IMA Fungus, Journal Year: 2024, Volume and Issue: 15(1)
Published: Sept. 9, 2024
Language: Английский
Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)
Published: Feb. 14, 2023
Nematode-trapping (NT) fungi are a unique group of carnivorous microorganisms that can capture and digest nematodes by producing ingenious trapping devices (traps).
Language: Английский
Citations
17
Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown
Published: Feb. 1, 2024
Arthrobotrys oligospora has been utilized as a model strain to study the interaction between fungi and nematodes owing its ability capture by developing specialized traps. A previous showed that high-osmolarity glycerol (Hog1) signaling regulates osmoregulation nematocidal activity of A. oligospora. However, function downstream transcription factors Hog1 in nematode-trapping (NT) remains unclear. This aimed investigate functions potential regulatory network AoMsn2, factor pathway The AoMsn2 was characterized using targeted gene deletion, phenotypic experiments, real-time quantitative PCR, RNA sequencing, untargeted metabolomics, yeast two-hybrid analysis. Loss Aomsn2 significantly enlarged swollen hyphae, with an increase septa significant decrease nuclei. In particular, spore yield, germination rate, traps, nematode predation efficiency were remarkably decreased mutants. Phenotypic transcriptomic analyses revealed is essential for fatty acid metabolism autophagic pathways. Additionally, metabolomic analysis identified important modulation secondary metabolites. Furtherly, we analyzed protein based on Kyoto Encyclopedia Genes Genomes map online website STRING. Finally, six putative proteins Y2H Our reveals plays crucial roles growth, conidiation, trap development, metabolism, well establishes broad basis understanding mechanisms morphogenesis environmental adaptation NT fungi.
Language: Английский
Citations
6
Microbiological Research, Journal Year: 2023, Volume and Issue: 280, P. 127573 - 127573
Published: Dec. 12, 2023
Language: Английский
Citations
9
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Feb. 11, 2025
Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) facilitate intracellular vesicle trafficking and membrane fusion in eukaryotic organisms, significantly influencing fungal growth, development, pathogenicity. Despite their importance, the specific functions of individual SNARE subunits remain largely unexplored. In Cytospora chrysosperma, a pathogen causing substantial damage to woody plants leading considerable ecological economic impacts, 22 SNAREs have been identified. Yet, functional roles not previously characterized. this study, we focused on two SNARE-encoding genes, CcSec22 CcSso1 that are supposed mediate endoplasmic reticulum (ER)-Golgi trans-Golgi Network (TGN)-secretory transport respectively. Notably, both genes demonstrate elevated expression during infection process. Targeted deletion either them resulted retarded mycelial growth remarkably decreased tolerance towards different cell-wall stressors. Interestingly, mutants formed more hyphal branches while was required for morphological maintenance conidiospores. More importantly, lack reduced endocytosis virulence host poplar. Collectively, our data highlighted play pleiotropic stress responses, conidiation, particular, they full C. chrysosperma. This study provides an insight into role proteins chrysosperma pathogenesis.
Language: Английский
Citations
0
Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(4), P. 295 - 295
Published: April 9, 2025
Bursaphelenchus xylophilus, the causative agent of pine wilt disease (PWD), poses a severe global threat to coniferous forests. Esteya vermicola, an endoparasitic nematophagous fungus, exhibits promising biocontrol potential against this pinewood nematode. The vesicular transport system, evolutionarily conserved in eukaryotes, is essential for fungal pathogenicity. Based on our genome sequence E. vermicola CBS115803, we identified EvSec22, gene encoding SNARE protein implicated process. This study investigates role EvSec22 during nematode infection, utilizing optimized knockout methodology. Infection assays revealed that deletion significantly impaired pathogenicity B. xylophilus. Phenotypic analyses ΔEvSec22 mutant exhibited suppressed hyphal growth, reduced conidiation, and abnormal septal spacing. Furthermore, showed diminished tolerance osmotic stress (sorbitol) oxidative (hydrogen peroxide). Overall, associated with virulence CBS115803 its also affects normal growth abiotic stress. providing new insights into functions agents.
Language: Английский
Citations
0
Microbiological Research, Journal Year: 2023, Volume and Issue: 278, P. 127516 - 127516
Published: Oct. 12, 2023
Language: Английский
Citations
8
Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(4), P. 496 - 496
Published: April 21, 2023
Malate dehydrogenase (MDH) is a key enzyme in the tricarboxylic acid (TCA) cycle and essential for energy balance, growth, tolerance to cold salt stresses plants. However, role of MDH filamentous fungi still largely unknown. In this study, we characterized an ortholog (AoMae1) representative nematode-trapping (NT) fungus Arthrobotrys oligospora via gene disruption, phenotypic analysis, nontargeted metabolomics. We found that loss Aomae1 led weakening activity ATP content, remarkable decrease conidia yield, considerable increase number traps mycelial loops. addition, absence also caused obvious reduction septa nuclei. particular, AoMae1 regulates hyphal fusion under low nutrient conditions but not nutrient-rich conditions, volumes sizes lipid droplets dynamically changed during trap formation nematode predation. involved regulation secondary metabolites such as arthrobotrisins. These results suggest has important fusion, sporulation, production, formation, pathogenicity A. oligospora. Our enhance understanding crucial enzymes TCA play development, NT fungi.
Language: Английский
Citations
6
Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(2), P. 110 - 110
Published: Jan. 28, 2024
Prdx2 is a peroxiredoxin (Prx) family protein that protects cells from attack via reactive oxygen species (ROS), and it has an important role in improving the resistance scavenging capacity of ROS fungi. Arthrobotrys oligospora widespread nematode-trapping fungus can produce three-dimensional nets to capture kill nematodes. In this study, AoPrdx2, homologous Prx5, was investigated A. gene disruption, phenotypic analysis, metabolomics. The deletion Aoprdx2 resulted increase number mycelial septa reduction nuclei spore yield. Meanwhile, absence increased sensitivity oxidative stresses, whereas ∆Aoprdx2 mutant strain higher levels than wild-type (WT) strain. particular, inactivation severely influenced trap formation pathogenicity; traps produced by remarkably reduced rings less WT addition, abundance metabolites significantly downregulated compared with These results indicate AoPrdx2 plays indispensable ROS, morphogenesis, secondary metabolism.
Language: Английский
Citations
1
Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14
Published: Sept. 14, 2023
The p21-GTPase-activated protein kinases (PAKs) participate in signal transduction downstream of Rho GTPases, which are regulated by GTPase-activating proteins (Rho-GAP). Herein, we characterized two orthologous Rho-GAPs (AoRga1 and AoRga2) PAKs (AoPak1 AoPak2) through bioinformatics analysis reverse genetics Arthrobotrys oligospora, a typical nematode-trapping (NT) fungus. transcription analyses performed at different development stages suggested that Aopaks Aorga1 play crucial role during sporulation trap formation, respectively. In addition, successfully deleted Aopak1 via the homologous recombination method. disruption caused remarkable reduction spore yield number nuclei per cell, but did not affect mycelial growth. ∆Aopak1 mutants, was decreased 48 h after introduction nematodes, nematode predatory efficiency affected because extracellular proteolytic activity increased. On contrary, traps ∆Aorga1 mutants significantly increased 36 h. had effects on sensitivity to cell-wall-disturbing reagent oxidant. A yeast two-hybrid assay revealed AoPak1 AoRga1 both interacted with AoRac, also AoCdc42. Furthermore, were up-regulated down-regulated mutants. These results reveal indirectly AoPAKs regulating small GTPases.
Language: Английский
Citations
3
Microorganisms, Journal Year: 2023, Volume and Issue: 11(6), P. 1612 - 1612
Published: June 19, 2023
Multidrug resistance (Mdr) proteins are critical for maintenance of drug in fungi. Mdr1 has been extensively studied Candida albicans; its role other fungi is largely unknown. In this study, we identified a homologous protein Mdr (AoMdr1) the nematode-trapping (NT) fungus Arthrobotrys oligospora. It was found that deletion Aomdr1 resulted significant reduction number hyphal septa and nuclei as well increased sensitivity to fluconazole hyperosmotic stress SDS. The also led remarkable increase numbers traps mycelial loops traps. Notably, AoMdr1 able regulate fusion under low-nutrient conditions, but not nutrient-rich conditions. involved secondary metabolism, caused an arthrobotrisins (specific compounds produced by NT fungi). These results suggest plays crucial resistance, fusion, conidiation, trap formation, metabolism A. Our study contributes understanding growth development
Language: Английский
Citations
1