Magnetofluid-integrated biosensors based on DNase-dead Cas12a for visual point-of-care testing of HIV-1 by an up and down chip DOI
Di Huang,

Yekai Zhao,

Mengjun Fang

et al.

Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(19), P. 4265 - 4275

Published: Jan. 1, 2023

The CRISPR Cas system, as a novel nucleic acid detection tool, is often hindered by cumbersome experimental procedures, complicated reagent transfer processes, and associated aerosol pollution risks. In this study, an integrated platform named "up down chip" was developed, which combined RT-RAA technology for amplification, DNase-dead Cas12a-modified magnetic beads specific recognition of target acid, HRP-TMB chromogenic reaction signal output in different chambers single microfluidic chip. were migrated up-and-down manner between through driving, achieving "sample-in, result-out" mode. By introducing homemade heating box temperature control during the using naked eye or smartphone APP color-based reading, no professional precise instruments required platform. Using platform, highly sensitive HIV-1 genome low 250 copies (CPs) per mL achieved within 100 min while maintaining good performance against common variants well excellent specificity anti-interference ability. addition, compared with qRT-PCR, it also exhibited accuracy 56 spiked plasma samples, indicating its promising potential clinical application.

Language: Английский

Mutations in SARS-CoV-2: Insights on structure, variants, vaccines, and biomedical interventions DOI Open Access
Ahmed I. Abulsoud,

Hussein M. El‐Husseiny,

Ahmed A. El-Husseiny

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2022, Volume and Issue: 157, P. 113977 - 113977

Published: Nov. 7, 2022

COVID-19 is a worldwide pandemic caused by SARS-coronavirus-2 (SARS-CoV-2). Less than year after the emergence of Covid-19 pandemic, many vaccines have arrived on market with innovative technologies in field vaccinology. Based use messenger RNA (mRNA) encoding Spike SARS-Cov-2 protein or recombinant adenovirus vectors enabling gene to be introduced into our cells, these strategies make it possible envisage vaccination new light tools that are more scalable vaccine used so far. Faced appearance variants, which will gradually take precedence over strain at origin allow much faster update fight against some may escape neutralization antibodies. However, only policy based rapid and massive population but requiring supply sufficient doses could combat variants. Indeed, greater number infected individuals, virus multiplies, an increased risk variants viruses. This review discuss SARS-CoV-2 pathophysiology evolution approaches altered transmission platforms emphasize different mutations how they influence characteristics. Also, this article summarizes common implication genetic variety biomedical arbitrations.

Language: Английский

Citations

90
WS2/Si3N4-Based Biosensor for Low-Concentration Coronavirus Detection DOI Creative Commons
Talía Tene, Fabian Arias Arias, Karina I. Paredes-Páliz

et al.

Micromachines, Journal Year: 2025, Volume and Issue: 16(2), P. 128 - 128

Published: Jan. 23, 2025

This study presents the optimization of two SPR biosensors, Sys3 and Sys5, for SARS-CoV-2 detection at concentrations 0.01-100 nM. Sys3, with a 55 nm silver layer, 13 silicon nitride 10 ssDNA achieved figure merit (FoM) 571.24 RIU-1, signal-to-noise ratio (SNR) 0.12, accuracy (DA) 48.93 × 10-2. incorporating 50 1.6 tungsten disulfide layer (L = 2), demonstrated higher sensitivity 305.33 °/RIU lower limit (LoD) 1.65 10-5. outshined in precision low attenuation (<1%), while Sys5 provided enhanced limits, crucial early-stage viral detection. These configurations align refractive index ranges clinical samples, showcasing their diagnostic potential. Future work will focus on experimental validation integration into point-of-care platforms.

Language: Английский

Citations

2
Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics DOI Open Access
Zheng Li, Xiaojian Xu, Dou Wang

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2022, Volume and Issue: 158, P. 116871 - 116871

Published: Dec. 5, 2022

Language: Английский

Citations

56
Advanced Molecular and Immunological Diagnostic Methods to Detect SARS-CoV-2 Infection DOI Creative Commons
John Charles Rotondo, Fernanda Martini, Martina Maritati

et al.

Microorganisms, Journal Year: 2022, Volume and Issue: 10(6), P. 1193 - 1193

Published: June 10, 2022

COVID-19 emerged in late 2019 China and quickly spread across the globe, causing over 521 million cases of infection 6.26 deaths to date. After 2 years, numerous advances have been made. First all, preventive vaccine, which has implemented record time, is effective more than 95% cases. Additionally, diagnostic field, there are molecular antigenic kits that equipped with high sensitivity specificity. Real Time-PCR-based assays for detection viral RNA currently considered gold-standard method SARS-CoV-2 diagnosis can be used efficiently on pooled nasopharyngeal, or oropharyngeal samples widespread screening. Moreover, additional, advanced methods such as droplet-digital PCR (ddPCR), clustered regularly interspaced short palindromic repeats (CRISPR) next-generation sequencing (NGS), under development detect RNA. However, number subjects infected continuously increases globally, health care systems being placed increased stress. Thus, clinical laboratory plays an important role, helping select especially asymptomatic individuals who actively carrying live replicating virus, fast non-invasive technologies. Recent strategies, other methods, adopted either antigens, i.e., antigen-based immunoassays, human anti-SARS-CoV-2 antibodies, antibody-based nasal swabs, well blood saliva samples. role mucosal sIgAs, essential control viruses entering body through surfaces, remains elucidated, particular immune response counteracting infection, primarily at site(s) virus entry appears promising.

Language: Английский

Citations

53
COVID‐19: A systematic review and update on prevention, diagnosis, and treatment DOI
Hooman Aghamirza Moghim Aliabadi,

Reza Eivazzadeh‐Keihan,

Arezoo Beig Parikhani

et al.

MedComm, Journal Year: 2022, Volume and Issue: 3(1)

Published: Feb. 17, 2022

Abstract Since the rapid onset of COVID‐19 or SARS‐CoV‐2 pandemic in world 2019, extensive studies have been conducted to unveil behavior and emission pattern virus order determine best ways diagnosis thereof formulate effective drugs vaccines combat disease. The emergence novel diagnostic therapeutic techniques considering multiplicity reports from one side contradictions assessments other necessitates instantaneous updates on progress clinical investigations. There is also growing public anxiety time mutation COVID‐19, as reflected considerable mortality transmission, respectively, delta Omicron variants. We comprehensively review summarize different aspects prevention, diagnosis, treatment COVID‐19. First, biological characteristics were explained standpoint. Thereafter, preclinical animal models discussed frame symptoms effects patient with strategies in‐silico/computational biology. Finally, opportunities challenges nanoscience/nanotechnology identification, discussed. This covers almost all SARS‐CoV‐2‐related topics extensively deepen understanding latest achievements (last updated January 11, 2022).

Language: Английский

Citations

44
CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives DOI Creative Commons
Tao Huang, Rui Zhang, Jinming Li

et al.

Journal of Advanced Research, Journal Year: 2022, Volume and Issue: 50, P. 69 - 82

Published: Oct. 30, 2022

Early detection of pathogen-associated diseases are critical for effective treatment. Rapid, specific, sensitive, and cost-effective diagnostic technologies continue to be challenging develop. The current gold standard pathogen detection, polymerase chain reaction technology, has limitations such as long operational cycles, high cost, technician instrumentation requirements.This review examines highlights the technical advancements CRISPR-Cas in provides an outlook future development, multi-application scenarios, clinical translation.Approaches enabling nucleic acids that highly cheap, portable necessary. CRISPR-Cas9 specificity targeting "collateral cleavage" activity CRISPR-Cas12/Cas13/Cas14 show significant promise acid technology. These methods have a specificity, versatility, rapid cycle. In this paper, CRISPR-Cas-based discussed depth. Although CRISPR-Cas-mediated solutions face challenges, their powerful capabilities will pave way ideal tools.

Language: Английский

Citations

40
Metal/covalent-organic framework-based biosensors for nucleic acid detection DOI

Zi-Wei Yang,

Jiajing Li, Yuhan Wang

et al.

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 491, P. 215249 - 215249

Published: May 30, 2023

Language: Английский

Citations

26
Digital PCR for the characterization of reference materials DOI Creative Commons
Megan H. Cleveland, Hua‐Jun He, Mojca Milavec

et al.

Molecular Aspects of Medicine, Journal Year: 2024, Volume and Issue: 96, P. 101256 - 101256

Published: Feb. 14, 2024

Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific acid sequence in background non-target sequences, making it ideal for characterization materials. National Metrology Institutes are increasingly using dPCR to characterize certify their materials, offers several advantages over indirect methods, such UV-spectroscopy. While is gaining widespread adoption, requires optimization has certain limitations considerations that users should be aware characterizing This review highlights technical dPCR, well its role developing

Language: Английский

Citations

9
Microfluidic immunoassays for point-of-care testing of SARS-CoV-2 antigens and antibodies DOI

Cuili Li,

Wan Zhou,

Angel Gutierrez Ruiz

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 177, P. 117809 - 117809

Published: June 6, 2024

Language: Английский

Citations

9
Immunocapture Magnetic Beads Enhanced the LAMP-CRISPR/Cas12a Method for the Sensitive, Specific, and Visual Detection of Campylobacter jejuni DOI Creative Commons
Chao Li, Xuan Chen, Renqiao Wen

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 12(3), P. 154 - 154

Published: March 2, 2022

Campylobacter jejuni is one of the most important causes food-borne infectious disease, and poses challenges to food safety public health. Establishing a rapid, accurate, sensitive, simple detection method for C. enables early diagnosis, intervention, prevention pathogen transmission. In this study, an immunocapture magnetic bead (ICB)-enhanced loop-mediated isothermal amplification (LAMP) CRISPR/Cas12a (ICB-LAMP-CRISPR/Cas12a) was developed rapid visual jejuni. Using ICB-LAMP-CRISPR/Cas12a method, first captured by ICB, bacterial genomic DNA then released heating used in LAMP reaction. After reaction, products were mixed detected cleavage mixture. This could detect minimum 8 CFU/mL within 70 min. Additionally, performed closed tube addition ICB capture, which eliminates need separate preamplification transfer amplified avoid aerosol pollution. The further validated testing 31 jejuni-positive fecal samples from different layer farms. all-in-one, simple, ultrasensitive, ultraspecific, instrument-free diagnosis jejuni, has wide application potential future work.

Language: Английский

Citations

34