Bacillus halotolerans SW207 alleviates enterotoxigenic Escherichia coli -induced inflammatory responses in weaned piglets by modulating the intestinal epithelial barrier, the TLR4/MyD88/NF-κB pathway, and intestinal microbiota

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(4)

Published: March 7, 2024

Enterotoxigenic Escherichia coli (ETEC) is one of the major pathogens contributing to piglet diarrhea, with significant implications for both health and economic aspects livestock industry. SW207 an isolate Bacillus halotolerans isolated from cold- disease-resistant Leixiang pigs in Northeastern China. We have discovered that can survive pig's gastrointestinal fluid under conditions high bile salt concentration, displaying potent antagonistic activity against ETEC. In this study, we established a weaned diarrhea model infected ETEC investigate role preventing improving intestinal health. Results indicate upregulates expression tight junction proteins, including claudin-1, occludin, zonula occludens-1, at transcriptional translational levels. Furthermore, reduces serum endotoxin, D-lactic acid, various oxidative stress markers while enhancing mechanical barrier function. terms immune barrier, suppressed activation TLR4/MyD88/NF-κB pathway, reducing inflammatory factors upregulating small intestine mucosal sIgA. Concerning biological significantly content E. intestines promotes abundance beneficial bacteria, thereby mitigating microbiota imbalance caused by summary, has potential prevent ETEC, alleviate inflammation epithelial damage, facilitate changes microbiota. This contributes elucidating mechanisms host-microbe interactions pathogen infections.IMPORTANCEEnterotoxigenic consistently been causing mortality piglets pig farming. The industry traditionally relied on antibiotic administration control ETEC-induced diarrhea. However, overuse antibiotics led emergence drug-resistant zoonotic bacterial pathogens, posing threat public Therefore, there urgent need identify alternatives reduce usage. assessed protective effect novel probiotic analyzed its vivo vitro. study results provide theoretical support reference implementing interventions gut early improve

Language: Английский

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Potential Benefits of Coffee Consumption on Improving Biomarkers of Oxidative Stress and Inflammation in Healthy Individuals and Those at Increased Risk of Cardiovascular Disease

Molecules, Journal Year: 2023, Volume and Issue: 28(18), P. 6440 - 6440

Published: Sept. 5, 2023

Cardiovascular diseases (CVDs) are considered the predominant cause of death globally. An abnormal increase in biomarkers oxidative stress and inflammation consistently linked with development even progression metabolic diseases, including enhanced CVD risk. Coffee is one most consumed beverages world, while reviewed evidence regarding its capacity to modulate remains limited. The current study made use prominent electronic databases, PubMed, Google Scholar, Scopus retrieve information from randomized controlled trials reporting on any association between coffee consumption modulation healthy individuals or those at increased risk developing CVD. In fact, summarized indicates that consumption, mainly due abundant antioxidant properties, can reduce inflammation, which be essential alleviating individuals. However, more suggests regular/prolonged long term (>4 weeks) appeared beneficial comparison short-term intake (<4 weeks). These positive effects also observed already presenting risk, although such very analysis data highlights importance understanding how strengthening intracellular antioxidants alleviate pathological features within general population. Also covered review metabolism bioavailability profile coffee, especially caffeine as major bioactive compounds.

Language: Английский

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Chemo-proteomics reveals dihydrocaffeic acid exhibits anti-inflammation effects via Transaldolase 1 mediated PERK-NF-κB pathway

Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 5, 2025

Acute pneumonia is a kind of widespread inflammatory pathological process. Dihydrocaffeic acid (DA), metabolite chlorogenic acid, possesses potent pharmacologic activity for the therapy wide range disorders and various biological properties, such as anti-inflammation. Nevertheless, specific protein targets potential molecular mechanisms DA in acute are still poorly understood. To investigate anti-inflammation effects its target mechanisms. Here, we conducted lipopolysaccharides (LPS)-induced model mice. Besides, activity-based profiling (ABPP) was performed to explore DA. Furthermore, cellular thermal shift assay (CETSA) pulldown-western blot assays were used validate conclusion. In this study, indicated that alleviated mice displayed excellent anti-inflammatory efficacy vivo vitro. discovered binds directly transaldolase 1(TALDO1) influenced enzymatic activity, identified cysteine sites Cys250. Also demonstrated reveals effect through TALDO1 mediated PERK-IκBα-NF-κB pathway RAW 264.7 cells. This study provide support advancement use therapeutic agent treatment inflammation-associated diseases.

Language: Английский

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3-(4-Hydroxy-3-methoxyphenyl) propionic acid contributes to improved hepatic lipid metabolism via GPR41

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Dec. 1, 2023

Abstract 3-(4-hydroxy-3-methoxyphenyl) propionic acid (HMPA) is a metabolite produced by the gut microbiota through conversion of 4-hydroxy-3-methoxycinnamic (HMCA), which widely distributed hydroxycinnamic acid-derived found abundantly in plants. Several beneficial effects HMPA have been suggested, such as antidiabetic properties, anticancer activities, and cognitive function improvement, animal models human studies. However, intricate molecular mechanisms underlying bioaccessibility bioavailability profile following intake substantial modulation metabolic homeostasis require further elucidation. In this study, we effectively identified characterized HMPA-specific GPR41 receptor, with greater affinity than HMCA. The activation receptor plays crucial role anti-obesity improvement hepatic steatosis stimulating lipid catabolism pathway. For disorders, our results provide insights into development functional foods, including HMPA, preventive pharmaceuticals targeting GPR41.

Language: Английский

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The colonic polyphenol catabolite dihydroferulic acid (DHFA) regulates macrophages activated by oxidized LDL, 7-ketocholesterol, and LPS switching from pro- to anti-inflammatory mediators

Food & Function, Journal Year: 2024, Volume and Issue: 15(20), P. 10399 - 10413

Published: Jan. 1, 2024

Macrophage activation plays a central role in the development of atherosclerotic plaques.

Language: Английский

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Dihydrocaffeic Acid—Is It the Less Known but Equally Valuable Phenolic Acid?

Biomolecules, Journal Year: 2023, Volume and Issue: 13(5), P. 859 - 859

Published: May 18, 2023

Dihydrocaffeic acid (DHCA) is a phenolic bearing catechol ring and three-carbon side chain. Despite its being found in minor amounts numerous plants fungi of different origins, it has attracted the interest various research groups many fields science, from food to biomedical applications. The review article presented herein aims show wider audience health benefits therapeutic, industrial, nutritional potential dihydrocaffeic acid, by sheddinglight on occurrence, biosynthesis, bioavailability, metabolism. scientific literature describes at least 70 derivatives both those occurring naturally obtained via chemical enzymatic methods. Among most frequently used enzymes that were applied for modification parent DHCA structure, there are lipases allow obtaining esters phenolidips, tyrosinases formation ring, laccases functionalize this acid. In studies, vitro vivo, protective effect cells subjected oxidative stress inflammation acknowledged.

Language: Английский

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Antioxidant and Anti-inflammatory Effects of Marine Phlorotannins and Bromophenols Supportive of Their Anticancer Potential

Nutrition Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: June 18, 2024

Abstract Following the goal of optimizing nutrition, food industry has been continuously working on reformulation, nutritional patterns, functional foods development, and general promotion a healthy lifestyle. To this end, scientific community increasingly investigating natural compounds that could prevent or treat chronic diseases. Phlorotannins bromophenols are phenolic particularly present in marine organisms. There is extensive evidence shows their potential prevention noncommunicable diseases, including cancer, second cause mortality worldwide. Numerous studies have demonstrated anticarcinogenic activity polyphenolic algae both cell culture experimental animal models. Although recent reviews also available, update focuses most findings related to antioxidant/anti-inflammatory effect seaweed phenolics, as well regulatory capacity for new molecular targets. Additionally, review addresses discusses close link between inflammation oxidative stress, along with relationship tumor onset progression, supporting correlation. clinical still needed support evidence, phlorotannins constitute an emerging bioactive group high chemopreventive agents and/or adjuvants existing cancer therapies.

Language: Английский

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Main Colonic Metabolites from Coffee Chlorogenic Acid May Counteract Tumor Necrosis Factor-α-Induced Inflammation and Oxidative Stress in 3T3-L1 Cells

Molecules, Journal Year: 2023, Volume and Issue: 29(1), P. 88 - 88

Published: Dec. 22, 2023

Obesity is coupled with an altered redox state and low-level inflammation. Oxidative stress may increase pre-adipocyte proliferation, adipocyte differentiation mature size. Regarding inflammation, the dysregulation of cytokine production by adipose tissue takes place in obesity, which promoted oxidative stress. Polyphenols exert a positive effect on not only modulating state, but also due to their anti-inflammatory activity. Coffee, one most consumed beverages, very rich phenolic compounds. Bioavailability studies coffee phenols have shown that abundant group metabolites plasma urine are dihydrocaffeic (DHCA), dihydroferulic (DHFA), hydroxyhippuric (HHA) acids, three acids colonic origin. To better understand antioxidant properties DHCA, DHFA, HHA, inflammation/oxidation model was set up 3T3-L1 cell line using tumor necrosis factor-α (TNF-α). After exposure cells 0.5, 1, 5, 10 µM TNF-α at different times, viability, interleukin (IL)-6 secretion, reactive oxygen species (ROS) glutathione (GSH) were determined. Using prooxidant proinflammatory conditions established (10 µM, 24 h), it observed physiological concentrations (0.5, µM) HHA induced dose-dependent effects according ROS, GSH, enzyme (glutathione peroxidase) results. In addition, reductions IL-1β, IL-6, monocyte chemoattractant protein-1 (MCP-1) extents depending metabolite (DHFA, or DHCA) concentration used. conclusion, main from chlorogenic counteract TNF-α-induced inflammation line, thus, they present antiobesity potential.

Language: Английский

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Highlighting the Use of the Hepatoprotective Nutritional Supplements among Patients with Chronic Diseases

Healthcare, Journal Year: 2023, Volume and Issue: 11(19), P. 2685 - 2685

Published: Oct. 6, 2023

Cross-sectional studies, while not considered glamorous endeavors, are firmly anchored in data and statistics, providing essential insights about public health. The aim of the study is to see frequency hepatoprotective (HP) nutritional supplement consumption among patients with chronic diseases (other than liver disorders) analyzes habits related supplements these patients. A total 954 patients, seeking medical prescriptions for under various payment arrangements (compensated, gratuity, or full payment) were carefully selected over a 12-month period from four private pharmaceutical facilities. We examined HP relation number prescribed medications conditions. All invited complete questionnaire their provided option participate status assessment. One hundred ninety-five consented survey, 65 agreed undergo evaluation. Of 77.2% incorporate into regimen. most frequent (83.33%) was recorded group seven drugs, followed by three drugs (82.84%). Women have higher usage rate (80.58%; 444 551) compared men (62.60%; 293 383), (59.5%) used extracts Silybum marianum L. In 64.61% participants using supplements, (59.52%) consuming HP. Only 32.54% rely on recommendations healthcare professionals. who use 55.56% reported improvements health status. Furthermore, integrate daily routine tend achieve better overall

Language: Английский

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Stochastic Dynamics Mass Spectrometry of Caffeine Metabolites

SSRN Electronic Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The term structural analysis shall be used to refer innovative stochastic dynamics mass spectrometric equations [equation] and utilised for determining three-dimensional molecular structures of caffeine metabolites theoretically predict their spectra. study examines caffeine; uric acid, 1-methyl-, 1,2-, 1,7-, 3,7-dimethyl-uric acid; xanthine; 1-, 3-, 7-methyl-xanthine; para- hydroxy-xanthines; theacrine; theobromine; guanine; thymine; theophylline, respectively. It employs ultra-high-resolution electrospray ionisation spectrometry ion mobility spectrometry; high accuracy quantum chemical static methods based on ab initio density functional theory. Chemometrics assesses degree statistical correspondence between observable theoretical data these analyte measurands. soft-ionisation exhibits very wide range research applications analysis. encompasses fields chemistry, biology, medicine, more; instance, environmental polymer petroleum chemistry; clinical diagnostics; forensic toxicology, laboratory medicine; pharmacy; biochemistry; nuclear forensics; agricultural food science; geology; archaeology; etc. is also crucial developing formulas have a broad spectrum interdisciplinary both fundamental science industry. gives empirical support central claim providing exact electronic data. are essential understanding reliable quantitative treatment achieved excellent-to-exact chemometric method performances assessing links experimental parameters indicate that the grasp correctly variables provide analytical results. reader might see complementary application spectrometry, focusing knowledge monomers dimeric self-associates species, tautomers protomers. work determines structurally isotopologies. They sought advantage through isotopomeric fine isotope distribution. increases reliability low- high-molecular weight analytes; thus, causing superior omics-protocols. task primary purposes precision medicine. Data caffeine, para-xanthine, theobromine isotopomers protomers fluctuations in elemental composition via formulas, shown above yield chemometrics (|r|=0.99986–0.99957.) Analysis dimers shows |r|=0.99989. enabled review perspective changing concepts data-processing measurands; seeing as prospective tool analytes, protomers, tautomers, isotopomers.

Language: Английский

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