Tissue Engineering Part A, Journal Year: 2025, Volume and Issue: unknown, P. e151 - e300
Published: May 7, 2025
ACS Applied Polymer Materials, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 29, 2025
Language: Английский
Citations
3
Advances in Colloid and Interface Science, Journal Year: 2023, Volume and Issue: 321, P. 103030 - 103030
Published: Oct. 20, 2023
The impairment of articular cartilage due to traumatic incidents or osteoarthritis has posed significant challenges for healthcare practitioners, researchers, and individuals suffering from these conditions. Due the absence an approved treatment strategy complete restoration defects their native state, tissue condition often deteriorates over time, leading osteoarthritic (OA). However, recent advancements in field regenerative medicine have unveiled promising prospects through utilization injectable hydrogels. This versatile class biomaterials, characterized by ability emulate characteristics cartilage, offers distinct advantage minimally invasive administration directly site damage. These hydrogels can also serve as ideal delivery vehicles a diverse range bioactive agents, including growth factors, anti-inflammatory drugs, steroids, cells. controlled release such biologically active molecules hydrogel scaffolds accelerate healing, stimulate chondrogenesis, modulate inflammatory microenvironment halt progression. present review aims describe methods used design hydrogels, expound upon applications molecules, provide update on advances leveraging systems foster regeneration.
Language: Английский
Citations
39
Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: unknown
Published: July 28, 2023
Abstract Zwitterionic hydrogels have high potential for cartilage tissue engineering due to their ultra‐hydrophilicity, nonimmunogenicity, and superior antifouling properties. However, application in this field has been limited so far, the lack of injectable zwitterionic that allow encapsulation cells a biocompatible manner. Herein, novel strategy is developed engineer employing granular are injectable, self‐healing, situ crosslinkable direct with biocompatibility. The hydrogel produced by mechanical fragmentation bulk photocrosslinked made carboxybetaine acrylamide (CBAA), or mixture CBAA sulfobetaine methacrylate (SBMA). microgels enzymatically using horseradish peroxidase, quickly stabilize construct, resulting microporous hydrogel. Encapsulated human primary chondrocytes highly viable able proliferate, migrate, produce cartilaginous extracellular matrix (ECM) It also shown increasing porosity incorporation SBMA, cell proliferation ECM secretion further improved. This simple scalable method, which expanding versatility diverse applications.
Language: Английский
Citations
32
Biofabrication, Journal Year: 2024, Volume and Issue: 16(2), P. 025004 - 025004
Published: Jan. 4, 2024
Abstract Foreign body response (FBR) is a pervasive problem for biomaterials used in tissue engineering. Zwitterionic hydrogels have emerged as an effective solution to this problem, due their ultra-low fouling properties, which enable them effectively inhibit FBR vivo . However, no versatile zwitterionic bioink that allows high resolution extrusion bioprinting of implants has thus far been reported. In work, we introduce simple, novel method producing microgel bioink, using alginate methacrylate (AlgMA) crosslinker and mechanical fragmentation fabrication method. Photocrosslinked made carboxybetaine acrylamide (CBAA) sulfobetaine (SBMA) are mechanically fragmented through meshes with aperture diameters 50 90 µ m produce bioink. The bioinks both sizes showed excellent rheological properties were high-resolution printing objects overhanging features without requiring support structure or bath. AlgMA dual role, allowing primary photocrosslinking the bulk hydrogel well secondary ionic crosslinking produced microgels, quickly stabilize printed construct calcium bath microporous scaffold. Scaffolds ∼20% porosity, they supported viability chondrogenesis encapsulated human chondrocytes. Finally, meniscus model was bioprinted, demonstrate bioink’s versatility at large, cell-laden constructs stable further vitro culture promote cartilaginous production. This easy scalable strategy direct cell encapsulation scaffold potential biocompatibility nature
Language: Английский
Citations
16
Nano-Micro Letters, Journal Year: 2024, Volume and Issue: 16(1)
Published: June 17, 2024
Abstract Microgels prepared from natural or synthetic hydrogel materials have aroused extensive attention as multifunctional cells drug carriers, that are promising for tissue engineering and regenerative medicine. can also be aggregated into microporous scaffolds, promoting cell infiltration proliferation repair. This review gives an overview of recent developments in the fabrication techniques applications microgels. A series conventional novel strategies including emulsification, microfluidic, lithography, electrospray, centrifugation, gas-shearing, three-dimensional bioprinting, etc. discussed depth. The characteristics microgels microgel-based scaffolds culture delivery elaborated with emphasis on advantages these carriers therapy. Additionally, we expound ongoing foreseeable current limitations their aggregate field biomedical engineering. Through stimulating innovative ideas, present paves new avenues expanding application techniques.
Language: Английский
Citations
15
Macromolecular Rapid Communications, Journal Year: 2024, Volume and Issue: 45(11)
Published: Feb. 24, 2024
Abstract Hydrogels, key in biomedical research for their hydrophilicity and versatility, have evolved with hydrogel microspheres (HMs) of micron‐scale dimensions, enhancing role minimally invasive therapeutic delivery, tissue repair, regeneration. The recent emergence nanomaterials has ushered a revolutionary transformation the field, which demonstrates tremendous potential targeted therapies, biological imaging, disease diagnostics. Consequently, integration advanced nanotechnology promises to trigger new revolution realm hydrogels. HMs loaded combine advantages both hydrogels nanomaterials, enables multifaceted functionalities such as efficient drug sustained release, therapy, lubrication, biochemical detection, medical biosensing monitoring, micro‐robotics. Here, this review comprehensively expounds upon commonly used classifications. Then, it provides comprehensive insights into raw materials preparation methods HMs. Besides, common strategies employed achieve nano‐micron combinations are summarized, latest applications these combined field elucidated. Finally, valuable future design development provided.
Language: Английский
Citations
11
Carbohydrate Polymers, Journal Year: 2025, Volume and Issue: 353, P. 123277 - 123277
Published: Jan. 14, 2025
Language: Английский
Citations
1
Bioactive Materials, Journal Year: 2025, Volume and Issue: 48, P. 273 - 293
Published: Feb. 20, 2025
Language: Английский
Citations
1
Nano Select, Journal Year: 2025, Volume and Issue: unknown
Published: March 4, 2025
ABSTRACT To expand the use of collagen‐based biomaterials beyond their current applications in three‐dimensional (3D) cell culture, tissue engineering, and biofabrication, limitations such as poor shear‐thinning behavior control over porosity during gelation need to be overcome. Granular promise address these constraints, however uniform scalable preparation from extracellular matrix materials is challenging. this need, we employed a droplet microfluidic approach prepared irregularly shaped microgels fibrillar collagen collagen‐glycosaminoglycan (GAG) copolymer continuous oil phase, at rates up 5500 s −1 . The allowed us tune average microgel size 40 170 µm. Microgels obtained after removal phase were found promote attachment proliferation human fibroblasts mesenchymal stromal/stem cells. with packing densities exceeding 65 vol% exhibited rheological behavior, requirement for injectable bioinks. Cell‐containing granular contracted 2.8 times less than thermally gelled matrices comparable concentration. In case study, skin model fibroblast containing collagen‐GAG (CG) layer covered an epithelium revealed immunohistochemical markers associated intact month‐long air–liquid interface (ALI) culture.
Language: Английский
Citations
1
Biofabrication, Journal Year: 2024, Volume and Issue: 16(2), P. 025009 - 025009
Published: Jan. 10, 2024
Three-dimensional (3D) bioprinting embedded within a microgel bath has emerged as promising strategy for creating intricate biomimetic scaffolds. However, it remains great challenge to construct tissue-scale structures with high resolution by using 3D due the large particle size and polydispersity of medium, well its limited cytocompatibility. To address these issues, novel uniform sub-microgels cell-friendly cationic-crosslinked kappa-carrageenan (κ-Car) are developed through an easy-to-operate mechanical grinding strategy. Theseκ-Car maintain submicron around 642 nm display rapid jamming-unjamming transition 5 s, along excellent shear-thinning self-healing properties, which critical fidelity in construction tissue architecture via bioprinting. Utilizing this new sub-microgel various organ structures, including heart, lungs, trachea, branched vasculature, kidney, auricle, nose, liver, successfully fabricated delicate fine shape fidelity. Moreover, bone marrow mesenchymal stem cells encapsulated printed constructs exhibit remarkable viability exceeding 92.1% robust growth. Thisκ-Car medium offers innovative avenue achieving high-quality bioprinting, facilitating fabrication functional biological structural organizations.
Language: Английский
Citations
8