Targeting the Holy Triangle of Quorum Sensing, Biofilm Formation, and Antibiotic Resistance in Pathogenic Bacteria

Microorganisms, Journal Year: 2022, Volume and Issue: 10(6), P. 1239 - 1239

Published: June 16, 2022

Chronic and recurrent bacterial infections are frequently associated with the formation of biofilms on biotic or abiotic materials that composed mono- multi-species cultures bacteria/fungi embedded in an extracellular matrix produced by microorganisms. Biofilm is, among others, regulated quorum sensing (QS) which is interbacterial communication system usually two-component systems (TCSs) secreted autoinducer compounds activate signal transduction pathways through interaction their respective receptors. Embedded biofilms, bacteria protected from environmental stress stimuli, they often show reduced responses to antibiotics, making it difficult eradicate infection. Besides penetration antibiotics intricate structure sessile biofilm-embedded metabolic activity them intrinsically less sensitive antibiotics. Moreover, express elevated levels efflux pumps extrude thereby reducing intracellular levels. Some involved secretion QS biofilm-related materials, besides being important for removing toxic substances bacteria. pump inhibitors (EPIs) have been shown both prevent biofilm sensitize suggesting a relationship between these processes. Additionally, quenchers may affect antibiotic susceptibility. Thus, targeting elements regulate might be promising approach combat antibiotic-resistant infections.

Language: Английский

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Advances in Nanotechnology for Biofilm Inhibition

ACS Omega, Journal Year: 2023, Volume and Issue: 8(24), P. 21391 - 21409

Published: June 7, 2023

Biofilm-associated infections have emerged as a significant public health challenge due to their persistent nature and increased resistance conventional treatment methods. The indiscriminate usage of antibiotics has made us susceptible range multidrug-resistant pathogens. These pathogens show reduced susceptibility intracellular survival. However, current methods for treating biofilms, such smart materials targeted drug delivery systems, not been found effective in preventing biofilm formation. To address this challenge, nanotechnology provided innovative solutions formation by clinically relevant Recent advances nanotechnological strategies, including metallic nanoparticles, functionalized dendrimers, polymeric cyclodextrin-based delivery, solid lipid polymer conjugates, liposomes, may provide valuable technological against infectious diseases. Therefore, it is imperative conduct comprehensive review summarize the recent advancements limitations advanced nanotechnologies. present Review encompasses summary agents, mechanisms that lead formation, impact on human health. In nutshell, offers survey managing infections. A detailed presentation how these strategies improve control prevent key objective mechanisms, applications, prospects nanotechnologies better understanding

Language: Английский

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Revisiting ESKAPE Pathogens: virulence, resistance, and combating strategies focusing on quorum sensing

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: June 29, 2023

The human–bacterial association is long-known and well-established in terms of both augmentations human health attenuation. However, the growing incidents nosocomial infections caused by ESKAPE pathogens ( Enterococcus faecium , Staphylococcus aureus Klebsiella pneumoniae Acinetobacter baumannii Pseudomonas aeruginosa Enterobacter sp.) call for a much deeper understanding these organisms. Adopting holistic approach that includes science infection recent advancements preventing treating imperative designing novel intervention strategies against pathogens. In this regard, review captures ingenious commissioned master players, which are teamed up defenses team, equally, if not more, versatile potent through an analogy. We have taken basketball match as our analogy, dividing bacterial species into two teams playing with ball health. Through we make concept infectious biology more accessible.

Language: Английский

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Wound healing strategies based on nanoparticles incorporated in hydrogel wound patches

RSC Advances, Journal Year: 2023, Volume and Issue: 13(31), P. 21345 - 21364

Published: Jan. 1, 2023

Nanoparticle-modified hydrogels exhibit substantial promise in the realm of wound healing, with their ability to expedite tissue regeneration, mitigate infection risks, and facilitate enhanced therapeutic outcomes.

Language: Английский

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The essential oil of Melaleuca alternifolia incorporated into hydrogel induces antimicrobial and anti-inflammatory effects on infected wounds by Staphylococcus aureus

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 173, P. 116389 - 116389

Published: March 11, 2024

Staphylococcus aureus is one of the most common bacterial isolates found in wounds. Thus, innovative dressings, such as hydrogels, are interesting vehicles for incorporating bioactive compounds like those from Melaleuca alternifolia essential oil (MaEO). In this study, we evaluated antimicrobial and anti-inflammatory potential MaEO incorporated into an alginate chitosan hydrogel treating wounds infected by S. aureus. The with 1% (HMa 1%) was homogeneous a bright pale-yellow color characteristic smell Melaleuca. incorporation does not affect stability hydrogel, which stable up to 90 days storage. Scanning electron microscopy analysis revealed that hydrogels showed irregular surfaces interconnected porous structures accumulations crystals distributed throughout formulation. HMa has high moisture content (95.1%) can absorb simulated wound fluid. Regarding effects, reduced growth ATCC 6538 both vitro conditions ex vivo model using porcine skin. addition, dairy topical treatment murine skin lesions induced significant reduction area, inflammation score, load, well tissue re-epithelialization modulation inflammatory mediators. Therefore, excellent be used pharmacotherapy

Language: Английский

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First report on comprehensive genomic analysis of a multidrug-resistant Enterobacter asburiae isolated from diabetic foot infection from Bangladesh

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 2, 2025

Enterobacter asburiae (E. asburiae) is a gram-negative rod-shaped bacterium which has emerging significance as an opportunistic pathogen having high virulence pattern and drug resistant properties. In this study, we present the detailed analysis of whole genome sequence multidrug-resistant (MDR) E. strain BDW1M3 from Bangladesh. The isolate was collected infected foot wound diabetic ulcer patient. Through sophisticated genomic techniques encompassing sequencing in-depth bioinformatic analyses, research unveils profound understanding isolate's antimicrobial resistance patterns, determinants, biosynthetic gene clusters, metabolic pathways pathogenic potential. displayed to Ampicillin, Fosfomycin, Cefoxitin, Tigecycline, Meropenem, Linezolid, Vancomycin antibiotics demonstrated capacity for biofilm formation. Several genes such blaACT−2,fosA2, baeR, qnrE2, vanA numbers including ybaJ, csrA, barA, uvrY, pgaD, hlyD, hlyC, terC, purD were detected. Metal investigation revealed presence cusCFBA operon system, many other zntA, zitB, czrB. Prophage region Myoviridae Comparative genomics with 47 (n = 47) shed light on genetic diversity strains diverse sources countries, notable observation that both human non-human origins exhibited significant pathogenicity potential, phylogenomic relations hinting at potential cross-species transmission. Pangenome indicated toward expanding pangenome asburiae. Further comprehensive studies are required investigate prevalence in Bangladesh emphasize towards unraveling bacterium's inherent intricate molecular mechanisms underlie its traits

Language: Английский

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Synthesis of tetra-substituted thiophene derivatives as potential Hits combating antibiotic resistant bacteria ESKAPE

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 155, P. 108101 - 108101

Published: Jan. 8, 2025

Language: Английский

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NIAID Workshop Report: Systematic Approaches for ESKAPE Bacteria Antigen Discovery

Vaccines, Journal Year: 2025, Volume and Issue: 13(1), P. 87 - 87

Published: Jan. 18, 2025

On 14–15 November 2023, the National Institute of Allergy and Infectious Diseases (NIAID) organized a workshop entitled “Systematic Approaches for ESKAPE Bacteria Antigen Discovery”. The goal was to engage scientists from diverse relevant backgrounds explore novel technologies that can be harnessed identify address current roadblocks impeding advances in antigen vaccine discoveries pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter species). consisted four sessions addressed infections, discovery efforts, new including systems immunology vaccinology approaches. Each session followed by panel discussion. In total, there were over 260 in-person virtual attendees, with high levels engagement. This report provides summary event highlights challenges opportunities field discovery.

Language: Английский

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The Future of Functional Clothing for an Improved Skin and Textile Microbiome Relationship

Microorganisms, Journal Year: 2021, Volume and Issue: 9(6), P. 1192 - 1192

Published: May 31, 2021

The skin microbiome has become a hot field of research in the last few years. emergence next-generation sequencing given unprecedented insights into impact and involvement microbiota conditions. More more cosmetics contain probiotics or bacteria as an active ingredient, with without scientific data. This is also acknowledged by textile industry. There been holistic approach on how interacts they influence pH, moisture content odour generation. To date, most ingredients have broad-spectrum antibacterial action. manuscript covers current industry developments textiles. It explores nature antimicrobial finishing textiles which can disrupt microbiome, benefits natural friendly therapies to combat conditions, malodour infection.

Language: Английский

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Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: May 23, 2023

Abstract The increasing incidence of infected skin wounds poses a major challenge in clinical practice, especially when conventional antibiotic therapy fails. In this context, bacteriophages emerged as promising alternatives for the treatment antibiotic-resistant bacteria. However, implementation remains hampered by lack efficient delivery approaches to wound tissue. study, bacteriophage-loaded electrospun fiber mats were successfully developed next-generation dressings wounds. We employed coaxial electrospinning approach, creating fibers with protective polymer shell, enveloping core while maintaining their antimicrobial activity. novel exhibited reproducible diameter range and morphology, mechanical properties ideal application onto Further, immediate release kinetics phages confirmed well biocompatibility human cells. Antimicrobial activity was demonstrated against Staphylococcus aureus Pseudomonas aeruginosa core/shell formulation maintained bacteriophage 4 weeks stored at − 20 °C. Based on these characteristics, our approach holds great potential platform technology encapsulation bioactive enable translation phage into application.

Language: Английский

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