Genes,
Journal Year:
2024,
Volume and Issue:
15(12), P. 1640 - 1640
Published: Dec. 21, 2024
Background:
Cannabidiol,
which
is
one
of
the
main
cannabinoids
present
in
Cannabis
sativa
plants,
has
been
shown
to
have
therapeutic
properties,
including
anti-inflammatory
and
antioxidant
effects
that
may
be
useful
for
treatment
various
kidney
conditions.
Objectives:
This
article
investigates
effect
long-term
cannabidiol
(CBD)
on
changes
renal
transcriptome
a
mouse
model.
The
hypothesis
was
systematic
CBD
would
affect
gene
expression
associated
with
those
processes
kidney.
Methods:
study
conducted
male
C57BL/6J
mice.
Mice
experimental
groups
received
daily
intraperitoneal
injections
at
doses
10
mg/kg
or
20
body
weight
(b.w.)
28
days.
After
experiment,
tissues
were
collected,
RNA
isolated,
RNA-Seq
sequencing
performed.
Results:
results
show
CBD’s
expression,
regulation
genes
related
circadian
rhythm
(e.g.,
Ciart,
Nr1d1,
Nr1d2,
Per2,
Per3),
glucocorticoid
receptor
function
Cyp1b1,
Ddit4,
Foxo3,
Gjb2,
Pck1),
lipid
metabolism
Cyp2d22,
Cyp2d9,
Decr2
Hacl1,
Sphk1),
inflammatory
response
Cxcr4
Ccl28).
Conclusions:
obtained
suggest
beneficial
purposes
treating
disease,
its
should
further
analyzed
clinical
trials.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(14), P. 7530 - 7530
Published: July 9, 2024
Aging
is
a
multifaceted
process
influenced
by
hereditary
factors,
lifestyle,
and
environmental
elements.
As
time
progresses,
the
human
body
experiences
degenerative
changes
in
major
functions.
The
external
internal
signs
of
aging
manifest
various
ways,
including
skin
dryness,
wrinkles,
musculoskeletal
disorders,
cardiovascular
diseases,
diabetes,
neurodegenerative
cancer.
Additionally,
cancer,
like
aging,
complex
disease
that
arises
from
accumulation
genetic
epigenetic
alterations.
Circadian
clock
dysregulation
has
recently
been
identified
as
an
important
risk
factor
for
cancer
development.
Natural
compounds
herbal
medicines
have
gained
significant
attention
their
potential
preventing
age-related
diseases
inhibiting
progression.
These
demonstrate
antioxidant,
anti-inflammatory,
anti-proliferative,
pro-apoptotic,
anti-metastatic,
anti-angiogenic
effects
well
circadian
regulation.
This
review
explores
cancers,
specific
natural
targeting
key
features
these
conditions.
Biology,
Journal Year:
2023,
Volume and Issue:
12(3), P. 383 - 383
Published: Feb. 28, 2023
Most
lifeforms
on
earth
use
endogenous,
so-called
circadian
clocks
to
adapt
24-h
cycles
in
environmental
demands
driven
by
the
planet’s
rotation
around
its
axis.
Interactions
with
environment
change
over
course
of
a
lifetime,
and
so
does
regulation
clock
system.
In
this
review,
we
summarize
how
develop
humans
experimental
rodents
during
embryonic
development,
they
mature
after
birth
what
changes
occur
puberty,
adolescence
increasing
age.
Special
emphasis
is
laid
reproductive
systems
as
major
organizers
life
segments
span.
We
discuss
differences
sexes
outline
potential
areas
for
future
research.
Finally,
options
medical
applications
lifespan
chronobiology
are
discussed.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(3), P. 2407 - 2407
Published: Jan. 26, 2023
Clock
(circadian)
genes
are
heterogeneously
expressed
in
hair
follicles
(HFs).
The
can
be
modulated
by
both
the
central
circadian
system
and
some
extrinsic
factors,
such
as
light
thyroid
hormones.
These
participate
regulation
of
several
physiological
processes
HFs,
including
growth
pigmentation.
On
other
hand,
because
peripheral
synchronized
with
clock,
HFs
could
provide
a
noninvasive
practical
method
for
monitoring
evaluating
multiple
circadian-rhythm-related
conditions
disorders
among
humans,
day
night
shifts,
sleep-wake
disorders,
physical
activities,
energy
metabolism,
aging.
However,
due
to
complexity
biology,
understanding
how
intrinsic
oscillation
operates
using
tissues
only
may
insufficient.
Combining
HF
sampling
multidimensional
assays
detection
body
temperature,
blood
samples,
or
certain
validated
questionnaires
helpful
improving
applications.
Thus,
serve
critical
model
clock
help
an
potential
mechanisms
conditions;
furthermore,
chronotherapy
support
personalized
treatment
scheduling
based
on
gene
expression
profile
HFs.
Aging,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 6, 2024
Observational
studies
in
preclinical
models
demonstrate
age-related
declines
circadian
functions.
We
hypothesized
that
age
would
be
associated
with
function
of
cell-autonomous
clocks
human
tissue.
Accordingly,
we
cultured
adipose
progenitor
cells
(APCs)
from
previously
collected
white-adipose
tissue
biopsies
abdominal
subcutaneous
depots
young
(Age:
23.4
±
2.1
yrs)
vs.
older
female
participants
70.6
5.9
yrs).
Using
an
vitro
model,
compared
rhythmic
gene
expression
profiles
core
clock
components,
as
indicator
oscillatory
function.
observed
consistent
rhythmicity
components
and
older-APCs.
Expression
analysis
showed
increased
levels
some
older-APCs
(CLOCK,
CRY1,
NR1D1)
young.
also
investigated
resveratrol
(RSV),
a
well-known
longevity-enhancing
effector,
for
its
effects
on
profiles.
found
RSV
resulted
gained
(CLOCK
CRY),
loss
others
(PER2,
CRY2),
altered
parameters
(NR1D1
NR1D2),
The
observation
detectable
retained
suggests
the
APCs
is
preserved
at
this
stage
aging
process.
impacts
APCs,
implicating
potential
therapeutic
agent
longevity
by
targeting
clock.
International Journal of Medical Sciences,
Journal Year:
2025,
Volume and Issue:
22(5), P. 1138 - 1149
Published: Feb. 10, 2025
Background:
Rat
models
are
widely
used
to
study
cataracts
due
their
cost-effectiveness
and
prominent
physiological
genetic
similarities
humans
The
objective
of
this
was
identify
genes
involved
in
cataractogenesis
galactose
exposure
rats.
Methods:
We
analyzed
four
datasets
from
the
Gene
Expression
Omnibus,
including
both
ex
vivo
different
rat
strains.
Feature
selection
tools
were
potentially
relevant
cataract-related
gene
expression.
A
decision
tree
algorithm
implemented,
its
predictions
interpreted
using
SHAP
LIME.
To
validate
expression
levels,
PCR
conducted
on
six
lenses
cultured
M199
medium
induce
cataract
alone.
Results:
Using
feature
tools,
key
genes-PLAGL2,
CMTM7,
PCYT1B,
NR1D2-were
identified.
Only
PCYT1B
significantly
differentially
expressed
between
control
groups
across
datasets.
model
showed
strong
predictive
performance,
particularly
LIME
analyses
revealed
that
CMTM7
had
largest
impact
predictions.
results
did
not
show
significant
differences
groups.
Conclusion:
trained
an
dataset
could
predict
despite
no
found
Given
a
small
number
samples,
larger
studies
needed
our
findings.
Military Medical Research,
Journal Year:
2023,
Volume and Issue:
10(1)
Published: Dec. 11, 2023
Abstract
Nuclear
receptor
subfamily
1,
group
D,
member
1
(NR1D1,
also
known
as
REV-ERBα)
belongs
to
the
nuclear
(NR)
family,
and
is
a
heme-binding
component
of
circadian
clock
that
consolidates
oscillators.
In
addition
repressing
transcription
multiple
genes
associated
with
rhythms,
NR1D1
has
wide
range
downstream
target
are
intimately
involved
in
many
physiopathological
processes,
including
autophagy,
immunity,
inflammation,
metabolism
aging
organs.
This
review
focuses
on
pivotal
role
key
factor
gene
regulatory
network,
particular
emphasis
milestones
latest
discoveries
ligands.
considered
promising
drug
for
treating
diverse
diseases
may
contribute
research
innovative
biomarkers
therapeutic
targets
organ
injury-related
diseases.
Further
ligands
prospective
human
trials
pave
way
their
clinical
application
disorders.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(23), P. 12899 - 12899
Published: Nov. 30, 2024
Maternal
unbalanced
diets
cause
adverse
metabolic
programming
and
affect
the
offspring's
liver
microRNA
(miRNA)
profile.
The
is
a
site
of
β-carotene
(BC)
metabolism
target
BC
action.
We
studied
interaction
maternal
Western
diet
(WD)
early-life
supplementation
on
epigenetic
remodeling
microRNAs.
Mouse
offspring
WD-fed
mothers
were
given
daily
placebo
(controls)
or
during
suckling.
Biometric
parameters
miRNAome
by
microarray
hybridization
analyzed
in
newly
weaned
animals.
sex-dependently
impacted
triacylglycerol
content.
was
also
differently
affected
male
female
offspring,
with
no
overlap
differentially
expressed
(DE)
miRNAs
between
sexes
more
impact
females.
Bioinformatic
analysis
DE
miRNA
predicted
genes
revealed
enrichment
biological
processes/pathways
related
to
processes,
regulation
developmental
growth
circadian
rhythm,
homeostasis
metabolism,
insulin
resistance,
neurodegeneration,
among
others,
differences
sexes.
Fifty-five
percent
overlapping
both
identified
targeted
changed
opposite
directions
males
results
identify
sex-dependent
responses
expression
profile
suckling
may
sustain
further
investigations
regarding
long-term
early
postnatal
life
top
an
diet.
