Phocaeicola vulgatus alleviates diet-induced metabolic dysfunction-associated steatotic liver disease progression by downregulating histone acetylation level via 3-HPAA

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: Feb. 5, 2024

Diet-induced metabolic dysfunction-associated steatotic liver disease (MASLD) is a prevalent disorder with limited effective interventions available. A novel approach to address this issue through gut microbiota-based therapy. In our study, we utilized multi-omics analysis identify Phocaeicola vulgatus (P. vulgatus) as potential probiotic for the treatment of MASLD. Our findings from murine models clearly illustrate that supplementation P. mitigates development This beneficial effect partly attributed metabolite 3-Hydroxyphenylacetic acid (3-HPAA) produced by vulgatus, which reduces acetylation levels H3K27 and downregulates transcription Squalene Epoxidase (SQLE), rate-limiting enzyme in steroid biosynthesis promotes lipid accumulation cells. study underscores significant role MASLD critical importance its 3-HPAA regulating homeostasis. These offer promising avenue early intervention therapy context

Language: Английский

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Moderate Wine Consumption and Health: A Narrative Review

Nutrients, Journal Year: 2022, Volume and Issue: 15(1), P. 175 - 175

Published: Dec. 30, 2022

Although it is clearly established that the abuse of alcohol seriously harmful to health, much epidemiological and clinical evidence seem underline protective role moderate quantities in particular wine on health. This narrative review aims re-evaluate relationship between type dose alcoholic drink reduced or increased risk various diseases, light most current scientific evidence. In particular, vitro studies modulation biochemical pathways gene expression bioactive components were evaluated. Twenty-four selected after PubMed, Scopus Google Scholar searches for evaluation alcohol/wine consumption health effects: eight concerned cardiovascular three 2 diabetes, four neurodegenerative five cancer related longevity. A brief discussion viticultural enological practices potentially affecting content included. The analysis indicates differs from other beverages its not only does increase chronic degenerative diseases but also associated with benefits particularly when included a Mediterranean diet model. Obviously, every effort must be made promote behavioral education prevent abuse, especially among young people.

Language: Английский

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Advances in Ferroptosis-Inducing Agents by Targeted Delivery System in Cancer Therapy

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 2091 - 2112

Published: March 1, 2024

Abstract: Currently, cancer remains one of the most significant threats to human health. Treatment cancers challenging, despite implementation diverse therapies in clinical practice. In recent years, research on mechanism ferroptosis has presented novel perspectives for treatment. Ferroptosis is a regulated cell death process caused by lipid peroxidation membrane unsaturated fatty acids catalyzed iron ions. The rapid development bio-nanotechnology generated considerable interest exploiting iron-induced as new therapeutic target against cancer. This article provides comprehensive overview advancements at intersection and bionanotechnology. this respect, its relation are summarized. Furthermore, feasibility nano-drug delivery system based treatment introduced analyzed. Secondly, strategies inducing using nanodrug technology discussed, including promoting Fenton reactions, inhibiting glutathione peroxidase 4, reducing low levels, Xc − . Additionally, explores potential combined involving Finally, application prospects challenges nanoagents discussed. Keywords: cancers, ferroptosis, system,

Language: Английский

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Prevention of Metabolic Syndrome by Phytochemicals and Vitamin D

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2627 - 2627

Published: Jan. 30, 2023

In recent years, attention has focused on the roles of phytochemicals in fruits and vegetables maintaining improving intestinal environment preventing metabolic syndrome. A high-fat high-sugar diet, lack exercise, excess energy accumulation body can cause syndrome induce obesity, diabetes, disorders circulatory system liver. Therefore, prevention is important. The current review shows that simultaneous intake contained citruses grapes together with vitamin D improves state gut microbiota immunity, related diseases. Phytochemicals include polyphenols such as hesperidin, rutin, naringin; those quercetin, procyanidin, oleanolic acid. these D, along prebiotics probiotics, nurture good microbiota. general, Firmicutes are obese-prone Bacteroidetes lean-prone microbiota; regulatory T cells, which suppress inflammatory responses upregulate immunity. Maintaining suppresses TNF-α, an cytokine also considered to be a pathogenic contributor adipokine, prevents chronic inflammation, thereby helping prevent enhances adiponectin, protector adipokine For reduction various disease risks, will important for human health future.

Language: Английский

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The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: Sept. 27, 2023

Aging-related fertility decline is a prevalent concern globally. Male reproductive system aging mainly characterized by decrease in sperm quality and fertility. While it known that intestinal physiology changes with age microbiota shaped physiology, the underlying mechanism of how affects male still largely unexplored.Here, we utilized fecal transplantation (FMT) to exchange between young old mice. Cecal shotgun metagenomics metabolomics were used identify differences gut composition metabolic regulation during aging. Our results demonstrated FMT from mice alleviated aging-associated spermatogenic dysfunction through an unexpected mediated bacteria-derived metabolite, 3-hydroxyphenylacetic acid (3-HPAA). 3-HPAA treatment resulted improvement spermatogenesis RNA sequencing analysis, qRT-PCR Western blot revealed induced upregulation GPX4, thereby restraining ferroptosis restoring spermatogenesis. These findings further confirmed vitro induction inhibition GPX4 expression.Our demonstrate microbiome-derived 3-HPAA, facilitates ferroptosis-mediated mechanism. Overall, these provide novel dysregulated mice, suggest could be potential therapy for males clinical practice. Video Abstract.

Language: Английский

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Aromatic oil from lavender as an atopic dermatitis suppressant

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(1), P. e0296408 - e0296408

Published: Jan. 5, 2024

In atopic dermatitis (AD), nerves are abnormally stretched near the surface of skin, making it sensitive to itching. Expression neurotrophic factor Artemin (ARTN) involved in such nerve stretching is induced by xenobiotic response (XRE) air pollutants and UV radiation products. Therefore, AD can be monitored XRE response. Previously, we established a human keratinocyte cell line stably expressing NanoLuc reporter gene downstream XRE. We found that 6-formylindolo[3,2-b]carbazole (FICZ), tryptophan metabolite known inducer XRE, increased mRNA expression, indicating FICZ-treated cells could model for AD. Lavender essential oil has been used folk medicine treat AD, but scientific basis its use unclear. present study, investigated efficacy lavender major components, linalyl acetate linalool, suppress sensitize skin using line, dendritic activation assays. Our results indicated from L . angustifolia exerted strong inhibitory effect almost no sensitization. useful circumvent practice animal studies evaluate medicines.

Language: Английский

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Waste to Wealth: Dynamics and metabolic profiles of the conversion of jackfruit flake into value-added products by different fermentation methods

Food Chemistry X, Journal Year: 2024, Volume and Issue: 21, P. 101164 - 101164

Published: Jan. 30, 2024

Thus far, little is known about whether jackfruit flake, a byproduct of jackfruit, can be used as fermentation substrate to obtain value-added products through microbial fermentation. Here, flake puree was fermented by three different ways: spontaneous (JF), inoculated with LAB (JFL), co-fermentation and yeast (JFC). In contrast JF, the total polyphenol flavonoid content syndrome-associated enzyme inhibition are significantly increased in JFC at end Electronic tongue analysis revealed that lower astringency higher bitterness. 41 volatile compounds were identified during HS-SPME-GC-MS, richer honey, rose, fruity flavors. A 290 screened for discriminative pre- post-fermentation differential metabolites non-target metabolomics analysis. These results provide potential reference conversion waste into functional using

Language: Английский

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Biotransformation and Gut Microbiota-Mediated Bioactivity of Flavonols

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(22), P. 8317 - 8331

Published: May 30, 2023

Consumption of fruits and vegetables has been associated with a reduced risk multiple diseases, such as metabolic disorders. Flavonols are the most ubiquitous flavonoids in vegetables. However, dietary flavonols exhibit general low oral bioavailability for their extensive biotransformation mediated by phase II enzymes enterocytes liver well microbiota gut lumen. In this context, have brought attention to paradox between health-promoting effects. often transformed prior absorption, which could change biological activity. Compared parent compounds, corresponding metabolites vivo might similar or higher intrinsic bioactivities, perhaps decreased efficacious effectiveness. Indeed, growing body evidence from function studies supports positive significant contribution processes, particularly conversion microbiota, benefits flavonols. As such, further understanding fate activities possible impact microbiota-mediated on bioactivity is great significance guide rational diet flavonol-rich and/or flavonol-containing functional foods.

Language: Английский

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Fecal microbiota transplantation improves hepatic fibro-inflammation via regulating oxidative stress in experimental NASH

Digestive and Liver Disease, Journal Year: 2023, Volume and Issue: 55(11), P. 1521 - 1532

Published: June 26, 2023

Nonalcoholic steatohepatitis (NASH) is associated with imbalance of gut microbiome, indicating participation environment in hepatic health status. Therefore, modulating via fecal microbiota transplantation (FMT) a promising therapeutic procedure for NASH patients. However, the effect and mechanism FMT remains largely unknown. Here, we investigated gut-liver axis to understand FMT-mediated improvement NASH. Feces from specific pathogen free mice were infused allogeneically into gastrointestinal tract fed high fat, cholesterol fructose (HFHCF), resulting suppressing pathogenic events, featured by decreasing inflammatory fibrotic mediators. The elevated NF-E2-related factor 2 (NRF2), key transcription that regulates antioxidant enzymes, livers. HFHCF-induced increased intestinal permeability abundant Facklamia Aerococcus, an imbalanced was significantly improved FMT, characterized restoration barrier function enrichment Clostridium. Notably, created inferred produce metabolites aromatic biogenic amine degradation pathway, specifically 4-hydroxyphenylacetic acid (4-HPA), which known ameliorate liver injury. We suggest gut-derived molecules, related such as 4-HPA are potential agents preventing treating

Language: Английский

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Identification of Bacterial Metabolites Modulating Breast Cancer Cell Proliferation and Epithelial-Mesenchymal Transition

Molecules, Journal Year: 2023, Volume and Issue: 28(15), P. 5898 - 5898

Published: Aug. 5, 2023

Breast cancer patients are characterized by the oncobiotic transformation of multiple microbiome communities, including gut microbiome. Oncobiotic impairs production antineoplastic bacterial metabolites. The goal this study was to identify metabolites with properties. We constructed a 30-member metabolite library and screened compounds for effects on cell proliferation epithelial-mesenchymal transition. were applied 4T1 murine breast cells in concentrations corresponding reference serum concentrations. However, yric acid, glycolic d-mannitol, 2,3-butanediol, trans-ferulic acid exerted cytostatic effects, 3-hydroxyphenylacetic 4-hydroxybenzoic vanillic hyperproliferative effects. Furthermore, hydrocinnamic inhibited epithelial-to-mesenchymal (EMT) identified redox sets among (d-mannitol—d-mannose, 1-butanol—butyric ethylene glycol—glycolic acid—oxalic acid), wherein only one partner within set (d-mannitol, butyric acid) possessed bioactivity our system, suggesting that changes local potential may affect secretome. Of nine bioactive metabolites, 2,3-butanediol compound both anti-EMT

Language: Английский

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