RSM assisted phytochemical profiling of Vitex negundo and molecular docking studies of biological active compounds with target proteins
Abstract
Vitex
negundo (Family-Verbenaceae)
is
a
remarkable
medicinal
plant
with
numerous
therapeutic
applications.
In
this
study,
phytochemical
screening
was
performed
in
negundoleaf
extract,
and
extraction
optimization
by
applying
response
surface
methodology
(RSM).
Methanolic
extract
microwave-assisted
(MAE)
were
found
to
be
appropriate
for
obtaining
the
maximum
yield
of
bioactive
compounds.
single-factor
analysis,
solvent
concentration,
microwave
power,
time
variations
analyzed
calculate
amount
TPC,
TFC,
TAC
extract.
The
quantity
fairly
obtained
from
60%–95%
(v/v)
methanol
540
-900
W
60–90
s
time.
Furthermore,
we
also
employed
(RSM)
optimizing
negundo
leaves
considering
three
key
parameters:
temperature,
duration.
Condition
at
95%
methanol,
720W,
90s
TPC
(227.6
mg
gallic
acid
equivalent
(GAE)/g
sample),
TFC
(978.2
RE/g
(82.13
ascorbic
equivalents/g
sample).
characterization
negundowas
done
FTIR
GC–MS
identify
various
active
groups
types
constituent’s
presence
Subsequently,
compounds
evaluated
their
bioactivity
through
molecular
docking
interactions
target
proteins
in
silico .
antibacterial
activity
optimized
studied
against
two
Gram
+ve
(Bacillus
sphaericus,
Pectobacteriu m)
Gram-ve
(Escherichia
coli,
Pseudomonas
aeruginosa )
bacteria,
inhibitory
effect
observed
all
four
tested
bacteria.
highest
shown
B.
sphaericus
(19±
0.087)
E.
coli
(19±0.064)
followed
Pectobacterium
(18±0.068)
Pseudomonas (13±0.093).
hemolytic
leaf
(at
dose
20µg/ml)
shows
minimum
activity,
i.e.,
1.33±
0.06
%.
It
may
concluded
study
that
potential
safe
source
natural
antioxidants
can
utilized
green
therapeutics.
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: April 4, 2024
Language: Английский