Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 489, P. 137636 - 137636
Published: Feb. 16, 2025
Language: Английский
Small, Journal Year: 2023, Volume and Issue: 20(14)
Published: Nov. 20, 2023
Abstract Nanozymes, as innovative materials, have demonstrated remarkable potential in the field of electrochemical biosensors. This article provides an overview mechanisms and extensive practical applications nanozymes First, definition characteristics are introduced, emphasizing their significant role constructing efficient sensors. Subsequently, several common categories nanozyme materials delved into, including metal‐based, carbon‐based, metal‐organic framework, layered double hydroxide nanostructures, discussing Regarding mechanisms, two key roles particularly focused biosensors: selective enhancement signal amplification, which crucially support sensor performance. In terms applications, widespread use nanozyme‐based biosensors showcased various domains. From detecting biomolecules, pollutants, nucleic acids, proteins, to cells, providing robust means for high‐sensitivity detection. Furthermore, insights into future development is provided, encompassing improvements optimizations design integration, expansion application fields through interdisciplinary collaboration. conclusion, this systematically presents biosensors, offering valuable references prospects research field.
Language: Английский
Citations
54
Biology of the Cell, Journal Year: 2025, Volume and Issue: 117(2)
Published: Feb. 1, 2025
Abstract Background Information The discovery of green fluorescent protein (GFP) and its derivatives has revolutionized cell biology. These proteins (FPs) have enabled the real‐time observation localization dynamics within live cells. Applications FP vary from monitoring gene/protein expression patterns, visualizing protein‐protein interactions, measuring stability, assessing mobility, creating biosensors. utility FPs also extends to biochemical approaches through immunoblotting proteomic analyses, aided by anti‐FP antibodies nanobodies. are notoriously robust with a tightly folded domain that confers strong stability relative resistance degradation denaturation. Methods Results In this study, we report various green, orange, red can be maintained in native, state during entire process sample extraction, incubation buffer, loading, migration on SDS‐PAGE (sodium dodecyl sulfate‐polyacrylamide gel electrophoresis) only minor adaptations traditional protocols. This protocol results ability detect quantify in‐gel fluorescence (IGF) endogenously‐expressed tagged directly after migration, using standard fluorescence‐imaging devices. approach eliminates need for chemiluminescent reagents, as well time‐consuming steps inherent such transfer onto membrane antibody incubations. Conclusions Significance Overall, IGF detection provides clearer data less background interference, sensitivity comparable or better than antibody‐based detection, quantification, broader dynamic range. After imaging, gels still used other applications total staining if needed. It expands possibilities allowing which not available. Our study explores feasibility, limitations, detecting endogenously expressed extracts, providing insights into preparation, imaging conditions, optimizations, potential co‐immunoprecipitation experiments.
Language: Английский
Citations
2
Chemosensors, Journal Year: 2023, Volume and Issue: 11(4), P. 226 - 226
Published: April 6, 2023
Metal ions play a crucial role in many biochemical processes, and when state of scarcity or surplus, they can lead to various diseases. Therefore, the development selective, sensitive, cost-effective, fast-responding sensor detect metal is critical for vitro medical diagnostics. In recent years, fluorescent sensors have been extensively investigated as potent kits effective assessment living systems due their high sensitivity, selectivity, ability perform real-time, non-invasive monitoring, versatility. This review an overview advances detection imaging biosystems from 2018 date. Specifically, we discuss application detecting essential non-essential diagnostics, cell imaging, vivo imaging. Finally, summarize remaining challenges offer future outlook on above topics.
Language: Английский
Citations
34
Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(16), P. 5352 - 5372
Published: Jan. 1, 2023
T cells are an essential component of the immune system and display multiple biological functions. Smart probes range from small fluorophores to nanoconstructs, can target metabolic enzymatic biomarkers as well cell-surface receptors.
Language: Английский
Citations
24
Biotechnology Advances, Journal Year: 2024, Volume and Issue: 72, P. 108339 - 108339
Published: March 18, 2024
Language: Английский
Citations
13
Nature Methods, Journal Year: 2024, Volume and Issue: 21(10), P. 1916 - 1925
Published: Sept. 20, 2024
Abstract Genetically encoded fluorescent calcium indicators allow cellular-resolution recording of physiology. However, bright, genetically targetable that can be multiplexed with existing tools in vivo are needed for simultaneous imaging multiple signals. Here we describe WHaloCaMP, a modular chemigenetic indicator built from bright dye-ligands and protein sensor domains. Fluorescence change WHaloCaMP results reversible quenching the bound dye via strategically placed tryptophan. is compatible rhodamine fluoresce green to near-infrared, including several efficiently label brain animals. When near-infrared dye-ligand, shows 7× increase fluorescence intensity 2.1-ns lifetime upon binding. We use WHaloCaMP1a image Ca 2+ responses flies mice, perform three-color functional hundreds neurons astrocytes zebrafish larvae quantify concentration using microscopy (FLIM).
Language: Английский
Citations
10
Cell Reports Methods, Journal Year: 2025, Volume and Issue: unknown, P. 100987 - 100987
Published: Feb. 1, 2025
Language: Английский
Citations
1
Physical Chemistry Chemical Physics, Journal Year: 2024, Volume and Issue: 26(13), P. 9816 - 9847
Published: Jan. 1, 2024
This review article explores the fascinating realm of fluorescence using organochalcogen molecules, with a particular emphasis on tellurium (Te). The discussion encompasses underlying mechanisms, structural motifs influencing fluorescence, and applications these intriguing phenomena. not only elucidates current state knowledge but also identifies avenues for future research, thereby serving as valuable resource researchers enthusiasts in field chemistry focus Te-based molecules. By highlighting challenges prospects, this sparks conversation transformative potential Te-containing compounds across different fields, ranging from environmental solutions to healthcare materials science applications. aims provide comprehensive understanding distinct behaviors exhibited by compounds, contributing insights evolving landscape chalcogen-based research.
Language: Английский
Citations
8
Molecules, Journal Year: 2024, Volume and Issue: 29(11), P. 2542 - 2542
Published: May 28, 2024
Zinc ions (Zn2+) play a key role in maintaining and regulating protein structures functions. To better understand the intracellular Zn2+ homeostasis signaling role, various fluorescent sensors have been developed that allow monitoring of concentrations bioimaging live cells real time. This review highlights recent development organic probes for detection imaging Zn2+, including design construction probes, response mechanisms, their applications to on-site. Finally, current challenges prospects are discussed.
Language: Английский
Citations
8
Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(19), P. 9446 - 9489
Published: Jan. 1, 2024
Proteins, which are ubiquitous in cells and critical to almost all cellular functions, indispensable for life. Fluorescence imaging of proteins is key understanding their functions within native milieu, as it provides insights into protein localization, dynamics, trafficking living systems. Consequently, the selective labeling target with fluorophores has emerged a highly active research area, encompassing bioorganic chemistry, chemical biology, cell biology. Various methods selectively tissues have been established continually being developed visualize characterize proteins. This review highlights findings reported since 2018, focus on small organic biological applications studying protein-associated events. We also discuss strengths weaknesses each approach utility
Language: Английский
Citations
8