Trends in biotechnology,
Год журнала:
2024,
Номер
42(10), С. 1241 - 1257
Опубликована: Апрель 23, 2024
Advances
in
tissue
engineering
for
both
system
modeling
and
organ
regeneration
depend
on
embracing
recapitulating
the
target
tissue's
functional
structural
complexity.
Microenvironmental
features
such
as
anisotropy,
heterogeneity,
other
biochemical
mechanical
spatiotemporal
cues
are
essential
regulating
development
function.
Novel
biofabrication
strategies
innovative
biomaterial
design
have
emerged
promising
tools
to
better
reproduce
features.
These
facilitate
a
transition
towards
high-fidelity
biomimetic
structures,
offering
opportunities
deeper
understanding
of
function
superior
therapies.
In
this
review,
we
explore
some
key
compositional
aspects
tissues,
lay
out
how
achieve
similar
outcomes
with
current
fabrication
strategies,
identify
main
challenges
avenues
future
research.
Advanced Materials,
Год журнала:
2024,
Номер
36(34)
Опубликована: Июнь 11, 2024
The
repair
and
functional
reconstruction
of
bone
defects
resulting
from
severe
trauma,
surgical
resection,
degenerative
disease,
congenital
malformation
pose
significant
clinical
challenges.
Bone
tissue
engineering
(BTE)
holds
immense
potential
in
treating
these
defects,
without
incurring
prevalent
complications
associated
with
conventional
autologous
or
allogeneic
grafts.
3D
printing
technology
enables
control
over
architectural
structures
at
multiple
length
scales
has
been
extensively
employed
to
process
biomimetic
scaffolds
for
BTE.
In
contrast
inert
grafts,
next-generation
smart
possess
a
remarkable
ability
mimic
the
dynamic
nature
native
extracellular
matrix
(ECM),
thereby
facilitating
regeneration.
Additionally,
they
can
generate
tailored
controllable
therapeutic
effects,
such
as
antibacterial
antitumor
properties,
response
exogenous
and/or
endogenous
stimuli.
This
review
provides
comprehensive
assessment
progress
3D-printed
BTE
applications.
It
begins
an
introduction
physiology,
followed
by
overview
technologies
utilized
scaffolds.
Notable
advances
various
stimuli-responsive
strategies,
efficacy,
applications
are
discussed.
Finally,
highlights
existing
challenges
development
implementation
scaffolds,
well
emerging
this
field.
Nano-Micro Letters,
Год журнала:
2023,
Номер
15(1)
Опубликована: Окт. 31, 2023
Abstract
Blood
vessels
are
essential
for
nutrient
and
oxygen
delivery
waste
removal.
Scaffold-repairing
materials
with
functional
vascular
networks
widely
used
in
bone
tissue
engineering.
Additive
manufacturing
is
a
technology
that
creates
three-dimensional
solids
by
stacking
substances
layer
layer,
mainly
including
but
not
limited
to
3D
printing,
also
4D
5D
printing
6D
printing.
It
can
be
effectively
combined
vascularization
meet
the
needs
of
vascularized
scaffolds
precisely
tuning
mechanical
structure
biological
properties
smart
scaffolds.
Herein,
development
neovascularization
engineering
systematically
discussed
terms
importance
tissue.
Additionally,
research
progress
future
prospects
printed
scaffold
highlighted
presented
four
categories:
scaffolds,
cell-based
loaded
specific
carriers
bionic
Finally,
brief
review
additive
manufacturing-tissue
related
tissues
such
as
engineering,
cardiovascular
system,
skeletal
muscle,
soft
discussion
challenges
efforts
leading
significant
advances
intelligent
regeneration
presented.
Advanced Healthcare Materials,
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 15, 2023
Granular
hydrogels,
which
are
formed
by
densely
packing
microgels,
promising
materials
for
bioprinting
due
to
their
extrudability,
porosity,
and
modularity.
However,
the
multidimensional
parameter
space
involved
in
granular
hydrogel
design
makes
material
optimization
challenging.
For
example,
inputs
such
as
microgel
morphology,
density,
or
stiffness
can
influence
multiple
rheological
properties
that
govern
printability
behavior
of
encapsulated
cells.
This
review
provides
an
overview
fabrication
methods
then
examines
how
important
associated
with
cellular
responses
across
scales.
Recent
applications
principles
bioink
engineering
described,
including
development
support
hydrogels
embedded
printing.
Further,
paper
key
physical
responses,
highlighting
advantages
promoting
cell
tissue
maturation
after
printing
process.
Finally,
potential
future
directions
advancing
discussed.
Advanced Materials,
Год журнала:
2024,
Номер
36(34)
Опубликована: Янв. 20, 2024
Abstract
Volumetric
additive
manufacturing
(VAM)
is
an
emerging
layerless
method
for
the
rapid
processing
of
reactive
resins
into
3D
structures,
where
printing
much
faster
(seconds)
than
other
lithography
and
direct
ink
writing
methods
(minutes
to
hours).
As
a
vial
resin
rotates
in
VAM
process,
patterned
light
exposure
defines
object
then
that
has
not
undergone
gelation
can
be
washed
away.
Despite
promise
VAM,
there
are
challenges
with
soft
hydrogel
materials
from
non‐viscous
precursors,
including
multi‐material
constructs.
To
address
this,
sacrificial
gelatin
used
modulate
viscosity
support
cytocompatible
macromers
based
on
poly(ethylene
glycol)
(PEG),
hyaluronic
acid
(HA),
polyacrylamide
(PA).
After
printing,
removed
by
washing
at
elevated
temperature.
print
constructs,
gelatin‐containing
as
shear‐yielding
suspension
bath
(including
HA
further
properties)
extruded
define
processed
defined
object.
Multi‐material
constructs
methacrylated
(MeHA)
methacrylamide
(GelMA)
printed
(as
proof‐of‐concept)
encapsulated
mesenchymal
stromal
cells
(MSCs),
local
properties
guide
cell
spreading
behavior
culture.
Nano-Micro Letters,
Год журнала:
2024,
Номер
16(1)
Опубликована: Июнь 17, 2024
Abstract
Microgels
prepared
from
natural
or
synthetic
hydrogel
materials
have
aroused
extensive
attention
as
multifunctional
cells
drug
carriers,
that
are
promising
for
tissue
engineering
and
regenerative
medicine.
can
also
be
aggregated
into
microporous
scaffolds,
promoting
cell
infiltration
proliferation
repair.
This
review
gives
an
overview
of
recent
developments
in
the
fabrication
techniques
applications
microgels.
A
series
conventional
novel
strategies
including
emulsification,
microfluidic,
lithography,
electrospray,
centrifugation,
gas-shearing,
three-dimensional
bioprinting,
etc.
discussed
depth.
The
characteristics
microgels
microgel-based
scaffolds
culture
delivery
elaborated
with
emphasis
on
advantages
these
carriers
therapy.
Additionally,
we
expound
ongoing
foreseeable
current
limitations
their
aggregate
field
biomedical
engineering.
Through
stimulating
innovative
ideas,
present
paves
new
avenues
expanding
application
techniques.
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 5, 2025
Engineered
living
systems
(ELSs)
represent
purpose-driven
assemblies
of
components,
encompassing
cells,
biomaterials,
and
active
agents,
intricately
designed
to
fulfill
diverse
biomedical
applications.
Gelatin
its
derivatives
have
been
used
extensively
in
ELSs
owing
their
mature
translational
pathways,
favorable
biological
properties,
adjustable
physicochemical
characteristics.
This
review
explores
the
intersection
gelatin
with
fabrication
techniques,
offering
a
comprehensive
examination
synergistic
potential
creating
for
various
applications
biomedicine.
It
offers
deep
dive
into
gelatin,
including
structures
production,
sources,
processing,
properties.
Additionally,
techniques
employing
derivatives,
generic
microfluidics,
3D
printing
methods.
Furthermore,
it
discusses
based
on
regenerative
engineering
as
well
cell
therapies,
bioadhesives,
biorobots,
biosensors.
Future
directions
challenges
are
also
examined,
highlighting
emerging
trends
areas
improvements
innovations.
In
summary,
this
underscores
significance
gelatin-based
advancing
lays
groundwork
guiding
future
research
developments
within
field.
Bioactive Materials,
Год журнала:
2023,
Номер
32, С. 356 - 384
Опубликована: Окт. 21, 2023
Three-dimensional
bioprinting
is
an
advanced
tissue
fabrication
technique
that
allows
printing
complex
structures
with
precise
positioning
of
multiple
cell
types
layer-by-layer.
Compared
to
other
methods,
extrusion
has
several
advantages
print
large-sized
constructs
and
organ
models
due
large
build
volume.
Extrusion
using
sacrificial,
support
embedded
strategies
have
been
successfully
employed
facilitate
hollow
structures.
Embedded
a
gel-in-gel
approach
developed
overcome
the
gravitational
overhanging
limits
micron-scale
resolution.
In
bioprinting,
deposition
bioinks
into
microgel
or
granular
bath
will
be
facilitated
by
sol-gel
transition
through
needle
movement
inside
medium.
This
review
outlines
various
polymers
used
in
systems
advantages,
limitations,
efficacy
vascularized
tissues
Further,
essential
requirements
like
viscoelasticity,
stability,
transparency
easy
extraction
human
scale
organs
are
discussed.
Additionally,
geometries
vascular
constructs,
heart,
bone,
octopus
jellyfish
printed
assisted
methods
their
anatomical
features
elaborated.
Finally,
challenges
clinical
translation
future
scope
these
replace
native
envisaged.
Biofabrication,
Год журнала:
2023,
Номер
16(1), С. 012004 - 012004
Опубликована: Ноя. 3, 2023
The
three-dimensional
(3D)
bioprinting
technologies
are
suitable
for
biomedical
applications
owing
to
their
ability
manufacture
complex
and
high-precision
tissue
constructs.
However,
the
slow
printing
speed
of
current
layer-by-layer
(bio)printing
modality
is
major
limitation
in
biofabrication
field.
To
overcome
this
issue,
volumetric
(VBP)
developed.
VBP
changes
layer-wise
operation
conventional
devices,
permitting
creation
geometrically
complex,
centimeter-scale
constructs
tens
seconds.
next
step
onward
from
sequential
methods,
opening
new
avenues
fast
additive
manufacturing
fields
engineering,
regenerative
medicine,
personalized
drug
testing,
soft
robotics,
etc.
Therefore,
review
introduces
principles
hardware
designs
VBP-based
techniques;
then
focuses
on
recent
advances
(bio)inks
applications.
Lastly,
limitations
discussed
together
with
future
direction
research.
Organ
damage
or
failure
arising
from
injury,
disease,
and
aging
poses
challenges
due
to
the
body's
limited
regenerative
capabilities.
transplantation
presents
issues
of
donor
shortages
immune
rejection
risks,
necessitating
innovative
solutions.
The
3D
bioprinting
organs
on
demand
offers
promise
in
tissue
engineering
medicine.
In
this
review,
we
explore
state-of-the-art
technologies,
with
a
focus
bioink
cell
type
selections.
We
follow
discussions
advances
solid
organs,
such
as
heart,
liver,
kidney,
pancreas,
highlighting
importance
vascularization
integration.
Finally,
provide
insights
into
key
future
directions
context
clinical
translation
bioprinted
their
large-scale
production.
