The Challenge of Applications of Probiotics in Gastrointestinal Diseases

Advanced Gut & Microbiome Research, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 10

Published: Jan. 18, 2023

Gastrointestinal disease is characterized by gastrointestinal dysfunction with dysbiosis of the microbiome. Probiotics may act as biological agents in treating diseases through modifying gut microbiota. However, several challenges, including safety, stress resistance, postcolonization quantification, and evaluation models, hinder application probiotics diseases. This review introduces emerging methods for delivering well available materials. Furthermore, we elucidated bacteriocins their role helping obtain a competitive advantage over other strains challenges large-scale application. Bacteriocins produced also showed promising efficacy capacity immune stimulation, intestinal barrier protection, cytotoxicity against tumorigenesis. For quantification complex microbiomes probiotic encapsulated delivery systems, recent fluorescent labeling technology various vitro vivo models were reviewed. Given widespread use microecological therapy diseases, further understanding multiple updated to improve colonization system great significance live biotherapeutics.

Language: Английский

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Production of prebiotic xylooligosaccharides from industrial-derived xylan residue by organic acid treatment

Carbohydrate Polymers, Journal Year: 2022, Volume and Issue: 292, P. 119641 - 119641

Published: May 21, 2022

Language: Английский

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Evaluating the In Vitro and In Vivo Prebiotic Effects of Different Xylo-Oligosaccharides Obtained from Bamboo Shoots by Hydrothermal Pretreatment Combined with Endo-Xylanase Hydrolysis

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(17), P. 13422 - 13422

Published: Aug. 30, 2023

Xylo-oligosaccharides (XOS) enriched with high fractions of X2-X3 are regarded as an effective prebiotic for regulating the intestinal microflora. In this study, original XOS solution was obtained from bamboo shoots through hydrothermal pretreatment under optimized conditions. Subsequently, enzymatic hydrolysis endo-xylanase performed on to enhance abundance fractions. The results demonstrated that yielded 21.24% in hydrolysate solution, and subsequent significantly increased proportion 38.87% 68.21%. Moreover, solutions higher amounts exhibited superior performance promoting growth probiotics such Bifidobacterium adolescentis Lactobacillus acidophilus vitro, leading production short-chain fatty acids. vivo colitis mouse model, contents enhanced efficacy against inflammation. Compared mice (model group), (S1 group) could increase number Streptomyces microflora, while (S2 Bacteroides microflora mice. addition, abundances Alcaligenes Pasteurella S1 S2 groups were much lower than model group. This effect attributed ability these species diversity, reversing imbalance disorder within Overall, work highlights outstanding potential a regulator microbiota anti-colitis agent.

Language: Английский

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The effects of live and pasteurized Akkermansia muciniphila on DSS-induced ulcerative colitis, gut microbiota, and metabolomics in mice

Food & Function, Journal Year: 2023, Volume and Issue: 14(10), P. 4632 - 4646

Published: Jan. 1, 2023

Akkermansia muciniphila (A. muciniphila) plays an important role in intestinal regulation, while it is presently unclear whether live or pasteurized A. has different effects on the health. In present study, was administered to dextran sulfate sodium (DSS)-induced ulcerative colitis mice investigate its influences host health, gut microbiota, and metabolomic phenotype. The results showed that alleviated symptoms of more efficiently by encouraging proliferation beneficial bacteria, increasing production short-chain fatty acids, inhibiting inflammation. Moreover, increased abundance Parasutterella thus affected metabolism lipid lipid-like molecules associated with lysophosphatidylcholines (LysoPCs). Notably, prophylactic supplementation relative anti-inflammatory microbe Dubosiella, thereby activating sphingolipid alleviate damage. conclusion, a effective amelioration DSS-induced repairing dysbiosis microbiota compared muciniphila, providing potential strategy explore protective effect

Language: Английский

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Progress on the Anti-Inflammatory Activity and Structure–Efficacy Relationship of Polysaccharides from Medical and Edible Homologous Traditional Chinese Medicines

Molecules, Journal Year: 2024, Volume and Issue: 29(16), P. 3852 - 3852

Published: Aug. 14, 2024

Medicinal food varieties developed according to the theory of medical and edible homologues are effective at preventing treating chronic diseases in health care. As 2022, 110 types traditional Chinese medicines from same source medicine have been published by National Health Commission. Inflammation is immune system’s first response injury, infection, stress. Chronic inflammation closely related many such as atherosclerosis cancer. Therefore, timely intervention for mainstay treatment other complex diseases. However, some anti-inflammatory drugs on market commonly associated with a number adverse effects, which seriously affect safety patients. in-depth development new safe, harmless, has become hot topic research an urgent clinical need. Polysaccharides, one main active ingredients homologous (MEHTCMs), confirmed large studies exert effects through multiple targets considered potential natural drugs. In addition, structure medicines’ polysaccharides (MEHTCMPs) may be key factor determining their activity, makes underlying structure–efficacy relationship topics domestic international research. due limitations current analytical techniques tools, structures not fully elucidated relatively ambiguous, difficulties process developing utilizing MEHTCMPs novel future. For this reason, paper summarizes mechanisms MEHTCMPs, regulation Toll-like receptor-related signaling pathway, MAPK JAK-STAT NLRP3 PI3K-AKT PPAR-γ Nrf2-HO-1 intestinal flora, it systematically analyzes evaluates relationships between activity structures.

Language: Английский

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Metabolic engineering of Lactobacilli spp. for disease treatment

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 6, 2025

A variety of probiotics have been utilized as chassis strains and engineered to develop the synthetic for disease treatment. Among these probiotics, Lactobacilli, which are generally viewed safe capable colonizing gastrointestinal tract effectively, widely used. We review recent advancements in engineering Lactobacilli Specifically, that used construction application diseases treatment, therapeutic outcomes microbes summarized this review. Moreover, applications treatment categorized based on their strategies. Of note, we compare advantages disadvantages various strategies offer insights future development genetically modified Lactobacillus with stable properties. Our study comprehensively reviews researches diverse by strategies, emphasizes importance developing characteristics enhance applications.

Language: Английский

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Clostridium butyricum and Chitooligosaccharides in Synbiotic Combination Ameliorate Symptoms in a DSS-Induced Ulcerative Colitis Mouse Model by Modulating Gut Microbiota and Enhancing Intestinal Barrier Function

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)

Published: March 28, 2023

Effects of Clostridium butyricum and chitooligosaccharides (COS), singly in synbiotic combination, were evaluated a C57BL/6 mouse model dextran sulfate (DSS)-induced acute ulcerative colitis (UC). Treatment with C. and/or COS ameliorated UC symptoms vivo, the strongest effects observed for combination terms reduced mortality rates disease activity indices, increased body weight colon length, improved histological features. The achieved following: (i) regulated levels inflammation-related cytokines (tumor necrosis factor alpha [TNF-α], interleukin-1β [IL-1β], IL-6, IL-10) had stronger anti-inflammatory effect than either component alone, based on inhibition Toll-like receptor 4 (TLR-4)/NF-κB/MAPK signaling pathway activation; (ii) enhanced intestinal barrier function by restoring tight junction proteins (occludin, claudin-1, ZO-1) MUC2; (iii) abundance diversity beneficial bacteria (gut microbiota) pathogenic bacteria; (iv) production short-chain fatty acids. Our findings indicate that has strong potential as therapeutic adjuvant UC. IMPORTANCE Ulcerative (UC), an idiopathic characterized continuous remission/relapse inflammatory cycles colonic mucosal layer, adverse patients' quality life considerable costs health care systems. Probiotics, prebiotics, synbiotics are regarded agents UC, safety efficacy. In this study, we present detailed evaluation DSS-induced composed (molecular [MW], 2,500 Da). We found synergistic (synbiotic) action is more effective alone prevention therapy regulating gut microbiota function. development anti-UC drugs or pharmaceutical, food, livestock industries. Highlights include following. clinical morphology. displayed antioxidant effects. expression proteins. inhibited TRL-4/NF-κB/MAPK pathway. (v) modulated composition.

Language: Английский

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Development of probiotic E. coli Nissle 1917 for β-alanine production by using protein and metabolic engineering

Applied Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 107(7-8), P. 2277 - 2288

Published: March 16, 2023

Language: Английский

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Targeting gut microbiota and immune crosstalk: potential mechanisms of natural products in the treatment of atherosclerosis

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 1, 2023

Atherosclerosis (AS) is a chronic inflammatory reaction that primarily affects large and medium-sized arteries. It major cause of cardiovascular disease peripheral arterial occlusive disease. The pathogenesis AS involves specific structural functional alterations in various populations vascular cells at different stages the immune response involved throughout entire developmental stage AS, targeting presents promising avenue for its treatment. Over past 2 decades, studies have shown gut microbiota (GM) metabolites, such as trimethylamine-N-oxide, significant impact on progression AS. Interestingly, it has also been reported there are complex mechanisms action between GM their responses, natural products can an metabolites regulate expression potential impacts Natural wide range health properties, researchers increasingly focusing role Now, compelling evidence provide alternative approach to improving function microenvironment by modulating GM. product resveratrol, berberine, curcumin, quercetin may improve intestinal relative abundance GM, which turn influences accumulation metabolites. delay regulating metabolism inhibiting migration monocytes macrophages, promoting polarization M2 phenotype down-regulating level factors, balance Treg/Th17, formation foam cells. Based above, we describe recent advances use target crosstalk treat bring some insights guide treatment

Language: Английский

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Supplemented Clostridium butyricum and Bacillus subtilis affects the physiology and production performance of Yangzhou geese

Italian Journal of Animal Science, Journal Year: 2025, Volume and Issue: 24(1), P. 800 - 810

Published: March 4, 2025

Language: Английский

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