Liver Lipidomics Analysis Revealed the Novel Ameliorative Mechanisms of L-Carnitine on High-Fat Diet-Induced NAFLD Mice DOI Open Access

Chengyuan Sun,

Yan Guo,

Peixu Cong

et al.

Nutrients, Journal Year: 2023, Volume and Issue: 15(6), P. 1359 - 1359

Published: March 10, 2023

The beneficial effects of L-carnitine on non-alcoholic fatty liver disease (NAFLD) were revealed in previous reports. However, the underlying mechanisms remain unclear. In this study, we established a high fat diet (HFD)-induced NAFLD mice model and systematically explored dietary supplementation (0.2% to 4%) NAFLD. A lipidomics approach was conducted identify specific lipid species involved ameliorative roles Compared with normal control group, body weight, concentrations TG serum AST ALT levels dramatically increased by HFD feeding (p < 0.05), accompanied obvious damage activation hepatic TLR4/NF-κB/NLRP3 inflammatory pathway. treatment significantly improved these phenomena exhibited clear dose-response relationship. results analysis showed that total 12 classes 145 identified livers. Serious disorders profiles noticed livers HFD-fed mice, such as an relative abundance decreased PC, PE, PI, LPC, LPE, Cer SM 0.05). contents PC PI DG after 4% intervention Moreover, 47 important differential notably separated experimental groups based VIP ≥ 1 p 0.05. pathway inhibited glycerolipid metabolism activated pathways alpha-linolenic acid metabolism, glycerophospholipid sphingolipid Glycosylphosphatidylinositol (GPI)-anchor biosynthesis. This study provides novel insights into attenuating

Language: Английский

Lactobacillus plantarum attenuates glucocorticoid-induced osteoporosis by altering the composition of rat gut microbiota and serum metabolic profile DOI Creative Commons
Siying Li, Xuebing Han, Naiyuan Liu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 9, 2024

Introduction Osteoporosis, one of the most common non-communicable human diseases worldwide, is prevalent disease adult skeleton. Glucocorticoid-induced osteoporosis(GIOP) foremost form secondary osteoporosis, extensively researched due to its prevalence.Probiotics constitute a primary bioactive component within numerous foods, offering promise as potential biological intervention for preventing and treating osteoporosis. This study aimed evaluate beneficial effects probiotic Lactobacillus plantarum on bone health underlying mechanisms in rat model glucocorticoid dexamethasone-induced using osteoporosis treatment drug alendronate reference. Methods We examined microstructure (Micro-CT HE staining) analyzed gut microbiome serum metabolome rats. Results discussion The results revealed that L. significantly restored parameters microstructure, with elevated density, increased number thickness trabeculae, decreased Tb.Sp. Gut microbiota sequencing showed microbial diversity ratio Firmicutes Bacteroidota decreased. Beneficial bacteria abundance was ( Lachnospiraceae _NK4A136_group, Ruminococcus , UCG_005 Romboutsia Christensenellaceae _R_7_group), harmful Desulfovibrionaceae ). According metabolomics, significant changes metabolites occurred different groups. These differential were predominantly enriched pathways Pentose Glucuronate Interconversions, well Propanoate Metabolism. Furthermore, levels Pyrazine gamma-Glutamylcysteine, which associated inhibition osteoclast formation promoting osteoblast formation. can protect rats from DEX-induced GIOP by mediating “gut microbial-bone axis” production metabolites. Therefore candidate GIOP.

Language: Английский

Citations

16
Maternal consumption of l-malic acid enriched diets improves antioxidant capacity and glucose metabolism in offspring by regulating the gut microbiota DOI Creative Commons

Pengguang Zhang,

Guoyuan Jiang,

Yubo Wang

et al.

Redox Biology, Journal Year: 2023, Volume and Issue: 67, P. 102889 - 102889

Published: Sept. 19, 2023

Maternal diets during pregnancy and lactation are key determinants that regulate the development of metabolic syndrome (MetS) in offspring. l-malic acid (MA) was previously reported to improve antioxidant capacity aerobic metabolism. However, effects maternal MA consumption on features offspring remain largely unexplored. Herein, through pig models consuming MA-enriched late lactation, we found potentiated anti-inflammatory sows, thereby improving their reproductive performance growth piglets. also induced a transition slow-twitch fast-twitch fibers early life Along with muscle fiber-type transition, insulin sensitivity glucose metabolism, including metabolism glycolysis, were improved skeletal An untargeted metabolomic analysis further revealed contribution modified amino Mechanistically, remodeled colonic microbiota Briefly, abundance Colidextribacter, Romboutsia, Family_XIII_AD3011_group increased, which positively associated muscles. A decreased Prevotella, Blautia, Prevotellaceae_NK3B31_group, Collinsella detected, involved less sensitivity. Notably, milk metabolites, such as ascorbic (AA) granisetron (GS), effectors regulating gut composition The properties AA GS alleviating resistance, inflammation, oxidative stress verified mice treated high-fat diets. Overall, this study could modulate inflammatory response, capacity, by vertical transmission metabolites. These findings suggest potential prevention treatment MetS life.

Language: Английский

Citations

24
Short-chain fatty acids in nonalcoholic fatty liver disease: New prospects for short-chain fatty acids as therapeutic targets DOI Creative Commons
Xinyu Li, Maozhang He,

Xinrui Yi

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(5), P. e26991 - e26991

Published: Feb. 27, 2024

Nonalcoholic fatty liver disease (NAFLD) is a stress-induced injury related to heredity, environmental exposure and the gut microbiome metabolism. Short-chain acids (SCFAs), metabolites of microbiota (GM), participate in regulation hepatic steatosis inflammation through gut-liver axis, which play an important role alleviation NAFLD. However, little progress has been made systematically elucidating mechanism how SCFAs improve NAFLD, especially epigenetic mechanisms potential therapeutic application as clinical treatment for Herein, we adopted PubMed Medline search relevant keywords such 'SCFAs', 'NAFLD', 'gut microbiota', 'Epigenetic', 'diet', 'prebiotic effect' review latest research on NAFLD up November 2023. In this review, firstly, specifically discussed production function SCFAs, well their crosstalk coordination axis. Secondly, provided updated summary intensive discussion affect alleviate from perspective genetic epigenetic. Thirdly, paid attention pharmacological physiological characteristics proposed promising future direction adopt alone or combination with prebiotics drugs prevent treat Together, aimed elucidate provide new insights prospects target

Language: Английский

Citations

15
Chain conformation, mucoadhesive properties of fucoidan in the gastrointestinal tract and its effects on the gut microbiota DOI
Aijun Chen,

Yatong Liu,

Tao Zhang

et al.

Carbohydrate Polymers, Journal Year: 2022, Volume and Issue: 304, P. 120460 - 120460

Published: Dec. 16, 2022

Language: Английский

Citations

29
Bacteroides ovatus alleviates high-fat and high-cholesterol -induced nonalcoholic fatty liver disease via gut-liver axis DOI Open Access

Changrui Sun,

Xuan Xiong,

Maoyu Liu

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 178, P. 117156 - 117156

Published: July 19, 2024

Gut microbiota acts as a critical regulator in the development of nonalcoholic fatty liver disease (NAFLD), making probiotics promise therapeutic strategy. Studies are needed to identify beneficial Bacteroides strains against NAFLD. ovatus (B. ovatus) may also exhibit therapy effect on The aim this work was evaluate B. NAFLD and examine mechanism. C57BL/6 J male mice were randomly divided into three groups: control group (NCD) that received standard diet, model (M) with high-fat high-cholesterol (HFHC) M_Bo fed HFFC supplemented ovatus. Treatment could reduce body weight, prevent hepatic steatohepatitis injury. Mechanistically, induced changes gut microbial diversity composition, characterized by decreased Firmicutes/Bacteroidetes (F/B) ratio mice, lower abundance Proteobacteria, Verrucomicrobiota at phylum level Ruminococcus_torques_group, Ruminococcus_gauvreauii_group, Erysipelatoclostridium genus level, simultaneously remarkablely higher fecal Lachnospiraceae_NK4A136_group, norank_f__Oscillospiraceae, Colidextribacter. Compared M group, treated showed an markedly altered short chain acids (SCFAs), decline serum levels lipopolysaccharide (LPS), CD163, IL-1β, TNF-α, reduced macrophages livers. Additionally, treatment caused downregulation genes involved denovo lipogenesis (such Srebfl, Acaca, Scd1, Fasn), which accompanied upregulation related acid oxidation Ppara). In conclusion, study provides evidence ameliorate modulating gut-liver axis.

Language: Английский

Citations

8
Chaihu Guizhi Ganjiang Decoction attenuates nonalcoholic steatohepatitis by enhancing intestinal barrier integrity and ameliorating PPARα mediated lipotoxicity DOI
Hao Wu,

Tianyu Lou,

Mingxia Pan

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 326, P. 117841 - 117841

Published: Feb. 3, 2024

Language: Английский

Citations

5
Pea Albumin Extracted from Pea (Pisum sativum L.) Seeds Ameliorates High-Fat-Diet-Induced Non-Alcoholic Fatty Liver Disease by Regulating Lipogenesis and Lipolysis Pathways DOI Open Access
Shucheng Zhang,

Zhengwu Cui,

Hao Zhang

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(14), P. 2232 - 2232

Published: July 11, 2024

Non-alcoholic fatty liver disease (NAFLD) is now recognized as the most prevalent globally. Pea albumin (PA) has demonstrated positive impacts on reducing obesity and improving glucose metabolism. In this research, a mouse model of NAFLD induced by high-fat diet (HFD) was employed to examine impact PA explore its potential mechanisms. The findings revealed that mice subjected HFD developed pronounced alterations. intervention with significantly lowered serum TC 26.81%, TG 43.55%, LDL-C 57.79%. It also elevated HDL-C levels 1.2 fold reduced ALT 37.94% AST 31.21% in fed HFD. These changes contributed reduction hepatic steatosis lipid accumulation. Additionally, improved insulin resistance inhibited oxidative stress inflammatory responses. Mechanistic studies alleviated accumulation HFD-induced activating phosphorylation AMPKα ACC, inhibiting expression SREBF1 FASN reduce lipogenesis, increasing ATGL, PPARα, PPARγ promote lipolysis acid oxidation. results indicate could serve dietary supplement for alleviating NAFLD, offering theoretical foundation rational intake intervention.

Language: Английский

Citations

5
The protective effect of small black soybean (Vigna Mungo L.) polysaccharide on acetic acid-induced gastric ulcer in SD rats and its impact on gut microbiota and metabolites DOI

Zuanyuan Huang,

Mengwei Hu,

Xiuyi Peng

et al.

Food Bioscience, Journal Year: 2023, Volume and Issue: 56, P. 103187 - 103187

Published: Sept. 21, 2023

Language: Английский

Citations

12
Epigallocatechin gallate alleviates osteoporosis by regulating the gut microbiota and serum metabolites in rats DOI
Xuebing Han,

Yifeng Fu,

Keyu Wang

et al.

Food & Function, Journal Year: 2023, Volume and Issue: 14(23), P. 10564 - 10580

Published: Jan. 1, 2023

Osteoporosis, one of the serious public health problems worldwide, can lead to degeneration bone structure and increased risk fractures. Epigallocatechin gallate (EGCG) is a natural product with potential efficacy in inhibiting loss. However, specific mechanism remains unclear. This study first investigated role EGCG preventing dexamethasone (DEX)-induced osteoporosis by regulating intestinal microbiota serum metabolites. We detected density, microstructure, changes microorganisms According our results, inhibited decline protected microbial diversity, promoted abundance beneficial bacteria such as Prevotellaceae Ruminococcus, pathogenic Peptostreptococcaceae. There were also significant metabolites among different treatments. Differential mainly involved sphingolipid metabolism glycerophospholipid pathways, especially ceramide (d18:0/16:0(2OH)), phosphatidylserine (P-20:0/20:4(5Z,8Z,11Z,14Z)), (18:2(9Z,12Z)/12:0), phosphatidylethanolamine (O-16:0/0:00), which after treatment. Notably, most above positively correlated mineral BV/TV Tb·Th, negatively Tb·Sp. In summary, prevent damage, promote production metabolites, enhance immune function. provides basis reference for prevention treatment osteoporosis, well application maintaining body health.

Language: Английский

Citations

11
Silymarin decreases liver stiffness associated with gut microbiota in patients with metabolic dysfunction-associated steatotic liver disease: a randomized, double-blind, placebo-controlled trial DOI Creative Commons

Yufeng Jin,

Xin Wang, Ke Chen

et al.

Lipids in Health and Disease, Journal Year: 2024, Volume and Issue: 23(1)

Published: Aug. 3, 2024

Despite centuries of traditional use silymarin for hepatoprotection, current randomized controlled trial (RCT) studies on the effectiveness in managing metabolic dysfunction-associated steatotic liver disease (MASLD) are limited and inconclusive, particularly when it is administered alone. The low bioavailability highlights possible influence gut microbiota silymarin; however, no human have investigated this aspect. To determine potential efficacy improving MASLD indicators to investigate underlying mechanisms related microbiota. In 24-week randomized, double-blind, placebo-controlled trial, 83 patients with were either placebo (n = 41) or (103.2 mg/d, n 42). At 0, 12, 24 weeks, stiffness hepatic steatosis assessed using FibroScan, blood samples gathered biochemical detection, while faecal collected at 0 weeks 16S rRNA sequencing. Silymarin supplementation significantly reduced (LSM, -0.21 ± 0.17 vs. 0.41 0.17, P 0.015) serum levels γ-glutamyl transpeptidase (GGT, -8.21 3.01 1.23 3.16, 0.042) ApoB (-0.02 0.03 0.07 0.03, 0.023) but had significant effect attenuation parameter (CAP), other (aminotransferases, total bilirubin, glucose lipid parameters, hsCRP, SOD, UA), physical measurements (DBP, SBP, BMI, WHR, BF%, BMR), APRI FIB-4 indices. Gut analysis revealed increased species diversity enrichment Oscillospiraceae group. These findings suggest that could improve patients, possibly by modulating was registered Chinese Clinical Trial Registry (ChiCTR2200059043).

Language: Английский

Citations

4