The link between gestational diabetes and cardiovascular diseases: potential role of extracellular vesicles

Cardiovascular Diabetology, Journal Year: 2022, Volume and Issue: 21(1)

Published: Sept. 3, 2022

Abstract Extracellular vesicles are critical mediators of cell communication. They encapsulate a variety molecular cargo such as proteins, lipids, and nucleic acids including miRNAs, lncRNAs, circular RNAs, mRNAs, through transfer these signals can alter the metabolic phenotype in recipient cells. Emerging studies show important role extracellular vesicle signaling development progression cardiovascular diseases associated risk factors type 2 diabetes obesity. Gestational mellitus (GDM) is hyperglycemia that develops during pregnancy increases future developing obesity, impaired glucose metabolism, disease both mother infant. Available evidence shows changes maternal metabolism exposure to hyperglycemic intrauterine environment reprogram fetal genome, leaving imprints define life-long health susceptibility. Understanding contribute increased susceptibility disorders children born GDM mothers for implementation preventive strategies GDM. In this review, we discuss current literature on programming impact (EV) epigenetic disease, determine potential link between EV infants.

Language: Английский

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Extracellular vesicles and their non‐coding RNA cargos: Emerging players in cardiovascular disease

The Journal of Physiology, Journal Year: 2022, Volume and Issue: 601(22), P. 4989 - 5009

Published: Sept. 12, 2022

Extracellular vesicles (EVs), including exosomes, microvesicles and apoptotic bodies, have recently received attention as essential mechanisms for cell-to-cell communication in cardiovascular disease. EVs can be released from different types of cells, endothelial smooth muscle cardiac fibroblasts, platelets, adipocytes, immune cells stem cells. Non-coding (nc)RNAs EV cargos been investigated the system. Up- or downregulated ncRNAs shown to play a crucial role various diseases. Communication via EV-derived occur between same type involved pathophysiology In present review, we highlight important aspects diverse cell-derived their ncRNA disease mediators potential therapeutic targets atherosclerosis, coronary artery disease, ischaemic heart fibrosis. addition, summarize treatment Finally, discuss methods isolation characterization. A better understanding specific regulation (dys)function will importance development diagnostic tools

Language: Английский

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KLF5/LINC00346/miR‑148a‑3p axis regulates inflammation and endothelial cell injury in atherosclerosis

International Journal of Molecular Medicine, Journal Year: 2021, Volume and Issue: 48(2)

Published: June 17, 2021

Atherosclerosis (AS) is the main pathological basis of cardiovascular diseases, which are related to high morbidity and mortality rates. The present study aimed investigate role Krüppel‑like factor 5 (KLF5)/LINC00346/miR‑148a‑3p loop in AS. expression levels KLF5 serum KLF5/LINC00346/miR‑148a‑3p human umbilical vein endothelial cells (HUVECs) were detected by RT‑qPCR analysis. protein KLF5, phosphorylated (p‑)endothelial nitric oxide synthase (eNOS) eNOS HUVECs analyzed western blot Changes TNF‑α, IL‑1β, IL‑6 (NO) determined supernatant through application available commercial kits. binding promoter region LINC00346 was verified chromatin immunoprecipitation (ChIP)‑PCR assay. combinatory interaction between LINC00346, miR‑148a‑3p, miR‑148a‑3p confirmed luciferase reporter results revealed that increased patients with AS also oxidized low‑density lipoprotein (OX‑LDL)‑stimulated HUVECs. transcription promoted LINC00346. interference or inhibited inflammatory factors functional injury OX‑LDL‑stimulated functioned as a sponge miR‑148a‑3p. overexpression targeted expression. On whole, demonstrates induces downregulation inhibits inflammation OX upregulating

Language: Английский

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Clinical Parameters and Epigenetic Biomarkers of Plaque Vulnerability in Patients with Carotid Stenosis

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(9), P. 5149 - 5149

Published: May 5, 2022

Atheromatous disease is the first cause of death and dependency in developed countries carotid artery atherosclerosis one main causes severe ischaemic strokes. Current management strategies are mainly based on degree stenosis patient selection has limited accuracy. This information could be complemented by identification biomarkers plaque vulnerability, which would permit patients at greater lesser risk stroke to distinguished, thus enabling a better for surgical or intensive medical treatment. Although several circulating protein-based with significance both diagnosis its prognosis have been identified, present, none clinically implemented. review focuses especially most relevant clinical parameters take into account routine practice summarises up-to-date data epigenetic vulnerability.

Language: Английский

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Circular RNAs Variously Participate in Coronary Atherogenesis

Current Issues in Molecular Biology, Journal Year: 2023, Volume and Issue: 45(8), P. 6682 - 6700

Published: Aug. 13, 2023

Over the past decade, numerous studies have shown that circular RNAs (circRNAs) play a significant role in coronary artery atherogenesis and other cardiovascular diseases. They belong to class of non-coding arise as result non-canonical splicing premature RNA, which results formation closed single-stranded circRNA molecules lack 5′-end caps 3′-end poly(A) tails. circRNAs broad post-transcriptional regulatory activity. Acting sponge for miRNAs, compete with mRNAs binding acting competing endogenous RNAs. Numerous are involved circRNA–miRNA–mRNA axes associated pathogenesis cardiomyopathy, chronic heart failure, hypertension, atherosclerosis, disease. Recent сirc_0001445, circ_0000345, circ_0093887, сircSmoc1-2, circ_0003423 disease (CAD) an atheroprotective effect, while circ_0002984, circ_0029589, circ_0124644, circ_0091822, circ_0050486 possess proatherogenic effect. With their high resistance endonucleases, promising diagnostic biomarkers therapeutic targets. This review aims provide updated information on involvement atherogenesis-related CAD. We also discuss main modern approaches detecting studying interactions, well prospects using targets treatment

Language: Английский

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Impact of CircRNAs on Ischemic Stroke

Aging and Disease, Journal Year: 2022, Volume and Issue: 13(2), P. 329 - 329

Published: Jan. 1, 2022

Circular RNA (circRNA) is a recently discovered class of endogenous non-coding that generated by cyclization, driven intron pairing, and regulated related regulators. An important biological function CircRNA acting as molecular sponge to significantly alter miRNA levels over short period. Several studies have shown circRNA closely stroke. Therefore, better understanding regulatory mechanism in ischemic brain will help us for the early detection, diagnosis, treatment Here, we summary characteristics, expression circRNA, its effect on outcome after

Language: Английский

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Identification of a Novel Angiogenesis Signalling circSCRG1/miR-1268b/NR4A1 Pathway in Atherosclerosis and the Regulatory Effects of TMP-PF In Vitro

Molecules, Journal Year: 2023, Volume and Issue: 28(3), P. 1271 - 1271

Published: Jan. 28, 2023

Angiogenesis contributes to plaque instability in atherosclerosis and further increases cardio-cerebrovascular risk. Circular RNAs (circRNAs) are promising biomarkers potential therapeutic targets for atherosclerosis. Previous studies have demonstrated that tetramethylpyrazine (TMP) paeoniflorin (PF) combination treatment (TMP-PF) inhibited oxidized low-density lipoprotein (ox-LDL)-induced angiogenesis vitro. However, whether circRNAs regulate TMP-PF can angiogenesis-related target unknown. In this study, human RNA sequencing (RNA-seq) data were analysed identify differentially expressed (DE) obtain angiogenesis-associated circRNA-microRNA (miRNA)-messenger (mRNA) networks. Target circRNA-related mechanisms the regulatory effects of on circRNA signalling studied ox-LDL-induced umbilical vein endothelial cells (HUVECs) by cell proliferation, migration, tube formation, luciferase reporter assays, real-time quantitative polymerase chain reaction (RT-qPCR) Western blotting. A novel (circular stimulator chondrogenesis 1, circSCRG1) was initially identified associated with atherosclerosis, circSCRG1 silencing up-regulated miR-1268b expression, increased nuclear receptor subfamily 4 group member 1 (NR4A1) expression then promoted angiogenesis. (1 μmol/L TMP combined 10 PF) mediated suppress NR4A1 abolished inhibitory angiogenesis, which rescued inhibitor. conclusion, might serve as a new regulating via circSCRG1/miR-1268b/NR4A1 axis could inhibit vitro, indicating pathway TMP-PF, provide pharmaceutical strategy combat atherosclerotic instability.

Language: Английский

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Analysis of expression profiles and bioinformatics suggests that plasma exosomal circular RNAs may be involved in ischemic stroke in the Chinese Han population

Metabolic Brain Disease, Journal Year: 2022, Volume and Issue: 37(3), P. 665 - 676

Published: Jan. 24, 2022

Language: Английский

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The Role of Clonal Hematopoiesis of Indeterminant Potential and DNA (Cytosine-5)-Methyltransferase Dysregulation in Pulmonary Arterial Hypertension and Other Cardiovascular Diseases

Cells, Journal Year: 2023, Volume and Issue: 12(21), P. 2528 - 2528

Published: Oct. 26, 2023

DNA methylation is an epigenetic mechanism that regulates gene expression without altering sequences in health and disease. methyltransferases (DNMTs) are enzymes responsible for methylation, their dysregulation both a pathogenic of disease therapeutic target. DNMTs change by methylating CpG islands within exonic intergenic regions, which typically reduces transcription. Initially, mutations the DNMT genes pathologic protein were found to cause hematologic diseases, like myeloproliferative acute myeloid leukemia, but recently they have been shown promote cardiovascular including coronary artery pulmonary hypertension. We reviewed regulation functions DNMTs, with emphasis on somatic DNMT3A, common clonal hematopoiesis indeterminant potential (CHIP) may also be involved development arterial hypertension (PAH). Accumulation DNMT3A other CHIP hematopoietic cells tissues creates inflammatory environment promotes cardiopulmonary even absence This review summarized current understanding roles maintenance de novo contribute pathogenesis PAH.

Language: Английский

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The Effect of Extracellular Vesicles on Thrombosis

Journal of Cardiovascular Translational Research, Journal Year: 2022, Volume and Issue: 16(3), P. 682 - 697

Published: Nov. 28, 2022

The risk of cardiovascular events caused by acute thrombosis is high, including myocardial infarction, stroke, pulmonary embolism, and deep vein thrombosis. In this review, we summarize the roles extracellular vesicles different cellular origins in various associated with thrombosis, as described current literature, to facilitate future development a precise therapy for such vesicles. We hope that our review will indicate new horizon field research regard treatment especially targeting secreted individual cells. As more emerging technologies are being developed, diagnostic therapeutic strategies related EVs expected be identified diseases future.

Language: Английский

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