TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118190 - 118190
Published: Feb. 1, 2025
Language: Английский
Cancers, Journal Year: 2022, Volume and Issue: 14(13), P. 3272 - 3272
Published: July 4, 2022
Although appreciable attempts in screening and diagnostic approaches have been achieved, prostate cancer (PCa) remains a widespread malignancy, representing the second leading cause of cancer-related death men. Drugs currently used PCa therapy initially show potent anti-tumor effect, but frequently induce resistance progresses toward metastatic castration-resistant forms (mCRPC), virtually incurable. Liquid biopsy has emerged as an attractive promising strategy complementary to invasive tissue guide diagnosis treatment. shows ability represent tumor microenvironment, allow comprehensive information follow-up progression tumor, enabling development different treatment strategies well permitting monitoring response. biopsy, indeed, is endowed with significant potential modify management. Several blood biomarkers could be analyzed for diagnostic, prognostic predictive purposes, including circulating cells (CTCs), extracellular vesicles (EVs), DNA (ctDNA) RNA (ctRNA). In addition, several other body fluids may adopted (i.e., urine, sperm, etc.) beyond blood. This review dissects recent advancements future perspectives liquid biopsies, highlighting their strength weaknesses
Language: Английский
Citations
78
Critical Reviews in Oncology/Hematology, Journal Year: 2022, Volume and Issue: 170, P. 103577 - 103577
Published: Jan. 5, 2022
Language: Английский
Citations
70
International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(8), P. 4173 - 4173
Published: April 10, 2022
Metabolomic analysis has proven to be a useful tool in biomarker discovery and the molecular classification of cancers. In order find new biomarkers, better understand its pathological behavior, bladder cancer also been studied using metabolomics approach. this article, we review literature on metabolomic studies cancer, focusing different available samples (urine, blood, tissue samples) used perform their relative findings. Moreover, multi-omic approach research found novel insights into metabolic providing excellent start-points for diagnostic therapeutic strategies. Metabolomics data can lead “signature pathway” associated with progression cancer; aspect could potentially valuable predictions clinical outcomes introduction treatments. However, further are needed give stronger evidence make these tools feasible use practice.
Language: Английский
Citations
52
International Journal of Cancer, Journal Year: 2023, Volume and Issue: 152(10), P. 2186 - 2205
Published: Jan. 17, 2023
Abstract The aim of this review was to explore the status urine sampling as a liquid biopsy for noninvasive cancer research by reviewing used preanalytical parameters and protocols. We searched two main health sciences databases, PubMed Web Science. From all eligible publications (2010‐2022), information extracted regarding: (a) study population characteristics, (b) type, (c) preanalytics, (d) analyte class, (e) isolation method, (f) detection (g) comparator used, (h) biomarker (i) conclusion (j) sensitivity specificity. search query identified 7835 records, which 924 unique remained after screening title, abstract full text. Our analysis demonstrated that many did not report about their samples, even though several other studies have shown importance standardization sample handling. Interestingly, it noted is types just cancers originating from urogenital tract. Many different relevant analytes been be found in urine. Additionally, future considerations recommendations are discussed: heterogeneous nature urine, need standardized practice protocols road toward clinic. Urine an emerging with broad applicability types. However, standard handling processing would help elaborate clinical utility research, disease monitoring.
Language: Английский
Citations
30
Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 248, P. 154632 - 154632
Published: June 20, 2023
Language: Английский
Citations
27
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Feb. 8, 2025
Bladder cancer (BLCA) is one of the ten most common cancers worldwide. However, deregulation PROS1 and its specific function in BLCA not well understood. By combining proteomic transcriptomic datasets, we discovered expression was significantly reduced tissues revealed clinical relevance with BLCA. Analysis multiple datasets consistently showed group linked to cancer-promoting pathways, more aggressive characteristics, a greater chance responding positively immunotherapy. Next, various functional experiments were performed results overexpression inhibited proliferation, cell cycle progression, migration, invasion, angiogenesis In recovery trials, AKT activator SC79 offered additional proof that may influence cells via AKT/GSK3β/β-catenin pathway. conclusion, as an angiogenesis-related gene, play inhibitory role biological functions bladder cancer.
Language: Английский
Citations
1
Cellular & Molecular Biology Letters, Journal Year: 2023, Volume and Issue: 28(1)
Published: April 4, 2023
Abstract Bladder cancer (BC) is a clinical challenge worldwide with late presentation, poor prognosis, and low survival rates. Traditional cystoscopy tissue biopsy are routine methods for the diagnosis, monitoring of BC. However, due to heterogeneity limitations tumors, such as aggressiveness, high cost, limited applicability longitudinal surveillance, identification tumor markers has attracted significant attention in Over past decade, liquid biopsies (e.g., blood) have proven be highly efficient discovery BC biomarkers. This noninvasive sampling method used analyze unique components released into peripheral circulation allows serial progression. Several biomarkers being extensively studied shown promising results applications BC, including early detection, detection microscopic residual disease, prediction recurrence, response therapy. Therefore, this review, we aim provide an update on various novel blood-based review advantages current The role circulating cells, DNA, cell-free RNA, exosomes, metabolomics, proteomics treatment monitoring, their personalized management highlighted.
Language: Английский
Citations
19
ACS Nano, Journal Year: 2023, Volume and Issue: 17(23), P. 23924 - 23935
Published: Dec. 1, 2023
Exosome metabolite-based noninvasive liquid biopsy is an emerging research hotspot that tends to substitute current means in clinics. Nanostructure-based mass spectrometry enables continuous exosome isolation and metabolic profiling with superior analysis speed high efficiency. Herein, we construct a heterogeneous MXene hybrid possesses ternary binding sites for capture outstanding matrix performance metabolite analysis. Upon optimizing experimental conditions, the average extraction of exosomes their patterns from 60 mL urine sample completed within 45 s (40 samples per batch 30 min). According exosomal subsequently established biomarker panel, distinguish early bladder cancer (BCa) healthy controls area under curve (AUC) value greater than 0.995 model training validation sets. As well, realize subtype classification BCa blind test on patterns, AUC 0.867. We also explore significant biomarkers are sensitive follow-up patients, which indeed present reverse change levels compared pathological progression. This study has potential guide development approach.
Language: Английский
Citations
17
Future Journal of Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 10(1)
Published: Jan. 5, 2024
Abstract Background Personalized or Precision medicine (PM) is a promising approach for the cancer treatment that tailors to patient's characteristics. Biomarkers are crucial identifying patients who expected derive greatest advantage from targeted therapy. Main body Here, various biomarkers, including genetic, epigenetic, protein, and metabolites, their clinical significance, discussed. The review provides insights into use of biomarkers significance in treatment. There several hurdles PM oncology, such as complexity tumor biology heterogeneity, limited availability high cost therapies, resistance ethical social issues. Conclusion play diagnostic role cancer. also acknowledges challenges limitations personalized which, if resolved, can be helpful management
Language: Английский
Citations
8
Diagnostics, Journal Year: 2022, Volume and Issue: 12(2), P. 431 - 431
Published: Feb. 7, 2022
Lipid alterations may serve as potential tumour biomarkers. The ratio of triglycerides to HDL cholesterol (TG/HDL ratio) is associated with various cancers. Pseudocholinesterase (PChE) activity, involved in TG hydrolysis, plays an important role the metabolism lipoprotein. There scarce data assessing reliability both TG/HDL and PChE levels correctly classifying patients suffering from bladder cancer.Three hundred ninety-six undergoing cystoscopy or transurethral resection (TURB), broken into two major groups, i.e., histologically confirmed, non-metastatic cancer (n = 208) without (no cancer, n 188), formed study population. last group was split subgroups consisting a cohort never but other diseases CaBD, 100) another by characterised eradicated after TURB no recurrence during three-month follow-up (previous 88). Pieces information metabolic derangement (the presence type 2 diabetes mellitus), hypertension lipid profile were retrieved patient records upon entry study. Sensitivity, specificity, areas under ROC (AUROC) ratio, used diagnostic decision making.The well concentrations significantly different when compared those previous CaBD (p 0.023 0.0004, respectively). independent predicting (OR: 1.22 0.99, respectively), (AUROC: 0.587) superior that 0.374). AUROC new parameter resulting combination showed further increment discriminant power (0.6298), interestingly negative predictive value (89%) according Bayesian approach. cut-off main marker present better distinguishes patients, 2.147.The based on fact this likely mirrors insulin resistance (IR) underlying patients. Furthermore, evidence IR non-alcoholic fatty liver disease. their combined use could help physicians assess/confirm very common where early detection ensure best therapeutical
Language: Английский
Citations
28