International Journal of Molecular Sciences,
Journal Year:
2022,
Volume and Issue:
24(1), P. 228 - 228
Published: Dec. 23, 2022
In
keeping
with
the
extraordinary
interest
and
advancement
of
extracellular
vesicles
(EVs)
in
pathogenesis
diagnosis
fields,
we
herein
present
an
update
to
knowledge
about
their
role
cystic
fibrosis
(CF)
chronic
obstructive
pulmonary
disease
(COPD).
Although
CF
COPD
stem
from
a
different
origin,
one
genetic
other
acquired,
they
share
similar
pathophysiology,
being
transmembrane
conductance
regulator
(CFTR)
protein
implied
both
disorders.
Various
subsets
EVs,
comprised
mainly
microvesicles
(MVs)
exosomes
(EXOs),
are
secreted
by
various
cell
types
that
either
resident
or
attracted
airways
during
onset
progression
lung
disease,
representing
vehicle
for
metabolites,
proteins
RNAs
(especially
microRNAs),
turn
lead
events
as
such
neutrophil
influx,
overwhelming
proteases
(elastase,
metalloproteases),
oxidative
stress,
myofibroblast
activation
collagen
deposition.
Eventually,
all
these
pathomechanisms
inflammation,
mucus
overproduction,
remodeling
airways,
fibrosis,
thus
operating
complex
interplay
among
cells
tissues.
The
detection
MVs
EXOs
blood
biological
fluids
coming
(bronchoalveolar
lavage
fluid
sputum)
allows
consideration
EVs
cargoes
promising
biomarkers
COPD,
although
clinical
expectations
have
yet
be
fulfilled.
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(30)
Published: June 14, 2024
Abstract
In
recent
decades,
research
on
Extracellular
Vesicles
(EVs)
has
gained
prominence
in
the
life
sciences
due
to
their
critical
roles
both
health
and
disease
states,
offering
promising
applications
diagnosis,
drug
delivery,
therapy.
However,
inherent
heterogeneity
complex
origins
pose
significant
challenges
preparation,
analysis,
subsequent
clinical
application.
This
review
is
structured
provide
an
overview
of
biogenesis,
composition,
various
sources
EVs,
thereby
laying
groundwork
for
a
detailed
discussion
contemporary
techniques
preparation
analysis.
Particular
focus
given
state‐of‐the‐art
technologies
that
employ
microfluidic
non‐microfluidic
platforms
EV
processing.
Furthermore,
this
discourse
extends
into
innovative
approaches
incorporate
artificial
intelligence
cutting‐edge
electrochemical
sensors,
with
particular
emphasis
single
proposes
current
outlines
prospective
avenues
future
research.
The
objective
motivate
researchers
innovate
expand
methods
analysis
fully
unlocking
biomedical
potential.
Cell Communication and Signaling,
Journal Year:
2025,
Volume and Issue:
23(1)
Published: Feb. 11, 2025
Extracellular
vesicles
(EVs)
are
relevant
elements
for
cell-to-cell
communication
and
considered
crucial
in
host-pathogen
interactions
by
transferring
molecules
between
the
pathogen
host
during
infections.
These
structures
participate
various
physiological
pathological
processes
promising
candidates
as
disease
markers,
therapeutic
reagents,
drug
carriers.
Both
H.
pylori
epithelial
cells
infected
secrete
EVs,
which
contribute
to
inflammation
development
of
phenotypes.
However,
many
aspects
cellular
molecular
biology
EV
functions
remain
incompletely
understood
due
methodological
challenges
studying
these
small
structures.
This
review
also
highlights
roles
EVs
derived
from
pylori-infected
pathogenesis
gastric
extragastric
diseases.
Understanding
specific
infections,
whether
advantageous
or
pathogen,
may
help
new
approaches
prevent
disease.
Biomarker Research,
Journal Year:
2023,
Volume and Issue:
11(1)
Published: Nov. 17, 2023
Abstract
While
the
field
of
precision
oncology
is
rapidly
expanding
and
more
targeted
options
are
revolutionizing
cancer
treatment
paradigms,
therapeutic
resistance
particularly
to
immunotherapy
remains
a
pressing
challenge.
This
can
be
largely
attributed
dynamic
tumor-stroma
interactions
that
continuously
alter
microenvironment.
date
most
advancements
have
been
made
through
examining
clinical
utility
tissue-based
biomarkers,
their
invasive
nature
lack
holistic
representation
evolving
disease
in
real-time
manner
could
result
suboptimal
decisions.
Thus,
using
minimally-invasive
approaches
identify
biomarkers
predict
monitor
response
as
well
alert
emergence
recurrences
critical
need.
Currently,
research
efforts
shifting
towards
developing
liquid
biopsy-based
obtained
from
patients
over
course
disease.
Liquid
biopsy
represents
unique
opportunity
intercellular
communication
within
tumor
microenvironment
which
occur
exchange
extracellular
vesicles
(EVs).
EVs
lipid
bilayer
membrane
nanoscale
transfer
plethora
biomolecules
mediate
crosstalk,
shape
microenvironment,
modify
drug
response.
The
capture
innovative
approaches,
such
microfluidics,
magnetic
beads,
aptamers,
allow
analysis
via
high
throughput
multi-omics
techniques
facilitate
use
for
biomarker
discovery.
Artificial
intelligence,
machine
deep
learning
algorithms,
advancing
analyses
uncover
candidate
predictive
signatures
key
translation
into
trials.
With
increasing
recognition
role
mediating
immune
evasion
valuable
source,
these
snapshots
cellular
promising
become
an
important
tool
spur
strategies
block
immunotherapy.
In
this
review,
we
discuss
emerging
describing
current
advances
isolation
function
mediators
We
also
highlight
recent
lung
melanoma
studies
point
application
potential
immuno-oncology.
ACS Applied Nano Materials,
Journal Year:
2024,
Volume and Issue:
7(8), P. 8489 - 8506
Published: April 15, 2024
Cancer
is
the
most
challenging
health
complication
worldwide.
Scientific
society
has
tried
to
decode
its
complications
and
developing
a
smart
solution
for
mankind.
Extracellular
vesicles
(EVs)
are
an
emerging
frontier
in
cancer
research.
EVs
provide
fresh
insight
into
understanding
of
released
from
all
active
cells.
classified
some
major
classes,
such
as
apoptotic
bodies,
microvesicles,
exosomes.
Exosomes
among
exciting
tools
current
research
they
work
cellular
messengers
(which
reflects
cell's
healthy
or
pathological
conditional
status).
In
tumor
microenvironment,
regulate
several
complicated
events
uncontrolled
cell
growth,
immune
suppression,
angiogenesis,
extracellular
matrix
remodeling,
metastasis,
organ-specific
drug
therapeutic
resistance,
stem
development.
also
source
promising
biomarkers
involved
both
diagnosis
prognosis
performs
role.
Exosomes-based
nanomedicine
which
application
nanosize
theranostics
sensor
orientation,
supports
overcoming
management
limitations,
early
therapeutics
specific,
effective,
affordable.
this
review,
we
have
highlighted
integration
exosomes
nanotechnology
cutting-edge
approach
precision
oncology.
Cancer Cell International,
Journal Year:
2024,
Volume and Issue:
24(1)
Published: Aug. 7, 2024
Exosomes
are
extracellular
vesicles
well
known
for
facilitating
cell-to-cell
communication
by
distributing
essential
macromolecules
like
proteins,
DNA,
mRNA,
lipids,
and
miRNA.
These
abundant
in
fluids
distributed
throughout
the
body,
including
urine,
blood,
saliva,
even
bile.
They
important
diagnostic
tools
breast,
lung,
gastrointestinal
cancers,
etc.
However,
their
application
as
cancer
biomarkers
has
not
yet
been
implemented
most
parts
of
world.
In
this
review,
we
discuss
how
OMICs
profiling
exosomes
can
be
practiced
substituting
traditional
imaging
or
biopsy
methods
detection.
Previous
extensive
used
screening
were
expensive,
mostly
invasive,
could
easily
provide
early
detection
various
types
cancer.
Exosomal
utilized
routine
simply
collecting
body
from
individual.
We
anticipate
that
use
will
brought
to
light
success
clinical
trials
investigating
potential
enhance
treatment
upcoming
years.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(8), P. 7208 - 7208
Published: April 13, 2023
Breast
cancer
(BC)
is
the
first
worldwide
most
frequent
in
both
sexes
and
commonly
diagnosed
females.
Although
BC
mortality
has
been
thoroughly
declining
over
past
decades,
there
are
still
considerable
differences
between
women
with
early
when
metastatic
diagnosed.
treatment
choice
widely
dependent
on
precise
histological
molecular
characterization.
However,
recurrence
or
distant
metastasis
occurs
even
recent
efficient
therapies.
Thus,
a
better
understanding
of
different
factors
underlying
tumor
escape
mainly
mandatory.
Among
leading
candidates
continuous
interplay
cells
their
microenvironment,
where
extracellular
vesicles
play
significant
role.
vesicles,
smaller
ones,
also
called
exosomes,
can
carry
biomolecules,
such
as
lipids,
proteins,
nucleic
acids,
generate
signal
transmission
through
an
intercellular
transfer
content.
This
mechanism
allows
to
recruit
modify
adjacent
systemic
microenvironment
support
further
invasion
dissemination.
By
reciprocity,
stromal
use
exosomes
profoundly
cell
behavior.
review
intends
cover
literature
role
vesicle
production
normal
cancerous
breast
tissues.
Specific
attention
paid
for
diagnosis,
follow-up,
prognosis
because
actually
under
spotlight
researchers
high-potential
source
liquid
biopsies.
Extracellular
new
targets
therapy
nanovectors
drive
drug
delivery
summarized.
Biomolecules,
Journal Year:
2023,
Volume and Issue:
13(6), P. 897 - 897
Published: May 27, 2023
The
tumor
microenvironment
(TME)
plays
an
important
role
in
the
development
and
progression
of
hematological
malignancies.
In
recent
years,
studies
have
focused
on
understanding
how
cells
communicate
within
TME.
addition
to
several
factors,
such
as
growth
cytokines,
extracellular
matrix
(ECM)
molecules,
etc.,
a
growing
body
evidence
has
indicated
that
vesicles
(EVs)
play
crucial
communication
TME,
thereby
contributing
pathogenesis
present
review
focuses
EVs
derived
from
interact
with
immune
cells,
stromal
endothelial
ECM
components,
vice
versa,
context
various
recovered
fluids
cancer
patients
often
carry
bioactive
molecules
originating
hence
can
be
considered
new
predictive
biomarkers
for
specific
types
cancer,
also
acting
potential
therapeutic
targets.
Here,
we
discuss
influence
via
tumor-host
crosstalk
their
use
malignancies,
benefiting
Cancers,
Journal Year:
2023,
Volume and Issue:
15(5), P. 1456 - 1456
Published: Feb. 24, 2023
After
a
short
introduction
about
the
history
of
liquid
biopsy,
aimed
to
noninvasively
replace
common
tissue
biopsy
as
help
for
cancer
diagnosis,
this
review
is
focused
on
extracellular
vesicles
(EVs),
main
third
component,
which
now
coming
into
light
biopsy.
Cell-derived
EV
release
recently
discovered
general
cellular
property,
and
EVs
harbor
many
components
reflecting
their
cell
origin.
This
also
case
tumoral
cells,
cargoes
might
therefore
be
“treasure
chest”
biomarkers.
has
been
extensively
explored
decade,
but
EV-DNA
content
escaped
worldwide
query
until
recently.
The
aim
gather
pilot
studies
DNA
circulating
cell-derived
EVs,
following
five
years
tumor
EV-DNA.
recent
preclinical
tEV-derived
gDNA
potential
biomarker
developed
puzzling
controversy
presence
exosomes,
coupled
with
an
increased
unexpected
non
vesicular
complexity
environment.
discussed
in
present
review,
together
challenges
that
need
solved
before
any
efficient
clinical
transfer
quite
promising
diagnosis
biomarker.
