Biomaterials Advances, Год журнала: 2023, Номер 154, С. 213580 - 213580
Опубликована: Авг. 6, 2023
Язык: Английский
npj Regenerative Medicine, Год журнала: 2022, Номер 7(1)
Опубликована: Дек. 22, 2022
Abstract Reconstruction of complex cartilage defects has remained a great challenge for tissue engineering due to the lack stem cells and chronic inflammation within joint. In this study, we have developed an injectable pig cartilage-derived decellularized extracellular matrix (dECM) hydrogels repair defects, which shown sound biocompatibility immunomodulatory capacity both in vitro vivo. The dECM can enhance chondrogenic differentiation human urine-derived (USCs). As by experiment, USCs survived, proliferated, produced mass cartilage-specific containing collagen II aggrecan. And USCs-laden promote secretion matrix, modulate immune response regeneration rat model defect.
Язык: Английский
Процитировано
44
Advanced Materials, Год журнала: 2023, Номер 36(3)
Опубликована: Окт. 20, 2023
Abstract Chronic wounds caused by bacterial infections are a major challenge in medical fields. The hypoxia condition extremely induces reactive oxygen species (ROS) generation and upregulates the expression of hypoxia‐inducible factor, both which can increase pro‐inflammatory M1 subtype macrophages production while reducing anti‐inflammatory M2 macrophages. Besides, bacteria‐formed biofilms hinder penetration therapeutic agents. Encouraged natural motors automatically executing tasks, hypothesized that supplying sufficient (O 2 ) would simultaneously drive agent movement, rescue hypoxic microenvironment, disrupt vicious cycle inflammation. Here, small organic molecule‐based nanoparticles (2TT‐mC6B@Cu 5.4 O NPs) possess high photothermal conversion efficiency enzymatic activities developed, including superoxide dismutase‐, catalase‐, glutathione peroxidase‐like activity. 2TT‐mC6B@Cu NPs exhibit superior ROS‐scavenging abilities synergistically relieve inflammation, alleviate conditions, promote their deep chronic wound tissues. Transcriptome analysis further demonstrates 2TT‐[email protected] inhibit biological inside bacteria. Furthermore, vivo experiments prove NPs‐based hyperthermia effectively eliminate bacteria to healing.
Язык: Английский
Процитировано
36
Pharmaceutics, Год журнала: 2023, Номер 15(5), С. 1334 - 1334
Опубликована: Апрель 25, 2023
With the in-depth understanding of bone regeneration mechanisms and development tissue engineering, a variety scaffold carrier materials with desirable physicochemical properties biological functions have recently emerged in field regeneration. Hydrogels are being increasingly used engineering because their biocompatibility, unique swelling properties, relative ease fabrication. Hydrogel drug delivery systems comprise cells, cytokines, an extracellular matrix, small molecule nucleotides, which different depending on chemical or physical cross-linking. Additionally, hydrogels can be designed for types specific applications. In this paper, we summarize recent research using as carriers, detail application defect diseases mechanisms, discuss future directions hydrogel engineering.
Язык: Английский
Процитировано
32
Advanced Functional Materials, Год журнала: 2023, Номер 33(51)
Опубликована: Авг. 11, 2023
Abstract The high density structure of cartilage matrix negative charge seriously hinders the penetration and enrichment drugs/genes into tissue. In this study, hexachlorocyclotriphosphonitrile is used as core to prepare self‐polymerized phosphorus dendrimers with inflammatory reaction through substitution condensation reaction. vector can achieve efficient damaged articular (slightly acidic condition) effective anchoring (normal physiological size effect under response. Meanwhile, a gene “delivery library,” G1‐NC5.HCl@siRNA gradually released microspheres degrading; long‐term silencing specific genes realized. Further, loaded hydrogel by ionic bond coordination microfluidic techniques improve residency in joint cavity. results vitro vivo e xperiments show that complex has better at pH = 6.6, decreases 7.6, which effectively anchor site for continuous drug delivery. composite significantly degeneration osteoarthritis (OA) promote regeneration. Therefore, microsphere delivery system response promising penetrant/anchoring carrier OA treatment.
Язык: Английский
Процитировано
23
Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)
Опубликована: Июнь 26, 2024
Abstract Tissue regeneration technology has been rapidly developed and widely applied in tissue engineering repair. Compared with traditional approaches like surgical treatment, the rising gene therapy is able to have a durable effect on regeneration, such as impaired bone articular cartilage repair cancer-resected Gene can also facilitate production of situ therapeutic factors, thus minimizing diffusion or loss complexes enabling spatiotemporally controlled release products for regeneration. Among different delivery vectors supportive gene-activated matrices, advanced gene/drug nanocarriers attract exceptional attraction due their tunable physiochemical properties, well excellent adaptive performance bone, cartilage, blood vessel, nerve This paper reviews recent advances nonviral-mediated systems an emphasis important role
Язык: Английский
Процитировано
15
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 268, С. 131643 - 131643
Опубликована: Апрель 21, 2024
Язык: Английский
Процитировано
14
BMEMat, Год журнала: 2024, Номер unknown
Опубликована: Июнь 10, 2024
Abstract Dental and orthopedic titanium implants are successfully widely used but still face challenges due to complications leading high treatment cost, morbidity, even mortality. This review focuses on the hybrid coatings designed prevent mitigate implant failure by integrating multiple strategies materials. The forms of manufacturing synthesizing were first discussed. We then categorize these based their biological functions: antibacterial coatings, which essential for preventing difficult‐to‐treat infection; promote osseointegration, crucial mechanical stability implants; that encourage soft tissue attachment, contributing overall success esthetics implant. summarize state art in multifunctional integrate functions as an alternative, holistic approach reducing complications. culminates a discussion future directions field, emphasizing potential notable biofunctional toward obtaining commercial patients. Together, our article provides comprehensive overview current developments glimpse into potentially revolutionizing dental implants.
Язык: Английский
Процитировано
12
Frontiers in Immunology, Год журнала: 2024, Номер 14
Опубликована: Янв. 9, 2024
Osteoarthritis (OA) has been a leading cause of disability in the elderly and there remains lack effective therapeutic approaches as mechanisms pathogenesis progression have yet to be elucidated. As OA progresses, cellular metabolic profiles energy production are altered, emerging reprogramming highlights importance specific pathways disease progression. crucial part glucose metabolism, glycolysis bridges inflammatory dysfunctions. Moreover, glycolytic pathway is involved different areas metabolism inflammation, associated with variety transcription factors. To date, it not fully elucidated whether changes its key enzymes onset or OA. This review summarizes important role mediating inducing tissue inflammation injury, aim providing further insights into pathological functions proposing new targets for treatment
Язык: Английский
Процитировано
11
Advanced Functional Materials, Год журнала: 2024, Номер 34(16)
Опубликована: Янв. 3, 2024
Abstract The foundation of regenerative medicine is the investigation and progression hypotheses approaches for substituting, mending, reconstructing, or regenerating different tissues organs within human body. Nevertheless, due to slender central segment entire unattached configuration meniscus, efficacy conventional therapies unsatisfactory in reinstating meniscal health functionality. To overcome these limitations, medical‐type hydrogels have potential provide a solution meniscus repair regeneration. Their biocompatibility modifiability enable safe implantations vivo, effectively modifying pathophysiology cells surrounding microenvironment at site injury. This article reviews usage implantable regeneration repair. First, structure physiologic function are first briefly described. Following this, use varying highlighted, alongside discussion materials employed constructing hydrogel implants. Lastly, an analysis issues challenges associated with presented.
Язык: Английский
Процитировано
8
Biofabrication, Год журнала: 2025, Номер 17(1), С. 015044 - 015044
Опубликована: Янв. 1, 2025
Tissue-engineered grafts that mimic articular cartilage show promise for treating injuries. However, engineering cell-based therapies to match zonal architecture and biochemical composition remains challenging. Decellularized extracellular matrix (dECM) has gained attention its chondro-inductive properties, yet dECM-based bioinks have limitations in mechanical stability printability. This study proposes a rapid light-based bioprinting method using tyrosine-based crosslinking mechanism, which does not require chemical modifications of dECM thereby preserves structure bioactivity. Combining this resin with Filamented Light (FLight) biofabrication enables the creation cellular, porous, anisotropic scaffolds composed aligned microfilaments. Specifically, we focus on effects various biopolymer compositions (i.e. hyaluronic acid, collagen I, dECM) inner bulk light vs FLight) immune response cell morphology, investigate their influence nascent ECM production long-term tissue maturation. Our findings highlight importance FLight directing deposition resembling promoting construct maturation, they emphasize superiority biological-rich over single-component materials cartilage, offering new avenues development effective strategies.
Язык: Английский
Процитировано
1