis (MASH) is emerging. MAFLD a growing global health concern, currently with limited treatment options. While primary mesenchymal hold promise, iPSCs offer versatile alternative due to their ability differentiate into various cell types, including iPSC-derived cells. However, challenges remain, optimizing differentiation protocols, ensuring safety, addressing tumorigenicity risks. In addition, the possibility generate complex cellular models, three-dimensional organoid which are closer representations human than animal models. Those models would also be valuable drug discovery personalized medicine approaches. Overall, derivatives new perspectives advancing MAFLD/MASH research developing novel therapeutic strategies. Further needed overcome current limitations translate this effective clinical applications.

Language: Английский

Mesenchymal stem cells in the treatment of spinal cord injury: Mechanisms, current advances and future challenges DOI Creative Commons

Yuanliang Xia,

Jianshu Zhu,

Ruohan Yang

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 24, 2023

Spinal cord injury (SCI) has considerable impact on patient physical, mental, and financial health. Secondary SCI is associated with inflammation, vascular destruction, subsequent permanent damage to the nervous system. Mesenchymal stem cells (MSCs) have anti-inflammatory properties, promoting regeneration release neuro-nutrients, are a promising strategy for treatment of SCI. Preclinical studies shown that MSCs promote sensory motor function recovery in rats. In clinical trials, been reported improve American Injury Association (ASIA) scores. However, effectiveness treating patients remains controversial. tumorigenesis ensuring survival hostile environment challenging. this article we examine evidence pathophysiological changes occurring after We then review underlying mechanisms summarize potential application practice. Finally, highlight challenges surrounding use discuss future applications.

Language: Английский

Citations

54
Understanding exosomes: Part 2—Emerging leaders in regenerative medicine DOI Creative Commons
Richard J. Miron, Nathan E. Estrin, Anton Sculean

et al.

Periodontology 2000, Journal Year: 2024, Volume and Issue: 94(1), P. 257 - 414

Published: Feb. 1, 2024

Abstract Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with ability to communicate other tissues and cell types over long distances. Their use in regenerative medicine has gained tremendous momentum recently due their be utilized as therapeutic options for a wide array diseases/conditions. Over 5000 publications currently being published yearly on this topic, number is only expected dramatically increase novel strategies continue developed. Today exosomes have been applied numerous contexts including neurodegenerative disorders (Alzheimer's disease, central nervous system, depression, multiple sclerosis, Parkinson's post‐traumatic stress disorders, traumatic brain injury, peripheral nerve injury), damaged organs (heart, kidney, liver, stroke, myocardial infarctions, ovaries), degenerative processes (atherosclerosis, diabetes, hematology musculoskeletal degeneration, osteoradionecrosis, respiratory disease), infectious diseases (COVID‐19, hepatitis), procedures (antiaging, bone regeneration, cartilage/joint osteoarthritis, cutaneous wounds, dental dermatology/skin erectile dysfunction, hair regrowth, intervertebral disc repair, spinal cord vascular regeneration), cancer therapy (breast, colorectal, gastric osteosarcomas), immune function (allergy, autoimmune regulation, inflammatory diseases, lupus, rheumatoid arthritis). This scoping review first its kind aimed at summarizing extensive potential broad range disorders.

Language: Английский

Citations

19
Extracellular Vesicles: The Next Generation Theranostic Nanomedicine for Inflammatory Bowel Disease DOI Creative Commons
Defeng Li, Meifeng Yang, Jing Xu

et al.

International Journal of Nanomedicine, Journal Year: 2022, Volume and Issue: Volume 17, P. 3893 - 3911

Published: Sept. 1, 2022

Abstract: The recent rapid development in the field of extracellular vesicles (EVs) based nanotechnology has provided unprecedented opportunities for nanomedicine platforms. As natural nanocarriers, EVs such as exosomes, exosome-like nanoparticles and outer membrane (OMVs), have unique structure/composition/morphology characteristics, show excellent physical chemical/biochemical properties, making them a new generation theranostic nanomedicine. Here, we reviewed characteristics from perspective their formation biological function inflammatory bowel disease (IBD). Moreover, can crucially participate interaction communication intestinal epithelial cells (IECs)-immune cells-gut microbiota to regulate immune response, inflammation homeostasis. Interestingly, on current representative examples exosomes IBD treatment, it is shown that plant, milk, cells-derived exert therapeutic effect through components, proteins, nucleic acid, lipids. several drug loading methods target modification are used improve capability. We also discussed application treatment IBD. In this review, aim better more clearly clarify underlying mechanisms pathogenesis IBD, provide directions mediated treatment. Keywords: vesicles, exosome, nanoparticles, disease,

Language: Английский

Citations

42
Exosome-loaded degradable polymeric microcapsules for the treatment of vitreoretinal diseases DOI
Han Bao, Ying Tian, Haixin Wang

et al.

Nature Biomedical Engineering, Journal Year: 2023, Volume and Issue: 8(11), P. 1436 - 1452

Published: Oct. 23, 2023

Language: Английский

Citations

34
Understanding molecular characteristics of extracellular vesicles derived from different types of mesenchymal stem cells for therapeutic translation DOI Creative Commons
Zuo Ding, Zachary Greenberg,

Maria Fernanda Serafim

et al.

Extracellular Vesicle, Journal Year: 2024, Volume and Issue: 3, P. 100034 - 100034

Published: March 2, 2024

Mesenchymal stem cells (MSCs) have been studied for decades as candidates cellular therapy, and their secretome, including secreted extracellular vesicles (EVs), has identified to contribute significantly regenerative reparative functions. Emerging evidence suggested that MSC-EVs alone, could be used therapeutics emulate the biological function of MSCs. However, just with MSCs, shown vary in composition, depending on tissue source MSCs well protocols employed culturing obtaining EVs. Therefore, importance careful choice cell sources culture environments is receiving increasing attention. Many factors therapeutic potential MSC-EVs, tissue, isolation technique, conditions. This review illustrates molecular landscape EVs derived from different types MSC along strategies. A thorough analysis publicly available omic datasets was performed advance precision understanding unique source-dependent characteristics. The tissue-specific protein miRNA-driven Reactome ontology reveal distinct patterns top pathways across adipose, bone marrow, umbilical MSC-EVs. Moreover, a meta-analysis assisted by an AI technique analyze published literature, providing insights into translation based tissues.

Language: Английский

Citations

13
MiR-124 Delivered by Extracellular Vesicles from Mesenchymal Stem Cell Exerts Neuroprotective Effects by Stabilizing the p62-Keap1-Nrf2 Pathway after Spinal Cord Injury in Rats DOI Creative Commons
Chao Fang, Jun Qian, Bizhi Tu

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

Spinal cord injury (SCI) can cause irreversible trauma to nervous tissue, leading permanent damage the patient's motor and sensory functions. Extracellular vesicles derived from mesenchymal stem cells (MSC-EVs) simulate most of functions MSCs are considered an ideal treatment option for SCI. However, potential mechanism MSC-EVs SCI still needs be explored. We cultured neurons in vitro investigate effect miR-124 on p62-Keap1-Nrf2 pathway. Besides, containing were injected into a rat spinal model observe their neural repair effect. The accumulation p62 reversed by miR-124, which promotes autophagy alleviates oxidative stress, thereby exerting neuroprotective effects. Rats who received injection overexpressing after surgery showed higher BBB scores, lower levels cell apoptosis, better tissue morphology. Our results indicated that stabilize loop, promoting alleviating stress exert research proposes novel target treating

Language: Английский

Citations

1
Effects of astrocytes and microglia on neuroinflammation after spinal cord injury and related immunomodulatory strategies DOI

Qi-Ming Pang,

Siyu Chen,

Qi-Jing Xu

et al.

International Immunopharmacology, Journal Year: 2022, Volume and Issue: 108, P. 108754 - 108754

Published: April 6, 2022

Language: Английский

Citations

38
Bone marrow mesenchymal stem cells and exercise restore motor function following spinal cord injury by activating PI3K/AKT/mTOR pathway DOI Creative Commons
Wei Quan, Xin Sun, Liyi Huang

et al.

Neural Regeneration Research, Journal Year: 2022, Volume and Issue: 18(5), P. 1067 - 1067

Published: Oct. 27, 2022

Although many therapeutic interventions have shown promise in treating spinal cord injury, focusing on a single aspect of repair cannot achieve successful and functional regeneration patients following injury . In this study, we applied combinatorial approach for involving neuroprotection rehabilitation, exploiting cell transplantation sensorimotor training to promote nerve recovery. Here, used mouse model thoracic contusive investigate whether the combination bone marrow mesenchymal stem exercise has synergistic effect restoration. Locomotor function was evaluated by Basso Mouse Scale, horizontal ladder test, footprint analysis. Magnetic resonance imaging, histological examination, transmission electron microscopy observation, immunofluorescence staining, western blotting were performed 8 weeks after further explore potential mechanism behind effect. vivo, showed better motor than treatments. Further investigations revealed that markedly reduced fibrotic scar tissue, protected neurons, promoted axon myelin protection. Additionally, effects recovery occurred via PI3K/AKT/mTOR pathway. vitro, experimental evidence from PC12 line primary cortical neuron culture also demonstrated blocking pathway would aggravate neuronal damage. Thus, combined with can effectively restore activating

Language: Английский

Citations

36
Berberine-loaded MSC-derived sEVs encapsulated in injectable GelMA hydrogel for spinal cord injury repair DOI
He Zhang, Qin Tang, Yang Lu

et al.

International Journal of Pharmaceutics, Journal Year: 2023, Volume and Issue: 643, P. 123283 - 123283

Published: Aug. 1, 2023

Language: Английский

Citations

20
Exosomes combined with biomaterials in the treatment of spinal cord injury DOI Creative Commons
Xuanxuan Zhang,

Wenwei Jiang,

Yan Lü

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: March 13, 2023

Spinal cord injury (SCI) is a serious and disabling disease with high mortality rate. It often leads to complete or partial sensory motor dysfunction accompanied by series of secondary outcomes, such as pressure sores, pulmonary infections, deep vein thrombosis in the lower extremities, urinary tract autonomic dysfunction. Currently, main treatments for SCI include surgical decompression, drug therapy, postoperative rehabilitation. Studies have shown that cell therapy plays beneficial role treatment SCI. Nonetheless, there controversy regarding therapeutic effect transplantation models. Meanwhile exosomes, new medium regenerative medicine, possess advantages small size, low immunogenicity, ability cross blood-spinal barrier. Certain studies stem cell-derived exosomes anti-inflammatory effects can play an irreplaceable In this case, it difficult single method effective repair neural tissue after The combination biomaterial scaffolds better transfer fix site improve their survival This paper first reviews current research status respectively, then describes application combined SCI, well challenges prospects.

Language: Английский

Citations

19