Transcriptomic and Proteomic Analysis Reveals the Potential Role of RBMS1 in Adipogenesis and Adipocyte Metabolism DOI Open Access
Ghida Dairi, Saeed Al Mahri, Hicham Benabdelkamel

et al.

Published: April 17, 2023

Adipocytes play a critical role in maintaining healthy systemic metabolism by storing and releasing energy the form of fat helping to regulate glucose lipid levels body. Adipogenesis is process through which pre-adipocytes are differentiated into mature adipocytes. It complex involving various transcription factors signaling pathways. Dysregulation adipogenesis has been implicated development obesity metabolic disorders. Therefore, understanding mechanisms that contribute its dysregulation may provide insights prevention treatment these conditions. RNA Binding Motif Single Stranded Interacting Protein 1 (RBMS1) protein binds plays cellular processes such as alternative splicing, mRNA stability, translation. The RBMS1 polymorphism shown be associated with Type 2 diabetes, but adipose not known. We show highly expressed during early phase differentiation murine adipocyte cell line 3T3-L1 significantly upregulated tissue depots adipocytes high-fat-fed mice, implying possible metabolism. Knockdown impacted reduced expression some key adipogenic markers. Transcriptomic proteomic analysis indicated depletion affected several genes involved major processes, including carbohydrate Our findings imply an important offer novel therapeutic opportunity for disorders type diabetes.

Language: Английский

The Metabolic Syndrome, a Human Disease DOI Open Access
M. Alemany

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2251 - 2251

Published: Feb. 13, 2024

This review focuses on the question of metabolic syndrome (MS) being a complex, but essentially monophyletic, galaxy associated diseases/disorders, or just related rather independent pathologies. The human nature MS (its exceptionality in Nature and its close interdependence with action evolution) is presented discussed. text also describes components, special emphasis description their interrelations (including syndromic development recruitment), as well consequences upon energy handling partition. main theories MS’s origin are relation to hepatic steatosis, type 2 diabetes, obesity, encompass most components described so far. differential effects sex biological considered under light social needs evolution, which directly epidemiology, severity, relations senescence. triggering maintenance factors discussed, especial inflammation, complex process affecting different levels organization critical element for development. Inflammation operation connective tissue adipose organ) widely studied acknowledged influence diet. role diet composition, including transcendence anaplerotic Krebs cycle from dietary amino acid supply (and timing), developed context testosterone β-estradiol control insulin-glycaemia core system carbohydrate-triacylglycerol handling. high probability acting unique (essentially monophyletic) presented, together additional perspectives/considerations treatment this ‘very’ disease.

Language: Английский

Citations

30

Inhibition of Pancreatic Lipase by Flavonoid Derivatives: In Vitro and In Silico Investigations DOI Creative Commons
The‐Huan Tran, Tan Thanh, Thi-Thu-Trang Ho

et al.

Advances in Pharmacological and Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 10

Published: Jan. 24, 2024

Obesity, characterized by excessive adipose tissue accumulation, has emerged as a crucial determinant for wide range of chronic medical conditions. The identification effective interventions obesity is utmost importance. Widely researched antiobesity agents focus on pancreatic lipase, significant therapeutic target. This study presented the evaluation ten flavonoid compounds in terms their inhibitory activities against utilizing both vitro and silico approaches. results indicated that all tested demonstrated modest weaker compared to reference compound, orlistat. Among investigated, F01 exhibited highest potency, with an IC50 value 17.68 ± 1.43 µM. enzymatic inhibition kinetic analysis revealed operated through competitive mechanism determined Ki 7.16 μM. suggested moderate binding affinity lipase enzyme. Furthermore, associated id="M2">Vmax was quantified at 0.03272 ΔA·min−1. In studies displayed mode similar orlistat, despite lacking active functional group capable forming covalent bond Ser152 catalytic triad. However, formed hydrogen this amino acid. interacted other residues enzyme’s site, particularly those within lid domain. Based these findings, demonstrates substantial potential candidate further investigations.

Language: Английский

Citations

5

Obesity Aggravates the Clinical Profile of COVID-19 Patients Hospitalized in the North of Mato Grosso, Brazil: A Cohort Study DOI Creative Commons
Karla Kelly Paniago Miranda dos Santos, Mauro André Azevedo Silva Kaiser Cabral, Vinícius Tadeu Ribeiro Mattar

et al.

Obesities, Journal Year: 2025, Volume and Issue: 5(1), P. 4 - 4

Published: Jan. 15, 2025

COVID-19, caused by the SARS-CoV-2 coronavirus, has significantly impacted global health, particularly among patients with obesity. This study evaluates prevalence and effects of overweight obesity on clinical profiles complications COVID-19 admitted to a hospital in Northern Mato Grosso. We conducted retrospective cohort analyzing medical records hospitalized from March 2020 2021. Patients were classified into normal body weight, overweight, groups. Data statistically analyzed using Kruskal–Wallis’s test Dunn’s post-test (continuous variables) or chi-square (χ2) (categorical variables). Among 145 ward records, 24.1% 46.2% 29.7% obese. In intensive care unit, data 243 indicated that 17.3% 37.9% 44.9% obese, highlighting concerning overweight/obesity. Chest computed tomography revealed moderate pulmonary involvement (25–50%) was most frequent group, while severe (>50%) predominant group. The group experienced more complications, including increased use mechanical ventilation. Notably, both settings, mortality rates higher concludes worsen outcomes.

Language: Английский

Citations

0

Distinct phenotypes of patients and healthcare resource utilization after hospitalization for COVID-19: an observational study DOI Creative Commons
Claire Marant Micallef, Manon Belhassen, Florence Ader

et al.

BMC Health Services Research, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 27, 2025

Little is known about postdischarge healthcare resource use (HCU) among patients hospitalized for coronavirus disease 2019 (COVID-19). The objective was to identify distinct profiles of based on cares. This a retrospective cohort study using the French National Health System claims database. We followed up all COVID-19 between 2020/02/01 and 2020/06/30 6 months; discharge date index date. excluded who died during stay or within 30 days after discharge. described patients' HCU over 5 months from day 31 end follow-up, i.e., post-COVID-19 period. sociodemographic clinical characteristics participants 44 selected types HCU, including medical emergency room visits, medications, biological tests, oxygen therapy, rehabilitation, rehospitalization, nurse sick leave. performed Ward's ascendant hierarchical clustering (AHC) analysis groups with similar by cluster. population included 68,822 (median age: 64.8 years, 47% women). Eight clusters were identified, each comprising 1,163 35,501 patients. Four characterized older high proportions comorbidities, i.e. cancer (cluster 3), mental disorders 4), cardiac insufficiency 5) respiratory failure 6). Cluster 8 younger patients, often obese low mortality. Another cluster complex stays 7) last 2) specific contacts therapy. main 1, n = 35,501) overall population. duration complexity also varied across clusters. Based data, AHC identified clinically relevant surviving acute episode hospitalization. illustrate many impacts COVID health status infected may help anticipate future needs care in context.

Language: Английский

Citations

0

Nanotherapeutics in metabolic diseases–Associated infections DOI
V. P. Jaiswal, Shradha Bisht, Swati Prakash

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 617 - 648

Published: Jan. 1, 2025

Language: Английский

Citations

0

A Systematic Review of Metabolic Syndrome: Key Correlated Pathologies and Non-Invasive Diagnostic Approaches DOI Open Access
F. Giangregorio,

Emilio Mosconi,

Maria Grazia Debellis

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(19), P. 5880 - 5880

Published: Oct. 2, 2024

Background and Objectives: Metabolic syndrome (MetS) is a condition marked by complex array of physiological, biochemical, metabolic abnormalities, including central obesity, insulin resistance, high blood pressure, dyslipidemia (characterized elevated triglycerides reduced levels high-density lipoproteins). The pathogenesis develops from the accumulation lipid droplets in hepatocyte (steatosis). This accumulation, genetically predisposed subjects with other external stimuli (intestinal dysbiosis, caloric diet, physical inactivity, stress), activates production pro-inflammatory molecules, alter autophagy, turn on activity hepatic stellate cells (HSCs), provoking low grade chronic inflammation fibrosis. associated significantly increased risk developing type 2 diabetes mellitus (T2D), cardiovascular diseases (CVD), vascular, renal, pneumologic, rheumatological, sexual, cutaneous syndromes overall mortality, rising five- to seven-fold for T2DM, three-fold CVD, one half–fold all-cause mortality. purpose this narrative review examine as “systemic disease” its interaction major internal medicine conditions such diabetes, renal failure, respiratory failure. It essential practitioners approach widespread “holistic” rather than fragmented manner, particularly Western countries. Additionally, it important be aware non-invasive tools available assessing condition. Materials Methods: We conducted an exhaustive search PubMed up July 2024, focusing terms related pathologies (heart, Lung (COPD, asthma, pulmonary hypertension, OSAS) kidney rheumatological (osteoarthritis, rheumatoid arthritis), endocrinological, sexual neoplastic risks. was managed accordance PRISMA statement. Finally, we selected 300 studies (233 papers first strategy 67 second one). Our included that provided insights into techniques evaluating liver fibrosis steatosis. Studies were not humans, published languages English, or did assess changes heart failure excluded. Results: findings revealed clear correlation between all above described, indicating assessments steatosis could potentially serve markers severity progression diseases. Conclusions: multisystem disorder impacts organs beyond disrupts functioning various organs. Notably, linked higher incidence diseases, independent traditional factors. Non-invasive allow clinicians evaluate risk. ability may open new diagnostic, therapeutic, prognostic avenues managing complications, disease, which leading cause death these patients.

Language: Английский

Citations

2

Transcriptomic and Proteomic Analysis Reveals the Potential Role of RBMS1 in Adipogenesis and Adipocyte Metabolism DOI Open Access
Ghida Dairi, Saeed Al Mahri, Hicham Benabdelkamel

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(14), P. 11300 - 11300

Published: July 11, 2023

Adipocytes play a critical role in maintaining healthy systemic metabolism by storing and releasing energy the form of fat helping to regulate glucose lipid levels body. Adipogenesis is process through which pre-adipocytes are differentiated into mature adipocytes. It complex involving various transcription factors signaling pathways. The dysregulation adipogenesis has been implicated development obesity metabolic disorders. Therefore, understanding mechanisms that contribute its may provide insights prevention treatment these conditions. RNA-binding motif single-stranded interacting protein 1 (RBMS1) binds RNA plays cellular processes such as alternative splicing, mRNA stability, translation. RBMS1 polymorphism shown be associated with type 2 diabetes, but adipose not known. We show highly expressed during early phase differentiation murine adipocyte cell line 3T3-L1 significantly upregulated tissue depots adipocytes high-fat-fed mice, implying possible metabolism. Knockdown impacted reduced expression some key adipogenic markers. Transcriptomic proteomic analysis indicated depletion affected several genes involved major processes, including carbohydrate Our findings imply an important offer novel therapeutic opportunity for disorders diabetes.

Language: Английский

Citations

5

Obesity Hypoventilation Syndrome is Associated with Worse In-Hospital Outcomes in Patients with Acute Myocardial Infarction: A Nationwide Study DOI
Abdilahi Mohamoud, N. Ben Abdallah, Mohammed Khalid

et al.

Respiratory Medicine, Journal Year: 2024, Volume and Issue: unknown, P. 107813 - 107813

Published: Sept. 1, 2024

Language: Английский

Citations

0

Transcriptomic and Proteomic Analysis Reveals the Potential Role of RBMS1 in Adipogenesis and Adipocyte Metabolism DOI Open Access
Ghida Dairi, Saeed Al Mahri, Hicham Benabdelkamel

et al.

Published: April 17, 2023

Adipocytes play a critical role in maintaining healthy systemic metabolism by storing and releasing energy the form of fat helping to regulate glucose lipid levels body. Adipogenesis is process through which pre-adipocytes are differentiated into mature adipocytes. It complex involving various transcription factors signaling pathways. Dysregulation adipogenesis has been implicated development obesity metabolic disorders. Therefore, understanding mechanisms that contribute its dysregulation may provide insights prevention treatment these conditions. RNA Binding Motif Single Stranded Interacting Protein 1 (RBMS1) protein binds plays cellular processes such as alternative splicing, mRNA stability, translation. The RBMS1 polymorphism shown be associated with Type 2 diabetes, but adipose not known. We show highly expressed during early phase differentiation murine adipocyte cell line 3T3-L1 significantly upregulated tissue depots adipocytes high-fat-fed mice, implying possible metabolism. Knockdown impacted reduced expression some key adipogenic markers. Transcriptomic proteomic analysis indicated depletion affected several genes involved major processes, including carbohydrate Our findings imply an important offer novel therapeutic opportunity for disorders type diabetes.

Language: Английский

Citations

1