Enhanced hemostatic efficacy of cryogel with copper ion-loaded mesoporous bioactive glasses for acute and persistent bleeding DOI Creative Commons
Qixiu Hou, Xu He,

Mengting Guo

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 12, 2025

Uncontrolled acute and persistent bleeding, as well with infection, is a great challenge because of the high mortality during treating patients injuries, complex surgery or bone marrow failure. Here, we develop an external form natural components which based on phosphorylated methacrylated gelatin (GelMA, G) cryogel (GP) loaded tannic acid (TA)-mixed copper ion (Cu2+) mesoporous bioactive glasses (MBG), named after GP@MBG-Cu-TA cryogel, to address goals reduce bleeding enhance antibacterial activity. Structurally, GP, MBG TA Cu2+ adheres GP via hydrogen bonding. In vitro, displays good biocompatibility, hemostatic antimicrobial capability. vivo studies, can effect in liver injury SD rats for aplastic anemia hemophilia A mice tail amputation bleeding. addition, accelerates skin wound repair bacterial contamination at site. sum, not only endowed dual function capability, but also stop objects either normal abnormal coagulation function. Thus, provides promising candidate dressing managing complications clinic.

Language: Английский

Gastric cancer-derived exosomal miR-519a-3p promotes liver metastasis by inducing intrahepatic M2-like macrophage-mediated angiogenesis DOI Creative Commons
Shengkui Qiu,

Li Xie,

Lu Chen

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2022, Volume and Issue: 41(1)

Published: Oct. 10, 2022

Liver metastasis (LM) is a major obstacle to the prognosis of gastric cancer (GC) patients, but molecular mechanism underlying liver (GC-LM) remains unknown. Exosomes have been identified as an important mediator communication between tumor cells and microenvironment. Therefore, we sought investigate effects primary GC on microenvironment role exosomal microRNAs (exo-miRNA) in GC-LM.Sequential differential centrifugation, transmission electron microscopy NanoSight analysis were used extract characterize exosomes. MicroRNA sequencing GC-derived exosomes mRNA PMA-treated THP-1 identify differentially expressed miRNAs functional targets miR-519a-3p (exo-miR-519a-3p) macrophages, respectively. Tracing internalization transfer exo-miR-519a-3p observed by immunofluorescence. Tubule formation assays, aortic ring exosome-educated GC-LM model roles angiogenesis GC-LM. Luciferase reporter assay, qRT-PCR, Western blot, ELISA, flow cytometry immunofluorescence regulatory at GC-LM.The expression level serum was significantly higher patients than without LM, high indicates worse prognosis. are mainly accumulated internalized intrahepatic macrophages. Mechanistically, activates MAPK/ERK pathway targeting DUSP2, thereby causing M2-like polarization polarized macrophages accelerate inducing promoting premetastatic niche formation.Our results indicate that plays critical mediating crosstalk potential therapeutic target for

Language: Английский

Citations

130
The role of macrophages polarization in sepsis-induced acute lung injury DOI Creative Commons
Ziyi Wang,

Zhong Wang

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 24, 2023

Sepsis presents as a severe infectious disease frequently documented in clinical settings. Characterized by its systemic inflammatory response syndrome, sepsis has the potential to trigger multi-organ dysfunction and can escalate becoming life-threatening. A common fallout from is acute lung injury (ALI), which often progresses respiratory distress syndrome (ARDS). Macrophages, due their significant role immune system, are receiving increased attention studies. Macrophage polarization process that hinges on an intricate regulatory network influenced myriad of signaling molecules, transcription factors, epigenetic modifications, metabolic reprogramming. In this review, our primary focus classically activated macrophages (M1-like) alternatively (M2-like) two paramount phenotypes instrumental sepsis’ host response. An imbalance between M1-like M2-like precipitate onset exacerbate progression sepsis. This review provides comprehensive understanding interplay macrophage sepsis-induced (SALI) elaborates intervention strategy centers around crucial polarization.

Language: Английский

Citations

74
Cancer chemotherapy resistance: Mechanisms and recent breakthrough in targeted drug delivery DOI
Fatemeh Davodabadi, Seyedeh Fatemeh Sajjadi, Mohammad Sarhadi

et al.

European Journal of Pharmacology, Journal Year: 2023, Volume and Issue: 958, P. 176013 - 176013

Published: Aug. 24, 2023

Language: Английский

Citations

57
Macrophage polarization in inflammatory bowel disease DOI Creative Commons
Kun Zhang, Jing Guo,

Wenlong Yan

et al.

Cell Communication and Signaling, Journal Year: 2023, Volume and Issue: 21(1)

Published: Dec. 21, 2023

Abstract The growing prevalence of inflammatory bowel disease (IBD) has encouraged research efforts, which have contributed to gradual improvements in our understanding IBD diagnosis and therapeutic approaches. pathogenesis not been fully elucidated; however, the combined actions environmental, genetic, immune factors, microbial organisms are believed cause IBD. In innate system, macrophages play important roles maintaining intestinal health development Macrophages can be polarized from M0 into several phenotypes, among M1 M2 critical repair homeostasis damage. Certain macrophage-related studies already exist; functions each phenotype elucidated. As technology develops, link between increased, including knowledge developmental origins their performance comprehensive functions. This review describes macrophage polarization perspectives polarization, changes IBD, metabolic changes, mechanisms discussion these topics provides new insights immunotherapy strategies for

Language: Английский

Citations

49
Signaling pathways in macrophages: molecular mechanisms and therapeutic targets DOI Creative Commons
Ming Li, Mengjie Wang, Yuanjia Wen

et al.

MedComm, Journal Year: 2023, Volume and Issue: 4(5)

Published: Sept. 11, 2023

Abstract Macrophages play diverse roles in development, homeostasis, and immunity. Accordingly, the dysfunction of macrophages is involved occurrence progression various diseases, such as coronavirus disease 2019 atherosclerosis. The protective or pathogenic effect that exert different conditions largely depends on their functional plasticity, which regulated via signal transduction Janus kinase–signal transducer activator transcription, Wnt Notch pathways, stimulated by environmental cues. Over past few decades, molecular mechanisms signaling pathways have been gradually elucidated, providing more alternative therapeutic targets for diseases treatment. Here, we provide an overview basic physiology expound regulatory within them. We also address crucial role pathogenesis including autoimmune, neurodegenerative, metabolic, infectious cancer, with a focus advances macrophage‐targeted strategies exploring modulation components regulators pathways. Last, discuss challenges possible solutions therapy clinical applications. hope this comprehensive review will directions further research targeting macrophage are promising to improve efficacy

Language: Английский

Citations

46
Macrophage in liver Fibrosis: Identities and mechanisms DOI Creative Commons
Zhi Wang, Kailei Du,

Nake Jin

et al.

International Immunopharmacology, Journal Year: 2023, Volume and Issue: 120, P. 110357 - 110357

Published: May 22, 2023

Liver fibrosis is a chronic disease characterized by the deposition of extracellular matrix and continuous loss tissues that perform liver functions. Macrophages are crucial modulators innate immunity play important roles in fibrogenesis. comprise heterogeneous subpopulations exhibit different cellular Understanding identity function these cells essential for understanding mechanisms According to definitions, macrophages divided into M1/M2 or monocyte-derived macrophages/Kupffer cells. Classic phenotyping corresponds pro- anti-inflammatory effects, and, therefore, influences degree later phases. In contrast, origin closely associated with their replenishment activation during fibrosis. These two classifications depict dynamics liver-infiltrating macrophages. However, neither description properly elucidates positive negative role Critical tissue mediating include hepatic stellate fibroblasts, being particular interest because close association molecular biological descriptions inconsistent between mice humans, warranting further investigations. fibrosis, can secrete various pro-fibrotic cytokines, such as TGF-β, Galectin-3 interleukins (ILs), fibrosis-inhibiting IL10. secretions may be specific spatiotemporal characteristics Furthermore, dissipation, degrade secreting metalloproteinases (MMPs). Notably, using therapeutic targets has been explored. The current approaches categorized follows: treatment macrophage-related molecules macrophage infusion therapy. Although there have limited studies, shown reliable potential treatment. this review, we focu on relationship progression regression

Language: Английский

Citations

45
Recent Advances in CAR-Based Solid Tumor Immunotherapy DOI Creative Commons
Min Hwa Shin,

Eunha Oh,

Yun Jeong Kim

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(12), P. 1606 - 1606

Published: June 11, 2023

Adoptive cell therapy using chimeric antigen receptor (CAR) technology is one of the most advanced engineering platforms for cancer immunotherapy. CAR-T cells have shown remarkable efficacy in treatment hematological malignancies. However, their limitations solid tumors include an immunosuppressive tumor microenvironment (TME), insufficient infiltration, toxicity, and absence tumor-specific antigens. Although recent advances design-such as incorporation co-stimulatory domains development armored cells-have promising results treating tumors, there are still challenges that need to be addressed. To overcome these limitations, other immune cells, such natural killer (NK) macrophages (M), been developed attractive options efficient immunotherapy tumors. CAR-NK exhibit substantial clinical improvements with "off-the-shelf" availability low toxicity. CAR-M therapeutic potential because can infiltrate TME Here, we review future perspectives associated engineered cell-based immunotherapies We also summarize ongoing trials investigating safety CAR-T, CAR-NK, CAR-M, targeting

Language: Английский

Citations

43
Layer-by-layer self-assembly coatings on strontium titanate nanotubes with antimicrobial and anti-inflammatory properties to prevent implant-related infections DOI

Jiaxin Guan,

Jiali Wang,

Fengzhen Jia

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2024, Volume and Issue: 244, P. 114183 - 114183

Published: Aug. 28, 2024

Language: Английский

Citations

30
Dopamine-mediated copper-loaded ZnTiO3 antimicrobial coating with immunomodulatory properties effectively enhances vascularised osteogenesis on titanium implants DOI
Anqi Bian, Yuxuan Sun,

Jiaxin Guan

et al.

Journal of Industrial and Engineering Chemistry, Journal Year: 2024, Volume and Issue: 135, P. 94 - 109

Published: Jan. 10, 2024

Language: Английский

Citations

21
The Role of Microglia in Brain Metastases: Mechanisms and Strategies DOI Creative Commons
Ying Feng, Xueqing Hu, Yingru Zhang

et al.

Aging and Disease, Journal Year: 2024, Volume and Issue: 15(1), P. 169 - 169

Published: Jan. 1, 2024

Brain metastases and related complications are one of the major fatal factors in cancer. Patients with breast cancer, lung melanoma at a high risk developing brain metastases. However, mechanisms underlying metastatic cascade remain poorly understood. Microglia, resident macrophages parenchyma, involved multiple processes associated metastasis, including inflammation, angiogenesis, immune modulation. They also closely interact cancer cells, astrocytes, other cells. Current therapeutic approaches against cancers, small-molecule drugs, antibody-coupled drugs (ADCs), immune-checkpoint inhibitors (ICIs), have compromised efficacy owing to impermeability blood-brain barrier (BBB) complex microenvironment. Targeting microglia is strategies for treating In this review, we summarize multifaceted roles highlight them as potential targets future interventions.

Language: Английский

Citations

17