The ever‐increasing necessity of mass spectrometry in dissecting protein post‐translational modifications catalyzed by bacterial effectors DOI Open Access
Jie Jin, Yi Yuan, Wei Xian

et al.

Molecular Microbiology, Journal Year: 2023, Volume and Issue: 119(6), P. 677 - 686

Published: May 1, 2023

Abstract Protein post‐translational modifications (PTMs), such as ADP‐ribosylation and phosphorylation, regulate multiple fundamental biological processes in cells. During bacterial infection, effector proteins are delivered into host cells through dedicated secretion systems can modulate important cellular pathways by covalently modifying their targets. These strategies enable intruding bacteria to subvert various processes, thereby promoting own survival proliferation. Despite rapid expansion of our understanding effector‐mediated PTMs cells, analytical measurements these molecular events still pose significant challenges the study host–pathogen interactions. Nevertheless, with major technical breakthroughs last two decades, mass spectrometry (MS) has evolved be a valuable tool for detecting protein mapping modification sites. Additionally, large‐scale PTM profiling, facilitated different enrichment prior MS analysis, allows high‐throughput screening enzymatic substrates effectors. In this review, we summarize advances studies representative (i.e., phosphorylation) catalyzed effectors during infection. Importantly, will discuss ever‐increasing role how latest MS‐based tools aid future booming area pathogenic bacteria–host

Language: Английский

Control of host PTMs by intracellular bacteria: An opportunity toward novel anti-infective agents DOI Creative Commons
Virginie Stévenin, Jacques Neefjes

Cell chemical biology, Journal Year: 2022, Volume and Issue: 29(5), P. 741 - 756

Published: May 1, 2022

Language: Английский

Citations

6
A natural compound hyperoside targetsSalmonellaTyphimurium T3SS needle protein InvG DOI
Yong Zhang, Yan Liu, Bo Zhang

et al.

Food & Function, Journal Year: 2022, Volume and Issue: 13(19), P. 9761 - 9771

Published: Jan. 1, 2022

After entry into the host cells, Salmonella Typhimurium creates a replication niche using its Type III system (T3SS). Hyperoside targets InvG to inhibit T3SS translocation of S. Typhimurium, thus inhibiting in cells.

Language: Английский

Citations

6
Legionella and mitochondria, an intriguing relationship DOI
Francisco J. García-Rodríguez, Carmen Buchrieser, Pedro Escoll

et al.

International review of cell and molecular biology, Journal Year: 2022, Volume and Issue: unknown, P. 37 - 81

Published: Nov. 18, 2022

Language: Английский

Citations

6
ADP-ribosylation: An emerging direction for disease treatment DOI
Yuting Liu, Yan Che,

Hong-liang Qiu

et al.

Ageing Research Reviews, Journal Year: 2023, Volume and Issue: 94, P. 102176 - 102176

Published: Dec. 21, 2023

Language: Английский

Citations

3
The ever‐increasing necessity of mass spectrometry in dissecting protein post‐translational modifications catalyzed by bacterial effectors DOI Open Access
Jie Jin, Yi Yuan, Wei Xian

et al.

Molecular Microbiology, Journal Year: 2023, Volume and Issue: 119(6), P. 677 - 686

Published: May 1, 2023

Abstract Protein post‐translational modifications (PTMs), such as ADP‐ribosylation and phosphorylation, regulate multiple fundamental biological processes in cells. During bacterial infection, effector proteins are delivered into host cells through dedicated secretion systems can modulate important cellular pathways by covalently modifying their targets. These strategies enable intruding bacteria to subvert various processes, thereby promoting own survival proliferation. Despite rapid expansion of our understanding effector‐mediated PTMs cells, analytical measurements these molecular events still pose significant challenges the study host–pathogen interactions. Nevertheless, with major technical breakthroughs last two decades, mass spectrometry (MS) has evolved be a valuable tool for detecting protein mapping modification sites. Additionally, large‐scale PTM profiling, facilitated different enrichment prior MS analysis, allows high‐throughput screening enzymatic substrates effectors. In this review, we summarize advances studies representative (i.e., phosphorylation) catalyzed effectors during infection. Importantly, will discuss ever‐increasing role how latest MS‐based tools aid future booming area pathogenic bacteria–host

Language: Английский

Citations

2