Nature Reviews Bioengineering, Год журнала: 2024, Номер unknown
Опубликована: Сен. 26, 2024
Язык: Английский
Advanced Materials, Год журнала: 2024, Номер 36(34)
Опубликована: Июнь 11, 2024
The repair and functional reconstruction of bone defects resulting from severe trauma, surgical resection, degenerative disease, congenital malformation pose significant clinical challenges. Bone tissue engineering (BTE) holds immense potential in treating these defects, without incurring prevalent complications associated with conventional autologous or allogeneic grafts. 3D printing technology enables control over architectural structures at multiple length scales has been extensively employed to process biomimetic scaffolds for BTE. In contrast inert grafts, next-generation smart possess a remarkable ability mimic the dynamic nature native extracellular matrix (ECM), thereby facilitating regeneration. Additionally, they can generate tailored controllable therapeutic effects, such as antibacterial antitumor properties, response exogenous and/or endogenous stimuli. This review provides comprehensive assessment progress 3D-printed BTE applications. It begins an introduction physiology, followed by overview technologies utilized scaffolds. Notable advances various stimuli-responsive strategies, efficacy, applications are discussed. Finally, highlights existing challenges development implementation scaffolds, well emerging this field.
Язык: Английский
Процитировано
65
Nano-Micro Letters, Год журнала: 2023, Номер 15(1)
Опубликована: Окт. 31, 2023
Abstract Blood vessels are essential for nutrient and oxygen delivery waste removal. Scaffold-repairing materials with functional vascular networks widely used in bone tissue engineering. Additive manufacturing is a technology that creates three-dimensional solids by stacking substances layer layer, mainly including but not limited to 3D printing, also 4D 5D printing 6D printing. It can be effectively combined vascularization meet the needs of vascularized scaffolds precisely tuning mechanical structure biological properties smart scaffolds. Herein, development neovascularization engineering systematically discussed terms importance tissue. Additionally, research progress future prospects printed scaffold highlighted presented four categories: scaffolds, cell-based loaded specific carriers bionic Finally, brief review additive manufacturing-tissue related tissues such as engineering, cardiovascular system, skeletal muscle, soft discussion challenges efforts leading significant advances intelligent regeneration presented.
Язык: Английский
Процитировано
56
Advanced Materials, Год журнала: 2024, Номер 36(34)
Опубликована: Янв. 20, 2024
Abstract Volumetric additive manufacturing (VAM) is an emerging layerless method for the rapid processing of reactive resins into 3D structures, where printing much faster (seconds) than other lithography and direct ink writing methods (minutes to hours). As a vial resin rotates in VAM process, patterned light exposure defines object then that has not undergone gelation can be washed away. Despite promise VAM, there are challenges with soft hydrogel materials from non‐viscous precursors, including multi‐material constructs. To address this, sacrificial gelatin used modulate viscosity support cytocompatible macromers based on poly(ethylene glycol) (PEG), hyaluronic acid (HA), polyacrylamide (PA). After printing, removed by washing at elevated temperature. print constructs, gelatin‐containing as shear‐yielding suspension bath (including HA further properties) extruded define processed defined object. Multi‐material constructs methacrylated (MeHA) methacrylamide (GelMA) printed (as proof‐of‐concept) encapsulated mesenchymal stromal cells (MSCs), local properties guide cell spreading behavior culture.
Язык: Английский
Процитировано
34
Advanced Materials, Год журнала: 2025, Номер unknown
Опубликована: Фев. 5, 2025
Engineered living systems (ELSs) represent purpose-driven assemblies of components, encompassing cells, biomaterials, and active agents, intricately designed to fulfill diverse biomedical applications. Gelatin its derivatives have been used extensively in ELSs owing their mature translational pathways, favorable biological properties, adjustable physicochemical characteristics. This review explores the intersection gelatin with fabrication techniques, offering a comprehensive examination synergistic potential creating for various applications biomedicine. It offers deep dive into gelatin, including structures production, sources, processing, properties. Additionally, techniques employing derivatives, generic microfluidics, 3D printing methods. Furthermore, it discusses based on regenerative engineering as well cell therapies, bioadhesives, biorobots, biosensors. Future directions challenges are also examined, highlighting emerging trends areas improvements innovations. In summary, this underscores significance gelatin-based advancing lays groundwork guiding future research developments within field.
Язык: Английский
Процитировано
3
Bioactive Materials, Год журнала: 2025, Номер 48, С. 273 - 293
Опубликована: Фев. 20, 2025
Язык: Английский
Процитировано
3
Advanced Healthcare Materials, Год журнала: 2023, Номер unknown
Опубликована: Июнь 15, 2023
Granular hydrogels, which are formed by densely packing microgels, promising materials for bioprinting due to their extrudability, porosity, and modularity. However, the multidimensional parameter space involved in granular hydrogel design makes material optimization challenging. For example, inputs such as microgel morphology, density, or stiffness can influence multiple rheological properties that govern printability behavior of encapsulated cells. This review provides an overview fabrication methods then examines how important associated with cellular responses across scales. Recent applications principles bioink engineering described, including development support hydrogels embedded printing. Further, paper key physical responses, highlighting advantages promoting cell tissue maturation after printing process. Finally, potential future directions advancing discussed.
Язык: Английский
Процитировано
43
Bioactive Materials, Год журнала: 2023, Номер 32, С. 356 - 384
Опубликована: Окт. 21, 2023
Three-dimensional bioprinting is an advanced tissue fabrication technique that allows printing complex structures with precise positioning of multiple cell types layer-by-layer. Compared to other methods, extrusion has several advantages print large-sized constructs and organ models due large build volume. Extrusion using sacrificial, support embedded strategies have been successfully employed facilitate hollow structures. Embedded a gel-in-gel approach developed overcome the gravitational overhanging limits micron-scale resolution. In bioprinting, deposition bioinks into microgel or granular bath will be facilitated by sol-gel transition through needle movement inside medium. This review outlines various polymers used in systems advantages, limitations, efficacy vascularized tissues Further, essential requirements like viscoelasticity, stability, transparency easy extraction human scale organs are discussed. Additionally, geometries vascular constructs, heart, bone, octopus jellyfish printed assisted methods their anatomical features elaborated. Finally, challenges clinical translation future scope these replace native envisaged.
Язык: Английский
Процитировано
37
Biofabrication, Год журнала: 2023, Номер 16(1), С. 012004 - 012004
Опубликована: Ноя. 3, 2023
The three-dimensional (3D) bioprinting technologies are suitable for biomedical applications owing to their ability manufacture complex and high-precision tissue constructs. However, the slow printing speed of current layer-by-layer (bio)printing modality is major limitation in biofabrication field. To overcome this issue, volumetric (VBP) developed. VBP changes layer-wise operation conventional devices, permitting creation geometrically complex, centimeter-scale constructs tens seconds. next step onward from sequential methods, opening new avenues fast additive manufacturing fields engineering, regenerative medicine, personalized drug testing, soft robotics, etc. Therefore, review introduces principles hardware designs VBP-based techniques; then focuses on recent advances (bio)inks applications. Lastly, limitations discussed together with future direction research.
Язык: Английский
Процитировано
26
Nano-Micro Letters, Год журнала: 2024, Номер 16(1)
Опубликована: Июнь 17, 2024
Abstract Microgels prepared from natural or synthetic hydrogel materials have aroused extensive attention as multifunctional cells drug carriers, that are promising for tissue engineering and regenerative medicine. can also be aggregated into microporous scaffolds, promoting cell infiltration proliferation repair. This review gives an overview of recent developments in the fabrication techniques applications microgels. A series conventional novel strategies including emulsification, microfluidic, lithography, electrospray, centrifugation, gas-shearing, three-dimensional bioprinting, etc. discussed depth. The characteristics microgels microgel-based scaffolds culture delivery elaborated with emphasis on advantages these carriers therapy. Additionally, we expound ongoing foreseeable current limitations their aggregate field biomedical engineering. Through stimulating innovative ideas, present paves new avenues expanding application techniques.
Язык: Английский
Процитировано
17
Engineering, Год журнала: 2024, Номер unknown
Опубликована: Июнь 1, 2024
Organ damage or failure arising from injury, disease, and aging poses challenges due to the body's limited regenerative capabilities. transplantation presents issues of donor shortages immune rejection risks, necessitating innovative solutions. The 3D bioprinting organs on demand offers promise in tissue engineering medicine. In this review, we explore state-of-the-art technologies, with a focus bioink cell type selections. We follow discussions advances solid organs, such as heart, liver, kidney, pancreas, highlighting importance vascularization integration. Finally, provide insights into key future directions context clinical translation bioprinted their large-scale production.
Язык: Английский
Процитировано
12