Current Opinion in Cell Biology,
Journal Year:
2021,
Volume and Issue:
72, P. 81 - 90
Published: July 28, 2021
Collective
behaviour
emerges
from
interacting
units
within
communities,
such
as
migrating
herds,
swimming
fish
schools,
and
cells
tissues.
At
the
microscopic
level,
collective
behaviours
include
cell
migration
in
development
cancer
invasion,
rhythmic
gene
expression
pattern
formation,
competition
homeostasis
cancer,
force
generation
mechano-sensing
morphogenesis.
Studying
initiation
maintenance
of
is
key
to
understand
principles
development,
regeneration
disease.
However,
manifold
influences
contributing
factors
vivo
environments
challenge
dissection
causalities
animal
models.
As
an
alternative
model
that
has
emerged
overcome
this
difficulty,
vitro
three-dimensional
organoid
cultures
provide
a
reductionist
approach
yet
retain
similarities
with
tissue
cellular
composition
organisation.
Here,
we
focus
on
recent
progresses
studying
different
systems
discuss
their
advantages
possibility
improvement
for
future
applications.
Science Advances,
Journal Year:
2020,
Volume and Issue:
6(12)
Published: March 20, 2020
The
integration
of
structure
and
function
for
tissue
engineering
scaffolds
is
great
importance
in
mimicking
native
bone
tissue.
However,
the
complexity
hierarchical
structures,
requirement
mechanical
properties,
diversity
resident
cells
are
major
challenges
constructing
biomimetic
scaffolds.
Herein,
a
Haversian
bone-mimicking
scaffold
with
integrated
was
successfully
prepared
via
digital
laser
processing
(DLP)-based
3D
printing.
compressive
strength
porosity
could
be
well
controlled
by
altering
parameters
structure.
showed
potential
multicellular
delivery
inducing
osteogenic,
angiogenic,
neurogenic
differentiation
vitro
accelerated
ingrowth
blood
vessels
new
formation
vivo.
work
offers
strategy
designing
structured
functionalized
biomaterials
through
complex
regeneration.
Nature Communications,
Journal Year:
2021,
Volume and Issue:
12(1)
Published: Oct. 8, 2021
Abstract
Parkinson’s
disease
and
related
synucleinopathies
are
characterized
by
the
abnormal
accumulation
of
alpha-synuclein
aggregates,
loss
dopaminergic
neurons,
gliosis
substantia
nigra.
Although
clinical
evidence
in
vitro
studies
indicate
disruption
Blood-Brain
Barrier
disease,
mechanisms
mediating
endothelial
dysfunction
is
not
well
understood.
Here
we
leveraged
Organs-on-Chips
technology
to
develop
a
human
Brain-Chip
representative
nigra
area
brain
containing
astrocytes,
microglia,
pericytes,
microvascular
cells,
cultured
under
fluid
flow.
Our
αSyn
fibril-induced
model
was
capable
reproducing
several
key
aspects
including
phosphorylated
(pSer129-αSyn),
mitochondrial
impairment,
neuroinflammation,
compromised
barrier
function.
This
may
enable
research
into
dynamics
cell-cell
interactions
serve
as
testing
platform
for
target
identification
validation
novel
therapeutics.
Small,
Journal Year:
2019,
Volume and Issue:
16(9)
Published: Oct. 11, 2019
Fabrication
of
artificial
biomimetic
materials
has
attracted
abundant
attention.
As
one
the
subcategories
materials,
cells
are
highly
significant
for
multiple
disciplines
and
their
synthesis
been
intensively
pursued.
In
order
to
manufacture
robust
"alive"
with
high
throughput,
easy
operation,
precise
control,
flexible
microfluidic
techniques
widely
utilized.
Herein,
recent
advances
in
microfluidic-based
methods
droplets,
vesicles,
summarized.
First,
droplet
fabrication
manipulation
on
T-junction,
flow-focusing,
coflowing
devices
discussed.
Then,
formation
unicompartmental
multicompartmental
vesicles
based
microfluidics
Furthermore,
engineering
droplet-based
vesicle-based
by
is
also
reviewed.
Moreover,
applied
imitating
cell
behavior
acting
as
bioreactors
synthetic
biology
highlighted.
Finally,
current
challenges
future
trends
This
review
should
be
helpful
researchers
fields
microfluidics,
biomaterial
fabrication,
biology.
Advanced Healthcare Materials,
Journal Year:
2021,
Volume and Issue:
10(6)
Published: Feb. 2, 2021
Over
the
past
few
years,
mesenchymal
stem
(or
stromal)
cells
(MSCs)
have
garnered
enormous
interest
due
to
their
therapeutic
value
especially
for
multilineage
differentiation
potential
leading
regenerative
medicine
applications.
MSCs
undergo
physiological
changes
upon
in
vitro
expansion
resulting
expression
of
different
receptors,
thereby
inducing
high
variabilities
efficacy.
Therefore,
understanding
biochemical
cues
that
influence
native
local
signals
on
or
proliferation
these
is
very
important.
There
been
several
reports
culture
low
oxygen
gradient
hypoxic
conditions)
upregulates
stemness
markers
and
promotes
cell
an
undifferentiated
state,
as
hypoxia
mimics
conditions
progenitor
experience
within
tissue.
However,
studies
report
gradients
causing
ambiguity
interpretation
results.
In
this
progress
report,
it
aimed
summarize
recent
field
with
specific
focus
conflicting
results
reported
during
application
improving
MSCs.
Further,
tried
decipher
factors
can
affect
characteristics
MSC
under
suggest
a
techniques
could
be
combined
better
recapitulate
tissue
niche.
Advanced Materials,
Journal Year:
2022,
Volume and Issue:
34(23)
Published: Feb. 23, 2022
Abstract
Bio‐hybrid
technologies
aim
to
replicate
the
unique
capabilities
of
biological
systems
that
could
surpass
advanced
artificial
technologies.
Soft
bio‐hybrid
robots
consist
synthetic
and
living
materials
have
potential
self‐assemble,
regenerate,
work
autonomously,
interact
safely
with
other
species
environment.
Cells
require
a
sufficient
exchange
nutrients
gases,
which
is
guaranteed
by
convection
diffusive
transport
through
liquid
media.
The
functional
development
long‐term
survival
tissues
in
vitro
can
be
improved
dynamic
flow
culture,
but
only
microfluidic
control
develop
tissue
fine
structuring
regulation
at
microscale.
Full
growth
microscale
will
eventually
lead
macroscale
constructs,
are
needed
as
component
soft
This
review
summarizes
recent
progress
techniques
engineer
tissues,
focusing
on
use
muscle
cells
for
robotic
bio‐actuation.
Moreover,
instances
bio‐actuation
greatly
benefit
from
fusion
microfluidics
highlighted,
include:
microfabrication
matrices,
biomimicry
cell
microenvironments,
maturation,
perfusion,
vascularization.
Advanced Functional Materials,
Journal Year:
2023,
Volume and Issue:
33(32)
Published: April 25, 2023
Abstract
Microfluidics
and
bioinspired
superwetting
materials,
as
two
crucial
branches
of
scientific
research,
are
entering
their
golden
age
development.
As
an
emerging
interdisciplinary
subject
these
fields,
open
microfluidics
is
triggering
technological
revolutions
in
many
disciplines,
including
rapid
medical
diagnosis,
biochemical
analysis,
liquid
manipulation,
3D
printing,
etc.
However,
this
new
research
area
has
yet
to
attract
extensive
attention.
So,
a
timely
review
necessary
organize
the
development
process,
summarize
current
achievements,
discuss
challenges
or
chances
for
ongoing
trend.
In
review,
evolution
from
closed
combed
first.
Then,
three
typical
systems
introduced
emphatically.
Based
on
this,
divided
into
different
categories
according
bionic
objects
focus
study.
Taking
natural
phenomena
entry
point,
underlying
mechanism
application
systematically
discussed
summarized.
Several
applications
also
mentioned.
Finally,
some
views
major
problems,
existing
challenges,
developing
trends
briefly
put
forward
field
guide
future
research.
Cell,
Journal Year:
2023,
Volume and Issue:
186(10), P. 2092 - 2110.e23
Published: May 1, 2023
The
third
and
fourth
weeks
of
gestation
in
primates
are
marked
by
several
developmental
milestones,
including
gastrulation
the
formation
organ
primordia.
However,
our
understanding
this
period
is
limited
due
to
restricted
access
vivo
embryos.
To
address
gap,
we
developed
an
embedded
3D
culture
system
that
allows
for
extended
ex
utero
cynomolgus
monkey
embryos
up
25
days
post-fertilization.
Morphological,
histological,
single-cell
RNA-sequencing
analyses
demonstrate
cultured
largely
recapitulated
key
events
development.
With
platform,
were
able
delineate
lineage
trajectories
genetic
programs
involved
neural
induction,
lateral
plate
mesoderm
differentiation,
yolk
sac
hematopoiesis,
primitive
gut,
primordial
germ-cell-like
cell
development
monkeys.
Our
provides
a
robust
reproducible
platform
growing
from
blastocysts
early
organogenesis
studying
primate
embryogenesis
utero.
Engineered Regeneration,
Journal Year:
2022,
Volume and Issue:
3(1), P. 110 - 120
Published: Jan. 8, 2022
The
construction
of
biological
scaffolds
in
vitro
plays
an
important
role
regenerative
engineering,
which
has
always
been
the
focus
research.
application
scaffold
materials
combined
with
cells/bioactive
factors
is
considered
to
have
great
potential
for
tissue
regeneration.
Various
strategies
used
constructing
scaffolds,
including
electrospinning/spraying,
direct
laser
writing,
solvent
casting,
and
microfluidic
technology.
Among
them,
considering
advantages
safety,
low
cost,
highly
controllable
processing
characteristics,
technology
become
a
promising
method
scaffolds.
This
review
overviews
recent
research
progress
bioscaffolds
engineering.
Firstly,
we
introduce
some
typical
natural
polymers
are
widely
construct
bioscaffolds.
Then,
different
structures
templated
by
droplets
fibers
described.
Furtherly,
talk
about
applications
wound
healing,
drug
delivery,
bone
regeneration,
nerve
Finally,
challenges
future
prospects
development
engineering
discussed.
