Adjustments of the Phytochemical Profile of Broccoli to Low and High Growing Temperatures: Implications for the Bioactivity of Its Extracts

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3677 - 3677

Published: March 26, 2024

Climate change causes shifts in temperature patterns, and plants adapt their chemical content order to survive. We compared the effect of low (LT) high (HT) growing temperatures on phytochemical broccoli (Brassica oleracea L. convar. botrytis (L.) Alef. var. cymosa Duch.) microgreens bioactivity extracts. Using different spectrophotometric, LC-MS/MS, GC-MS, statistical methods, we found that LT increased total phenolics tannins broccoli. The glucosinolates were also by LT; however, they decreased HT. Soluble sugars, known osmoprotectants, both types stress, considerably more HT than LT, suggesting a intense osmotic imbalance. Both detrimental for chlorophyll, with being impactful LT. hormone indole-3-acetic acid, implying an important role broccoli's defense. Ferulic sinapic acid showed trade-off scheme: ferulic while acid. stresses potential act against H2O2 damage mouse embryonal fibroblasts (MEF), human keratinocytes, liver cancer cells. Among tested cell treated H2O2, most significant reduction ROS (36.61%) was recorded MEF cells RT extracts inhibit α-amylase following stresses; inhibition pancreatic lipase only. From perspective nutritional value, based obtained results, conclude conditions result nutritious

Language: Английский

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Natural Inhibitors of Mammalian α-Amylases as Promising Drugs for the Treatment of Metabolic Diseases

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(22), P. 16514 - 16514

Published: Nov. 20, 2023

α-Amylase is a generally acknowledged molecular target of distinct class antidiabetic drugs named α-glucosidase inhibitors. This medications scarce and rather underutilized, treatment with current commercial accompanied by unpleasant adverse effects. However, mammalian α-amylase inhibitors are abundant in nature form an extensive pool high-affinity ligands that available for drug discovery. Individual compounds natural extracts preparations promising therapeutic agents conditions associated impaired starch metabolism, e.g., diabetes mellitus, obesity, other metabolic disorders. review focuses on the structural diversity action mechanisms active products inhibitory activity toward α-amylases, emphasizes proteinaceous as more effective significant potential clinical use.

Language: Английский

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Identification of dual-target isoxazolidine-isatin hybrids with antidiabetic potential: Design, synthesis, in vitro and multiscale molecular modeling approaches

Heliyon, Journal Year: 2024, Volume and Issue: 10(4), P. e25911 - e25911

Published: Feb. 1, 2024

In the development of novel antidiabetic agents, a series isoxazolidine-isatin hybrids were designed, synthesized, and evaluated as dual α-amylase α-glucosidase inhibitors. The precise structures synthesized scaffolds characterized using different spectroscopic techniques elemental analysis. obtained results compared to those reference drug, acarbose (IC

Language: Английский

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Lycopene enriched extra virgin olive oil: Biological activities and assessment of security profile on cells

Food Bioscience, Journal Year: 2024, Volume and Issue: 60, P. 104466 - 104466

Published: June 1, 2024

The production of tomato products is massive in Italy resulting a huge amount waste as seeds, peels and stems. aim our work to re-utilize these matrices through the extraction carotenoids, especially lycopene obtain enriched extra virgin olive oil (EVOO). has been obtained by ultrasound-assisted (UAE) technique using directly solvent, due its lipophilic nature. quantification done HPLC-DAD, good concentration per gram (0.9 mg/g oil). Then EVOO was evaluated vitro determine antioxidant enzyme inhibitory activity comparison with itself. These data were associated cytotoxicity assays vivo histological bioassays, aiming highlight safe healthy profile on human normal cells.

Language: Английский

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Evaluation of the biological efficiency of Terminalia chebula fruit extract against neurochemical changes induced in brain of diabetic rats: an epigenetic study

Inflammopharmacology, Journal Year: 2024, Volume and Issue: 32(2), P. 1439 - 1460

Published: Feb. 8, 2024

Abstract Diabetes mellitus (DM) is a chronic and progressive metabolic disorder that can stimulate neuroinflammation increase oxidative stress in the brain. Therefore, present study was aimed to assess efficacy of ethanolic Terminalia chebula extract against neurochemical histopathological changes induced brains diabetic rats. The clarified reduction rats by significant ( P ≤ 0.05) levels antioxidants with decreasing peroxidation products via T. at both doses (400 600 mg/kg). Moreover, improved brain integrity lowering interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), β-amyloid (Aβ) content, monocyte chemoattractant protein-1 (MCP-1) acetylcholine esterase (ACHE) significantly dose dependent manner compared Severe nuclear pyknosis degeneration were noticed neurons cerebral cortex, hippocampus striatum severity these alterations decreased mg/kg other treated groups. different electrophoretic protein isoenzyme assays revealed lowest similarity index (SI%) values exist control group. quantity most native proteins types increased rats, variations completely diminished extract. concluded ameliorated biochemical, abnormalities when administered mg/kg.

Language: Английский

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Synthesis, antidiabetic activity and in silico studies of benzo[b]thiophene based small molecule α-amylase inhibitors

Journal of Molecular Structure, Journal Year: 2024, Volume and Issue: 1312, P. 138570 - 138570

Published: May 8, 2024

Language: Английский

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Semi-synthesis, α-amylase inhibition, and kinetic and molecular docking studies of arylidene-based sesquiterpene coumarins isolated from Ferula tunetana Pomel ex Batt

RSC Advances, Journal Year: 2024, Volume and Issue: 14(7), P. 4654 - 4665

Published: Jan. 1, 2024

Despite all the significant progress made to enhance efficacy of existing bank drugs used manage and cure type II diabetes mellitus, there is still a need explore novel, effective bioactive compounds with fewer side effects.

Language: Английский

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Assessment of Extraction Methods Effects on the Biological Activities (Antioxidant and Antiamylase) and Chemistry (Total Phenolics and Flavonoids) of Guazuma ulmifolia Leaves

Jordan Journal of Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 17(1), P. 151 - 162

Published: March 19, 2024

The antioxidant activity was tested using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method. Antiamylase evaluated through a colorimetric assay that employs 3,5-dinitro salicylic acid (DNSA) as substrate. Total phenolics and flavonoids content were quantified by highest yield from extraction of G. ulmifolia leaves obtained water extract (9.64%). infusion showed most robust antiamylase activities (IC50 = 6.853 ± 0.504 µg/mL 261.03 6.83 µg/mL, respectively). total found in ethanolic extract, with 69.848 1.871 mg GAE/g 118.854 1.001 QE/g respectively. significantly influenced activity, but not activity. In conclusion, infusions best method for obtaining high even though they did content. Further research is needed to identify compound leaf contribute activities.

Language: Английский

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Novel Properties of Old Propranolol─Assessment of Antiglycation Activity through In Vitro and In Silico Approaches

ACS Omega, Journal Year: 2024, Volume and Issue: 9(25), P. 27559 - 27577

Published: June 11, 2024

Hypertension has earned the "silent killer" nickname since it may lead to a number of comorbidities, including diabetes and cardiovascular diseases. Oxidative stress protein glycation play vital roles in pathogenesis hypertension. Several studies have shown that they profoundly account for vascular dysfunction, endothelial damage, disruption blood pressure regulatory mechanisms. Of particular note are advanced end products (AGEs). AGEs alter tissues' functional mechanical properties by binding receptors (RAGE), stimulating inflammation free radical-mediated pathways. Propranolol, nonselective beta-adrenergic receptor antagonist, is one most commonly used drugs treat hypertension Our study first analyze propranolol's effects on glycoxidation through vitro silico approaches. Bovine serum albumin (BSA) was utilized evaluate inhibition propranolol. Propranolol (1 mM) BSA (0.09 were incubated with different glycating (0.5 M glucose, fructose, galactose 6 days 2.5 mM glyoxal methylglyoxal 12 h) or oxidizing agents (chloramine T 1 h). Biomarkers (Amadori (APs), β-amyloid (βA), (AGEs)), (dityrosine (DT), kynurenine (KYN), N-formylkynurenine (NFK)), oxidation (protein carbonyls (PCs), (AOPPs)) measured means colorimetric fluorimetric methods. The scavenging reactive oxygen species (hydrogen peroxide, hydroxyl radical, nitric oxide) antioxidant capacity (2,2-diphenyl-1-picrylhydrazyl radical ferrous ion chelating (FIC) assays)) propranolol also evaluated. Additionally, docking performed showcase interaction BSA, glycosides, AGE/RAGE pathway proteins. (↓APs, ↓βA, ↓AGEs), (↓DT, ↓KYN, ↓NFK), (↓PCs, ↓AOPPs) prominently decreased samples both glycating/oxidizing factors antiglycoxidant similar those aminoguanidine, known inhibitor, captopril, which an established antioxidant. showed potent activity FIC H2O2 assays, comparable aminoguanidine captopril. In analysis indicated antiglycative during its glycosidases, results confirm decrease vitro. Additional human animal models vivo verification antiglycation activity, as this discovery might hold key prevention diabetic complications among cardiology-burdened patients.

Language: Английский

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Potential nanomedicinal applications and physicochemical nature of Hyphaene thebaica‐reduced nano‐samaria

Microscopy Research and Technique, Journal Year: 2024, Volume and Issue: 87(12), P. 2829 - 2841

Published: July 15, 2024

Herein we described the biofabrication of samarium oxide nanoparticles (HT-Sm

Language: Английский

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