Geraniol modulates inflammatory and antioxidant pathways to mitigate intestinal ischemia–reperfusion injury in male rats

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Language: Английский

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High glucose dialysate-induced peritoneal fibrosis: Pathophysiology, underlying mechanisms and potential therapeutic strategies

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 165, P. 115246 - 115246

Published: July 29, 2023

Peritoneal dialysis is an efficient renal replacement therapy for patients with end-stage kidney disease. However, continuous exposure of the peritoneal membrane to dialysate frequently leads fibrosis, which alters function and results in withdrawal from patients. Among others, high glucose considered as a predisposing factor fibrosis on dialysis. Glucose-induced inflammation, metabolism disturbance, activation renin-angiotensin-aldosterone system, angiogenesis noninflammation-induced reactive oxygen species are implicated pathogenesis dialysate-induced fibrosis. Specifically, causes chronic inflammation recurrent peritonitis, could cause migration polarization inflammatory cells, well release cytokines High also interferes lipid glycolysis by activating sterol-regulatory element-binding protein-2/cleavage-activating protein pathway increasing hypoxia inducible factor-1α expression, leading Activation system Ras-mitogen activated kinase signaling another contributing Ultimately, transforming growth factor-β1/Smad involved mesothelial-mesenchymal transition or epithelial-mesenchymal transition, development Although possible intervention strategies targeting have occasionally been proposed, lack laboratory evidence renders clinical decision-making difficult. We therefore aim revisit upstream pathways factor-beta1/Smad propose potential therapeutic targets glucose-induced

Language: Английский

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Exploring the Impact of Cyanidin-3-Glucoside on Inflammatory Bowel Diseases: Investigating New Mechanisms for Emerging Interventions

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(11), P. 9399 - 9399

Published: May 28, 2023

Cyanidin-3-O-glucoside (C3G), the most widely distributed anthocyanin (ACN) in edible fruits, has been proposed for several bioactivities, including anti-inflammatory, neuro-protective, antimicrobial, anti-viral, anti-thrombotic and epigenetic actions. However, habitual intake of ACNs C3G may vary among populations, regions, seasons, individuals with different education financial status. The main point absorption occurs small large bowel. Therefore, it supposed that treating properties might affect inflammatory bowel diseases (IBD), such as ulcerative colitis (UC) Crohn's disease (CD). IBDs develop through complex pathways sometimes be resistant to conventional treatment strategies. presents antioxidative, cytoprotective, antimicrobial effects useful IBD management. In particular, studies have demonstrated inhibits NF-κB pathway activation. addition, activates Nrf2 pathway. On other hand, modulates expression antioxidant enzymes cytoprotective proteins, NAD(P)H, superoxide dismutase, heme-oxygenase (HO-1), thioredoxin, quinone reductase-oxide 1 (NQO1), catalase, glutathione S-transferase peroxidase. Interferon I II are downregulated by inhibiting interferon-mediating cascades. Moreover, reduces reactive species pro-inflammatory cytokines, C protein, interferon-γ, tumor necrosis factor-α, interleukin (IL)-5, IL-9, IL-10, IL-12p70, IL-17A UC CD patients. Finally, gut microbiota inducing an increase beneficial bacteria increasing microbial abundances, thus mitigating dysbiosis. Thus, activities potential therapeutic protective actions against IBD. Still, future, clinical trials should designed investigate bioavailability patients proper doses sources, aiming standardization exact outcome efficacy C3G.

Language: Английский

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Exploring the Pathophysiological Mechanisms of Acute Gastrointestinal Injury in Acute Exacerbations of Chronic Obstructive Pulmonary Disease: An Integrative Review

Journal of Contemporary Medical Practice, Journal Year: 2025, Volume and Issue: 7(1), P. 212 - 217

Published: Jan. 31, 2025

Acute gastrointestinal injury (AGI) complicating acute exacerbations of chronic obstructive pulmonary disease (AECOPD) represents a complex multisystem pathophysiological phenomenon involving systemic inflammation, hypoxemia, microcirculatory dysfunction, and gut microbiota dysbiosis. Inflammatory mediators, such as tumor necrosis factor-alpha (TNF-α) interleukin-6 (IL- 6), along with hypoxia-inducible factor-1 alpha (HIF-1α), serve key drivers dysfunction. Furthermore, disturbances dysregulated gut-lung axis interactions exacerbate barrier impairment. This review systematically analyzes the mechanisms AECOPD complicated by AGI, focusing on hypoxia, imbalance, aiming to provide theoretical foundation reference for clinical management.

Language: Английский

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Elucidating the Role of HIF-1α/YAP Signaling Pathway in Regulating Inflammation in Human Periodontal Stem Cells: An in vitro Study

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 1875 - 1886

Published: Feb. 1, 2025

Background and Objective: Periodontitis is a chronic inflammatory disease caused by dental plaque accumulation, leading to damage of periodontal tissues potential tooth loss. Understanding the mechanisms periodontitis, particularly role hypoxia in inflammation, critical for identifying novel therapeutic strategies. This study investigated effects prolyl hydroxylase (PHD) inhibitor DMOG on pro-inflammatory cytokine expression human ligament stem cells (hPDLSCs) examined involvement HIF-1α/YAP signaling path ay modulating inflammation. Materials Methods: hPDLSCs were cultured treated with lipopolysaccharide (LPS) induce followed treatment. Cell proliferation was assessed using CCK-8 assay, while ELISA RT-qPCR evaluated levels HIF-1α, IL-1β, TNF-α, YAP. YAP knocked down siRNA transfection examine its cytokines. Results: significantly increased HIF-1α reducing IL-1β TNF-α LPS-treated hPDLSCs. 0.1 mmol/L inhibited cell after 72 hours (P < 0.001). results showed that concentrations LPS + group higher than 0.01), reduced 0.01). confirmed these trends, showing mRNA 0.0001). knockdown via reversed effects, increasing 0.01) 0.05). Conclusion: demonstrated reduces stabilizing activating pathway. These findings provide mechanistic basis targeting axis control inflammation support PHD inhibitors as agents periodontitis. Keywords: YAP, DMOG,

Language: Английский

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Construction and validation of a prediction nomogram model for acute gastrointestinal failure in patients with severe traumatic brain injury

Medicine, Journal Year: 2025, Volume and Issue: 104(6), P. e41423 - e41423

Published: Feb. 7, 2025

This study aims to establish and validate the prediction model of acute gastrointestinal failure (AGF) in patients with severe traumatic brain injury. A total 665 inpatients from Shaoxing People’s Hospital January 2018 2024 were admitted randomly divided into training group (466 cases) validation (199 cases). Data collected by general situation questionnaire AGF assessment tool. According results multivariate logistic regression analysis, nomogram was established R software. Bootstrap method used for internal verification model, external verification. The area under receiver operating characteristic (ROC) curve, Hosmer–Lemeshow test calibration curves evaluate differentiation degree model. Multivariate Logistic analysis showed that pulmonary infection, hypoxemia, glasgow coma scale (GCS) score ≤ 8 on admission, hyponatremia metabolic acidosis independent risk factors injury ( P < .05). On this basis, a new constructed, as follows: logit = −4.998 + 0.858 × infection 0.923 hypoxemia 1.488 GCS 1.274 1.020 acidosis. ROC 0.787 (95% CI: 0.831–0.909), cutoff point 0.4589. sensitivity specificity 69.74% 76.15%, respectively. goodness fit had good fitting effect χ 2 4.828, .681). External 12.712, .122). Calibration fits well real data. constructed has degree, which can intuitively easily select high-risk patients, provide reference early screening nursing intervention.

Language: Английский

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Structure-Based Modeling of the Gut Bacteria–Host Interactome Through Statistical Analysis of Domain–Domain Associations Using Machine Learning

BioTech, Journal Year: 2025, Volume and Issue: 14(1), P. 13 - 13

Published: Feb. 25, 2025

The gut microbiome, a complex ecosystem of microorganisms, plays pivotal role in human health and disease. microbiome's influence extends beyond the digestive system to various organs, its imbalance is linked wide range diseases, including cancer neurodevelopmental, inflammatory, metabolic, cardiovascular, autoimmune, psychiatric diseases. Despite significance, interactions between bacteria proteins remain understudied, with less than 20,000 experimentally validated protein host any species. This study addresses this knowledge gap by predicting protein-protein interaction network bacterial proteins. Using statistical associations Pfam domains, comprehensive dataset over one million pan-bacterial-human interactions, as well inter- intra-species from organisms, were used for development machine learning-based prediction method uncover key regulatory molecules dynamic system. study's findings contribute understanding intricate microbiome-host relationship pave way future experimental validation therapeutic strategies targeting microbiome interplay.

Language: Английский

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KunMingShanHaiTang formula reprograms macrophage metabolism and promotes M2 polarization via the HIF-1α pathway to alleviate ulcerative colitis symptoms in a rat model

Journal of Bioenergetics and Biomembranes, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

The KunMingShanHaiTang Formula (KMSHTF), adjusted by Professor Zhong Chuanhua for the treatment of ulcerative colitis (UC), is work a renowned veteran practitioner Chinese medicine. However, its specific mechanism remains unknown. Consequently, it intriguing to investigate molecular which KMSHTF treats UC. To elucidate in UC rats. Initially, active ingredients and key target genes treating were analyzed using network pharmacology. Protein-Protein interaction gene enrichment analyses performed predict targets pathways. Subsequently, rats treated with KMSHTF, expression proteins intestinal tissue detected. Finally, compounds intreating further screened Molecular Docking, their pharmacological effects validated through cell experiments. A total 47 365 identified Traditional Medicine Systems Pharmacology Database Analysis Platform,along GeneCards database. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) Enrichment revealed that exerted therapeutic on regulating multiple In this study, HIF-1α pathway was selected as main UC, validation conducted vivo vitro experiments.Animal studies significantly ameliorated symptoms rats, including diarrhea,rectal bleeding pathological alterations wall. Furthermore, reduced pro-inflammatory cytokines IL-6 TNF-α, up-regulated IL-4 M2 macrophages down-regulated iNOS IL-1β M1 macrophages. Additionally, decreased levels HKII GLUT1 related pathway. three Baicalein, Palmatine Triptonide-were based strong binding affinity computational docking. Cellular experiments demonstrated each these downregulated protein HIF-1α, HKII, an wall model. Of Note, Baicalein exhibited most pronounced effect. overexpression reversed Baicalein-induced downregulation at level vitro. may modulate macrophage metabolism promote polarization pathway, thereby contributing efficacy (UC). Palmatine, Triptonide are core primarily contribute hypothesis.

Language: Английский

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Crosstalk between hypoxia-inducible factor-1α and short-chain fatty acids in inflammatory bowel disease: key clues toward unraveling the mystery

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 28, 2024

The human intestinal tract constitutes a complex ecosystem, made up of countless gut microbiota, metabolites, and immune cells, with hypoxia being fundamental environmental characteristic this ecology. Under normal physiological conditions, delicate balance exists among these “residents”, disruptions potentially leading to inflammatory bowel disease (IBD). core pathology IBD features disrupted epithelial barrier, alongside evident microecological disturbances. Central interconnected networks is hypoxia-inducible factor-1α (HIF-1α), which key regulator in cells for adapting hypoxic conditions maintaining homeostasis. Short-chain fatty acids (SCFAs), as pivotal serve vital mediators between the host significantly influence ecosystem. Recent years have seen surge research on roles therapeutic potential HIF-1α SCFAs independently, yet reviews HIF-1α-mediated regulation under are scarce. This article summarizes evidence interplay regulatory relationship IBD, elucidating disease’s pathogenesis offering promising strategies.

Language: Английский

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The role of oxygen tension in cell fate and regenerative medicine: implications of hypoxia/hyperoxia and free radicals

Cell and Tissue Banking, Journal Year: 2023, Volume and Issue: 25(1), P. 195 - 215

Published: June 26, 2023

Language: Английский

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