Biotechnology Advances, Journal Year: 2024, Volume and Issue: 72, P. 108324 - 108324
Published: Feb. 13, 2024
Language: Английский
Advanced Materials, Journal Year: 2024, Volume and Issue: 36(33)
Published: June 27, 2024
Extracellular vesicles (EVs) are promising next-generation therapeutics and drug delivery systems due to demonstrated safety efficacy in preclinical models early-stage clinical trials. There is an urgent need address the immunogenicity of EVs (beyond apparent lack immunotoxicity) advance development. To date, few studies have assessed unintended immunological recognition EVs. An in-depth understanding EV-induced clearance necessary develop effective therapeutic strategies, including approaches mitigate when undesired. This article summarizes various factors involved potential strategies reduce for improved benefit.
Language: Английский
Citations
42
Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)
Published: June 14, 2024
Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.
Language: Английский
Citations
27
Sensors, Journal Year: 2024, Volume and Issue: 24(4), P. 1128 - 1128
Published: Feb. 8, 2024
Prostate cancer (PCa) is the second most common cancer. In this paper, isolation and properties of exosomes as potential novel liquid biopsy markers for early PCa diagnosis are investigated using two prostate human cell lines, i.e., benign (control) line RWPE1 carcinoma 22Rv1. Exosomes produced by both lines characterised various methods including nanoparticle-tracking analysis, dynamic light scattering, scanning electron microscopy atomic force microscopy. addition, surface plasmon resonance (SPR) used to study three different receptors on exosomal (CD63, CD81 prostate-specific membrane antigen-PMSA), implementing monoclonal antibodies identifying type glycans present lectins (glycan-recognising proteins). Electrochemical analysis understand interfacial exosomes. The results indicate that cancerous smaller, at higher concentrations, exhibit more nega tive zeta than control SPR experiments confirm negatively charged
Language: Английский
Citations
8
Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 5, 2024
Extracellular vesicles (EVs) harbor abundant glycans that mediate various functions, such as intercellular communication and disease advancement, which play significant roles in progression. However, the presence of EV heterogeneity body fluids complex nature glycan structures have posed challenges for detection glycans. In this study, we provide a streamlined method integrated,
Language: Английский
Citations
8
BMC Medicine, Journal Year: 2025, Volume and Issue: 23(1)
Published: Jan. 23, 2025
Language: Английский
Citations
1
Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(4)
Published: March 5, 2025
The review article provides a short introduction to exosomes with the focus use as disease markers itself (i.e. their concentration or presence of some specific receptors) source biomarkers such proteins and metabolites. In detail, we are discussing various methods exosome isolation main paper is on affinity capture exosomes, since them can be applied sub-populations produced by organs. comprehensive overview magnetic (bio)affinity detection exosomal cargo using different ligands antibodies, DNA aptamers, peptides, glycan-based recognition, transferrin-based approaches, based recognition phospholipids other approaches including electrostatic interactions. in detail key analytical clinical parameters form an extensive table summarising outcomes published last two years (2023-2024). Finally, also conclusions sections pros cons for and/or determination content.
Language: Английский
Citations
1
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 179, P. 117874 - 117874
Published: July 17, 2024
Language: Английский
Citations
7
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: Feb. 22, 2024
Gastric cancer (GC) is a malignant neoplasm originating from the epithelial cells of gastric mucosa. The pathogenesis GC intricately linked to tumor microenvironment within which reside. Tumor-associated macrophages (TAMs) primarily differentiate peripheral blood monocytes and can be broadly categorized into M1 M2 subtypes. M2-type TAMs have been shown promote growth, tissue remodeling, angiogenesis. Furthermore, they actively suppress acquired immunity, leading poorer prognosis reduced tolerance chemotherapy. Exosomes, contain myriad biologically active molecules including lipids, proteins, mRNA, noncoding RNAs, emerged as key mediators communication between TAMs. exchange these via exosomes markedly influence consequently impact progression. Recent studies elucidated correlation various clinicopathological parameters GC, such size, differentiation, infiltration depth, lymph node metastasis, TNM staging, highlighting pivotal role in development metastasis. In this review, we aim comprehensively examine bidirectional TAMs, implications alterations on immune escape, invasion, metastasis targeted therapeutic approaches for efficacy potential drug resistance strategies.
Language: Английский
Citations
6
Advanced Science, Journal Year: 2024, Volume and Issue: 11(31)
Published: June 18, 2024
Considerable progress has been made in the development of drug delivery systems for diabetic wounds. However, underlying drawbacks, such as low efficiency and poor tissue permeability, have rarely addressed. In this study, a multifunctional biohybrid nanorobot platform comprising an artificial unit several biological components is constructed. The magnetically driven surface modified with antibacterial 2-hydroxypropyltrimethyl ammonium chloride chitosan, which enables entire to move excellent penetration capacity. are two-step engineered extracellular vesicles that first loaded mangiferin, natural polyphenolic compound antioxidant properties, then glycoengineered on enhance cellular uptake efficiency. As expected, more easily absorbed by endothelial cells fibroblasts exhibits outstanding dermal performance properties. Encouraging results also observed infected wound models, showing improved re-epithelialization, collagen deposition, angiogenesis, accelerated healing. Collectively, possesses functionalities both units serves efficient system promote repair through dual-enhanced cell multistep interventions.
Language: Английский
Citations
5
Food Chemistry, Journal Year: 2024, Volume and Issue: 457, P. 140199 - 140199
Published: June 24, 2024
Language: Английский
Citations
5