Live‐cell imaging of cell signaling using genetically encoded fluorescent reporters DOI Open Access

Qiang Ni,

Sohum Mehta, Jin Zhang

et al.

FEBS Journal, Journal Year: 2017, Volume and Issue: 285(2), P. 203 - 219

Published: June 14, 2017

Synergistic advances in fluorescent protein engineering and live-cell imaging techniques recent years have fueled the concurrent development application of genetically encoded reporters that are tailored for tracking signaling dynamics living systems over multiple length time scales. These biosensors uniquely suited this challenging task, owing to their specificity, sensitivity, versatility, as well noninvasive nondestructive nature fluorescence power genetic encoding. Over past 10 years, a growing number been developed wide range biological signals cells animals, including second messenger metabolite dynamics, enzyme activation activity, cell cycle progression neuronal activity. Many these gaining use proving be indispensable unraveling complex functions individual molecules native environment, cell, shedding new light on structural molecular underpinnings signaling. In review, we highlight likely help expand improve design valuable tools. We then turn our focus specific examples using an important platform advancing understanding G protein-coupled receptor

Language: Английский

Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications DOI Creative Commons
Rupsa Datta, Tiffany M. Heaster, Joe T. Sharick

et al.

Journal of Biomedical Optics, Journal Year: 2020, Volume and Issue: 25(07), P. 1 - 1

Published: May 13, 2020

Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to distinguish the unique molecular environment of fluorophores. FLIM measures time fluorophore remains in an excited state before emitting photon, and detects variations fluorophores that are not apparent with spectral techniques alone. sensitive multiple biomedical processes including disease progression drug efficacy. Aim: We provide overview principles, instrumentation, analysis while highlighting latest developments biological applications. Approach: This review covers principles theory, advantages over intensity-based fluorescence measurements. Fundamentals instrumentation time- frequency-domains summarized, along recent developments. Image segmentation strategies quantify spatial features cellular heterogeneity reviewed. Finally, representative applications provided high-resolution cell- organelle-level changes, use exogenous endogenous fluorophores, protein-protein interactions Förster resonance energy transfer (FRET). Advantages limitations also discussed. Conclusions: advantageous for probing environments inform on behavior cannot be elucidated intensity measurements Development technologies, analysis, will further advance research clinical assessments.

Language: Английский

Citations

606
FRET as a biomolecular research tool — understanding its potential while avoiding pitfalls DOI
W. Russ Algar, Niko Hildebrandt, Steven S. Vogel

et al.

Nature Methods, Journal Year: 2019, Volume and Issue: 16(9), P. 815 - 829

Published: Aug. 30, 2019

Language: Английский

Citations

483
Photoluminescent Nanoparticles for Chemical and Biological Analysis and Imaging DOI
W. Russ Algar,

Melissa Massey,

Kelly Rees

et al.

Chemical Reviews, Journal Year: 2021, Volume and Issue: 121(15), P. 9243 - 9358

Published: July 20, 2021

Research related to the development and application of luminescent nanoparticles (LNPs) for chemical biological analysis imaging is flourishing. Novel materials new applications continue be reported after two decades research. This review provides a comprehensive heuristic overview this field. It targeted both newcomers experts who are interested in critical assessment LNP materials, their properties, strengths weaknesses, prospective applications. Numerous cataloged by fundamental descriptions identities physical morphology, quantitative photoluminescence (PL) PL mechanisms, surface chemistry. These include various semiconductor quantum dots, carbon nanotubes, graphene derivatives, nanodiamonds, metal nanoclusters, lanthanide-doped upconversion downshifting nanoparticles, triplet–triplet annihilation persistent-luminescence conjugated polymer semiconducting multi-nanoparticle assemblies, doped labeled including but not limited those based on polymers silica. As an exercise these ranked several application-related functional criteria. Additional sections highlight recent examples advances analysis, point-of-care diagnostics, cellular, tissue, vivo theranostics. drawn from literature organized material particular properties that leveraged advantage. Finally, perspective what comes next field offered.

Language: Английский

Citations

248
Fluorescent indicators for simultaneous reporting of all four cell cycle phases DOI
Bryce T. Bajar,

Amy Lam,

Ryan K. Badiee

et al.

Nature Methods, Journal Year: 2016, Volume and Issue: 13(12), P. 993 - 996

Published: Oct. 31, 2016

Language: Английский

Citations

200
Recent advances in neurotechnologies with broad potential for neuroscience research DOI
Abraham Vázquez‐Guardado, Yiyuan Yang, Amay J. Bandodkar

et al.

Nature Neuroscience, Journal Year: 2020, Volume and Issue: 23(12), P. 1522 - 1536

Published: Nov. 16, 2020

Language: Английский

Citations

170
TLR4 signalling via Piezo1 engages and enhances the macrophage mediated host response during bacterial infection DOI Creative Commons
Jing Geng,

Yiran Shi,

Jinjia Zhang

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: June 10, 2021

TLR4 signaling plays key roles in the innate immune response to microbial infection. Innate cells encounter different mechanical cues both health and disease adapt their behaviors. However, impact of sensing signals on signal-mediated remains unclear. Here we show that signalling augments macrophage bactericidal activity through sensor Piezo1. Bacterial infection or LPS stimulation triggers assembly complex Piezo1 remodel F-actin organization augment phagocytosis, mitochondrion-phagosomal ROS production bacterial clearance genetic deficiency results abrogation these responses. Mechanistically, stimulates induce Piezo1-mediated calcium influx consequently activates CaMKII-Mst1/2-Rac axis for pathogen ingestion killing. Inhibition CaMKII knockout either Mst1/2 Rac1 reduced activity, phenocopying deficiency. Thus, conclude drives via providing critical insight understanding mechanophysiology host response.

Language: Английский

Citations

170
Schiff Base Aggregation-Induced Emission Luminogens for Sensing Applications: A Review DOI

Jingfei Wang,

Qingye Meng,

Yongyan Yang

et al.

ACS Sensors, Journal Year: 2022, Volume and Issue: 7(9), P. 2521 - 2536

Published: Sept. 1, 2022

Fluorescence sensing can not only identify a target substrate qualitatively but also achieve the purpose of quantitative detection through change fluorescence signal. It has advantages immense sensitivity, rapid response, and excellent selectivity. The proposed aggregation-induced emission (AIE) concept solves problem traditional fluorescent molecules becoming weak or quenched in high concentration aggregated state conditions. Schiff base probes have simple synthesis, low toxicity, easy design. They are often used for various substances. In this review we cover late developments compounds with AIE characteristics working as sensors.

Language: Английский

Citations

89
Fluorescent proteins and genetically encoded biosensors DOI
Minji Wang,

Yifan Da,

Yang Tian

et al.

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(4), P. 1189 - 1214

Published: Jan. 1, 2023

The genetically encoded fluorescent sensors convert chemical and physical signals into light. They are powerful tools for the visualisation of physiological processes in living cells freely moving animals. protein is reporter module a biosensor. In this study, we first review history full emission spectra on structural basis. Then, discuss design Finally, briefly several major types biosensors that currently widely used based their molecular targets, which may be useful future biosensors.

Language: Английский

Citations

75
FRET Based Biosensor: Principle Applications Recent Advances and Challenges DOI Creative Commons
Awadhesh Kumar Verma, Ashab Noumani, Amit K. Yadav

et al.

Diagnostics, Journal Year: 2023, Volume and Issue: 13(8), P. 1375 - 1375

Published: April 8, 2023

Förster resonance energy transfer (FRET)-based biosensors are being fabricated for specific detection of biomolecules or changes in the microenvironment. FRET is a non-radiative from an excited donor fluorophore molecule to nearby acceptor molecule. In FRET-based biosensor, and molecules typically fluorescent proteins nanomaterials such as quantum dots (QDs) small that engineered be close proximity each other. When biomolecule interest present, it can cause change distance between acceptor, leading efficiency corresponding fluorescence intensity acceptor. This used detect quantify interest. have wide range applications, including fields biochemistry, cell biology, drug discovery. review article provides substantial approach on principle, applications point-of-need diagnosis, wearable, single molecular (smFRET), hard water, ions, pH, tissue-based sensors, immunosensors, aptasensor. Recent advances artificial intelligence (AI) Internet Things (IoT) this type sensor challenges.

Language: Английский

Citations

73
Recent advances in sensing the inter-biomolecular interactions at the nanoscale – A comprehensive review of AFM-based force spectroscopy DOI Creative Commons
Anabel Lostao, Keesiang Lim,

María Carmen Pallarés

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 238, P. 124089 - 124089

Published: March 21, 2023

Biomolecular interactions underpin most processes inside the cell. Hence, a precise and quantitative understanding of molecular association dissociation events is crucial, not only from fundamental perspective, but also for rational design biomolecular platforms state-of-the-art biomedical industrial applications. In this context, atomic force microscopy (AFM) appears as an invaluable experimental technique, allowing measurement mechanical strength complexes to provide characterization their interaction properties single molecule perspective. present review, recent methodological advances in field are presented with special focus on bioconjugation, immobilization AFM tip functionalization, dynamic spectroscopy measurements, recognition imaging theoretical modeling. We expect work significantly aid grasping principles AFM-based (AFM-FS) technique necessary tools acquaint type data that can be achieved experiments. Furthermore, critical assessment done other nanotechnology techniques better visualize future prospects AFM-FS.

Language: Английский

Citations

52