Antifungal Properties of Biogenic Selenium Nanoparticles Functionalized with Nystatin for the Inhibition of Candida albicans Biofilm Formation DOI Creative Commons
Shivraj Hariram Nile, Dipalee K. Thombre,

Amruta Shelar

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(4), P. 1836 - 1836

Published: Feb. 15, 2023

In the present study, biogenic selenium nanoparticles (SeNPs) have been prepared using Paenibacillus terreus and functionalized with nystatin (SeNP@PVP_Nystatin nanoconjugates) for inhibiting growth, morphogenesis, a biofilm in Candida albicans. Ultraviolet-visible spectroscopy analysis has shown characteristic absorption at 289, 303, 318 nm, X-ray diffraction peaks different 2θ values SeNPs. Electron microscopy that SeNPs are spherical shape size range of 220-240 nm. Fourier transform infrared confirmed functionalization on (formation SeNP@PVP_Nystatin nanoconjugates), zeta potential negative charge nanoconjugates. Biogenic inactive; however, nanoconjugates antifungal activities C. albicans (inhibited biofilm). The molecular mechanism action via real-time polymerase chain reaction genes involved RAS/cAMP/PKA signaling pathway play an important role activity. cytotoxic studies, inhibited only 12% growth human embryonic kidney cell line 293 cells, indicating nanocomposites not cytotoxic. Thus, produced by P. can be used as innovative effective drug carriers to increase activity nystatin.

Language: Английский

In Vitro Assessment of Antifungal and Antibiofilm Efficacy of Commercial Mouthwashes against Candida albicans DOI Creative Commons

Marzena Korbecka-Paczkowska,

Tomasz M. Karpiński

Antibiotics, Journal Year: 2024, Volume and Issue: 13(2), P. 117 - 117

Published: Jan. 25, 2024

Candida albicans is the most critical fungus causing oral mycosis. Many mouthwashes contain antimicrobial substances, including antifungal agents. This study aimed to investigate in vitro activity of 15 commercial against 12 strains C. albicans. The minimal inhibitory concentrations (MICs), fungicidal (MFCs), and anti-biofilm were studied. MICs determined by micro-dilution method using 96-well plates, MFCs culturing MIC suspensions on Sabouraud dextrose agar. Anti-biofilm was evaluated crystal violet method. containing octenidine dihydrochloride (OCT; mean 0.09–0.1%), chlorhexidine digluconate (CHX; 0.12%), CHX with cetylpyridinium chloride (CPC; 0.13%) exhibited best active compound 0.5–0.9 µg/mL for OCT products 1.1–2.4 rinses. For + CPC, 1.56 0.65 µg/mL, respectively. Products polyaminopropyl biguanide (polyhexanide, PHMB; 1.89%) or benzalkonium (BAC; 6.38%) also showed good anti-Candida action. In biofilm reduction studies, demonstrated substantial effect (47–51.1%). (32.1–41.7%), PHMB (38.6%), BAC (35.7%), Scutellaria extract (35.6%), fluorides essential oils (33.2%) moderate antibiofilm activity. paper provides an overview side effects CHX, OCT. Considering albicans, it can be inferred that, clinically, are likely offer highest effectiveness. Meanwhile, PHMB, considered as promising alternatives.

Language: Английский

Citations

15
Zinc oxide nanoparticles functionalized with cinnamic acid for targeting dental pathogens receptor and modulating apoptotic genes in human oral epidermal carcinoma KB cells DOI

O V Ravikumar,

Vanitha Marunganathan,

Meenakshi Sundaram Kishore Kumar

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: Feb. 24, 2024

Language: Английский

Citations

15
Piperine-coated zinc oxide nanoparticles target biofilms and induce oral cancer apoptosis via BCl-2/BAX/P53 pathway DOI Creative Commons
Mohammed Rafi Shaik,

Karthikeyan Kandaswamy,

Ajay Guru

et al.

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: June 21, 2024

Abstract Background Dental pathogens play a crucial role in oral health issues, including tooth decay, gum disease, and infections, recent research suggests link between these cancer initiation progression. Innovative therapeutic approaches are needed due to antibiotic resistance concerns treatment limitations. Methods We synthesized analyzed piperine-coated zinc oxide nanoparticles (ZnO-PIP NPs) using UV spectroscopy, SEM, XRD, FTIR, EDAX. Antioxidant antimicrobial effectiveness were evaluated through DPPH, ABTS, MIC assays, while the anticancer properties assessed on KB squamous carcinoma cells. Results ZnO-PIP NPs exhibited significant antioxidant activity of 50 µg/mL against dental pathogens, indicating strong properties. Interaction analysis revealed high binding affinity with pathogens. showed dose-dependent cells, upregulating apoptotic genes BCL2, BAX, P53. Conclusions This approach offers multifaceted solution combatting both infections cancer, showcasing their potential for advancement healthcare. It is essential acknowledge limitations challenges associated use ZnO clinical applications. These may include regarding nanoparticle toxicity, biocompatibility, long-term safety. Further rigorous testing warranted address issues ensure safe effective translation into practice.

Language: Английский

Citations

15
Antifungal drug resistance in Candida: a special emphasis on amphotericin B DOI

Lailema Ahmady,

Manisha Gothwal,

Muhammed Mushthaque Mukkoli

et al.

Apmis, Journal Year: 2024, Volume and Issue: 132(5), P. 291 - 316

Published: March 11, 2024

Invasive fungal infections in humans caused by several Candida species, increased considerably immunocompromised or critically ill patients, resulting substantial morbidity and mortality. albicans is the most prevalent although frequency of these organisms varies greatly according to geographic region. Infections with C. non‐albicans species have become more common, especially past 20 years, as a result aging, immunosuppressive medication use, endocrine disorders, malnourishment, extended use medical equipment, an increase immunogenic diseases. Despite being frequently associated human infections, glabrata , parapsilosis tropicalis krusei also been identified. Several antifungal drugs different modes action are approved for clinical settings treat infections. However, due common eukaryotic structure fungi, only limited number available therapeutic use. Furthermore, drug resistance has emerged growing currently against Amphotericin B (AmB), polyene class drugs, mainly used treatment serious systemic AmB interacts plasma membrane ergosterol, triggering cellular ion leakage via pore formation, extracting ergosterol from inducing death. primarily changes content ergosterol. This review summarizes exhibited special focus on AmB.

Language: Английский

Citations

13
Mood and microbes: a comprehensive review of intestinal microbiota’s impact on depression DOI Creative Commons

Ameer Luqman,

Mei He, Adil Hassan

et al.

Frontiers in Psychiatry, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 9, 2024

Depression is considered a multifaceted and intricate mental disorder of growing concern due to its significant impact on global health issues. The human gut microbiota, also known as the “second brain,” has an important role in CNS by regulating it through chemical, immunological, hormonal, neurological processes. Various studies have found bidirectional link between brain gut, emphasizing onset depression therapies. biological molecular processes underlying microbiota are required, association may represent novel study. However, profound insights into stratification diversity still uncommon. This article investigates emerging evidence bacterial relationship brain’s system potential pathogenicity relevance. interplay immune system, nervous neurotransmitter synthesis, neuroplasticity transitions widely studied. consequences stress, dietary fibers, probiotics, prebiotics, antibiotics GB axis being Multiple revealed this led development effective microbiota-based drugs for both prevention treatment. Therefore, results support hypothesis that influences provide promising area research improved knowledge etiology disease future

Language: Английский

Citations

11
Fungal coexistence in the skin mycobiome: a study involving Malassezia, Candida, and Rhodotorula DOI Creative Commons

Bharati Naik,

Jayaprakash Sasikumar,

B Vishal

et al.

AMB Express, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 20, 2024

Abstract Evidence of fungal coexistence in humans points towards adaptation to the host environment, like skin. The human commensal Malassezia has evolved, especially residing sebum-rich areas mammalian body where it can get necessary nutrition for its survival. This fungus is primarily responsible skin diseases Pityriasis versicolor (PV), characterized by hypo or hyperpigmented discoloration and erythematous macules. In this manuscript, we report a 19-year-old healthy female who presented with one-year history reddish, hypopigmented, asymptomatic lesions over chest raised lesion face. Upon clinical observation, patient displayed multiple macules papules bilateral malar area face, along hypopigmented scaly present on back. Based above findings, diagnosis PV Acne vulgaris (AV) was made. Interestingly, immunocompetent didn’t have any comorbidities. isolation scrapings post-culturing, found existence three genera same region patient’s body. We further went confirm identity particular species represent Malassezia, Rhodotorula , Candida . how predominant microbial resident fungus, coexists other members mycobiome. study an applied aspect microbiology also shows important identify organism associated infections so that appropriate therapeutics be advised avoid cases relapse.

Language: Английский

Citations

8
Cadmium-induced augmentation of fungal translocation promotes systemic infection in mice via gut barrier disruption and immune dysfunction DOI

Manika Garg,

Muskan Verma,

Aiysha Siddiq Khan

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: 362, P. 123368 - 123368

Published: Jan. 4, 2025

Language: Английский

Citations

1
Lanosterol 14α-Demethylase (CYP51)/Heat Shock Protein 90 (Hsp90) Dual Inhibitors for the Treatment of Invasive Candidiasis DOI
Mingming Zhang,

Wanzhen Yang,

Na Liu

et al.

Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 4, 2025

Invasive candidiasis has attracted global attention with a high incidence and mortality. Current antifungal drugs are limited by unfavorable therapeutic efficacy, significant hepatorenal toxicity, the development of drug resistance. Herein, we designed first generation lanosterol 14α-demethylase (CYP51)/heat shock protein 90 (Hsp90) dual inhibitors on basis synergism. Among them, inhibitor

Language: Английский

Citations

1
Synthesis, Antifungal Activity, Molecular Docking and DFT Analysis of New (E)-4-(4-Aryl)-2-(2-(1-(2,4-dimethoxyphenyl)ethylidene)hydrazineyl)thiazoles: An Integrated Experimental and Theoretical Study DOI

S. R. LOKHANDE,

Vishnu A. Adole, R. Rajesh

et al.

Journal of Molecular Structure, Journal Year: 2025, Volume and Issue: unknown, P. 141761 - 141761

Published: Feb. 1, 2025

Language: Английский

Citations

1
Evaluation of the Disinfection Efficacy of Er: YAG Laser Light on Single-Species Candida Biofilms—An In Vitro Study DOI Creative Commons

Diana Dembicka-Mączka,

Małgorzata Kępa, Jakub Fiegler-Rudol

et al.

Dentistry Journal, Journal Year: 2025, Volume and Issue: 13(2), P. 88 - 88

Published: Feb. 19, 2025

Background/Objectives: Oral candidiasis is an opportunistic infection caused by Candida species. Recently, antifungal drugs have become less effective due to yeast resistance, emphasizing the need for new treatment strategies. This study aimed assess effect of Er:YAG laser on inhibition growth and elimination mature single-species biofilms. Methods: The utilized reference strains C. albicans, glabrata, parapsilosis, krusei organized in biofilms Sabouraud dextrose agar (SDA). First part: suspensions (0.5 McFarland standard) were spread SDA plates-two each strain. irradiation was applied a single pulse mode, 30 400 mJ, 32 predetermined points. zones (GIZs) measured at 24-96 h incubation. Second prepared similarly and, after 96 incubation, exposed different energies (50, 100, 150, 200 mJ) 180 s, per 1.44 cm area. Post-irradiation, impressions taken using Rodac Agar determine counts. count colony-forming units (CFU) results analysed statistically. Results: GIZ found all irradiated sites, with various strains. showed significant increase width energy range 30-280 mJ non-significant 300-400 mJ. number CFU remaining 150 differed statistically significantly from other obtained 50, or energy, regardless strain tested. Conclusions: shown good disinfecting properties (inhibiting biofilm growth, even low-energy doses (50 mJ), eliminating maturity, spp. most dose).

Language: Английский

Citations

1