Host-microbe
interactions
are
virtually
bidirectional,
but
how
the
host
affects
their
microbiome
is
poorly
understood.
Here,
we
report
that
a
critical
modulator
to
regulate
lifestyle
switch
and
pathogenicity
heterogeneity
of
opportunistic
pathogens
Serratia
marcescens
utilizing
Drosophila
bacterium
model
system.
First,
find
larvae
efficiently
outcompete
S.
typically
drive
bacterial
from
commensalism
toward
fly.
Furthermore,
reshape
transcriptomic
metabolic
profiles
characterized
by
switch.
More
important,
alters
in
single-cell
resolution.
Finally,
larvae-derived
AMPs
required
recapitulate
response
larvae.
Altogether,
our
findings
provide
an
insight
into
pivotal
roles
harnessing
life
history
symbiotic
cells,
advancing
knowledge
reciprocal
relationships
between
pathogen.
Sensors and Actuators A Physical,
Journal Year:
2024,
Volume and Issue:
376, P. 115625 - 115625
Published: June 24, 2024
The
integration
of
CRISPR
technology
with
microfluidic-based
biosensors
has
greatly
expanded
its
applications
in
medicine
and
molecular
biology.
This
combination
offers
enhanced
sensitivity
selectivity
medical
diagnostics.
CRISPR-powered
microfluidics
enables
the
accurate
quantification
DNA
RNA
associated
diseases
such
as
cancer,
viral
infections,
bacterial
diseases.
precise
targeting
capabilities
allow
for
detection
specific
genetic
sequences,
aiding
early
diagnosis,
disease
monitoring,
treatment
assessment.
improves
specificity
by
leveraging
programmable
nature
coupling
it
signal
readouts.
However,
challenges
assay
optimization,
standardization,
device
fabrication
need
to
be
addressed.
Additionally,
complexity
presents
potential
limitations
terms
off-target
effects
unintended
modifications.
Overall,
holds
tremendous
diagnostics,
but
further
research
development
are
required
fully
exploit
benefits
clinical
settings.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(18), P. 14311 - 14311
Published: Sept. 20, 2023
The
recurrence
of
bacterial
infectious
diseases
is
closely
associated
with
persisters.
This
subpopulation
bacteria
can
escape
antibiotic
treatment
by
entering
a
metabolic
status
low
activity
through
various
mechanisms,
for
example,
biofilm,
toxin–antitoxin
modules,
the
stringent
response,
and
SOS
response.
Correspondingly,
multiple
new
treatments
are
being
developed.
However,
due
to
their
spontaneous
abundance
in
populations
lack
research
on
vivo
interactions
between
persisters
host’s
immune
system,
microfluidics,
high-throughput
sequencing,
microscopy
techniques
combined
innovatively
explore
mechanisms
persister
formation
maintenance
at
single-cell
level.
Here,
we
outline
main
formation,
describe
cutting-edge
technology
further
research.
Despite
significant
progress
regarding
study
techniques,
some
challenges
remain
be
tackled.
Antimicrobial Agents and Chemotherapy,
Journal Year:
2023,
Volume and Issue:
67(2)
Published: Jan. 10, 2023
Phenotypic
heterogeneity
is
crucial
to
bacterial
survival
and
could
provide
insights
into
the
mechanism
of
action
(MOA)
antibiotics,
especially
those
with
polypharmacological
actions.
Although
phenotypic
changes
among
individual
cells
be
detected
by
existing
profiling
methods,
due
data
complexity,
only
population
average
were
commonly
used,
thereby
overlooking
heterogeneity.
In
this
study,
we
developed
a
high-resolution
cytological
method
that
can
capture
morphological
variations
bacteria
upon
antibiotic
treatment.
With
an
unprecedented
single-cell
resolution,
classifies
known
MOAs
overall
accuracy
above
90%.
We
next
showed
combinations
two
antibiotics
induce
altered
cell
morphologies
are
either
unique
or
similar
in
combinations.
these
combinatorial
profiles,
successfully
revealed
multiple
caused
natural
product-derived
compound
that,
itself,
inactive
against
Acinetobacter
baumannii
but
synergistically
exerts
its
antibacterial
activities
presence
colistin.
The
findings
have
paved
way
for
future
highlighted
previously
underappreciated
intrapopulation
perturbation.
Host-microbe
interactions
are
virtually
bidirectional,
but
how
the
host
affects
their
microbiome
is
poorly
understood.
Here,
we
report
that
a
critical
modulator
to
regulate
lifestyle
switch
and
pathogenicity
heterogeneity
of
opportunistic
pathogens
Serratia
marcescens
utilizing
Drosophila
bacterium
model
system.
First,
find
larvae
efficiently
outcompete
S.
typically
drive
bacterial
from
commensalism
toward
fly.
Furthermore,
reshape
transcriptomic
metabolic
profiles
characterized
by
switch.
More
importantly,
alters
in
single-cell
resolution.
Finally,
larvae-derived
AMPs
required
recapitulate
response
larvae.
Altogether,
our
findings
provide
an
insight
into
pivotal
roles
harnessing
life
history
symbiotic
cells,
advancing
knowledge
reciprocal
relationships
between
pathogen.
Microsystems & Nanoengineering,
Journal Year:
2024,
Volume and Issue:
10(1)
Published: March 1, 2024
Abstract
Dielectrophoresis
is
a
powerful
and
well-established
technique
that
allows
label-free,
non-invasive
manipulation
of
cells
particles
by
leveraging
their
electrical
properties.
The
practical
implementation
the
associated
electronics
user
interface
in
biology
laboratory,
however,
requires
an
engineering
background,
thus
hindering
broader
adoption
technique.
In
order
to
address
these
challenges
bridge
gap
between
biologists
skills
required
for
DEP
platforms,
we
report
here
custom-built,
compact,
universal
electronic
platform
termed
ADEPT
(adaptable
dielectrophoresis
embedded
tool)
use
with
simple
microfluidic
chip
containing
six
microelectrodes.
versatility
open-source
ensured
custom-developed
graphical
permits
reconfiguration
control
signals
wide-range
specific
applications:
(i)
precision
positioning
single
bacterium/cell/particle
micrometer
range;
(ii)
viability-based
separation
achieving
94%
efficiency
separating
live
dead
yeast;
(iii)
phenotype-based
96%
yeast
Bacillus
subtilis
;
(iv)
cell–cell
interactions
steering
phagocytosis
process
where
granulocyte
engulfs
E.
coli
RGB-S
bacterium.
Together,
set
experiments
form
complete
basis
wide
range
possible
applications
addressing
various
biological
questions
exploiting
plug-and-play
design
intuitive
GUI
ADEPT.
