Influence of selected plant extracts on bacterial motility, aggregation, hydrophobicity, exopolysaccharide production and quorum sensing during biofilm formation of enterohaemorrhagic Escherichia coli O157:H7 DOI Creative Commons

M.M. Lebeloane,

Ibukun M. Famuyide, Jean Paul Dzoyem

et al.

South African Journal of Botany, Journal Year: 2024, Volume and Issue: 167, P. 197 - 208

Published: Feb. 20, 2024

Diarrhoeagenic Escherichia coli is a serious threat to human and animal health, with antimicrobial drug resistance contributing the problem. Alternative mechanisms of prevention treatment E. coli-induced diarrhoea include those targeting biofilm production quorum sensing, properties that have been associated some investigated plant extracts. Plants were selected from various genera, including Vachellia, Senegalia, Morus, Leucaena, Salix, Grewia, Ziziphus, Searsia, Dichrostachys Ceratonia, based on their known activity. The antibacterial effects leaf extracts against multidrug-resistant O157:H7 using broth microdilution method, cytotoxicity normal mammalian cell lines was studied tetrazolium colorimetric assay. Subsequently, antibiofilm activity inhibition extracellular polymeric substance (EPS) by crystal violet as staining dye after 0 24 h incubation carbohydrate estimation 5 % phenol-sulfuric biosensor strain Chromobacterium violaceum ATCC 12472 used study anti-quorum sensing potential Cell motility, surface hydrophobicity (CSH), aggregation also investigated. All had weak (MIC > 0.62 mg/ml) relatively low cytotoxicity. acetone Salix babylonica Leucaena leucocephala prevented bacterial attachment (0 h) 81.21 89.36 %, respectively. Approximately 28 eradicated established biofilms more than 50 however, they unable inhibit EPS above 30 %. half-maximal concentration generally inhibited violacein (ranging 0.01 0.02 mg/ml), V. erioloba extract being most effective at quenching. Some exhibited significant difference (p<0.05) in compared untreated cells. This highlights act via different action decrease virulence enteric coli, motivating further investigation bioactive compounds leaves.

Language: Английский

Microbial Biofilm: A Review on Formation, Infection, Antibiotic Resistance, Control Measures, and Innovative Treatment DOI Creative Commons

Satish J. Sharma,

James L. Mohler, Supriya D. Mahajan

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(6), P. 1614 - 1614

Published: June 19, 2023

Biofilm is complex and consists of bacterial colonies that reside in an exopolysaccharide matrix attaches to foreign surfaces a living organism. frequently leads nosocomial, chronic infections clinical settings. Since the bacteria biofilm have developed antibiotic resistance, using antibiotics alone treat brought on by ineffective. This review provides succinct summary theories behind composition of, formation drug-resistant attributed cutting-edge curative approaches counteract biofilm. The high frequency medical device-induced due warrants application innovative technologies manage complexities presented

Language: Английский

Citations

375
Bacterial resistance to antibacterial agents: Mechanisms, control strategies, and implications for global health DOI Creative Commons
Ting Li, Zhenlong Wang, Jianhua Guo

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 860, P. 160461 - 160461

Published: Nov. 24, 2022

Language: Английский

Citations

89
Modern Developments in the Application and Function of Metal/Metal Oxide Nanocomposite–Based Antibacterial Agents DOI
Yasser Fakri Mustafa

BioNanoScience, Journal Year: 2023, Volume and Issue: 13(2), P. 840 - 852

Published: April 15, 2023

Language: Английский

Citations

65
Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on Quorum Sensing (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination DOI Open Access
Edyta Juszczuk‐Kubiak

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2655 - 2655

Published: Feb. 24, 2024

One of the key mechanisms enabling bacterial cells to create biofilms and regulate crucial life functions in a global highly synchronized way is communication system called quorum sensing (QS). QS cell-to-cell process that depends on population density mediated by small signalling molecules autoinducers (AIs). In bacteria, controls biofilm formation through regulation gene expression involved extracellular polymeric matrix (EPS) synthesis, virulence factor production, stress tolerance metabolic adaptation. Forming one antimicrobial resistance (AMR). A common feature human pathogens ability form biofilm, which poses serious medical issue due their high susceptibility traditional antibiotics. Because associated with formation, there belief inhibition activity quenching (QQ) may provide alternative therapeutic methods for treating microbial infections. This review summarises recent progress research, focusing biofilms, especially those formed pathogenic become resistant antibiotic treatment. Subsequently, potential approach highlighting innovative non-antibiotic strategies control AMR bacteria has been discussed.

Language: Английский

Citations

27
Investigating Biofilms: Advanced Methods for Comprehending Microbial Behavior and Antibiotic Resistance DOI Creative Commons
Ahmad Almatroudi

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(4), P. 133 - 133

Published: March 29, 2024

Biofilms, which consist of microorganisms enclosed in an extracellular polymeric material (EPS), hold immense importance the fields environmental research, industry, and medicine. They play a significant role ecosystem dynamics stability, but they also pose issues such as biofouling, corrosion, pollution. Biofilms medical environments are linked to persistent infections elevated healthcare expenses. The EPS matrix plays crucial maintaining structural integrity antibiotic resistance these structures. research primarily investigates facilitating horizontal gene transfer among biofilm communities, with particular emphasis on its impact this process. process is recognized pivotal mechanism emergence resistance, underscoring function biofilms. analysis highlights financial constraints caused by biofilms several industries. Biofilm-associated sector result escalated treatment expenses extended hospitalization periods. In industrial context, have increasing maintenance product contamination, emphasizing need for efficient management solutions. This review presents most recent progress utilization sophisticated imaging tools molecular methodologies. addition conventional techniques, explores tools, DNA RNA sequencing, conjunction proteomics. These approaches essential assessing genetic metabolic mechanisms that regulate development resistance. underscores significance employing interdisciplinary methodology study By incorporating range approaches, analysis, comprehensive understanding may be achieved. approach opens up possibilities developing novel solutions address negative impacts health, environment.

Language: Английский

Citations

17
Copper‐Based Nanozymes: Potential Therapies for Infectious Wounds DOI Open Access
Haojie Ge, Min Wang, Xiaolong Wei

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

Abstract Bacterial infections are a significant obstacle to the healing of acute and chronic wounds, such as diabetic ulcers burn injuries. Traditional antibiotics primary treatment for bacterial infections, but they present issues antibiotic resistance, limited efficacy, potential side effects. This challenge leads exploration nanozymes alternative therapeutic agents. Nanozymes nanomaterials with enzyme‐like activities. Owing their low production costs, high stability, scalability, multifunctionality, have emerged prominent focus in antimicrobial research. Among various types nanozymes, metal‐based offer several benefits, including broad‐spectrum activity robust catalytic properties. Specifically, copper‐based (CuNZs) shown considerable promoting wound healing. They exhibit strong effects, reduce inflammation, enhance tissue regeneration, making them highly advantageous use care. review describes dual functions CuNZs combating infection facilitating repair. Recent advancements design synthesis CuNZs, evaluating promotion, biosafety both vitro vivo on basis core components, critically important.

Language: Английский

Citations

2
Coumarins from carcinogenic phenol: synthesis, characterization, in silico, biosafety, anticancer, antioxidant, and anti-inflammatory assessments DOI
Yasser Fakri Mustafa

Chemical Papers, Journal Year: 2023, Volume and Issue: 78(1), P. 493 - 504

Published: Oct. 9, 2023

Language: Английский

Citations

41
Unleashing the power of polymeric nanoparticles — Creative triumph against antibiotic resistance: A review DOI Creative Commons
Gaurisha Alias Resha Ramnath Naik,

Amrita Arup Roy,

Srinivas Mutalik

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134977 - 134977

Published: Aug. 24, 2024

Antibiotic resistance (ABR) poses a universal concern owing to the widespread use of antibiotics in various sectors. Nanotechnology emerges as promising solution combat ABR, offering targeted drug delivery, enhanced bioavailability, reduced toxicity, and stability. This comprehensive review explores concepts antibiotic resistance, its mechanisms, multifaceted approaches ABR. The provides an in-depth exploration polymeric nanoparticles advanced delivery systems, focusing on strategies for targeting microbial infections contributing fight against Nanoparticles revolutionize antimicrobial approaches, emphasizing passive active targeting. role molecules, including small peptides, proteins, carbohydrates, stimuli-responsive is being explored recent research works. complex comprehension mechanisms ABR strategic nanotechnology present avenue advancing tactics, ensuring treatment efficacy, minimizing toxic effects, mitigating development Polymeric nanoparticles, derived from natural or synthetic polymers, are crucial overcoming Natural polymers like chitosan alginate exhibit inherent antibacterial properties, while such polylactic acid (PLA), polyethylene glycol (PEG), polycaprolactone (PCL) can be engineered specific effects. study valuable source information researchers, healthcare professionals, policymakers engaged urgent quest overcome

Language: Английский

Citations

14
Antifungal drug resistance in Candida: a special emphasis on amphotericin B DOI

Lailema Ahmady,

Manisha Gothwal,

Muhammed Mushthaque Mukkoli

et al.

Apmis, Journal Year: 2024, Volume and Issue: 132(5), P. 291 - 316

Published: March 11, 2024

Invasive fungal infections in humans caused by several Candida species, increased considerably immunocompromised or critically ill patients, resulting substantial morbidity and mortality. albicans is the most prevalent although frequency of these organisms varies greatly according to geographic region. Infections with C. non‐albicans species have become more common, especially past 20 years, as a result aging, immunosuppressive medication use, endocrine disorders, malnourishment, extended use medical equipment, an increase immunogenic diseases. Despite being frequently associated human infections, glabrata , parapsilosis tropicalis krusei also been identified. Several antifungal drugs different modes action are approved for clinical settings treat infections. However, due common eukaryotic structure fungi, only limited number available therapeutic use. Furthermore, drug resistance has emerged growing currently against Amphotericin B (AmB), polyene class drugs, mainly used treatment serious systemic AmB interacts plasma membrane ergosterol, triggering cellular ion leakage via pore formation, extracting ergosterol from inducing death. primarily changes content ergosterol. This review summarizes exhibited special focus on AmB.

Language: Английский

Citations

13
Synthesis and evaluation of novel ring-conjugated coumarins as biosafe broad-spectrum antimicrobial candidates DOI
Nameer Mazin Zeki, Yasser Fakri Mustafa

Journal of Molecular Structure, Journal Year: 2024, Volume and Issue: 1309, P. 138192 - 138192

Published: March 30, 2024

Language: Английский

Citations

12