Microorganisms,
Journal Year:
2022,
Volume and Issue:
10(9), P. 1773 - 1773
Published: Sept. 2, 2022
The
increased
incidence
of
mixed
infections
requires
that
the
scientific
community
develop
novel
antimicrobial
molecules.
Essential
oils
and
their
bioactive
pure
compounds
have
been
found
to
exhibit
a
wide
range
remarkable
biological
activities
are
attracting
more
attention.
Therefore,
aim
this
study
was
evaluate
myrtenol
(MYR),
one
constituents
commonly
in
some
essential
oils,
for
its
potential
inhibit
biofilms
alone
combination
with
drugs
against
Candida
auris/Klebsiella
pneumoniae
single
biofilms.
activity
MYR
evaluated
by
determining
bactericidal/fungicidal
concentrations
(MIC),
biofilm
formation
at
sub-MICs
analyzed
96-well
microtiter
plate
crystal
violet,
XTT
reduction
assay,
CFU
counts.
synergistic
interaction
between
checkerboard
method.
exhibited
high
while
showing
efficient
antibiofilm
dual
To
understand
underlying
mechanism
which
promotes
single/mixed-species
inhibition,
we
observed
significant
downregulation
expression
mrkA,
FKS1,
ERG11,
ALS5
genes,
associated
bacterial
motility,
adhesion,
as
well
ROS
production,
can
play
an
important
role
inhibition
formation.
In
addition,
microdilution
assay
showed
strongly
both
caspofungin
(CAS)
meropenem
(MEM)
inhibiting
growth
pneumoniae-mixed
Furthermore,
tested
absence
toxicity
mammalian
cells
vitro
vivo
Galleria
mellonella
models.
Thus,
could
be
considered
agent
management
polymicrobial
Gels,
Journal Year:
2023,
Volume and Issue:
9(8), P. 626 - 626
Published: Aug. 3, 2023
Luliconazole
(LZ)
is
a
good
candidate
for
the
treatment
of
fungal
infection
topically
but
has
limitations,
i.e.,
poor
solubility
and
permeability
to
skin.
Due
these
multiple
administrations
long
time
are
required
treat
inflection.
The
aim
present
study
was
develop
invasomes
(IVS)
gel
LZ
improve
topical
antifungal
activity.
IVS
prepared
by
thin-film
hydration
method
optimized
Box-Bhekhen
design
software.
LZIVS
(LZIVSopt)
139.1
±
4.32
nm
vesicle
size,
88.21
0.82%
entrapment
efficiency,
0.301
0.012
PDI,
19.5
mV
(negative)
zeta
potential.
Scanning
microscopy
showed
spherical
shape
vesicle.
FTIR
spectra
there
no
interaction
between
drug
lipid.
Thermogram
that
encapsulated
into
matrix.
LZIVSopt
(LZIVSopt-G3)
exhibited
optimum
viscosity
(6493
27
cps)
significant
spreadability
(7.2
g·cm/s).
LZIVSopt-G3
2.47-fold
higher
permeation
than
pure
LZ-gel.
did
not
show
any
edema
or
swelling
in
skin,
revealing
developed
formulation
non-irritant.
inhibition
fungus
(C.
albicans)
infected
rats.
finding
concluded
carrier
an
attractive
approach
enhancement
delivery
infection.
Marine Drugs,
Journal Year:
2023,
Volume and Issue:
21(6), P. 350 - 350
Published: June 7, 2023
The
slow
discovery
of
new
antibiotics
combined
with
the
alarming
emergence
antibiotic-resistant
bacteria
underscores
need
for
alternative
treatments.
In
this
regard,
fish
skin
mucus
has
been
demonstrated
to
contain
a
diverse
array
bioactive
molecules
antimicrobial
properties,
including
peptides,
proteins,
and
other
metabolites.
This
review
aims
provide
an
overview
found
in
its
reported
vitro
capacity
against
bacteria,
fungi,
viruses.
Additionally,
different
methods
extraction,
which
can
be
grouped
as
aqueous,
organic,
acidic
extractions,
are
presented.
Finally,
omic
techniques
(genomics,
transcriptomics,
proteomics,
metabolomics,
multiomics)
described
key
tools
identification
isolation
compounds.
Overall,
study
provides
valuable
insight
into
potential
promising
source
agents.
Nanomaterials,
Journal Year:
2023,
Volume and Issue:
13(14), P. 2128 - 2128
Published: July 22, 2023
Full
and
partial
restorations
in
dentistry
must
replicate
the
characteristics
of
patient's
natural
teeth.
Materials
have
good
mechanical
properties
be
non-toxic
biocompatible.
Microbes,
which
can
form
biofilms,
are
constantly
contact
with
restorations.
In
this
study,
we
investigate
how
well
Candida
albicans
adheres
to
a
polymethyl
methacrylate
(PMMA)
resin
base
gold
(Au)
nanoparticles.
We
synthesized
Au
nanoparticles
characterized
them.
The
average
size
embedded
PMMA
was
11
nm.
color
difference
ΔE
between
PMMA/Au
composites
2.7
still
esthetically
acceptable
patients.
surfaces
smoother
more
hydrophilic
than
pure
surfaces,
isoelectric
point
both
types
4.3.
Above
point,
negatively
charged
surfaces.
added
decreased
tensile
strength,
while
hardness
did
not
change
significantly.
Adhesion
measurements
showed
that
modified
reduced
extent
microbial
adhesion
albicans.
Antimicrobial Agents and Chemotherapy,
Journal Year:
2023,
Volume and Issue:
67(6)
Published: May 17, 2023
Fungal
infections,
which
commonly
occur
in
immunocompromised
patients,
can
cause
high
morbidity
and
mortality.
Antifungal
agents
act
by
disrupting
the
cell
membrane,
inhibiting
nucleic
acid
synthesis
function,
or
β-1,3-glucan
synthase.
Because
incidences
of
life-threatening
fungal
infections
antifungal
drug
resistance
are
continuously
increasing,
there
is
an
urgent
need
for
development
new
with
novel
mechanisms
action.
Recent
studies
have
focused
on
mitochondrial
components
as
potential
therapeutic
targets,
owing
to
their
important
roles
viability
pathogenesis.
In
this
review,
we
discuss
drugs
targeting
highlight
unique
proteins
involved
electron
transport
chain,
useful
investigating
selective
targets.
Finally,
comprehensively
summarize
efficacy
safety
lead
compounds
clinical
preclinical
development.
Although
fungus-specific
mitochondrion
various
processes,
majority
target
dysfunction
mitochondria,
including
respiration
disturbance,
increased
intracellular
ATP,
reactive
oxygen
species
generation,
others.
Moreover,
only
a
few
under
trials,
necessitating
further
exploration
possible
targets
effective
agents.
The
chemical
structures
these
will
provide
valuable
hints
exploiting
antifungals.
Crystals,
Journal Year:
2022,
Volume and Issue:
12(6), P. 816 - 816
Published: June 8, 2022
Fungal
infections
caused
by
multidrug-resistant
strains
are
considered
one
of
the
leading
causes
morbidity
and
mortality
worldwide.
Moreover,
antifungal
medications
used
in
conventional
treatment
revealed
poor
therapeutic
effectiveness
possible
side
effects
such
as
hepatotoxicity,
nephrotoxicity,
myelotoxicity.
Therefore,
current
study
was
developed
to
determine
green
synthesized
silver
nanoparticles
(AgNPs)
their
synergistic
efficiency
with
drugs
against
candidal
strains.
The
AgNPs
were
greenly
using
aqueous
peel
extract
Punica
granatum.
In
addition,
characterized
ultraviolet-visible
spectrophotometry
(UV/Vis),
transmission
electron
microscopy
(TEM),
energy-dispersive
X-ray
spectroscopy
(EDX),
Fourier
transform
infrared
(FT-IR),
diffraction
analysis
(XRD),
zeta
potential
analysis.
this
regard,
UV-vis
indicated
SPR
at
396
nm,
while
particle
size
distribution
that
average
18.567
±
1.46
nm.
surface
charge
found
be
−15.6
mV,
indicating
stability
solutions.
biofabricated
activity
Candida
tropicalis,
C.
albicans,
glabrata
showing
inhibitory
zone
diameters
23.78
0.63,
21.38
0.58,
16.53
0.21
mm,
respectively
minimum
concentration
(MIC)
2.5
µg/mL
tropicalis
strain.
itraconazole
highest
strain,
glabrata,
recording
a
synergism
percentage
74.32%.
conclusion,
biogenic
combination
drug
exhibited
different
usage
bioformulation
highly
effective
agents.
International Journal of Oral Science,
Journal Year:
2023,
Volume and Issue:
15(1)
Published: Sept. 12, 2023
Candida
albicans
is
the
most
abundant
fungal
species
in
oral
cavity.
As
a
smart
opportunistic
pathogen,
it
increases
virulence
by
switching
its
forms
from
yeasts
to
hyphae
and
becomes
major
pathogenic
agent
for
candidiasis.
However,
overuse
of
current
clinical
antifungals
lack
new
types
drugs
highlight
challenges
antifungal
treatments
because
drug
resistance
side
effects.
Anti-virulence
strategy
proved
as
practical
way
develop
anti-infective
drugs.
Here,
seven
artemisinins,
including
artemisinin,
dihydroartemisinin,
artemisinic
acid,
dihydroartemisinic
artesunate,
artemether
arteether,
were
employed
target
at
hyphal
development,
important
factor
C.
albicans.
Artemisinins
failed
affect
growth,
but
significantly
inhibited
development
albicans,
azole
resistant
isolates,
reduced
their
damage
epithelial
cells,
while
arteether
showed
strongest
activities.
The
transcriptome
suggested
that
could
energy
metabolism
Seven
artemisinins
then
inhibit
productions
ATP
cAMP,
inhibition
on
RAS1
overexpression
strain
indicating
regulated
Ras1-cAMP-Efg1
pathway
development.
Importantly,
burden
infections
with
no
systemic
toxicity
murine
oropharyngeal
candidiasis
models
vivo
caused
both
fluconazole
sensitive
strains.
Our
results
first
time
indicated
can
be
potential
compounds
against
targeting
Journal of Obstetrics and Gynaecology,
Journal Year:
2023,
Volume and Issue:
43(1)
Published: April 8, 2023
At
concentrations
achieved
following
systemic
administration,
the
primary
effect
of
imidazoles
and
triazoles
on
fungi
is
inhibition
14-α-sterol
demethylase,
a
microsomal
cytochrome
P450
(CYP)
enzyme.
Imidazoles
impair
biosynthesis
ergosterol
for
cytoplasmic
membrane
lead
to
accumulation
14-α-methyl
sterols.
The
synthetic
imidazole
miconazole
additionally
able
increase
intracellular
reactive
oxygen
species,
at
least
in
part
through
fungal
catalase
peroxidase.
This
unique
feature
probably
basis
its
fungicidal
activity
C.
albicans,
addition
fungistatic
mode
action.
Studies
show
that
superior
nystatin
treatment
demonstrate
impact
as
one
best
options
managing
vulvovaginal
candidiasis.
Regarding
recurrent
candidiasis,
several
new
drugs
are
currently
developed
ensure
effective
also
this
group
patients.
