The Occurrence of Microplastics and the Formation of Biofilms by Pathogenic and Opportunistic Bacteria as Threats in Aquaculture

International Journal of Environmental Research and Public Health, Journal Year: 2022, Volume and Issue: 19(13), P. 8137 - 8137

Published: July 2, 2022

Aquaculture is the most rapidly growing branch of animal production. The efficiency and quality produced food depends on sustainable management, water quality, feed prices incidence diseases. Micro- (MP < 5 mm) nanoplastic (NP 1000 nm) particles are among current factors causing serious pollution. This substance comes solely from products manufactured by humans. MP migrate terrestrial to aquatic environment adversely affect, especially, health animals humans being a favorable habitat vector for microbial pathogens opportunists. More than 30 taxa humans, aquacutural plants, along with opportunistic bacteria, have been detected in plastic-covering biofilm date. mobility durability substance, combined relatively closed conditions aquacultural habitats pathogens’ affinity material, make plastic microbiological medium threatening industry aquaculture. For this reason, addition fact accumulation living organisms, urgent measures should be taken reduce its influx into environment. phenomenon implications related concept one health, wherein environment, affect each other’s fitness.

Language: Английский

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Microbial degradation and transformation of PPCPs in aquatic environment: A review

Heliyon, Journal Year: 2023, Volume and Issue: 9(8), P. e18426 - e18426

Published: July 19, 2023

The Pharmaceuticals and Personal Care Products (PPCPs) presence at harmful levels has been identified in aquatic ecosystems all over the world. Currently, PPCPs are more common regions have discovered to be extremely creatures. Waste-water treatment facilities primary cause of pollution systems due their limited as well following release PPCPs. degree elimination is primarily determined by method applied for remediation. It must addressed an eco-friendly manner order significantly improve environmental quality or, very least, prevent spread effects toxic pollutants. However, when compared other methods, environmentally friendly strategies (biological methods) less expensive require energy. Most biological methods under aerobic conditions shown degrade effectively. Furthermore, scientific literature indicates that with exception a few hydrophobic substances, degradation microbes process majority compounds. Hence, this review discusses about optimistic role microbe concerned or transformation into non/less form polluted environment. Accordingly, number microbial strains implicated biodegradation/transformation through termed bioremediation limitations.

Language: Английский

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A novel estrogen-targeted PEGylated liposome co-delivery oxaliplatin and paclitaxel for the treatment of ovarian cancer

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 160, P. 114304 - 114304

Published: Jan. 30, 2023

Ovarian cancer is the second cause of death among gynecological malignancies. In this study, we designed a novel estrogen-targeted PEGylated liposome loaded with oxaliplatin and paclitaxel (ES-SSL-OXA/PTX) which could target estrogen receptor (ER) highly expressed on surface SKOV-3 cells to enhance therapeutic efficacy reduce side effects for tumor therapy. ES-SSL-OXA/PTX was prepared by thin film hydration method exhibited uniform spherical morphology. Encapsulation efficiency (EE) were determined HPLC results 44.10% OXA 65.85% PTX. The mean particle size polydispersity index (PDI) 168.46 nm 0.145, respectively. vivo in vitro targeting study confirmed that has optimum specific ability. Meanwhile, antitumor superior antiproliferative effect stronger anti-tumor inhibition rate 85.24%. pharmacokinetics showed prolonged half-life time slowed clearance rate. preliminary safety acute toxicity long-term demonstrated reduced profile. Based above results, be promising formulation treatment ovarian future clinic.

Language: Английский

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Exploiting gender-based biomarkers and drug targets: advancing personalized therapeutic strategies in hepatocellular carcinoma

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: June 20, 2024

This review systematically examines gender differences in hepatocellular carcinoma (HCC), identifying the influence of sex hormones, genetic variance, and environmental factors on disease’s epidemiology treatment outcomes. Recognizing liver as a sexually dimorphic organ, we highlight how gender-specific risk factors, such alcohol consumption obesity, contribute differently to hepatocarcinogenesis men women. We explore molecular mechanisms, including differential expression androgen estrogen receptors, which mediate diverse pathways tumor biology cell proliferation, apoptosis, DNA repair. Our analysis underscores critical need for research cancer, from studies clinical trials, improve diagnostic accuracy therapeutic effectiveness. By incorporating perspective into all facets cancer research, advocate more precise personalized approach that acknowledges significant factor both progression HCC its response treatment. aims foster deeper understanding biological bases promote development tailored interventions enhance outcomes patients.

Language: Английский

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Sex reversal induced by 17β-estradiol may be achieved by regulating the neuroendocrine system of the Pacific white shrimp Penaeus vannamei

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 29, 2025

Due to sexual dimorphism in growth of penaeid shrimp, all-female cultivation is desirable for the aquaculture industry. 17β-estradiol (E2) has potential induce male-to-female sex reversal decapod species. However, mechanisms behind it remain poorly understood. This study aimed investigate effects E2 immersion on neuroendocrine system (the eyestalk ganglia, brain, thoracic and ventral nerve cord) Pacific white shrimp Penaeus vannamei through comparative transcriptomic analyses control males (CM), E2-treated without (EM), neo-females (NF). Immersion at 2 mg/l from post-larvae (PL) 5 85 results a skewed female-to-male ratio 2.56: 1. The survival rate remains unaffected by exposure, while notable inhibition exclusively observed EM compared CM group. transcriptome analysis result indicates that retardation may be attributed neuronal damage caused excessive neurotransmission, which turn disrupts PI3K-Akt signaling cell cycle pathways. In contrast, negatively regulated pathways neuroactive ligand-receptor interaction dopaminergic synapse NF group suggest impact neuroplasticity modulation neuroregulator binding signaling, affects establishment morphological differences gender identity. Moreover, activation steroidogenesis insulin associated with success E2-induced reversal. With these findings, proposed mechanism decapods response exposure presented. present represents first step understanding molecular level. disruptions Furthermore, downregulation interaction, synapse, pathways, as well steroidogenesis. These findings provide new insights into mode action E2, underscore large-scale production stocks P. vannamei.

Language: Английский

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A Fluorescence Biosensor with a Dual-function DNA Probe Targeting the Activated Estrogen Receptor for Estrogenic Activity Evaluation

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121163 - 121163

Published: Feb. 1, 2025

Language: Английский

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Epigenetics and Probiotics Application toward the Modulation of Fish Reproductive Performance

Fishes, Journal Year: 2022, Volume and Issue: 7(4), P. 189 - 189

Published: July 28, 2022

Fish represent an excellent source of animal protein as well a biomedical research model result their evolutionary relatedness and similarity with the human genome. Commercial ornamental fish culture has achieved popularity, but reproductive dysfunctions act limiting factor for quality fry production, interfering sustainability aquaculture industry. reproduction is crucial any species’ existence, performance can potentially be improved through applications epigenetics probiotics. Epigenetics highly sensitive molecular approach that includes chromatin structure function alteration, DNA methylation, modification non-coding RNA molecules transfer desired information from parents to offspring. methyltransferase improves cyp11a1, esr2b, figla gene expression feminizes zebrafish (Danio rerio). Moreover, also contributes genome stability, environmental plasticity, embryonic development. However, methylation specific genes negatively affect sperm quality, resulting in poor fertilization. Probiotic administration able induce responsiveness incompetent follicles maturation-inducing hormones change oocyte chemical composition during vitellogenic The positive role probiotics on testicular cells validated by upregulating transcription levels leptin, bdnf, dmrt1 facilitating spermatogenesis. This review not only discusses effects mechanism improving reproduction, presents overview causal factors current techniques used eradicate dysfunction. key related along gaps future prospects are considered. provides necessary students, scientists, researchers, breeders resolve reproduction-related problems ensure profitable sustainable aquaculture.

Language: Английский

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Biodegradation of 17β‐estradiol by drug‐resistant Stenotrophomonas maltophilia MN08 and Pseudomonas aeruginosa KL10 isolated from the sediment sample

Environmental Quality Management, Journal Year: 2024, Volume and Issue: 34(1)

Published: Jan. 27, 2024

Abstract 17 β‐Estradiol is produced by animals, and humans excreted through excreta, has endocrine‐disrupting activity. Bacterial degradation one of the suitable methods used for removal β‐estradiol (E2) from natural environment. In this study, E2‐degrading bacteria were screened enriched sediment sample wastewater Two bacterial strains ( Stenotrophomonas maltophilia MN08 Pseudomonas aeruginosa KL10) determined >70% E2‐degradation was achieved. The strain could rapidly remove approximately 86.5% ± 1.3% E2 within 7 days treatment. addition, KL10 removed 79.5% 1% same period. Optimized culture temperature pH medium improve E2‐degradation. results demonstrated that exhibited maximum activity at 35°C, 8.0. optimal to degrade ranged 7.0 9.0. level mixture systems can exhibit synergistic individual 87.3% 1.2% 81.4% 1.5%, respectively. potential mixed system 91.3% 2.9% under optimized conditions in which 100 mg/L served as sole source carbon energy. present finding demonstrates co‐culture S. P. may provide a promising bio‐treatment strategy steroids wastewater.

Language: Английский

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17α-Ethynylestradiol and Levonorgestrel Exposure of Rainbow Trout RTL-W1 Cells at 18 °C and 21 °C Mainly Reveals Thermal Tolerance, Absence of Estrogenic Effects, and Progestin-Induced Upregulation of Detoxification Genes

Genes, Journal Year: 2024, Volume and Issue: 15(9), P. 1189 - 1189

Published: Sept. 10, 2024

Fish are exposed to increased water temperatures and aquatic pollutants, including endocrine-disrupting compounds (EDCs). Although each stressor can disturb fish liver metabolism independently, combined effects may exist. To unveil the molecular mechanisms behind of EDCs temperature, cell lines potential models needing better characterisation. Accordingly, we rainbow trout RTL-W1 cells (72 h), at 18 °C 21 °C, ethynylestradiol (EE2), levonorgestrel (LNG), a mixture both hormones (MIX) 10 µM. The gene expression selection targets related detoxification (CYP1A, CYP3A27, GST, UGT, CAT, MRP2), estrogen exposure (ERα, VtgA), lipid (FAS, FABP1, FATP1), temperature stress (HSP70b) was analysed by RT-qPCR. GST higher after LNG than °C. further enhanced while MIX expressions CYP3A27 MRP2. In contrast, FAS only in MIX, compared control. ERα, VtgA, CYP1A, HSP70b, FATP1 were not influenced or tested EDCs. model unresponsive EE2 alone, sensitive (in pathway genes), mainly insensitive range but had specific interactions.

Language: Английский

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Association of ESR1 gene polymorphisms with the susceptibility to Hepatitis B virus infection and the clinical outcomes

Journal of Medical Virology, Journal Year: 2023, Volume and Issue: 95(2)

Published: Jan. 20, 2023

Estrogen receptor alpha (ESR1) has been implicated in the pathological process of Hepatitis B virus (HBV) infection and is probably an important determinant for gender differences. In this study, a total 975 subjects including 368 healthy controls, 323 hepatocellular carcinoma (HCC) patients with HBsAg positive, 284 HBV-infected without HCC were included. Three single nucleotide polymorphisms ESR1 (rs2234693, rs2077647, rs2228480) detected to investigate correlation between susceptibility HBV persistence clinical outcomes. The association prognosis was investigated our cohort enrolling 376 HBV-HCC patients. frequency rs2234693 C allele lower chronic (CHB) liver cirrhosis (LC) than that males (adjusted odds ratio [AOR] = 0.63, 95% confidence interval [CI] 0.41-0.96). rs2228480 A associated increased risk LC (AOR 2.20, CI 1.06-4.56) genotype C, significantly decreased recurrence (p 0.010) mRNA level tumor tissues 0.032). Haplotype C-G-G (OR 1.37, 1.08-1.73), while it opposite C-A-G T-G-G 0.41, 0.27-0.62; OR 0.53, 0.32-0.85, respectively). These results imply combinations these may be valuable prediction persistence.

Language: Английский

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