Light-based vat-polymerization bioprinting

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: June 22, 2023

Language: Английский

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Toward Multiscale, Multimaterial 3D Printing

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(34)

Published: May 22, 2024

Biological materials and organisms possess the fundamental ability to self-organize, through which different components are assembled from molecular level up hierarchical structures with superior mechanical properties multifunctionalities. These complex composites inspire material scientists design new engineered by integrating multiple ingredients over a wide range. Additive manufacturing, also known as 3D printing, has advantages respect fabricating multiscale multi-material structures. The need for multifunctional is driving printing techniques toward arbitrary architectures next of complexity. In this paper, aim highlight key features those that can produce either or multimaterial structures, including innovations in methods, processing approaches, hardware improvements. Several issues challenges related current methods discussed. Ultimately, authors provide their perspective on how realize combination capabilities processes future directions based emerging research.

Language: Английский

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A Perfusable Multi‐Hydrogel Vasculature On‐Chip Engineered by 2‐Photon 3D Printing and Scaffold Molding to Improve Microfabrication Fidelity in Hydrogels

Advanced Materials Technologies, Journal Year: 2024, Volume and Issue: 9(4)

Published: Jan. 4, 2024

Abstract Engineering vasculature networks in physiologically relevant hydrogels represents a challenge terms of both fabrication, due to the cell–bioink interactions, as well subsequent hydrogel‐device interfacing. Here, new cell‐friendly fabrication strategy is presented realize perfusable multi‐hydrogel models supporting co‐culture integrated microfluidic chip. The system comprises two different specifically support growth and proliferation cell types selected for vessel model. First, channels are printed gelatin‐based ink by two‐photon polymerization (2PP) inside device. Then, human lung fibroblast‐laden fibrin hydrogel injected surround network. Finally, endothelial cells seeded channels. printing parameters composition optimized reduce swelling ensure stable model that can be perfused with media. Fabricating structure steps ensures no exposed cytotoxic processes, while still obtaining high fidelity printing. In this work, possibility guide invasion through 3D scaffold perfusion 10 days successfully demonstrated on custom‐made system.

Language: Английский

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Light from Afield: Fast, High-Resolution, and Layer-Free Deep Vat 3D Printing

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(14), P. 8787 - 8822

Published: July 5, 2024

Harnessing light for cross-linking of photoresponsive materials has revolutionized the field 3D printing. A wide variety techniques leveraging broad-spectrum shaping have been introduced as a way to achieve fast and high-resolution printing, with applications ranging from simple prototypes biomimetic engineered tissues regenerative medicine. Conventional light-based printing use material in layer-by-layer fashion produce complex parts. Only recently, new emerged which deploy multidirection, tomographic, light-sheet or filamented image projections deep into volume resin-filled vat photoinitiation cross-linking. These Deep Vat (DVP) approaches alleviate need layer-wise enable unprecedented fabrication speeds (within few seconds) high resolution (>10 μm). Here, we elucidate physics chemistry these processes, their commonalities differences, well emerging biomedical non-biomedical fields. Importantly, highlight limitations, future scope research that will improve scalability applicability DVP engineering medicine applications.

Language: Английский

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Embedding Biomimetic Vascular Networks via Coaxial Sacrificial Writing into Functional Tissue

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 2, 2024

Printing human tissues and organs replete with biomimetic vascular networks is of growing interest. While it possible to embed perfusable channels within acellular densely cellular matrices, they do not currently possess the architectures found in native vessels. Here, coaxial sacrificial writing into functional (co-SWIFT) developed, an embedded bioprinting method capable generating hierarchically branching, multilayered both granular hydrogel matrices. Coaxial printheads are designed extended core-shell configuration facilitate robust core-core shell-shell interconnections between printed branching vessels during bioprinting. Using optimized ink combinations, composed a smooth muscle cell-laden shell that surrounds lumens coaxially matrices of: 1) transparent alginate microparticles, 2) microparticle-laden collagen, or 3) cardiac spheroids derived from induced pluripotent stem cells. Biomimetic blood exhibit good barrier function produced by seeding these interconnected confluent layer endothelial Importantly, co-SWIFT mature under perfusion, beat synchronously, cardio-effective drug response vitro. This advance opens new avenues for scalable biomanufacturing vascularized organ-specific testing, disease modeling, therapeutic use.

Language: Английский

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Biofabrication of anisotropic articular cartilage based on decellularized extracellular matrix

Biofabrication, Journal Year: 2025, Volume and Issue: 17(1), P. 015044 - 015044

Published: Jan. 1, 2025

Tissue-engineered grafts that mimic articular cartilage show promise for treating injuries. However, engineering cell-based therapies to match zonal architecture and biochemical composition remains challenging. Decellularized extracellular matrix (dECM) has gained attention its chondro-inductive properties, yet dECM-based bioinks have limitations in mechanical stability printability. This study proposes a rapid light-based bioprinting method using tyrosine-based crosslinking mechanism, which does not require chemical modifications of dECM thereby preserves structure bioactivity. Combining this resin with Filamented Light (FLight) biofabrication enables the creation cellular, porous, anisotropic scaffolds composed aligned microfilaments. Specifically, we focus on effects various biopolymer compositions (i.e. hyaluronic acid, collagen I, dECM) inner bulk light vs FLight) immune response cell morphology, investigate their influence nascent ECM production long-term tissue maturation. Our findings highlight importance FLight directing deposition resembling promoting construct maturation, they emphasize superiority biological-rich over single-component materials cartilage, offering new avenues development effective strategies.

Language: Английский

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From pixels to voxels: A mechanistic perspective on volumetric 3D-printing

Progress in Polymer Science, Journal Year: 2023, Volume and Issue: 147, P. 101755 - 101755

Published: Oct. 20, 2023

Language: Английский

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Current advances in engineering meniscal tissues: insights into 3D printing, injectable hydrogels and physical stimulation based strategies

Biofabrication, Journal Year: 2024, Volume and Issue: 16(2), P. 022006 - 022006

Published: Jan. 26, 2024

Abstract The knee meniscus is the cushioning fibro-cartilage tissue present in between femoral condyles and tibial plateau of joint. It largely avascular nature suffers from a wide range tears injuries caused by accidents, trauma, active lifestyle populace old age individuals. Healing especially difficult due to its avascularity hence requires invasive arthroscopic approaches such as surgical resection, suturing or implantation. Though various engineering are proposed for treatment tears, three-dimensional (3D) printing/bioprinting, injectable hydrogels physical stimulation involving modalities gaining forefront past decade. A plethora new printing direct light photopolymerization volumetric printing, biomaterials loaded with growth factors low-intensity ultrasound being added portfolio along contemporary tear mitigation measures. This review discusses on necessary design considerations, 3D modeling practices meniscal treatments within scope regeneration. Also, suitable materials, cell sources, factors, fixation lubrication strategies, mechanical approaches, strategies management have been elaborated. We also summarized potential technologies framework that could be herald future repair approaches.

Language: Английский

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Thermal Shrinking of Biopolymeric Hydrogels for High Resolution 3D Printing of Kidney Tubules

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 2, 2024

Abstract The effective replication of microtubular structures in tissue engineering remains a great challenge. In this study, the temperature‐responsive characteristics poly( N ‐isopropylacrylamide) (pNIPAM) to create intricate, high‐resolution tubular through shrinking mechanism is investigated by exploring 2 thermosensitive hydrogels–gelatin methacryloyl (gelMA) and silk fibroin (silkMA)–combined with pNIPAM. Systematic investigations revealed precise control behavior at elevated temperatures (33–37 °C) as function polymer concentration. hydrogel sizes reduce ≈15% from room temperature (RT) 33 °C ≈40% RT 37 for both types. affects mechanical properties, increasing compressive modulus ≈2.8‐fold gelMA‐pNIPAM gels ≈5.1‐fold silkMA‐pNIPAM °C. Combined volumetric printing, these materials achieve resolution enhancements ≈20% positive features ≈70% negative features, enabling creation complex, within seconds, open channels (≈50 µm). GelMA‐pNIPAM hydrogels show better cell compatibility compared hydrogels, promoting adhesion viability. This study demonstrates hydrogels' capability engineer printing–an efficient route fabricate microenvironments mimicking native tissues potential developing relevant vitro models.

Language: Английский

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Lithography-based 3D printing of hydrogels

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Language: Английский

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