Molecular Reproduction and Development,
Journal Year:
2021,
Volume and Issue:
88(6), P. 416 - 426
Published: May 19, 2021
Abstract
The
effect
of
inbreeding
depression
on
sperm
motility
is
well
documented,
but
its
influence
morphometry
has
been
scarcely
examined
to
date.
Here,
we
combined
the
use
computer‐assisted
analysis
(CASMA)
with
a
SNP‐based
genomic
approach
determine
and
characterize
shape
highly
inbred
cattle
population.
We
determined
seven
morphometric
parameters
frozen‐thawed
samples
57
Retinta
bulls:
length
(L,
µm),
width
(W,
area
(A,
µm
2
),
perimeter
(P,
ellipticity
(ELI;
L/W),
elongation
(L−W)/(L
+
W)
perimeter‐to‐area
factor
(p2a;
P
/4
×
π
A).
comparison
(HI)
lowly
inbreed
(LI)
individuals
based
runs
homozygosity
(ROH)
values
(
F
ROH
)
showed
no
differences
between
groups.
An
additional
two‐step
unsupervised
subpopulation
significant
in
abundance
different
subpopulations
groups
p
<
0.05).
This
revealed
that
HI
bulls
harbored
higher
percentage
narrow‐head
as
opposed
large‐
round‐headed
detected
LI.
A
further
characterization
23
regions
differentially
affected
by
both
groups,
detecting
six
genes
SPAG6,
ARMC3,
PARK7,
VAMP3,
DYNLRB2,
PHF7)
previously
related
spermatogenesis‐associated
processes.
Molecular Reproduction and Development,
Journal Year:
2020,
Volume and Issue:
87(6), P. 633 - 649
Published: May 16, 2020
Abstract
Sperm
cells
of
all
taxa
share
a
common
goal
to
reach
and
fertilize
an
ovum,
yet
sperm
are
one
the
most
diverse
cell
types
in
nature.
While
structural
diversity
these
is
well
recognized,
functional
significance
variation
design
remains
elusive.
An
important
function
spermatozoa
need
migrate
toward
ova,
often
over
long
distances
foreign
environment,
which
may
include
complex
hostile
female
reproductive
tract.
Several
comparative
experimental
studies
have
attempted
address
link
between
morphology
motility,
conclusions
drawn
from
inconsistent,
even
within
same
taxa.
Much
what
we
know
about
internally
fertilizing
species
has
been
gleaned
vitro
studies,
for
parameters
vary
among
studies.
We
propose
that
discordant
results
part
due
lack
consistency
methods,
conditions
do
not
replicate
those
tract,
overuse
simple
linear
measures
shape.
Within
this
review,
provide
toolkit
imaging,
quantifying,
analyzing
movement
patterns
discuss
emerging
approaches.
Results
linking
motility
enhance
our
understanding
evolution
adaptive
traits
mechanisms
regulate
fertility,
thus
offering
new
insights
into
methods
used
assisted
technologies
animal
science,
conservation
public
health.
Revista de Biología Tropical,
Journal Year:
2019,
Volume and Issue:
67(6)
Published: Nov. 6, 2019
Sperm
kinematics
and
morphometric
subpopulations
analysis
with
CASA
systems:
a
review.
The
subjective
evaluation
of
seminal
quality
has
given
way
to
the
use
objective
assessment
techniques
by
technology
(computer-assisted
semen
analysis).
application
principal
components
(PC)
clustering
methods
reveal
spermatozoa
is
powerful
tool
evaluate
raw
processed
cell
suspensions,
but
not
many
researchers
are
aware
technique.
PC
multivariate
statistical
method
that
reduces
number
variables
used
in
subsequent
calculations
describe
data.
By
integrating
original
according
their
coherence
database
into
new
complex
mathematical
variable,
clearly
defined
homogenous
can
be
defined.
Kinematic,
morphometric,
morphological
or
DNA
integrity
tests
may
apply
characterize
it
understand
reproductive
biology
spermatozoon.
In
recent
years
there
been
substantive
change
conceptual
paradigm
regarding
what
an
ejaculate
is.
It
should
said
until
recently
was
considered
representative
population
(billions)
sperm
made
up
"equivalent"
cells
common
goal:
one
could
finally
fertilize
oocyte.
verification
set
grouped
distinct
subpopulations,
kinematic
characteristics,
opens
towards
more
cooperative
vision.
Besides,
we
have
seen
subpopulation
distribution
different
among
males,
which
seems
indicate
strategies
understood
within
another
paradigm,
competition
between
ejaculates.
existence
heterogeneous
show
patterns
widely
known
biological
meaning
these
still
clear.
Although
role
remains
unknown,
work
continue
direction.
[Objetive]
The
objective
of
the
present
study
was
to
characterize
different
sperm
subpopulations
based
on
morphometric
parameters
frozen-thawed
semen
in
two
bovine
subspecies
using
a
CASA
system.
[Methodology]
experiment
carried
out
at
Costa
Rica
Institute
Technology
from
May
December
2023.
Spermatozoa
10
bulls
(five
animals
each
species
Bos
taurus
and
indicus)
were
evaluated
after
thawing
doses
by
an
ISAS®v1,
computer-assisted
analysis
(CASA)-Morph
Sub-populations
head
spermatozoa
characterized
multivariate
procedures
such
as
principal
components
(PCs)
clustering
methods
(k-means
model)
[Results]
ejaculate
male
exhibits
significant
heterogeneity
comprises
diverse
with
differing
patterns.
Three
identified
three
PCs:
t
size,
shape,
symmetry
degree
which
it
pyriform.
proportions
varied
two-bovine
subspecies;
indicus.
Results
indicated
that
SP1,
SP2,
SP3
for
PC
criteria
these
differences
relevant.
variability
morphometry
assessments
underscores
need
standardize
evaluation
protocols
[Conclusions]
These
findings
highlight
importance
knowing
diversity
between
both
improve
vitro
tests
distinguish
subspecies.
Animals,
Journal Year:
2021,
Volume and Issue:
11(1), P. 227 - 227
Published: Jan. 18, 2021
Evaluation
of
sperm
morphometry
is
an
important
criterion
in
the
diagnosis
a
male
animal’s
suitability
for
breeding.
The
aim
study
was
to
evaluate
from
epididymides
dogs
subjected
routine
castration
using
various
staining
methods.
carried
out
on
semen
collected
ten
healthy
dogs.
Gonads
were
obtained
each
dog
during
at
veterinary
surgery.
Then,
(caput,
corpus,
cauda)
isolated
gonads,
them
and
microscope
slides
prepared.
evaluation
prepared
by
four
methods:
DiffQuik,
SpermBlue,
eosin-nigrosin
eosin-gentian.
A
total
2400
analyzed
(240
dog).
caput
corpus
epididymis
found
have
larger
heads
tails
than
those
cauda
epididymis.
method
shown
affect
taken
methods
differentiate
dimensions
head
different
parts
but
do
not
length
tail.
occurrence
differences
may
be
linked
use
fixatives
chemical
reagents
procedure.
Sperm
stained
EN
had
smallest
tail
dimensions.
greatest
area
noted
EG
method.
In
SB
method,
relatively
long
narrow.
used
are
suitable
structure,
possibility
all
enables
full
characterization
caput,
